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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for GA binding protein alpha chain GABPA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: All samples were prepared in low protein-binding microcentrifuge tubes (ThermoFisher Scientific, Waltham, MA, USA).
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bioRxiv - Bioengineering 2021Quote: ... Non-specific antibody binding was prevented by incubating with 2% BSA (Thermo Fisher) for 2 h ...
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bioRxiv - Developmental Biology 2022Quote: ... and non-specific antibody binding was minimised by blocking with CAS block (Invitrogen) for at least 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Successful binding was analyzed using anti-His Tag antibody (PCRD, Invitrogen #MA1-135) or anti-Myc Tag (DCRD ...
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bioRxiv - Biochemistry 2020Quote: A 5-mL HiTrap™ Protein G HP column (Cytiva) was equilibrated with 10 CVs of Protein G IgG Binding Buffer (pH 5.0; Thermo Fisher Scientific). Spent medium containing CR3022 mAb was diluted with binding buffer at a 1:1 volume ratio and filtered using a 0.22 μm stericup ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then incubated in cardiac troponin T antibody (MS-295-P1, Epredia) diluted 1:400 in PBST or alpha-actinin antibody (MA1-22863, Invitrogen) diluted 1:200 in PBST overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: Alexa 647 labelled Goat anti-Rabbit IgG secondary antibody and anti-alpha tubulin primary antibody was purchased from Thermo Fisher Scientific (Australia) ...
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bioRxiv - Physiology 2024Quote: ... and incubated overnight at 4°C in primary antibodies (1:500 anti-NALCN, Alomone labs; 1:4000 anti-SLO2.1, Absolute Antibody; 1:50 alpha-Smooth muscle actin, Invitrogen). Slides were incubated in biotinylated secondary antibodies in 10% GS for 30 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Anti-IgM antibody (µ-chain specific) was monobiotinylated and labeled with Alexa Fluor 647 (Thermo Fisher) as described previously (18) ...
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bioRxiv - Bioengineering 2022Quote: ... The serum-free medium used for encapsulation was alpha minimum essential medium (alpha-MEM, Gibco) supplemented with 10 % bovine serum albumin (BSA ...
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bioRxiv - Developmental Biology 2023Quote: ... sorted cells were cultured on irradiated OP9DLL1 monolayers in Alpha-MEM (Alpha-MEM, ThermoFisher, 12000063) supplemented with 20% FBS (HyClone) ...
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bioRxiv - Microbiology 2020Quote: ... Binding of primary antibodies were visualized using Alexa 488 α-rabbit and 568 α-mouse secondary antibodies (Invitrogen). The S and HN proteins expression were analysed through fluorescence for wild-type and recombinant viruses compared to mock infected cells ...
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bioRxiv - Microbiology 2023Quote: ... Binding of primary antibody was visualized using Alexa 488 α-rabbit or 568 α-mouse secondary antibody (Invitrogen). 4’,6-Diamidino-2-phenylindole (DAPI ...
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bioRxiv - Physiology 2024Quote: Immunoprecipitation of proteins without the contaminant of antibody heavy and light chain through bead antibody conjugation was carried out using the Pierce Co-IP kit (Invitrogen #26149). Briefly ...
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bioRxiv - Bioengineering 2022Quote: ... and Myosin Heavy Chain (ThermoFisher #Bt03273061). Reactions were performed according to the manufacturer’s instructions on a Bio-Rad CFX96 Real Time System thermocycler ...
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bioRxiv - Neuroscience 2024Quote: The following primary antibodies were used for Western blotting: mouse alpha tubulin (Invitrogen DM1A, 1:2000), mouse GluA2 (Millipore MAB397 ...
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bioRxiv - Cell Biology 2024Quote: The following primary antibodies were used: rat anti-mouse alpha-tubulin (YL1/2, Invitrogen, 1:500), mouse anti-mouse acetylated-tubulin (6-11B-1 ...
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bioRxiv - Molecular Biology 2020Quote: ... The sheath gas pressure and the auxiliary gas pressure were 30 and 5 (Thermo Fisher arbitrary units) respectively ...
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bioRxiv - Immunology 2021Quote: ... The media was diluted with an equal volume of Protein A IgG Binding Buffer (Thermo Scientific) and loaded onto the column ...
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bioRxiv - Physiology 2022Quote: ... 18S ribosomal RNA (18S) and TATA-binding protein (Tbp).The primers (Thermofisher Scientific, Waltham, MA, USA) were derived from mouse sequences and are listed in supplemental methods (supplement table 2.).
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bioRxiv - Molecular Biology 2021Quote: Putative ac4C binding proteins were subjected to SDS PAGE on Novex Tris-Glycine 4-20% (Invitrogen) gel followed by either silver staining with Pierce Silver Stain Kit (Thermo ...
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bioRxiv - Immunology 2021Quote: ... S protein binding was detected using 25 µL/well of PNPP (p-nitrophenyl phosphate;Thermo Fisher) and the reaction was measured at a wavelength of 405 nm by the Varioskan Lux Reader (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA-protein binding reactions were performed according to the manufacturer’s instructions (Thermo Fisher Scientific, USA). The 4% polyacryl gel was used to separate the DNA-protein complexes ...
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bioRxiv - Cell Biology 2020Quote: ... KKT4 proteins were buffer-exchanged into the binding buffer using Zeba spin desalting columns (Thermo Fisher). KKT42–645 (0.67 µM ...
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bioRxiv - Immunology 2021Quote: Nunc MaxiSorpTM high protein-binding capacity 96 well plates (Thermo Fisher Scientific, Cat# 44-2404-21) were coated overnight at 4 °C with 1µg/ml SARS-CoV-2 RBD ...
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bioRxiv - Bioengineering 2020Quote: ... The flavin binding fluorescent protein gene was synthesized from previously known sequence (56) (Thermo Fisher Scientific) and the plasmid was formed using the hot fusion method ...
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bioRxiv - Immunology 2022Quote: ... Supernatant with positive RBD binding signals was next purified using Protein A/G beads (Thermo Scientific), concentrated using a 30 kDa or 100 kDa cut-off concentrator (Vivaspin ...
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bioRxiv - Cell Biology 2023Quote: All steps from here on were performed in Low protein binding microcentrifuge tubes (Thermo Scientific, 90410). Bead preparation and antibody binding ...
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bioRxiv - Microbiology 2024Quote: Pcas-binding proteins were pulled-down with the streptavidin beads (Invitrogen Dynabeads™ M-270, 65305) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... 5 mM MgCl2) for cell membrane binding proteins (LPL, Caveolin 1) and quantified with Pierce™ BCA Protein Assay Kit (Buffer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: rox2RNA probes at 100 nM and hrp38 RNA probes at 50nM were incubated with MBP-tagged FL CLAMPWT protein or MBP-tagged FL CLAMPdelPrLD protein in REMSA binding buffer provided with the LightShift Chemiluminescent RNA EMSA kit (Thermo Scientific, USA) at room temperature for 30 min according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Nanobody binding was detected using Myc-tag specific antibody (2 μg/ml – Life Technologies) and then anti-mouse-Alexa Fluor 700 (1.3 μg/ml – Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Nonspecific antibody binding was blocked with a 10% Goat Serum (Thermo Fisher (Waltham, MA)) overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... IBA-1 (ionized calcium-binding adapter molecule 1; antibody PA5-21274, Invitrogen; 1:150) and astrocytes (anti-S100 beta antibody ...
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bioRxiv - Immunology 2023Quote: ... Antibody binding was detected either with mouse-anti-human or rabbit IgG-PE (Invitrogen) and detected using the FACS Symphony (BD) ...
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bioRxiv - Microbiology 2024Quote: ... Antibody binding was detected using Super Signal West Pico chemiluminescence substrate (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2024Quote: Immunoglobulin heavy chain (HC) and light chain (LC - kappa) encoding DNA sequences were synthesised (Thermo Fisher, UK) and cloned into a dual chain expression vector with the human cytomegalovirus (CMV ...
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bioRxiv - Immunology 2024Quote: ... The sequence confirmed Fab heavy chain and light chain plasmids were co-transfected into Expi293F cells (Gibco) for expression and purified with HisPur Ni-NTA resin (Thermo Scientific).
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bioRxiv - Synthetic Biology 2021Quote: ... alpha-pinene (Acros Organics, CAT#: 131270050); limonene (Tokyo Chemical Industry ...
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bioRxiv - Cell Biology 2021Quote: ... Alpha-Tubulin (MS581P1) from Thermo Fisher, SEC23B (GTX82432 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1:3000 alpha-bungarotoxin 488 (Invitrogen), 1:1000 rabbit anti-Syntaxin1 (gift of Thomas Südhof ...
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bioRxiv - Immunology 2020Quote: ... and Interferon alpha (Invitrogen, Cat. BMS6027TWO). Plates were read using an Epoch BioTek Gen5 microplate reader at 450nm ...
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bioRxiv - Microbiology 2022Quote: ... CD8-alpha Pacific Orange (5H10, ThermoFisher), CD11b PerCP-Cy5.5 (M1/70) ...
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bioRxiv - Systems Biology 2022Quote: ... TNFα (tumor necrosis factor-alpha) (ThermoFisher), PGE2 (prostaglandin E2 ...
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bioRxiv - Physiology 2024Quote: ... and alpha-smooth muscle actin (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... alpha modification (MEM α; Life Technologies), supplemented with 15% FBS ...
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bioRxiv - Genetics 2024Quote: ... and 30% Mem Alpha (Gibco, 12571063). Cells were grown in an incubator at 37°C with 5% CO2.
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bioRxiv - Developmental Biology 2020Quote: ... Corresponding Alexa-Fluor (−488, −568 and −647) secondary antibodies were used to reveal primary antibody binding (Thermo Fisher Scientific). For generating the plots shown in Figure 1d ...
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bioRxiv - Immunology 2021Quote: DNA constructs encoding anti-SCARB2 antibody heavy and light chain variable domains (clone JL278) were synthesized (ThermoFisher) and cloned into mammalian expression vectors containing a mouse IGHV signal peptide and IgG1 constant regions ...
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bioRxiv - Microbiology 2023Quote: Hydrogen gas production was measured on a Fisher Scientific TRACE 1300 Gas Chromatograph (Thermo Fisher Scientific, Waltham, MA) using a TRACE TR-5 GC Column (part no ...