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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... or separating the pooled samples on a 4% agarose gel (E-Gel® EX Agarose Gels, 4%, Invitrogen), and gel purification of fragments larger than adapter dimers (>150bp) ...
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bioRxiv - Biochemistry 2020Quote: CHO-K1 cells in 10-cm dishes were transfected with complementary DNA encoding FLAG-tagged mLPCAT2 or one of several FLAG-tagged mLPCAT2 mutants using Lipofectamine 2000 (Thermo Fisher Scientific, USA). At 48 h post-transfection ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were then stained with M2 FLAG primary antibody (SigmaAldrich, F1804) and AlexFluor 594-conjugated anti-mouse goat antibody (ThermoFisher, A-11005) using the same conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... an anti-Flag antibody or normal mouse IgG were added to the nuclear extract together with Dynabeads bearing anti-Mouse IgG (Thermo Fisher Scientific). After incubation for 1 h with gradual rotation at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... Samples were fixed with 4% buffered paraformaldehyde at 24 hpi and stained with Monoclonal anti-Flag M2 antibody (1:500, Sigma, Thermo Scientific™) and alexa 488 anti-mouse secondary antibody to visualize expressing Chlamydia ...
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bioRxiv - Developmental Biology 2022Quote: ... The membrane was washed 5 times 5 min in TBS-T prior to overnight incubation at 4°C with 1° anti-Flag antibody (1:500, ThermoFisher, PA1-984B). The next morning ...
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bioRxiv - Pathology 2023Quote: ... The conjugation solution was prepared according to the manufacturer’s instructions with the anti-FLAG Monoclonal Antibody (FG4R) at 1:3,000 dilution (ThermoFisher Scientific, no. MA1-91878). One ml functionalized NP was centrifugated at 10,000 rpm for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... Samples were then washed with filtered PBS and stained with DAPI to visualize DNA and monoclonal anti-FLAG M2 antibody (Sigma, Thermo Scientific™) with alexa 647 anti-mouse secondary antibody to visualize FtsI-3XFLAG expression ...
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bioRxiv - Biochemistry 2023Quote: ... cells were permeabilized and blocked for 1 h with DPBS containing 2% BSA and 0.1% Triton X-100 and then were incubated with 4 μg/mL anti-FLAG antibody diluted with 2% BSA in PBST with 0.1% Tween-20 (ThermoFisher Scientific, Cat# 85113) at 4 °C overnight ...
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bioRxiv - Microbiology 2019Quote: ... and gel purified using GeneJET Gel Extraction Kit (Thermo Fisher Scientific). The purified DNA was used as template to perform the 2nd step PCR using NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1 ...
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bioRxiv - Cell Biology 2022Quote: ... gel electrophoresis was performed using 4-12% Novex NuPage gels (Invitrogen #NP0322BOX ...
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bioRxiv - Neuroscience 2020Quote: ... Gradient gels (3% - 8% Tris-acetate protein gels, Thermo Fisher Scientific) were loaded with the lysates and run for 55 min at 150 V in Tris-tricine buffer (50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... The gel was stained using SYBR Safe DNA Gel Stain (Invitrogen), and three bands ...
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bioRxiv - Microbiology 2021Quote: ... Gels were transferred using the iBlot 2 Gel Transfer Device (Invitrogen). Membranes were blocked with 5% BSA in TBST then probed with primary antibodies for HMGCR (Ms mAb ...
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bioRxiv - Biochemistry 2020Quote: ... or native gels (NativePAGE 4-16% Bis-Tris Gel, Life Technologies) and transferred onto a nitrocellulose membrane (iBlot® 2 NC Mini Stacks ...
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bioRxiv - Cell Biology 2022Quote: ... and gel purified with GeneJET™ gel extraction kit (Thermo Fisher). Oligos were designed for the following target sequences and annealed (NAP1 ...
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bioRxiv - Genetics 2019Quote: ... The gel was stained using SYBR Safe DNA gel stain (Invitrogen Life Technologies ...
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bioRxiv - Developmental Biology 2019Quote: ... separated on a 4%-12% gradient gel (Invitrogen, Bolt gels, NW04122BOX), transferred to a PDVF membrane ...
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bioRxiv - Microbiology 2019Quote: ... agarose gel electrophoresis and the GeneJET Gel extraction kit (ThermoFisher Scientific) to purify the synthetic insert ...
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bioRxiv - Cancer Biology 2021Quote: ... The gels were stained with SYBR Safe DNA gel stain (Invitrogen). A Low DNA Mass Ladder (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... The final product was gel-purified (ThermoFisher GeneJET Gel Extraction Kit), digested with the restriction enzymes and cloned into each vector ...
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bioRxiv - Zoology 2023Quote: ... A gel apparatus (Mini Gel Tank, Thermo Fisher Scientific, Waltham, Massachusetts) was loaded with two 4-12% Bis-Tris gels (Bolt™ ...
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA integrity was validated using gel electrophoresis (E-Gel 2%, Invitrogen) and the ribosomal RNA 18S vs ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were fractionated by gel electrophoresis in 16% Tricine gels (Invitrogen) and stained with Coomassie brilliant blue ...
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bioRxiv - Microbiology 2023Quote: ... gel electrophoresis with SYBR Safe DNA Gel Stain (Invitrogen, USA, S33102), and 1 kb DNA ladders (New England Biolabs ...
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bioRxiv - Microbiology 2022Quote: ... (rabbit anti-PDI, Cell Signaling, Cat #: 3501, 1: 200 dilution) (rabbit anti-Flag, Cell Signaling, Cat #: 14793, 1:250 dilution) (mouse anti-PDI, Thermo Fisher, Cat #: MA3-019, 1:200 dilution) (mouse anti-Spike S2 ...
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bioRxiv - Immunology 2022Quote: ... SDS-polyacrylamide NuPAGE™ gels Gels were ransferred to a nitrocellulose membrane by the iBlot Gel Transfer System (Invitrogen), membranes blocked for one hour in 5% nonfat milk in 25 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... Protein gels were run using sodium dodecyl sulfate polyacrylamide gradient gels (NuPage 4−12% Bis-Tris gel from Invitrogen) using the BenchMark protein ladder (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... and the resulting PCR product was subjected to gel electrophoresis (E-Gel™ EX Agarose Gels, 2% [ThermoFisher G402002]), and gel extraction (Wizard™ SV Gel and PCR Cleanup System [Promega A9282] ...
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bioRxiv - Cancer Biology 2022Quote: ... Restriction digest products were subjected to gel electrophoresis in 1% agarose gel + 1X SYBR Safe DNA Gel Stain (Invitrogen). DNA bands of digested DNA were excised and purified using NucleoSpin Gel and PCR Clean-Up kit (Machery-Nagel ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were purified by tandem affinity chromatography using Glutathione Agarose resin (Thermo Fisher Scientific) followed by Ni-NTA resin ...
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bioRxiv - Microbiology 2021Quote: ... and purification was conducted using gravity flow over nickel affinity resin (Thermo-Fisher Scientific) as previously described (North et al. ...
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bioRxiv - Biophysics 2020Quote: ... DtpA was purified by Immobilized Metal Affinity Chromatography (IMAC) using Ni-NTA agarose (ThermoFisher). His-tagged proteins were bound to the resin for 60 min at 4°C on a rotating wheel ...
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bioRxiv - Immunology 2021Quote: ... The flowthrough was then purified over CaptureSelect™ IgG-CH1 affinity resin (ThermoFisher Scientific), and bound Fab fragments eluted with 200 mM acetic acid with 50 mM NaCl (pH 3.5 ...
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bioRxiv - Immunology 2021Quote: ... ACPA were affinity isolated from 25µl serum samples using NeutrAvidin Plus resin (Thermo Scientific) coupled with 0.1µg/µl CCP2-biotin followed by IgG capturing using FcXL affinity beads (Thermo Scientific) ...
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Molecular basis of selective cytokine signaling inhibition by antibodies targeting a shared receptorbioRxiv - Immunology 2021Quote: ... proteins were purified by immobilized metal affinity chromatography (IMAC; HisPur NiNTA resin, ThermoFisher Scientific). The His-tag was removed by Tobacco Etch Virus (TEV ...
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bioRxiv - Cell Biology 2021Quote: ... followed by affinity precipitation of biotinylated molecules by streptavidin-conjugated Dyna beads (Thermo Fisher). The eluted samples were subjected to Western Blotting analyses using rabbit monoclonal anti-MT1-MMP antibody.
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bioRxiv - Cell Biology 2021Quote: ... Proteins were purified by tandem affinity chromatography using glutathione agarose resin (Thermo Fisher Scientific) followed by Ni-NTA resin ...
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bioRxiv - Cell Biology 2020Quote: High affinity protein-binding 96-well plates (Nunc™ Maxisorp™ Cat No. 442404) were coated with 1 μg of protein (100 μL of 10 μg/mL of protein solution in PBS ...
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bioRxiv - Biochemistry 2022Quote: ... All proteins were initially purified using HisPur Ni-NTA affinity resin (Thermo Fisher Scientific) using Ni-NTA buffer supplemented with 25 mM imidazole during wash and 250 mM imidazole during elution ...
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bioRxiv - Biophysics 2022Quote: ... 5 μM Fluo-2 Low Affinity (TefLabs) and 2.5 μM Alexa568 (Invitrogen, Waltham MA) as previously described (36) ...
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bioRxiv - Genomics 2019Quote: ... 180 μg number and affinity library vector and 250 μL P3000 (Thermo Fisher Scientific) following the recommended protocol ...
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bioRxiv - Genomics 2019Quote: ... 2.79 μg number and affinity library vector and 8.8 μL P3000 (Thermo Fisher Scientific) following the recommended protocol ...
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bioRxiv - Biochemistry 2019Quote: Binding affinity of guanine nucleotides BODIPY-FL-GTPgammaS or BODIPY-FL-GDP (Molecular Probes) were determined using fluorescence polarization measured by an EnVison 2102 Multilabel Plate Reader (Perkin Elmer).
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bioRxiv - Biochemistry 2021Quote: ... The clear supernatant was incubated with Ni-NTA affinity resin (Thermo Fisher Scientific, USA). PLpro was eluted by gravity flow using lysis buffer supplemented with 300 mM imidazole and subsequently incubated with TEV protease at a molar ratio of 20:1 in the presence of 1 mM DTT ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was loaded onto a Ni affinity column (Ni-Sepharose 6 FF, Invitrogen). The column was washed with the lysis buffer A supplemented with 10 mM imidazole ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were purified by tandem affinity chromatography using glutathione agarose resin (Thermo Fisher Scientific) followed by Ni-NTA resin ...
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bioRxiv - Plant Biology 2022Quote: ... Biotin affinity purification was then performed by using Dynabeads MyOne Streptavidin C1 beads (Invitrogen). Library amplification was done with Ultra II Q5 Master Mix with universal and selected index primers.
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bioRxiv - Microbiology 2023Quote: ... as described above and purified using the CaptureSelect C-tagXL Affinity Matrix (Thermo Scientific). The elution was concentrated and buffer-exchanged into 20 mM HEPES ...
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bioRxiv - Immunology 2023Quote: ... and rS1Gamma were purified using a CaptureSelectTM C-tagXL Affinity Matrix prepacked column (ThermoFisher) and followed the manufacturer’s guidelines ...