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Citations for Thermo Fisher :
551 - 600 of 10000+ citations for Anti Flag Affinity Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and separated on SDS 4 - 12 % Bis-Tri -polyacrylamide gel electrophoresis gels (Invitrogen). Polyclonal rabbit anti-GFP antibodies (ThermoFisher ...
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bioRxiv - Genomics 2022Quote: ... Gel purification of pooled products from a 2% E-gel EX (Life Technologies) were performed using the QiaQuick Gel Extraction kit (Qiagen).
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bioRxiv - Microbiology 2021Quote: ... and loaded onto SDS-page gels (4-12% Bis-Tris Plus gels, ThermoFisher); the proteins were detected by scanning the gel for green (GFP ...
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bioRxiv - Biochemistry 2019Quote: ... Gels were blotted using an iBlot Gel Transfer Device and Transfer Stacks (ThermoFisher). Blots were blocked with 5% milk in PBS for 1 h at room temperature and incubated with primary antibody overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The RNA gel was stained using SYBR Gold Nucleic Acid Gel Stain (Invitrogen). Fluorescence imaging was performed using an Amersham Typhoon imager (GE Healthcare ...
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bioRxiv - Molecular Biology 2020Quote: ... and loaded onto SDS-page gels (4-12% Bis-Tris Plus gels, ThermoFisher); the proteins were detected by scanning the gel for green (GFP ...
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bioRxiv - Cell Biology 2020Quote: ... resolved on 10% polyacrylamide gels or pre-cast gradient gels (Thermo Scientific, NW04120BOX) and transferred onto PVDF membrane (Millipore ...
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bioRxiv - Microbiology 2021Quote: ... the gels were stained with SYBR green nucleic acid gel stain (ThermoFisher Scientific) in 0.5X TBE/10% glycerol for 30 min with gentle agitation and then the fluorescence was imaged with a Typhoon Trio phosphoimager (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... Gel electrophoresis was performed using Bolt 4-12% Bis-Tris gels (Life Technologies) with 40μ of sample loaded per well ...
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bioRxiv - Cancer Biology 2022Quote: ... Gel electrophoresis was performed using NuPage 4-12% Bis-Tris Midi gels (Invitrogen) in NuPage MOPS SDS Running Buffer (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: ... The gel was stained with SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen) before visualization using a Molecular Imager Gel Doc XR System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... Size selection was performed using gel extraction with a 6% TBE gel (Invitrogen). Validation of 22G-RNA libraries was done on a 2200 TapeStation (Agilent).
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bioRxiv - Immunology 2023Quote: ... Lysates were then loaded into NuPAGE gels (4-12% Bis–Tris gels, Invitrogen) and run at 150 V for 90 min ...
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bioRxiv - Synthetic Biology 2023Quote: ... Agarose gels were stained with SYBR Gold Nucleic Acid Gel Stain to (Invitrogen) and PFGE patterns were scanned using the Vilbert Lourmat E-BOX VX2 Complete Imaging system.
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bioRxiv - Microbiology 2023Quote: ... Sequencing libraries were purified by gel electrophoresis on 8% TBE gels (Invitrogen Novex), stained with SYBR Gold (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... The PCR product sizes were verified on agarose gel (E-Gel EX; Invitrogen) and quantified with fluorometry (Qubit ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were size-selected with E-Gel EX 2% agarose gels (Life Technologies) and purified by QIAquick Gel Extraction Kit (QIAGEN) ...
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bioRxiv - Plant Biology 2023Quote: ... The amplicons were gel purified using GeneJet Gel Extraction Kit (Thermo Fisher Scientific) and subjected to Sanger sequencing followed by in silico analysis using SnapGene v6.2 software (GSL Biotech LLC).
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bioRxiv - Biophysics 2023Quote: Gels were blotted for 7 min using an iBlot Gel Transfer Device (Invitrogen). All further incubation steps were performed under agitation at room temperature if not stated otherwise ...
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bioRxiv - Molecular Biology 2023Quote: ... Size selection was performed using gel extraction with a 6% TBE gel (Invitrogen). Validation of 22G-RNA libraries was done on a 2200 TapeStation (Agilent).
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bioRxiv - Molecular Biology 2023Quote: ... Gel electrophoresis was performed using NuPAGE 4-12% Bis-Tris precast gels (Invitrogen) and MOPS-SDS running buffer (Boston Bioproducts) ...
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bioRxiv - Genomics 2022Quote: ... DNA quality was checked on a 1% agarose gel (E-gel EX Invitrogen). 500ng DNA was used from each sample for further analysis ...
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bioRxiv - Biochemistry 2023Quote: ... The gel was stained with SYBR Safe DNA gel stain (Thermo Fisher Scientific) to visualize DNA bands ...
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bioRxiv - Biochemistry 2023Quote: Native gel electrophoresis was performed using 3-12% Bis-Tris gels from Invitrogen and native running buffers from SERVA.
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bioRxiv - Synthetic Biology 2023Quote: ... Gel-electrophoresis was performed in MES buffer and commercially available polyacrylamide gel (ThermoFisher). The transfer of proteins to the PVDF Transfer Membrane (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: Gel electrophoresis was performed using 4-12% NuPAGE Bis-Tris gels (Thermo Scientific). Proteins were transferred to 0.45-μm nitrocellulose membranes for 60 min at 35 V ...
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bioRxiv - Molecular Biology 2024Quote: ... Barcoded libraries were gel-purified using the PureLink Quick Gel Extract kit (ThermoFisher) and sequenced on an Illumina HiSeq2500.
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bioRxiv - Molecular Biology 2023Quote: ... the gels were stained with SYBR Gold nucleic acid gel stain (Invitrogen #S11494). Imaging was performed with a Chemidoc MP (Bio-Rad Laboratories).
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bioRxiv - Neuroscience 2024Quote: ... The amplified DNA was resolved on E-Gel 2% agarose gel (Invitrogen, G401002) on E-Gel Power Snap Electrophoresis device (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... wedge-well polyacrylamide gels and were electrophoresed in a Mini Gel Tank (Invitrogen) using MES buffer at 200V for 25 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... gel purified using GeneJET Gel Extraction and DNA Cleanup Micro Kit (Thermo Scientific), and then electroporated into a JM217 strain containing pSIM5 55 that encodes λ-Red proteins ...
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bioRxiv - Bioengineering 2019Quote: ... Collected samples were analyzed using native gel electrophoresis (NativePAGE 4-16% Bis-Tris Gel) or denature gel electrophoresis (SDS-PAGE, Novex 4-20% Tris-Glycine Mini Gels, Thermo Fisher Scientific). For SDS-PAGE ...
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bioRxiv - Systems Biology 2020Quote: ... and custom-designed primers with barcodes.30 Gel electrophoresis was used to remove primer dimers from the PCR products with 2% E-Gel EX Agarose Gels (Thermo Fisher Scientific), and then the PCR products were purified using QIAquick PCR Purification Kit (Qiagen) ...
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bioRxiv - Microbiology 2021Quote: ... The digested product was then electrophoresed on 1% (w/v) agarose gel and purified from the gel slice using PureLink Quick Gel Extraction Kit (Thermo Fisher Scientific). The purified product was subcloned into pACYC177 plasmid at DraIII-XhoI sites ...
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bioRxiv - Plant Biology 2022Quote: ... The specificity of the primers was further validated using PCR and DNA gel electrophoresis on 1% (w/v) agarose gels containing 1X SYBR Green I Nucleic Acid Gel Stain (Thermo Fisher Scientific) in 1X TAE buffer ...
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bioRxiv - Immunology 2022Quote: ... The PCR product was visualized through TAE agarose gel electrophoresis and gel purified with Purelink Quick Gel Extraction kit (Thermo Fisher Scientific) per manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... The amplification and quality of the ChIPseq libraries were checked by running 10% of the samples in E-Gel Agarose Gels with SYBR Safe DNA Gel Stain (ThermoFisher Scientific, USA), and if necessary ...
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bioRxiv - Bioengineering 2020Quote: ... were loaded and resolved on a two-layer SDS-PAGE gel (12% resolving gel; 4% loading gel) together with pre-stained standards (ThermoFisher Scientific, UK). Separated proteins were electro-transferred to PVDF membranes (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... An aliquot of the 25 µL reaction was run on a 1% ethidium bromide agarose gel (or 1% E-Gel agarose gel, Thermo Fisher Scientific) to verify 1 unique PCR product band ...
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bioRxiv - Microbiology 2023Quote: ... Both reactions provided two products per reaction that were gel purified in a 2% agarose gel using a GeneJet Gel Extraction kit (Thermo Scientific corp.). Each product was cloned using the pGEM®-T Easy vector kit (Promega Corp. ...
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bioRxiv - Cell Biology 2023Quote: ... Protein material was resolved by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) in 12-well gels (Invitrogen, 4-12% Bis-tris BOLT gels) in 0.9x MOPS-SDS buffer (Invitrogen) ...
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bioRxiv - Molecular Biology 2019Quote: ... HCT116 Flag-Cas9 cells were cultured in McCoy’s 5A medium (Gibco) supplemented with 10% FBS and Pen/Strep ...
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bioRxiv - Molecular Biology 2019Quote: ... FLAG (M2) antibody was coupled to protein G dynabeads (Thermo Scientific). The RNA-protein complexes were immunoprecipitated over night at 4°C ...
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bioRxiv - Biochemistry 2019Quote: ... MCM3 was cloned with an N-terminal FLAG tag (DYKDDDDK, ThermoFisher). The coding sequence for MCM2 was cloned into QB3 Macrolab vector 4C to generate an N-terminal His6-MBP fusion ...
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bioRxiv - Biochemistry 2021Quote: ... The pcDNA-FLAG plasmid was purchased from Thermo Fisher (ThermoFisher Scientific). pcDNA3-FLAG-RIPK3 (Addgene plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... and antibodies and normalized to gp130-Flag (cat # 125623, Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... for FLAG® (DYKDDDDK)-tagged proteins in iBind Western System (Invitrogen). Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... and the upper half using 1:1000 diluted α-FLAG (ThermoFisher) to detect FANCD2 ...
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bioRxiv - Molecular Biology 2019Quote: ... contained 40 µM human Flag-tagged ubiquitin (Fisher Scientific U-120), 0.5 µM human His6-tagged Ubiquitin E1 enzyme (Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and FLAG-CUL4B and mutants in pcDNA5/FRT/TO vector (Invitrogen).