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Citations for Thermo Fisher :
3901 - 3950 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... The supernatant containing the virus was then used fresh or stored by snap-freezing aliquots supplemented with 8 μg/ml polybrene (Fisher Scientific) in ethanol/dry ice and stored at -80° C ...
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bioRxiv - Cell Biology 2022Quote: ... Transduction efficiency was assessed by GFP expressing virus and subsequent quantification of GFP-positive cells by Countess II FL Cell Counter (Thermo Fisher).
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from 50 μl of virus inoculum suspension using the PureLink Viral RNA/DNA Mini kit (Themo Fisher Scientific), as per manufacturer’s directions.
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bioRxiv - Microbiology 2022Quote: ... and resuspended in thawed Geltrex lactose dehydrogenase elevating virus free (LDEV-free) reduced growth factor basement membrane matrix (Thermo Fisher Scientific). Three 10-μl drops of the matrix-cell suspension were pipetted into wells of a Nunclon Delta 24-well plate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: Copies of the SARS-CoV-2 N gene (genomic) were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... The virus-containing samples (50 μl) were serially diluted in 450 μl aliquots of serum-free Opti-Pro medium (Gibco, UK), after which 200 μl from each dilution were added in duplicates to the cells ...
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bioRxiv - Neuroscience 2022Quote: ... HT-1080 cells (ATCC CCL-121; RRID:CVCL_0317, male) used for virus titration were grown and maintained in MEM (Gibco Cat#11095-080) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... The samples were used for quantitating HuNoV RNA GEs by means of reverse transcription-quantitative PCR (RT-qPCR) analysis using a TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and GII specific primer/probe sets ...
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bioRxiv - Cancer Biology 2024Quote: ... Subsequent qPCR was performed using the TaqMan Fast Virus 1-Step Master Mix and StepOnePlus Real-Time PCR System (Thermo Fisher). The primers purchased from TaqMan were ...
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bioRxiv - Neuroscience 2024Quote: ... HEK cells below passage 10 are plated into a 15 cm dish/virus (Fisher 08-772-24) in DMEM-H (DMEM-h (invitrogen 10569010) + NEAA (Invitrogen 11140050) + 10% FBS (invitrogen 16140071 ...
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bioRxiv - Genetics 2024Quote: The derivation of iPSCs was carried out by the NHLBI iPSCs core facility using the Cytotune 2.0 Sendai virus kit (ThermoFisher Scientific, A16517) with a few modifications for high throughput reprogramming essentially as described earlier (19) ...
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bioRxiv - Microbiology 2024Quote: ... Virus stocks were grown in M2-10B4 cells (ATCC # CRL-1972) with RPMI 1640 Medium special formulation (Thermo Fisher cat # A1049101) supplemented with 10% fetal bovine serum (Thermo Fisher cat # 12483020 ...
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bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Anesthetized mice were inoculated intranasally with 30 μL of virus diluted in chilled 1X PBS (MHV) or administered undiluted in 2% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Both assays were performed using the Taqman Fast Virus 1-Step Master Mix RT-qPCR kit (Applied Biosystems Inc., Carlsbad, CA) on an LC96 instrument (Roche ...
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bioRxiv - Cell Biology 2023Quote: The adeno-associated virus MyoAAV.2A was produced using the AAV-MAX Helper-Free AAV Production System Kit (Thermo Fisher Scientific). At 72 h post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were infected (multiplicity of infection of 6 for each virus) with separate baculoviruses (prepared using the Bac-to-Bac Baculovirus Expression System, Invitrogen) containing the CXCL12LRHQ gene as well as the ACKR3 gene (residues 2-362 ...
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bioRxiv - Immunology 2023Quote: ... 2μg liver RNA per sample was used for qRT-PCR analysis for Plasmodium 18S rRNA using TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). Data were normalized for input to the GAPDH control for each sample and are presented as ratios of Plasmodium 18s rRNA to GAPDH RNA signals ...
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bioRxiv - Microbiology 2023Quote: We diluted freshly purified virus samples in DPBS and combined three volumes of the virus with one volume of NuPAGE LDS Sample Buffer (4×; Thermo Fisher) containing 100 mM dithiothreitol ...
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bioRxiv - Bioengineering 2023Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Microbiology 2023Quote: ... For intravenous inoculation mice were restrained and injected via tail vein injection with 100 to 200 μl of virus or control inoculum using a 29G insulin syringe (Fisher Scientific). For natural transmission ...
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bioRxiv - Pathology 2023Quote: ... was reverse transcribed at 37°C for 50 min in a 20 µl reaction mix containing 200U Moloney murine leukemia virus (MMLV) reverse transcriptase (Life Technologies), 100 ng random primers ...
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bioRxiv - Genomics 2023Quote: ... the fibroblasts were thawed and plated at a density of 250K cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Microbiology 2023Quote: ... Viral genome levels were quantified by qPCR using virus specific primer probe sets (described above) and normalised to GAPDH using an Applied Biosystems FAM/MGB probe mix (Thermofisher, 4333764F).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Patients consent was retrieved and the iPSCs were generated from peripheral blood mononuclear cells (PBMCs) using Sendai virus (CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit, Thermo Fisher Scientific) as previously described ...
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bioRxiv - Neuroscience 2024Quote: ... The MS19-ES-H line was reprogrammed from PBMCs from a healthy female donor with a Cytotune Sendai Virus kit (Thermo Fisher).
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bioRxiv - Microbiology 2024Quote: ... isolated RNA was run using a TaqMan Fast Virus One-Step Master Mix (applied Biosystems) on a QuantStudio 3 Flex Real-Time PCR system (Applied Biosystems). RNA standards with known copy numbers were used to generate a standard curve and calculate sample copy numbers ...
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bioRxiv - Cancer Biology 2024Quote: ... Virus to construct stable HA-B56α overexpression cell lines were produced using HEK293T cells transfected using Lipofectamine 3000 (Fisher Scientific, L3000015) with the plasmid of interest (POI ...
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bioRxiv - Neuroscience 2024Quote: ... The fluorescent protein expressed by the virus (either GFP or mCherry) was visualized using an EVOS FL tabletop fluorescent microscope (ThermoFisher Scientific). Images were taken for future reference ...
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bioRxiv - Biophysics 2021Quote: ... B16F10 cells were cultured in DMEM medium (containing with 4.5 g/L glucose, L-glutamine, and sodium pyruvate) supplemented with 10% FBS (Life technologies, CA, USA), and 100 IU/mg penicillin-streptomycin (Life technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed with PBS and incubated with corresponding secondary antibodies goat anti-rabbit IgG Alexa Fluor 555 (H+L) and goat anti-mouse IgG (H+L) Alexa Fluor 647(all Thermo Fisher Scientific) for 1 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... The ablated tissue was transported by helium gas (0.9 L/min) with make-up argon gas (0.7 L/min) to a quadrupole ICP-MS device (iCAP TQ, ThermoFisher Scientific, Bremen, Germany), measuring 56Fe and 13C ...
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bioRxiv - Molecular Biology 2020Quote: ... Alexa Fluor 488-conjugated goat anti-rat IgG (H + L) antibodies and Alexa Fluor 594-conjugated goat anti-mouse IgG (H + L) antibodies (ThermoFisher Scientific, A11005) were used as secondary antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... Yeast cells were cultured overnight in 2 mL of YPAD liquid medium (10 g/L Bacto Yeast Extract, #212750, Thermo Fisher Scientific, Waltham, MA, USA; 20 g/L Bacto Peptone, #211677, Thermo Fisher Scientific;100 mg/L adenine sulfate ...
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bioRxiv - Molecular Biology 2019Quote: ... The next day, media was replaced by Heavy Lysine (36C6, 15N2 L-Lysine-2HCl), Arginine (13C6, 15N4 L-Arginine-HCl) DMEM (Thermo Fisher Scientific) with 10% dialyzed FBS containing 1ug/ml doxycycline and grown for either 1 day or 2 days ...
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bioRxiv - Genomics 2020Quote: ... We extracted DNA with 0.9 mL Extraction Buffer (1% SDS [Fisher Scientific], 240 mg/L para-aminosalicyclic acid [ACROS], 484 mg/L Tris/HCl [Fisher Scientific] ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were subsequently incubated in blocking buffer containing HRP-conjugated anti-mouse IgG (H+L) (1:2000, Fisher 62-6520) or HRP-conjugated anti-rabbit IgG (H+L) (1:2000, Life Technologies 31462) for one hour ...
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bioRxiv - Neuroscience 2022Quote: Lumbar DRG (L2-L5) were collected post-mortem and placed into cold dissociation media (L-15 Medium (1×) + GlutaMAX-l (Life Technologies, UK) supplemented with 24 mM NaHCO3) ...
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bioRxiv - Molecular Biology 2022Quote: For all proteomic analysis, HMI-9 SILAC medium (Urbaniak, Martin, and Ferguson 2013)-L-Arginine and – L-Lysine (Gibco, reference 074-91211A) was used ...
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bioRxiv - Biochemistry 2021Quote: ... N2-rich water (3.50 mg/L) and normal water without treatment (Air) (8.75 mg/L) was determined using an oxygen electrode (Thermo Fisher Scientific, USA).
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bioRxiv - Biochemistry 2019Quote: ... The mixture was alkylated using 10 µL of 400 mmol/L 2-chloroacetamide (CAA, Thermo Scientific; final concentration of 40 mmol/L) and incubated in the dark at room temperature for 30 min ...
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bioRxiv - Immunology 2020Quote: ... secondary mAbs (anti-mouse and anti-rabbit IgG H&L, Alexa Fluor® 488 - Abcam; anti-rabbit IgG H&L, Alexa Fluor® 647 - Invitrogen) was added in 0.5% BSA in PBS for 1 h at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... After further incubation with horseradish peroxidase-conjugated goat anti-mouse IgG H&L and goat anti-rabbit IgG H&L (Thermo Fisher Scientific) for 1 hour ...
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bioRxiv - Biochemistry 2019Quote: ... The mixture was alkylated using 3.75 µL of 375 mmol/L iodoacetamide (IAA, Pierce, Thermo Scientific; final concentration of 15 mmol/L) and incubated in the dark at room temperature for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... was washed twice with ChIP RIPA buffer and blocked with 1 g/l BSA and 0.4 g/l sonicated salmon sperm DNA (Life Technologies no. 15632011) overnight ...
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bioRxiv - Biochemistry 2020Quote: ... L-023324-00-0005) or UBE2D2 (Cat. No. L-010383-00) (Dharmacon) were introduced into cells using Lipofectamine RNAiMAX (Thermo Fisher Scientific.), as per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... Transformed bacteria were directly inoculated into 2 L plastic baffled flasks (Thomson Instrument Company) containing 200 mL optimised growth medium with 15 g/L tryptone (Thermo Fisher Scientific), 30 g/L yeast extract (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... or regular amino acids (0.1 mg/ml L-lysine-HCl and 0.1 mg/ml L-arginine-HCl [both FUJIFILM Wako Chemicals]) were added to DMEM deficient in both L-lysine and L-arginine for SILAC (Thermo Fisher Scientific) supplemented with 10% dialyzed fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2022Quote: ... One plate was cultured in light SILAC media: DMEM deficient in L-lysine and L-arginine (DMEM for SILAC, Thermo Fisher, A33822) supplemented with 84 mg/ml L-arginine (Arg0 ...