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Citations for Thermo Fisher :
3751 - 3800 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
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bioRxiv - Cancer Biology 2022Quote: ... All cell lines were validated free of virus mycoplasma contamination using the MycoSEQ assay (Thermo Fisher Scientific, Waltham, MA, USA) or STAT-Myco assay (IDEXX Bioanalytics) ...
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bioRxiv - Microbiology 2022Quote: Blood virus mixtures were titrated by ten-fold serial dilution in virus media (RPMI 1640 cell culture media supplemented with 5% fetal bovine serum [FBS] and 1% Penicillin-Streptomycin [Gibco]) in a 96-well plate before transferring dilutions to equivalent wells of a 96 well plate containing near confluent C6/36 cell monolayers ...
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bioRxiv - Neuroscience 2022Quote: Total RNA isolation from the AAV9 virus injected region of the left frontal cortex was performed as previously described [40] by TRIzol LS Reagent (10296010, Invitrogen). For cDNA synthesis ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Virus propagation and titration was performed in LLC-MK2 cells using Opti-MEM supplemented with 1.6% TrypLE™ Express (Gibco) and 100 μg/ml CaCl2 (OptiMEMinf ...
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bioRxiv - Plant Biology 2022Quote: ... was transcribed following DNase I treatment using Moloney Murine Leukemia Virus reverse transcriptase and an oligo dT(18) primer according to the manufacturer’s protocol (ThermoFisher Scientific). qPCR analysis was carried out in a CFX96-C1000 96-well plate thermocycler (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... 5% CO2 until the following day. Virus stocks were diluted into supplemented media (DMEM from Gibco, Cat. no. 61965026 supplemented with 2% FBS (Gibco, Cat ...
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bioRxiv - Neuroscience 2022Quote: ... 1 µg of total RNA was subjected to random reverse transcription using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV-RT; Invitrogen) and random primers (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... PBMCs were reprogrammed into iPS cells using transient overexpression of pluripotency-associated factors (OCT4, KLF4, and SOX2) via non-integrating Sendai virus particles (Cytotune 2.0 kit, Life Technologies). At least five clones per TSC2+/+ and TSC2+/− donor were established into iPS cell lines ...
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bioRxiv - Genetics 2024Quote: ... The iPSCs were made Sendai virus free by keeping in culture and testing for the presence of Sendai Virus (SeV) at different times by measuring SeV levels in total RNA using RT-qPCR with FAM labeled SeV (Thermofisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... cDNA of the virus genome was generated by reverse transcription with random hexamer as the primer (SuperScript III, ThermoFisher Scientific). The HA1 subunit was divided into three fragments for PCR (PrimeSTAR Max DNA Polymerase ...
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bioRxiv - Synthetic Biology 2023Quote: ... The virus concentration using Lenti-X Concentrator was performed with the manufacturer’s protocol and dissolved in Opti-MEM (Gibco #31985062) to yield a 10- or 15-fold concentration ...
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bioRxiv - Microbiology 2023Quote: ... generated by Knut Falk in the late 1990s) or mouse IgG2a targeting influenza virus NP (clone D67J, Thermo Fisher Scientific) (both ...
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bioRxiv - Developmental Biology 2023Quote: ... PD2 and TD1 hiPSC were generated from deidentified commercially available human peripheral blood mononuclear cell and tracheal epithelial cell lines via the manufacturing protocol of Sendai virus-mediated reprogramming (CytoTune2.0) (ThermoFisher, A16517). Every other month ...
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bioRxiv - Microbiology 2023Quote: BmN cells infected with WT virus at indicated time were harvested and total RNA was extracted by using TRIzol reagent (Invitrogen). 2 μg total RNA was prepared to synthesis the cDNA by TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (TransGen) ...
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bioRxiv - Microbiology 2023Quote: ... The following day we washed cells with DPBS twice and added half-log10 dilutions of the virus sample in infection media (MEM medium (Gibco) containing 0.3% BSA (fraction V ...
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bioRxiv - Microbiology 2023Quote: ... were transfected with each plasmid expressing SPΔC of SARS-CoV-2 Delta variant (1.2 µg DNA) or human influenza A virus M2 (5 µg DNA) using Lipofectamine 3000 (Invitrogen, USA) to produce 293TN-S target cells ...
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bioRxiv - Microbiology 2022Quote: ... The RT-qPCR was performed according to the manufacturer’s protocol using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher). Fluorescent signals were acquired using QuantStudio 6 Real-time PCR system (Thermo Fisher).
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bioRxiv - Microbiology 2023Quote: ... Lungs from K18-ACE2 C57BL/6 mice infected with virus were collected at indicated time points and were homogenized in Trizol (Invitrogen) and RNA was isolated using manufacturer’s instructions ...
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bioRxiv - Pathology 2023Quote: ... Total RNA was extracted from clinical specimens (swabs and tissues) and virus isolates using the MagMax AM1836 96 Viral RNA Isolation Kit (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: ... A total of 1 µg of RNA was used as a template for cDNA synthesis using Moloney Murine Leukemia Virus reverse transcriptase (M-MLV RT) (Invitrogen).
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bioRxiv - Systems Biology 2024Quote: ... 1 million 293T STING-mNG or 0.5 million BJ1 seeded the same day in one well of a 6 well plate were transduced with 2ml of virus including 8µg/ml Protamine (Thermo Fisher). Cells were incubated with viral supernatant for 1-2 days and cells were then transferred in a fresh flask for selection ...
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bioRxiv - Biochemistry 2023Quote: ... Concentrated virus was applied through a 0.45 μm surfactant free cellulose acetate syringe filter (Nalgene, Fisher Scientific #09-740-106) and immediately used or stored at -80ºC for up to one month ...
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bioRxiv - Plant Biology 2023Quote: ... RT-PCR was performed with 1 µg of RNA using 100 units of Moloney murine leukemia virus reverse transcriptase (Invitrogen) according to instruction of the manufacturer ...
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bioRxiv - Genomics 2024Quote: ... the fibroblasts were thawed and plated at a density of 2.5×105 cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Cell Biology 2024Quote: ... in this study were generated from deidentified commercially available human tracheal epithelial cell lines via the manufacturing protocol of Sendai virus-mediated reprogramming (CytoTune2.0) (Thermo Fisher) as described before24 ...
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bioRxiv - Cell Biology 2024Quote: We established mouse iPSC lines (CD1 iPSC or ntdTomato iPSC) using Sendai virus-mediated reprogramming kit: CytoTune2.0 (Thermo Fisher, A16517) and followed manufactured protocol as described before24 ...
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bioRxiv - Neuroscience 2024Quote: ... sections of virus-injected mice were incubated in a solution of chicken anti-eGFP (1:2000, Thermo Fisher Scientific, A10262) and mouse anti-gephyrin (1:100 ...
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bioRxiv - Developmental Biology 2024Quote: ... The 10µl of cells suspension infected with virus was plate micromass cultures (3-4 spots per 35mm culture dish, NUNC #150318) at a density of 2 x 107 cells/ml ...
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bioRxiv - Biochemistry 2024Quote: ... Ten micrograms of total RNA was reverse transcribed (RT) at 37 °C using random hexamer primers and Moloney murine leukemia virus retrotranscriptase (Invitrogen-Life Technologies ...
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bioRxiv - Microbiology 2024Quote: Virus-infected cells and the supernatants were dissolved with Tris-glycine SDS sample buffer (Thermo Fisher Scientific, Waltham, MA, USA), boiled for 5 min in the absence of reducing agent ...
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bioRxiv - Cell Biology 2024Quote: ... were co-transfected with vesicular stomatitis virus G (600 ng) and pSPAX2 (800 ng) using Lipofectamine 3000 (Cat# L3000001, ThermoFisher). The lentiviral supernatants were collected 2 days after transfection ...
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bioRxiv - Cell Biology 2020Quote: ... then incubated with the Alexa Fluor 488-conjugated goat anti-mouse IgG (H + L) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) secondary antibodies (1:300, Molecular Probes) for 1 h at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... All DLBCL cell lines were grown in RPMI-1640 with 25 mM HEPES and 2 mM L-alanine-L-glutamine (GlutaMAX) (Gibco 72400), supplemented to 4.5 g/L D-glucose ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-Mouse IgG (H+L) and goat anti-Rabbit IgG (H+L) conjugated to horseradish peroxidase (1:10,000, Thermo Fisher Scientific). Loading control was obtained incubating with anti-actin-horseradish peroxidase conjugated (1:100,000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... were directly added to yield 50 μL PURE system reaction mixtures [28] containing 13C6 15N4 L-Arginine (R*) and 13C6 L-Lysine (K*) (Thermo Scientific) as a substitute for non-labeled L-Arginine and L-Lysine ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-rabbit Alexa Fluor 488 IgG H+L (1:400, A11008) and goat anti-mouse Alexa Fluor 568 IgG H+L (1:250, A11004) (Invitrogen, ThermoFisher).
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor®(568) conjugated donkey anti-rabbit IgG(H+L), Alexa Fluor®(568, 647) conjugated donkey anti-mouse IgG(H+L) (ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: ... and cultured in minimal medium containing 1 part of DMEM (4.5 g/L D-Glucose, Pyruvate, L-Glutamine; Life Technologies; 41966029): 1 part DMEM/F12 (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... A volume of 18 µL of the master mix was added to each well of a 96-well PCR plate (AB-0600-L, Thermo Scientific) with 2 µL of the previously synthesized 1 µg/µL cDNA ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-rabbit IgG (H+L) highly cross-adsorbed AlexaFluor A488 for NTSR2 and goat anti-chicken IgY (H+L) AlexaFluor A594 (Life Technologies) for MAP2 diluted in blocking solution at 1:800 ...
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bioRxiv - Systems Biology 2020Quote: ... SILAC DMEM medium (Thermo #88364) lacking L-arginine and L-lysine was firstly supplemented with 10% dialyzed FBS (Thermo Fisher, # 26400044) and the same penicillin/streptomycin mix ...
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bioRxiv - Neuroscience 2020Quote: ... were collected from FB labelled mice 7-10 days after knee injections in ice cold dissociation media containing L-15 Medium (1X) + GlutaMAX-l (Life Technologies), supplemented with 24 mM NaHCO3 ...
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bioRxiv - Synthetic Biology 2021Quote: Template DNA in 10 μ L of the TXTL reaction was degraded by adding 0.5 μ L of TURBO DNase (2U/μ L, Catalog No. AM2238, Invitrogen). The mixture was incubated at 37°C for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... OCR and ECAR were measured in un-buffered DMEM supplied with 4.5g/L D-glucose and 10mM L-glutamine (Gibco, MA, USA). OCR and ECAR values were normalized to cell numbers ...
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bioRxiv - Genomics 2021Quote: ... Then 100 μ L of this solution was gently mixed with 8 μ L of Lipofectamine™ 2000 (lipo) (Invitrogen, USA). A solution of lipo and siRNA (total volume 200 μ L ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 488-conjugated goat anti-mouse IgG (H + L) and Alexa Fluor 594-conjugated goat anti-rabbit IgG (H + L) secondary antibodies were obtained from Molecular Probes.
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bioRxiv - Neuroscience 2022Quote: ... AffiniPure goat anti-mouse IgG(H+L) (JacksonImmuno Research Laboratories Inc.; 115-025-003) and AlexaFluorTM647-R-phycoerythtin goat anti-rabbit IgG(H+L) (A20991,Thermo Fisher)) ...
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bioRxiv - Immunology 2024Quote: Fully differentiated macrophages were transfected with siEPAS1 (L-004814-00-0005, Dharmacon) or siSREBF2 (L-009549-00-0005, Dharmacon) at a final concentration of 5nmol/L using Lipofectamine (RNAiMAx, Life Technologies).
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bioRxiv - Pathology 2024Quote: ... AlexaFluor 488 goat anti-mouse IgG (H+L) and Alexa Fluor 594 goat anti-mouse IgG (H+L) secondary antibodies were used at 1:500 dilution (ThermoFisher Scientific). DAPI (4’,6-diamidino-2-phenylindole ...