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Citations for Thermo Fisher :
3851 - 3900 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: For protein expression and purification experiments we employed the commonly used protein expression strains BL21(DE3) (ThermoFisher Scientific) 20 ...
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bioRxiv - Biochemistry 2024Quote: ... The fluorescence intensity of 100,000 cells per strain was measured using the Attune™ Flow Cytometer (Thermo Fisher). Each strain was measured in biological triplicates ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were induced to produce mov10-YFP by supplementation of the medium with 1 µg/ml doxycycline and then infected with sindbis virus (SINV-mCherry, see below [33]) and then incubated with MitoTracker Deep Red (Thermo Fisher) at 125 nM for 30 minutes before being plunge frozen as above for imaging.
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bioRxiv - Molecular Biology 2021Quote: ... and puromycin resistance marker-Bovine Growth Harmone polyA-signal (amplified from pMK232 (Natsume et al., 2016)) or blasticidin resistance marker-Simian Virus 40 polyA-signal (amplified from pcDNA6/TR (Thermo Fisher)) were cloned into pTwist-Amp (Twist Bioscience ...
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bioRxiv - Molecular Biology 2021Quote: ... was performed using double nucleic acid detection kit for respiratory syncytial virus (A/B) (Shenzhen shengkeyuan) with the ABI Q7 (Applied Biosystems). The reaction was conducted according to the manufacturer’s instructions with total volume of 20 μL.
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bioRxiv - Immunology 2022Quote: ... Two micrograms of liver RNA per sample was used for qRT-PCR analysis for Plasmodium 18S rRNA using TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). Data were normalized for input to the GAPDH control (hepatocytes ...
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bioRxiv - Immunology 2021Quote: ... The isolated RNA was then evaluated in RT-qPCR using the TaqMan Fast Virus 1-step Master Mix (Thermo Fisher Scientific) on a QuantStudio Flex 6 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Bioengineering 2019Quote: iPSCs were reprogrammed from peripheral blood mononuclear cells using the Sendai virus approach (CytoTune-iPS 2.0 Sendai Reprogramming Kit - Thermo Fisher Scientific) according to the manufacturer instruction ...
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bioRxiv - Microbiology 2021Quote: ... and infected at a MOI of 1 with the VSV-ΔG-Fluc-2A-GFP/VSV-G virus diluted in 10mL Opti-MEM (Life Technologies). The cells were incubated 1 hour at 37C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ZIKV RNA samples were each measured in triplicate on an Applied Biosystems ViiA 7 machine using a Taqman Fast Virus 1-Step MasterMix (Thermo Fisher) with primers ZIKV 1087 forward (CCGCTGCCCAACACAAG) ...
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bioRxiv - Microbiology 2021Quote: ... Equal amounts of the cell lysate protein and equal volume of the virus pellet protein were resolved by SDS-PAGE (NuPAGE 4-12% Bis-Tris gel; Invitrogen/Thermo Scientific) and transferred onto nitrocellulose membrane by semidry transfer ...
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bioRxiv - Microbiology 2020Quote: ... 24 h later the dish was washed with PBS and treated with PR8 virus (MOI =2) in the infection medium [F-12K with 2% BSA fraction V (Thermo Fisher), 1% antibiotic-antimycotic (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... 1.5 ml microcentrifuge polypropylene tubes containing the virus (500 µL total volume) were exposed to direct heat in a heat block (Fisher Scientific). After heating ...
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bioRxiv - Immunology 2021Quote: ... The mouse organ tissues were homogenized to detect the virus gene copy number by qPCR (TaqMan Universal Master Mix II with UNG, 4440044, Applied Biosystems). The sequences of the primers are listed in Table 2.
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bioRxiv - Microbiology 2021Quote: ... were seeded in 96-well plates 48h prior to virus inoculation at a density of 5×104 in DMEM (Gibco #41965039) supplemented as described above ...
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bioRxiv - Cancer Biology 2021Quote: ... or empty pLKO.1-puro lentiviral vector were transfected together with pCMV-dR8.9 and vesicular stomatitis virus G–expressing plasmids into 293T cells using Lipofectamine 2000 (Thermo Fisher Scientific) for lentiviral supernatant production as previously described (48) ...
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bioRxiv - Neuroscience 2022Quote: LC-MS/MS analyses were conducted using either a QExactive Plus Orbitrap (QE, RNase-digested polysomes) or a Velos Pro Elite Orbitrap (Elite, virus polysomes and HEK293 aggregates) mass spectrometer (Thermo Fisher) coupled online to a nanoAcquity UPLC system (Waters Corporation ...
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bioRxiv - Cell Biology 2022Quote: ... the unbound virus was removed by washing with infection media followed by agarose overlay with 1X MEM Temin’s modification (Thermo Fisher, 11935046) +0.3% agarose ...
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bioRxiv - Microbiology 2022Quote: Viral RNA in apical secretions was quantified using the TaqMan™ Fast-Virus-1 Step master mix (Thermo Fisher, Basel, Switzerland) with these cycling parameters ...
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bioRxiv - Microbiology 2022Quote: ... HuNoV RNA genome equivalents (GEs) were determined by reverse transcription-quantitative PCR (RT-qPCR) analysis using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and GII specific primer/probe sets (Kageyama et al. ...
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bioRxiv - Bioengineering 2022Quote: ... infected cells were counted and determined by evaluating GFP expression after 24 hours exposure to virus-antibody mixture using Attune NxT Acoustic Focusing Cytometer (Thermo Fisher). Half-maximal inhibitory concentration (IC50 ...
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bioRxiv - Microbiology 2022Quote: ... The levels of RNA corresponding to the N protein-encoding gene of SARS-CoV-2 were measured using the TaqMan Fast Virus 1-step Master Mix (Thermo Scientific). Each 20-μL reaction mixture contained 5.0 μL of 4× TaqMan Fast Virus 1-Step Master Mix ...
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bioRxiv - Plant Biology 2021Quote: ... and then incorporated into the destination vector pMV3 (driven by the cauliflower mosaic virus (CaMV) 35S promoter) using the LR recombination reaction (Invitrogen, USA). All the recombinant constructs were transformed into the Agrobacterium tumefaciens strain C58 by electroporation and subsequently transformed into the tomato genome via explants of cotyledon ...
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bioRxiv - Developmental Biology 2021Quote: ... The virus was spun at 500 g for 5 min and the supernatant filtered through a 45 μm polyethersulfone filter (Fisher Scientific) and subsequently concentrated ...
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bioRxiv - Microbiology 2021Quote: ... viral stocks were prepared after two serial passages of isolated virus in Vero E6 cells in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) supplemented with 5% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Sf9 transfection with bacmid DNA and virus amplification were performed essentially as described for the Bac-to-Bac Baculovirus Expression System (ThermoFisher Scientific). Sf9 cells were maintained in Hyclone CCM3 (CCM3 liquid medium with L-glutamine ...
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bioRxiv - Immunology 2019Quote: ... and cDNA was reverse transcribed using the Moloney murine leukemia virus reverse transcriptase and random hexamer oligonucleotides for priming (Life Technologies). The expression of CPT1 was determined using PCR SYBR Green sequence detection system (Eurogentec ...
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bioRxiv - Systems Biology 2022Quote: All induced pluripotent stem cell (iPSC) lines were reprogrammed by the Sendai virus expressing 4 Yamanaka factors (CytoTune®-iPS Sendai Reprogramming Kit, Invitrogen) as previously described36 ...
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bioRxiv - Cancer Biology 2022Quote: ... except that some preparations were made using donor T cells that were activated for 48 hours before virus infection using LentiBoost (5 μl each of solutions A and B per ml of cells transfected; Fisher Scientific). 100 units/ml IL-2 was added at the start of the activation procedure and with every media change during the expansion phase.
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bioRxiv - Neuroscience 2019Quote: cDNA sequences from the leader through the first half of the rabies virus P gene were amplified using Platinum SuperFi Green Master Mix (Invitrogen (Thermo Fisher), USA ...
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bioRxiv - Microbiology 2019Quote: ... The viral N-protein was labeled using a primary anti-Tula virus antibody(12) and a secondary anti-rabbit FITC conjugate (Invitrogen, USA). The viral Gc protein was stained using a mouse monoclonal antibody (#H1808-60B ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and PCR amplification with virus candidate specific primers using the Platinum™ SuperFi™ DNA polymerase (Invitrogen™, Catalog number: 12359010). Amplified products were Sanger sequenced using intermediary primers enabling a full-length coverage (all primers are listed in Table S5).
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at approximately 250 × g for 5 min at 4 °C and resuspended in thawed Geltrex™ LDEV ([lactose dehydrogenase elevating virus]-Free Reduced Growth Factor Basement Membrane Matrix; Gibco). Two 15-μl drops of the matrix-cell suspension were pipetted into wells of a Nunc™ 24-well-Nuclon Delta-treated plate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed three times with PBS to remove residual input virus and overlaid with DMEM high glucose media (Life Technologies) with 0.7% low IgG BSA (Sigma) ...
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bioRxiv - Bioengineering 2020Quote: ... vRNA levels were determined by quantitative real-time PCR (qRT-PCR) using Applied BiosystemsTM TaqManTM Fast Virus 1-Step Master Mix (Thermofisher #4444432) and a primer-probe combination recognizing a conserved region of gag (GAG5f ...
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bioRxiv - Plant Biology 2021Quote: ... The identified gene fragments were then PCR-amplified from whitefly cDNA and cloned into a binary tobacco rattle virus VIGS vector pTRV2 (Liu et al., 2002) using the Invitrogen Gateway recombination cloning technology (Invitrogen, USA). As a positive control ...
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bioRxiv - Microbiology 2020Quote: ... apical poles were gently washed with warm PBS and then infected with a 150-μL dilution of HRSV-A (Long) virus in OptiMEM medium (Gibco, ThermoFisher Scientific) at a MOI of 1 ...
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bioRxiv - Microbiology 2020Quote: Zika virus RNA yields were assessed using real-time PCR on a 7500 Fast Real-Time PCR instrument (Thermo Scientific, Poland). ZIKV cDNA was amplified in a reaction mixture containing 1× TaqMan Universal PCR Master Mix (RT-PCR mix ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: RNA extraction and RT-PCR were performed as previously described.45 Total RNA was extracted from HCoV-NL63 virus infected Caco-2 cells at a MOI of 0.05 at 48 hours post infection using TRIzol reagents (Thermo Fisher Scientific). 2.0□μg of total RNA was used to synthesize the first strand cDNA of viral RNA and host RNA using SuperScript III reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: Peripheral blood CD34+CD38- cells mobilized from healthy donors were reprogrammed into iPSCs (MCND-TENS2 line) using the integration-free CytoTune 2.0 Sendai virus reprogramming kit (A16517, Thermo Fisher Scientific) following the method previously reported30,31 ...
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bioRxiv - Neuroscience 2020Quote: Total RNA (~40 μg per reaction) was reverse-transcribed and PCR amplified using Taqman Fast Virus 1-Step Master Mix (Applied Biosystems) and primetime assays for specific genes (IDT) ...
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bioRxiv - Microbiology 2021Quote: ... was derived from Vero cells infected with the virus and cDNA was synthesized using the SuperScript III Reverse Transcriptase (Life Technologies) and oligo dT and six hexamer random primers ...
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bioRxiv - Microbiology 2020Quote: ... viral stocks were prepared after two serial passages of isolated virus in Vero E6 cells in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) supplemented with 5% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... Ebola virus Makona Glycoprotein (EBOV-GP) (KM233102.1) was synthesized and cloned into pcDNA3.1 by GeneArt AG technology (Life Technologies, Regensburg, Germany) or VSV-G following the manufacturer’s instructions of Lipofectamine 3000 (Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The eluted RNA was combined with Applied Biosystems™ TaqMan™ Fast Virus 1-step master mix (ThermoFisher, Waltham, MA, USA). The target regions for the CSG ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of the RNA extraction final elution was reverse-transcribed to cDNA and amplified according to the manufacturer’s protocol using TaqMan™ Fast Virus 1-Step Master Mix (ThermoFisher Scientific). The primer pair was as follows ...
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bioRxiv - Immunology 2020Quote: ... Virus containing supernatants were harvested 2 days later and the produced infectious virus yield was measured using TZM-bl reporter cell line and Gal-Screen kit from Applied Biosystems as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... Induced pluripotent stem cells were derived from human fibroblasts using Sendai virus-based reprogramming kit (CytoTune-iPS Sendai Reprogramming Kit; cat #. A13780-01; Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and number of virus-infected cells (based on dsRNA and nucleocapsid staining) was performed using the CellInsight CX7 HCS platform (Thermo Fisher), as previously described 55,56 ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were co-transfected with the pNL4.3.Luc.R-E-viral vector as well as a plasmid expressing the vesicular stomatitis virus glycoprotein (VSV-G) envelope (pMD2.G) using Lipofectamine 3000 (Life Technologies) according to basic manufacturer’s protocol ...