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Citations for Thermo Fisher :
3851 - 3900 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Total RNA was retrotranscribed with random primer and M-MLV Reverse Transcriptase (Thermo Fisher Scientific, 28025013). qPCR analysis was carried out in a QuantStudio 6 Flex RealTime PCR System (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: Protein extracts from bone were prepared in M-PER buffer (cat#78501, Thermo Fisher Scientific, USA) containing phosphatase and proteinase inhibitors (cat#78442 ...
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bioRxiv - Biochemistry 2022Quote: ... EVD68 2C NΔ39: εmax = 20,860 M-1·cm-1) with NanoDrop One (Thermo Fisher Scientific Inc.). The ionic strength of the dialyzed protein solution was confirmed with a conductivity meter ...
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bioRxiv - Microbiology 2022Quote: ... the cells were lysed in mammalian protein extraction reagent (M-PER; Cat# 78503, Thermo Fisher Scientific) supplemented with Halt protease inhibitor cocktail (Cat# 877886 ...
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bioRxiv - Neuroscience 2022Quote: ... and oncostatin M (OSM) was performed in an ABI7500 Real-Time PCR System (Thermo Fisher Scientific) in combination with TaqMan® chemistry ...
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bioRxiv - Plant Biology 2022Quote: ... for 30 min at 37°C and transcribed using the M-MLV-Reverse Transcriptase (ThermoFisher Scientific) with random hexamer primers according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (pH = 8.5) (ThermoFisher Scientific, Waltham, MA), 4-benzoylbenzyl-trimethylammonium chloride (PLPP ...
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bioRxiv - Biochemistry 2024Quote: ... The filtrate was diluted two times the volume with buffer UA (8 M urea [Acros Organics, CAS No ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were alkalised with 1 M TEAB prior to labelling with TMTpro 18-plex (ThermoFisher) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 500 ng of total RNA was reverse transcribed using the M-MLV RT kit (28025013; Invitrogen). TaqMan Fast Advanced Master Mix (4444557 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Extracted ion chromatograms of the [M+H]+ forms were integrated using Tracefinder software (Thermo Fisher Scientific). For the reported intensity levels of the different amino acids ...
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bioRxiv - Genomics 2024Quote: ... DNA fragments were then denatured and bound to magnetic streptavidin M-280 beads (Invitrogen cat. 11205D) for 30 minutes at room temperature with nutation ...
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HOIL-1L deficiency induces cell cycle alteration which causes immaturity of myocyte and fibrogenesisbioRxiv - Cell Biology 2023Quote: Myotubes at day 5 of differentiation were lysed in M-PER buffer (Thermo Scientific, Cat.# 78501) containing 1× protease inhibitor and then incubated on ice ...
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bioRxiv - Neuroscience 2023Quote: ... 0.25 M sucrose) on ice and incubated with 1 mg/ml of fluorescein-dextran (Invitrogen, D1823) for 10 minutes at room temperature ...
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bioRxiv - Genetics 2023Quote: ... 694.0285 and 694.3614 m/z) were summed for quantitative analysis in the Quan Browser program (ThermoFisher). The plasma samples from mothers with heterozygous fetuses were analysed on an M-Class LC system (Waters) ...
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bioRxiv - Molecular Biology 2023Quote: Cells were rinsed with ice-cold PBS and lysed in M-PER buffer (Thermo Fisher Scientific) containing Halt protease and phosphatase inhibitor cocktail (1X ...
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bioRxiv - Neuroscience 2023Quote: ... the cells were lysed for 5 min with M-PER mammalian protein extraction reagent (Thermo Scientific) including Halt protease inhibitor (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... and the remaining samples were incubated with Dynabeads M-280 streptavidin magnetic beads (Invitrogen, cat # 11206D) for 2 h at 4°C on an end-over-end rotator ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 7.4 0.15 M NaCl 0.1% Tween 20) with 1:100 protease inhibitor (78442, Thermo Scientific). The lysates were denatured by boiling ...
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bioRxiv - Cell Biology 2023Quote: ... Ltd.) and 1/100 (v/v) of 1 M HEPES buffer (15630–106, Thermo Fisher Scientific) was added to the collected cells ...
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bioRxiv - Cell Biology 2023Quote: Whole cell lysates were prepared in M-PER buffer (1x Mammalian Protein Extraction Reagent (Thermo Scientific), 0.1% Halt Protease & Phosphatase inhibitor (Thermo Scientific ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the complementary cDNA was synthesized with an M-MLV Reverse transcriptase (Thermo Fisher Scientific, Waltham, MA). The synthesized cDNA was used as a template for qRT-PCR reactions ...
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bioRxiv - Cell Biology 2023Quote: ... A 10X biotinylation buffer was prepared by diluting 1 M Tris-HCl pH 7.5 (ThermoFisher 15567027) to 100 mM and 0.5 M EDTA pH 8 (ThermoFisher AM9261 ...
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bioRxiv - Developmental Biology 2023Quote: ... directly into a PCR plate containing 15 µL 1 M ammonium bicarbonate (Acros Organics, Geel, Belgium) solution by centrifugation (4 min ...
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bioRxiv - Bioengineering 2023Quote: ... 1 μL M-MLV Reverse Transcriptase (RT) (200 U/μL, Invitrogen, 28025-013, Breda, the Netherlands) and supplemented with RNAse-free ultra-pure water (ddH2O) ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated with DAPI (1 μ /ml in 0.1 M phosphate buffer, Invitrogen #D1306, USA) for 15 min dark ...
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bioRxiv - Genetics 2023Quote: ... blood was transferred to cryovials containing 0.5 M Perchloric acid acs reagent 70% (Thermo Fisher Scientific) and stored at -80°C until further processing ...
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bioRxiv - Immunology 2022Quote: ... with program RNA_02 in M tubes (#130-096-335) in 1 ml of Trizol (Invitrogen, #15596018). The homogenates were centrifuged at 2000 g for 1 minutes at 4°C and the supernatant was collected ...
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bioRxiv - Bioengineering 2023Quote: ... ACLY and/or ACSS2-KO cell lines were lysed in M-PER buffer (Thermo Scientific, 78501) with 1x protease inhibitor (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed in TNT buffer comprised of dH2O containing 0.15 M NaCl (Fisher Scientific), 0.1 M Tris HCl (pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: HEK-293Ts transfected with the indicated plasmids were lysed in M-PER lysis buffer (Thermo Scientific) supplemented with EDTA-free protease inhibitor (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Whole cell lysates were prepared in M-PER buffer (1x Mammalian Protein Extraction Reagent (Thermo Scientific), 0.1% Halt Protease & Phosphatase inhibitor (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... and cDNA was synthesized from 1 μg of total RNA using the M-MLV (Invitrogen; 28025013). SYBR green master mix (Roche ...
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bioRxiv - Physiology 2023Quote: ... The slides were then incubated in acidified water made of 0.05 M hydrochloric acid (Fisher Scientific) for 90 seconds ...
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bioRxiv - Physiology 2023Quote: ... Isolated RNA was reverse-transcribed into cDNA using M-MLV Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA) as described previously (19) ...
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bioRxiv - Cancer Biology 2023Quote: ... MOC1 and MOC2 cell lines were grown in DMEM supplemented with 10% M-FBS (Gibco, Mexico). All cell lines were profiled using short tandem repeats (STR ...
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bioRxiv - Molecular Biology 2023Quote: ... Whole cell lysates were prepared in M-PER buffer (1x Mammalian Protein Extraction Reagent (Thermo Scientific), 0.1% Halt Protease & Phosphatase inhibitor (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... N2A or NIH3T3 cells were plated 2000 cells/m and cultured in DMEM high glucose (GIBCO) containing 10% fetal calf serum ...
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bioRxiv - Immunology 2023Quote: ... 200ng of RNA was used to synthesize cDNA with the M-MLV Reverse Transcriptase kit (Invitrogen). Il23a transcript expression was quantified using Il23a (FAM labelled ...
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bioRxiv - Genomics 2024Quote: ... Cells were grown in culture in M-199 (ThermoFisher Scientific, Waltham, MA, MT-10-060-CV) supplemented with 1.2% sodium pyruvate (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... the glycopeptides were eluted twice using 200 µL of 0.8 M galactose (Thermo Fisher Scientific, A12813.18) prepared in 20 mM Tris by rotation at 4 ºC for 30 minutes each time ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant proteins were purified under denaturing conditions (8 M urea) using Ni-NTA resin (Thermo Scientific). The purity of the recombinant proteins was assessed via SDS-PAGE ...
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bioRxiv - Microbiology 2024Quote: ... This solution was mixed with 0.4 mL of Dynabeads M-280 Sheep Anti-Mouse IgG (Invitrogen) previously conjugated with 25 ug of S9.6 antibody (Millipore ...
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bioRxiv - Cancer Biology 2021Quote: ... The following secondary antibodies were used: goat α-rat Alexa Fluor 647 (1:1000; A21247; Invitrogen), goat α-rabbit Alexa Fluor 568 (1:1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies goat anti-rat Alexa Fluor 488 and goat anti rabbit Alexa Fluor 647 (Invitrogen). Sections were counterstained with SYTOX-Green at 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... CD11b+ microglia and macrophages were identified using rat anti-CD11b antibodies (ThermoFisher, cat#14-0112-82). GFAP was stained using rabbit anti-GFAP antibodies (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... Slides were then incubated with Alexa Fluor 594 goat anti-rat antibody (Thermo Fisher; 1:100) for 1 h ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit and rat and Alexa Fluor 532 conjugated to antibodies raised against mouse and rabbit (Invitrogen) were used at 1/200 dilution in the saturation media ...
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bioRxiv - Evolutionary Biology 2021Quote: ... followed by a secondary anti-rat antibody coupled to AlexaFluor 594 fluorophores (dilution 1:200, Invitrogen). For co-localization experiments with ER or mitosomal markers ...
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bioRxiv - Microbiology 2021Quote: ... They were then incubated with the secondary antibodies AlexaFluor 488 conjugated donkey α-rat (Life Technologies) and AlexaFluor 488 conjugated goat α-mouse (Invitrogen/Thermo Fisher ...