Labshake search
Citations for Thermo Fisher :
3751 - 3800 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... Secondary antibodies that were used were Alexa488 (goat anti-rat, Invitrogen Cat No: A-11006), Alexa568 (goat anti-mouse ...
-
bioRxiv - Developmental Biology 2024Quote: ... cells were stained with rat anti-Sox2 (1:1000, Invitrogen, Cat. No. 14-9811-80), goat anti-Olig2 (1:1000 ...
-
bioRxiv - Synthetic Biology 2024Quote: ... and coated with 500 µL rat tail collagen I (Thermo Fisher Scientific, catalog no. A1048301) at a concentration of 50 µg/mL diluted in 25 mM acetic acid ...
-
bioRxiv - Developmental Biology 2024Quote: ... and incubated in anti-rat secondary antibody [1:400 (Thermo Fisher Scientific, Alexa Fluor 594)] diluted in WB2 for 36 h ...
-
bioRxiv - Cell Biology 2024Quote: ... [anti-rat 488 secondary antibody (H+L) 1:400 (A21208 Thermo Scientific, Alexa Fluor 488); anti-rabbit 568 secondary antibody (H+L ...
-
bioRxiv - Cell Biology 2024Quote: ... and a 1:5000 dilution of donkey anti-rat DyLight800 (Invitrogen Life Technologies SA5-10032). Membranes containing GFP-tagged proteins were probed with a 1:1000 of goat anti-GFP polyclonal antibody (Abcam ab5450 ...
-
bioRxiv - Neuroscience 2024Quote: ... Slices were then stained with 300 µL of anti-rat-Alexa568 secondary antibody (A78946, Invitrogen) at 1:500 dilution in 1% BSA/PBST for 2 hours on a shaker at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... for 10 min at 37°C/5% CO2 before rat primary cortical astrocytes (Gibco, #N7745100) were plated at a density of 100 cells/mm2 48 hours prior to plating of neurons ...
-
bioRxiv - Neuroscience 2024Quote: ... with the exception of a rat monoclonal antibody specific for mCherry (1:25, M11217, Invitrogen). Following overnight blocking at 4°C with 0.2% normal goat serum in detergent-free phosphate buffer ...
-
bioRxiv - Cell Biology 2024Quote: ... and a 1:5000 dilution of donkey anti-rat DyLight800 (Invitrogen Life Technologies SA5-10032). Membranes containing GFP-tagged proteins were probed with a 1:1000 of goat anti-GFP polyclonal antibody (Abcam ab5450 ...
-
bioRxiv - Neuroscience 2024Quote: ... The following secondary antibodies were used: Goat α-rat Alexa 647 (Molecular probes A-21247) and Goat α-Mouse Alexa 488 (Molecular probes A11029 ...
-
bioRxiv - Cell Biology 2024Quote: ... The primary antibodies were used as follows: rat anti-tubulin (1:500, MA1-80017, Invitrogen) and human anti-centromere protein (1:300 ...
-
bioRxiv - Immunology 2024Quote: ... rat or rabbit IgG (Invitrogen Clone A10043 Lot 753765; Invitrogen Clone A11055 Lot 2211210; Invitrogen A21447 Lot 872645 ...
-
bioRxiv - Molecular Biology 2024Quote: ... sections were incubated with goat anti-rat biotin (1:500) (catalog #: 31830; Thermo Fisher Scientific) in 2.5% BSA for 90 minutes ...
-
bioRxiv - Neuroscience 2024Quote: ... Primary astrocytes obtained from E18 rat cortices via 15 min trypsinisation (10 mg/ml; Gibco), were plated 1 week prior and treated with culture ONE supplement (Gibco ...
-
bioRxiv - Physiology 2024Quote: ... donkey anti-rat Alexa Fluor 647 (6.67 µg/mL, Cat. # A-78947; Invitrogen, Darmstadt, Germany) or Alexa Flour 647-conjugated streptavidin (6.67 µg/mL ...
-
bioRxiv - Physiology 2024Quote: ... and donkey anti-rat DyLight 550 (5 µg/mL, Cat. # SA5-10027; Invitrogen, Darmstadt, Germany) for 1 hour followed by incubation with Hoechst (2 µmol/L ...
-
bioRxiv - Immunology 2024Quote: ... microglia (Iba1) followed by anti-rat CF-647- (Biotrium) and anti-rabbit AlexaFluor 568- (Invitrogen) labelled secondary antibodies ...
-
bioRxiv - Neuroscience 2024Quote: ... then incubated in secondary antibodies [Goat anti-Rat Alexa Fluor 647 (1:1,000; Invitrogen; A21247),Goat anti-Mouse Alexa Fluor 488 (1:1,000 ...
-
bioRxiv - Physiology 2024Quote: ... BrdU-positive cells were visualized using Alexa Fluor 594 anti-rat secondary antibody (Life Technologies) and DAPI (Life Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... and goat anti-rat-Alexa Fluor 488 (1:800, Thermo Fisher Scientific, A-11006, RRID:AB_2534074).
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were subsequently incubated for 2 hours at 4°C with Streptavidin Dynabeads (Invitrogen M-280), and beads were successively washed with RIPA (2 times) ...
-
bioRxiv - Cell Biology 2020Quote: ... The lysate was then incubated with epoxy-activated M-270 Dynabeads (Thermo Fisher Scientific, Cat#14305D), which were cross-linked with rabbit IgG (Sigma-Aldrich ...
-
bioRxiv - Immunology 2021Quote: ... Total RNA (200-400 ng) was reverse transcribed using the M-MLV Reverse Transcriptase kit (ThermoFisher). Real-time qPCR runs were performed on a CFX96 Real-time C1000 thermal cycler (Biorad ...
-
bioRxiv - Genetics 2021Quote: RNA was reverse transcribed into cDNA using the Moloney Murine Leukemia Virus (M-MLV; Thermo Fisher) according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... including the DNase treatment and were precipitated by adding 1:1 LiCl (7.5 M) (Invitrogen AM9480) and incubating at −20°C for 30 minutes followed by centrifugation at full speed at 4°C for 15 minutes and extensive EtOH washes ...
-
bioRxiv - Microbiology 2022Quote: ... or by matching accurate mass and MS2 fragments to m/z cloud database (Thermo Fisher Scientific). Data were exported to Excel for grouping ...
-
bioRxiv - Molecular Biology 2021Quote: ... Biotinylated RNA was precipitated with chloroform/isopropanol and incubated with Dynabeads M-270 Streptavidin (Thermo Fisher) for 1 hour at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: Reverse transcription was performed according to manufacturer’s instructions using M-MLV reverse transcriptase (Invitrogen, Ref. 28025013) and qPCR was performed using Applied Biosystems Power up SYBR Green PCR Master mix following the manufacturer’s instructions and using an Applied Biosystems QuantStudio 3 Real-Time PCR System ...
-
bioRxiv - Molecular Biology 2021Quote: ... resuspended in manufacturer-recommended solvents were coupled to 10 µL Dynabeads M-280 streptavidin beads (Invitrogen) in 250 µl peptide pulldown buffer 1 (PPB1 ...
-
bioRxiv - Molecular Biology 2019Quote: ... 20 μl of 5 M NaCl and 10 μl of 10 mg/ml proteinase K (Invitrogen) was then added to the input ...
-
bioRxiv - Immunology 2021Quote: ... Phage display was performed on biotinylated RBD immobilized on streptavidin magnetic beads (Dynabeads M-280, Invitrogen) or strep-tagged spike immobilized on Strep-Tactin XT magnetic beads (IBA Lifesciences) ...
-
bioRxiv - Cell Biology 2020Quote: ... SKOV3-Red and SKOV3-M were resuspended in Hanks’ Balanced Salt Solution (Life Technologies, Carlsbad, USA) and incubated with 1 μM CellTrace Far Red (Life Technologies ...
-
bioRxiv - Cancer Biology 2019Quote: ... cDNA was synthesized using an M-MLV Reverse Transcriptase Enzyme System (Life Technologies, Thermo Fisher Scientific) and OligodT primers ...
-
bioRxiv - Cancer Biology 2019Quote: ... cDNA was synthesized using an M-MLV Reverse Transcriptase Enzyme System (Life Technologies, Thermo Fisher Scientific) and OligodT primers ...
-
bioRxiv - Genetics 2020Quote: ... Pulldowns were performed with 1.2 mg of nuclear extracts using M-280 paramagnetic streptavidin beads (Invitrogen) as previously described (Chatzinikolaou et al. ...
-
bioRxiv - Genetics 2019Quote: ... 500μM of dNTP mix and 100U of M-MuLV reverse transcriptase (SuperScript III Reverse Transcriptase, Invitrogen) were added to a final reaction volume of 20μl followed by incubation for 1h at 37°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... The purified pellet was dissolved in 0.1 M TEAA (triethylammonium acetate) (Thermo Fisher Scientific cat# 400613).
-
bioRxiv - Bioengineering 2019Quote: ... 1 μL M-MLV Reverse Transcriptase (200 U μL−1, Invitrogen, 28025-013, Breda, the Netherlands), and RNAse-free ultra-pure water (ddH2O ...
-
bioRxiv - Plant Biology 2019Quote: ... the targets were captured by the Dynabeads M-270 Streptavidin beads (65-305, Thermo Fisher Scientific) that recognize the dualbiotinylated probe ...
-
bioRxiv - Cancer Biology 2020Quote: ... supplemented with 10% FBS (0.01 M HEPES, 1x sodium pyruvate, 0.05 mM 2-beta-mercaptoethanol (Gibco) and 0.1 mg/ml Penicillin-streptomycin (Sigma ...
-
bioRxiv - Animal Behavior and Cognition 2019Quote: ... DEPC treated water and Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) kit (Applied Biosystems, 850 Lincoln Centre Drive ...
-
bioRxiv - Developmental Biology 2019Quote: ... a cDNA library was prepared using M-MLV Reverse Transcriptase with oligo(dT) primer (ThermoFisher Scientific). Intron-spanning amplicons from pigeon PITX1 ...
-
bioRxiv - Immunology 2021Quote: cDNA synthesis was performed using the First-strand cDNA Synthesis M-MLV Reverse Transcriptase Kit (Invitrogen) protocol ...
-
bioRxiv - Systems Biology 2021Quote: ... cells were lysed using M-PER or Mem-PER mammalian protein extraction reagents (Thermo Fisher Scientific) and blotted onto PVDF membranes as described above ...
-
bioRxiv - Bioengineering 2021Quote: ... Human GAA coding sequence variant GILTco2-m was designed using GeneArt codon optimization algorithm (Thermofisher Scientific). The human GAA coding sequence variant GILTco3-m was designed using a consensus sequence (CCDS32760.1) ...
-
bioRxiv - Cell Biology 2021Quote: ... 100pmol biotinylated peptides (Peptide 2.0) were bound to 8μl streptavidin M-280 Dynabeads (Thermo Fisher Scientific) for 30mins in PBS at room temperature with agitation ...
-
bioRxiv - Neuroscience 2020Quote: ... Mφ were exposed to 100 nM of MitoTracker Green FM (M-7514, ThermoFisher scientific, Massachusetts, USA) for 10 min and then washed with media ...
-
bioRxiv - Pathology 2021Quote: ... previously coupled to Dynabeads® M-270 Epoxy using Dynabeads® Co-Immunoprecipitation Kit (Life Technologies) as described by the manufacturer ...
-
bioRxiv - Microbiology 2021Quote: ... pneumococcal-specific Abs were detected using horseradish peroxidase–conjugated goat anti-mouse immunoglobulin M (IgM; Invitrogen), IgG (Millipore Sigma) ...