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Citations for Thermo Fisher :
3801 - 3850 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... or ParB-EDTA buffer (by replacing the 4 mM MgCl2 with 1 mM EDTA) and then incubated with 1 μm diameter magnetic beads (Dynabeads, Myone streptavidin, Invitrogen) for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... A second round of reverse transcription was initiated with the addition of 4 µL of reverse transcription mix (4:2:1:1 of First Strand Buffer, 0.1 M DTT, RNaseOUT, SuperScript II; ThermoFisher Scientific) to each sample then incubated at 25□ for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26; 1:250, Invitrogen; Rabbit anti-Cx30 ...
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bioRxiv - Pathology 2020Quote: ... The aqueous phase was separated by centrifugation (21,000 g for 15 min at 4 °C) and incubated with isopropanol (1:1 ratio) and 25 μg of Glycoblue (ThermoFisher) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... sections were blocked in 5% normal goat serum + 2% BSA and 2% Triton-X for 1 h and incubated with the following primary antibodies overnight at 4°C: rabbit anti-GFP (1:1000, Invitrogen) and chicken anti-TH (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and then stained with HERV-K envelope primary antibodies (1:1000) (Ango) overnight at 4°C and Alexa Fluor 488 secondary antibodies (1:500) (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... was performed at 30V for 1 hour at 4°C in 1X NuPAGE™ buffer (REF NP0006-1, Life Technologies). After transfer ...
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bioRxiv - Microbiology 2022Quote: Loss of function of NEU1-4 was conducted by transfecting cells with pooled siRNAs (Supplemental Table 1) (Dharmacon) or single siRNAs (Supplemental Table 1) (ThermoFisher) using RNAiMAX (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were first incubated with rat anti-HA (1:100) antibody overnight at 4°C followed by donkey anti-rat Alexa Fluor (AF) 488 (1:500; Invitrogen) for 1.5 h at 20°C before staining for other markers ...
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bioRxiv - Physiology 2022Quote: ... Cell surface staining was done in live cells blocked with diluted superblock (1:4 dilution in PBS with 1 mM MgCl2 and 0.1 mM CaCl2, ThermoFisher Scientific) at 4°C for 0.5 h ...
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bioRxiv - Molecular Biology 2024Quote: ... mono-Methylated K35 overnight (1:100) at 4°C followed by 1:100 Alexa-Fluor 488 and Alexa-Fluor 555 (both Invitrogen) for 2 h at RT.
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bioRxiv - Cancer Biology 2024Quote: ... the Live/Dead assay was conducted using 1 μM calcein AM and 4 μM ethidium homodimer-1 (Life Technologies kit) for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 45 minutes with 1:500 AlexaFluor 488-conjugated anti-mouse (Invitrogen, 1:500 in 4% BSA PBS) plus DAPI (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... RPMI 1640 containing 2 μM calcein AM and 4 μM EthD-1 along with 1 mg/mL DAPI (ThermoFisher, 62248) was added to the 384-deep well plate (80 μL/well) ...
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bioRxiv - Bioengineering 2023Quote: ... 105 rested NY-ESO-1 TCR+ T cells (4:1 CD8+:CD4+) were labeled with 0.5uM CSFE (Thermo Fisher Scientific) and co-cultured with 105 NucLight red+ tumor cells in complete medium ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated for 2 hours at room temperature with secondary antibodies in PBS (Donkey anti-Mouse 647 nm 1:1000, Abcam ab15107, lot GR3234832-4; Donkey anti-Rabbit 594nm 1:1000, Invitrogen A21207 ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated overnight at 4 °C with the following antibody: ERα (Dilution 1:1 µg/mL; Invitrogen, CAT: MA5-13191). IRDye secondary antibody (Dilution 1:20,000 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with primary antibodies overnight at 4°C in a shaker (Cofilin Total 1:500 Invitrogen #MA517275; Phosphorylated-cofilin 1:500 Invitrogen #44-1072G and β-actin 1:3000 ...
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bioRxiv - Immunology 2023Quote: ... The upper aqueous layer was removed to a new tube and mixed 1:1 with Isopropanol (Fisher Scientific, BP2618-4) and incubated for 1 min with glycoblue co-precipitant (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Two skin swab samples were taken in a 5 mL centrifuge tube and mixed with 1 mL of pre-cooled methanol-water (V:V=4:1, Thermo Fisher), after brief sonication in ice water for 20 min ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were harvested by centrifugation at 4°C and resuspended in 40 mL 1:1 PBS:Bacterial Protein Extraction Reagent (BPER, ThermoFisher Scientific). Cell pellets were lysed by incubating in 60°C water bath for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... One-step RT-PCR using a SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase (Thermo Fisher Scientific, Waltham, MA, USA) was performed using a combination of primers [Mar-CPL-F and MarCP2R (876 bp)] designed specifically for minor CP amplification.
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bioRxiv - Genomics 2024Quote: ... The quality of the extracted RNA was validated with the NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermo Scientific™ Cat. No. ND-ONE-W) and 1% agarose gel electrophoresis ...
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bioRxiv - Neuroscience 2019Quote: ... blocked for 30 min with 1% BSA in PBS-Tween (0.1%) and then incubated for 1 h with the primary antibodies (the lysosomal marker LAMP-1 (Invitrogen, 4-1071-82, 1:500) or the microglia marker IBA1 (Wako ...
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bioRxiv - Neuroscience 2023Quote: ... 1:200) at 4 °C 48 hours and in secondary antibodies simultaneously (Goat Anti-mouse 1:200, Invitrogen, Goat Anti-Rabbit 1:200, Jackson) at RT for 1 hour ...
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bioRxiv - Bioengineering 2022Quote: ... coliΔXbaI cell cultures are both induced with 0.5 mM (final concentration) of Isopropyl β-D-1-thiogalactopyranoside (IPTG; Invitrogen), and incubated at 37 °C in the dark for a given period of time before being disrupted ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in fresh DMEM-10 (Gibco DMEM #11995-065 with 4.5 g/L D-Glucose and 110 mg/L Sodium Pyruvate, 10% Fetal Bovine Serum [HyClone], 1% HEPES [Gibco 1M 15630-080] ...
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bioRxiv - Cell Biology 2020Quote: ... referred to herein as INS-1 832/13) were cultured in RPMI 1640 medium with 11.1 mM D-glucose (Invitrogen) supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured adherently in DMEM (4.5 g/L D-Glucose) supplemented with 1× MEM NEAA (catalog no. 11140-035, Gibco), 1× GlutaMAX (catalog no ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then induced by the addition of 0.5 mM Isopropyl B-D-1-thiogalactopyranoside (IPTG, INVITROGEN Life Technologies) and further grown at 21°C for 16-18 h ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then induced by the addition of 0.5 mM Isopropyl B-D-1-thiogalactopyranoside (IPTG, INVITROGEN Life Technologies) and further grown at 21°C for 16-18 h ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmids pCAG-dsRed and Pak3K297R transfection was performed at 7 d in vitro (DIV) with 1 µg of DNA and 1.5 µl of Lipofectamine (Invitrogen), as described in manufacturer’s manual (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: Prion-infected brain homogenate was prepared by homogenizing 200 brains from CD-1 mice terminally-infected with the RML prion strain in Dulbecco’s phosphate buffered saline (D-PBS; Gibco) to produce a pool of ~1 litre 10 % (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... HEK 293T and Flp-In HEK293 T-REx were maintained in DMEM-GlutaMAX at 4.5 g*ml-1 D-glucose (Gibco) supplied with sodium-pyruvate ...
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bioRxiv - Cancer Biology 2020Quote: ... U2OS and HEK-239T cells were grown in DMEM containing 2.5 mM L-glutamine and 4.5 g l-1 D-glucose (Life Technologies). H1299 cells were grown in RPMI 1640 medium containing 2.5 mM L-glutamine.
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bioRxiv - Immunology 2022Quote: ... BCRJ 0234) cell lines were maintained at 37ºC and 5% CO2 in DMEM (1 g/L D-glucose, GIBCO) supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were cultured in Dulbecco’s Modified Eagle Medium with 1 g/L D-glucose (low-glucose DMEM; Invitrogen, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Bioengineering 2024Quote: A total of 5 × 103 Hek293T and HeLa cells were put into individual wells containing Dulbecco’s Modified Eagle Medium (D-MEM) [containing 10% Fetal Bovine Serum (FBS) and 1% Antibiotic-Antimycotic solution (Gibco)] ...
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bioRxiv - Cancer Biology 2023Quote: ... Anti-NKp46 and NKp30 agonist antibodies (R&D) were coated (1 µg/mL) in NUNC 96-well plates (Thermofisher) overnight at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... We used carboxy-terminated microspheres that exclusively were found to enter inside the condensates (d = 1 µm, Thermofisher, F8816).
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bioRxiv - Neuroscience 2023Quote: ... their columns were excised in a dissection medium constituted of a solution of D-glucose 6.5 mg ml-1 in DPBS (Gibco; Thermo Fisher Scientific ...
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bioRxiv - Genetics 2023Quote: ... were grown on 10-mm coverslips previously coated with poly-D-lysine (0.1 mg/mL, 1 h, 37 °C, Gibco) placed in a well on a 24-well plate ...
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bioRxiv - Microbiology 2024Quote: ... coli was induced by the addition of 1mM of Isopropyl ß-D-1-thiogalactopyranoside (IPTG) (Invitrogen™, MA, USA). The protein extraction and purification were performed by B-PER™ with Enzymes ...
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bioRxiv - Microbiology 2020Quote: ... The wells were washed 3 times with 1 mL PBS 1X and covered with a mixture containing secondary goat anti-rabbit-Alexa488 antibodies (Invitrogen Molecular Probe 2mg.mL-1, 1/300e) and DAPI (1/100e ...
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bioRxiv - Plant Biology 2020Quote: ... One ml of HisPur™ Ni-NTA Superflow Agarose (ThermoFisher Scientific) slurry was added to the extract and incubated for 15 min at 4°C with shaking ...
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bioRxiv - Plant Biology 2020Quote: ... one tablet/50 mL EDTA free Pierce protease inhibitor (Thermo Fisher)) at 2:1 ratio (2 mL buffer ...
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bioRxiv - Microbiology 2019Quote: ... one or two million cells suspended in CO2-independent medium (Gibco) supplemented with 10% FBS were mixed with VSV-G-pseudotyped virus stocks and shaken at 220 rpm for 2 h at 37 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... and quality was assessed with NanoDrop™ One (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... One hour prior to fixation 10 µM ethylene-deoxyuridine (EdU, Thermofisher) was added to the media ...
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bioRxiv - Molecular Biology 2021Quote: ... the concentration determined with a NanoDrop™ One spectrophotometer (ThermoFisher Scientific), and stored at −80°C until further use.