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Citations for Thermo Fisher :
3601 - 3650 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Ethanol was aspirated and cells were incubated with 1 M Tris pH 8.0 (Thermo Scientific) for 5 min ...
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bioRxiv - Bioengineering 2022Quote: ... HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) 1 M was purchased from Gibco (Waltham, MA), sodium chloride 5 M was purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... and/or nesprin-1 siRNA (M-014039-02-0005, Dharmacon) using Lipofectamine 2000 (11668027, Invitrogen) or Lipofectamine 3000 (L3000015 ...
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bioRxiv - Bioengineering 2022Quote: ... silk was dissolved in 9 M LiBr (199870025, Acros, Thermo Fisher Scientific, Breda, The Netherlands), filtered ...
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bioRxiv - Physiology 2023Quote: ... The subsequent blood samples were immediately mixed with 57 µl of 0.1 M PBS (Invitrogen) containing 0.05% Tween 20 and 0.2% BSA (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2024Quote: ... 50 µL of Dynabeads™ M-280 Sheep Anti-Rabbit IgG (Thermo Fisher Scientific, #11203D) was added to each tube followed by incubation at 4°C for 2 hours ...
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bioRxiv - Systems Biology 2024Quote: ... Abs were first buffer exchanged into 0.2 M NaHCO3 (pH 8.3) (Thermo Scientific Acros 217125000) by using Zeba™ Spin Desalting Columns ...
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bioRxiv - Physiology 2024Quote: ... which was reverse transcribed to cDNA with the M-MLV Reverse Transcriptase kit (Life Technologies). We performed RT-qPCR in a 7500 Fast Real Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... The following day tissue was embedded in Shandon M-1 Embedding Matrix (Thermo Fisher Scientific) and snap frozen in liquid nitrogen ...
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bioRxiv - Cancer Biology 2024Quote: Cells were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Scientific™, Cat# 78501) supplemented with Protease Inhibitor Cocktail and Halt Phosphatase Cocktail (Thermo Scientific™ ...
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bioRxiv - Genomics 2024Quote: 0.5 M EDTA pH 8.0 (Invitrogen™/Thermo Fisher, Carlsbad, CA, USA; cat. no. AM9261)
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bioRxiv - Immunology 2024Quote: ... 10% heat inactivated fetal bovine serum (HIFBS) 2 × 10-3 m L-glutamine (Life Technologies), antibiotic antimycotic (1× ...
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bioRxiv - Immunology 2024Quote: Human IgG (hIgG) was coupled to M-450 Epoxy Dynabeads (cat# 14011, Thermo Fisher Scientific) according to the product manual ...
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bioRxiv - Immunology 2024Quote: ... according to manufacturer instructions and labeled with 5 M carboxyfluorescein succinimidyl ester (CFSE, Thermofisher Scientific) for 10 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... 11 µl per sample of Dynabeads® M-280 sheep anti-mouse IgG (Thermo Fisher) were collected with a magnetic rack and pre-washed with 500 µl PBS-BSA (1 mg/ml BSA in 0.1 M PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Whole-cell protein lysates were prepared in M-PER buffer (Thermo Scientific, Rockford, IL, USA) with 1x Protease & Phosphatase Inhibitor Cocktail (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... coupled to 200 µL magnetic beads (Dynabeads M-280 Sheep Anti-mouse IgG, Invitrogen, 11201D) and incubated at 4°C overnight ...
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bioRxiv - Bioengineering 2024Quote: ... were prepared by washing 70 uL of Tosyl-activated M-280 magnetic dynabeads (ThermoFisher #14203), 3 x with 1 mL of PBS for 5 min ...
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bioRxiv - Immunology 2023Quote: ... Co-immunoprecipitation experiments were then carried out using DynabeadsTM M-280 streptavidin (Thermo Fisher Scientific). To allow time for interaction ...
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bioRxiv - Genetics 2023Quote: Protein lysates were obtained by homogenizing tissues in M-PER lysis buffer (ThermoFisher; Cat# 78501) with 1x Complete Mini EDTA-free protease inhibitor cocktail tablets (Roche Applied Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... 80,000 M-1·cm-1) against the protein concentration determined using Bradford protein assay (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: ... and reverse transcribed into cDNA using oligo dT primers and M-MLV Reverse Transcriptase (Invitrogen). Real-time PCR was performed on Quant Studio 6 Flex Real-time PCR system (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation with magnetic streptavidin beads (Dynabeads™ M-280 Streptavidin, Thermo Fisher Scientific) for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... or 40 µl DiD solution (0.5 m/ml in 0.9% saline, D7757, Thermo Fisher Scientific) via PITI on two consecutive days ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting extract was added to magnetic beads (Life Technologies, Dynabeads M-270 Epoxy, 14302D) coupled to rabbit IgG (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... Dried purified silk fibroin was dissolved in 9 M lithium bromide (LiBr, Acros organics, 199870025) solution in UPW at 55°C for 1 hour and dialyzed against UPW for 36 hours using SnakeSkin Dialysis tubing (molecular weight cut-off (MWCO) ...
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bioRxiv - Biophysics 2023Quote: ... 1 M NaCl was incubated with 100 μg succinimidyl ester of AlexaFluor-647 (ThermoFisher, A20006) in the dark for 1 h at 25°C with gentle shaking ...
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bioRxiv - Molecular Biology 2022Quote: ... for COMP-Ang1 IP were covalently coupled to M-270 Epoxy Dynabeads™ (Thermo Scientific), cat ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was generated from 1 μg of total RNA using M-MLV reverse-transcriptase (Invitrogen) and random hexamers (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... scaled up accordingly) M-270 streptavidin-coated Dynabeads (10 µL containing 100 µg, Thermo Fisher) were equilibrated and washed with 1xBW buffer (5 mM Tris-HCl pH 7.5 ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Fragments containing the biotinylated adapter were purified with Dynabeads™ M-280 streptavidin (Thermo Fisher). The end-repair reaction was done using the End-it DNA end-repair kit (Lucigen) ...
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bioRxiv - Bioengineering 2023Quote: ... we added 200 μL of M-PER lysis buffer (Thermo Fisher Scientific Inc., catalog #78503) on top of cells and incubated for 5 minutes at 180 rotations per minute (RPM ...
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bioRxiv - Microbiology 2023Quote: ... 1 M lithium acetate and 10 mg/mL denatured herring sperm DNA (Thermo Fisher Scientific) was added and incubated overnight shaking at 30 °C ...
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bioRxiv - Cell Biology 2023Quote: Mouse cDNA was synthesized by reverse transcription from kidney RNA by M-MLV (ThermoFisher 28025013) with random primers (Invitrogen 48190011) ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was produced from 5 µl of each sample using M-MLV reverse transcriptase (Invitrogen) and random hexamers by the following program ...
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bioRxiv - Molecular Biology 2024Quote: ... protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific Inc, 78501), and Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific Inc ...
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bioRxiv - Immunology 2024Quote: ... 10% heat inactivated fetal bovine serum (HIFBS) 2 × 10−3 m L-glutamine (Life Technologies), antibiotic antimycotic (1× ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 1% 0.5 M EDTA solution and 1% protease and phosphatase inhibitor (Thermo Scientific). The lysate was centrifuged at 14,000 rpm for 15 minutes ...
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bioRxiv - Biophysics 2020Quote: ... and incubated 45 min with secondary goat-anti-rat-Alexa647 antibody (ThermoFisher A21247, 1/800) and kept in PBS before dSTORM imaging.
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bioRxiv - Cancer Biology 2021Quote: ... or a goat-anti-rat Alexa Fluor 488-labeled secondary antibody (1:100; Life Technologies). Cell nuclei were stained with Hoechst 33342 (Sigma-Aldrich) ...
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bioRxiv - Developmental Biology 2021Quote: ... 1:1000 secondary antibody (Goat anti-Rat 488 [Invitrogen, A11006], Goat anti-Rabbit 594 [Invitrogen, A11037] ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration from rat plasma was quantified using bicinchoninic acid protein assay (ThermoFisher Scientific, USA). Samples were diluted with Laemmli SDS Sample Buffer and heated at 95-100°C for 5 min followed by centrifugation at 16,000 x g at 4°C for 2 min ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-mouse or anti-rat with conjugated fluorochromes (Alexa Fluor 488, 568 or 647; ThermoFisher) at 1:500 ...
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bioRxiv - Developmental Biology 2020Quote: ... Alexa Fluor 488 goat anti-rat IgG (H + L, Thermo Fisher Scientific Cat# A-11006), CF488A goat anti-rat IgG (H + L ...
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bioRxiv - Biophysics 2021Quote: ... The membrane was then coated with Rat-tail collagen I (5 μg cm-2, Gibco) for 4 hours and rinses with PBS ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti-rat Alexa Fluor 555 and donkey anti-goat Alexa Fluor 488 (Life Technologies). Sections were counterstained by 5 min incubation in 300 µM DAPI intermediate solution (1:1,000 ...
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bioRxiv - Plant Biology 2019Quote: ... and then the corresponding secondary antibodies: anti-rat IgG (Alexa Fluor 488 conjugated, Molecular Probes A21210 and Dylight 550 Invitrogen SA5-10027 ...
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bioRxiv - Developmental Biology 2019Quote: ... Secondary antibodies were goat anti-rat Alexa Fluor 647 (Molecular Probes, A-21247, 1:500), goat-anti rat Alexa Fluor 555 (Molecular Probes ...
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bioRxiv - Microbiology 2019Quote: ... JAR4 binding was detected using FITC-conjugated rat anti-mouse IgG (H+L) (Thermo Fisher) at a 1:50 dilution for an incubation period of 1 h ...
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Differences in orthodenticle expression promote ommatidial size variation between Drosophila speciesbioRxiv - Evolutionary Biology 2021Quote: ... followed by anti-rat-Cy3 (Jackson Immuno Research) and anti-rabbit-Alexafluor 647 (Molecular probes) secondary AB staining ...