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Citations for Thermo Fisher :
3701 - 3750 of 10000+ citations for 7 Oxabicyclo 4.1.0 hepta 2 4 diene 1 3 4 trifluoro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... MEF cells were supplemented with CellEvent™ Caspase-3/7 green detection reagent (Life Technologies, Darmstadt, Germany) following manufacturer’s instructions before aldehyde fixation ...
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bioRxiv - Neuroscience 2023Quote: ... 30 microliters of the apoptosis detection reagent (CellEvent™ Caspase-3/7 Green Detection Reagent, Thermofisher, C10723) was added to each well and the slide returned to the incubator for another 60 minutes ...
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bioRxiv - Neuroscience 2023Quote: Dead and apoptotic cells were detected using the CellEvent Caspase-3/7 Kit (#C10423, Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2020Quote: ... 3) latrunculin-b (Lat-B; 2 μM; Fisher Scientific), 4 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mM 2-deoxy-D-glucose (2DG; ACROS Organics), 2 μM PERK inhibitor (PERKi ...
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bioRxiv - Plant Biology 2022Quote: ... 2–3 μg were treated with Turbo DNase (Ambion). RNA was circularized using T4 RNA ligase and reverse transcription was performed with the Superscript III (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... glass (2 gm, 3 mm bead diameter; Fisher Scientific) and polystyrene (24-well plates ...
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bioRxiv - Neuroscience 2022Quote: BDA was visualized with fluorophore-conjugated streptavidin (Thermo Fisher Scientific, Table 2; 1:1,000 for 3 h). The reaction was enhanced using the biotinylated tyramine (BT)-glucose oxidase (GO ...
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bioRxiv - Microbiology 2022Quote: Anti-ORF8 mAb (#1-3-2) was coupled to aldehyde/sulfate latex beads (Thermo Fisher Scientific A37304). The antibody was mixed with the latex beads and shaken at room temperature overnight ...
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bioRxiv - Neuroscience 2020Quote: ... was added prior to sonication on the lowest power setting (Output power: 3-4 Watts; 3x for 10 sec on, 30 sec off; Fisher Scientific Sonic Dismembrator Model 100). The samples were incubated with 150 units micrococcal nuclease (Cell Signaling ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge, Thermo Fisher Scientific, Darmstadt, Germany). The fluorescence intensity of the supernatant was measured measured (485 nm excitation ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were not allowed to reach confluency greater than 85% and were passaged every 3-4 days by dissociation into single-cell suspension using StemPro Accutase (Thermo Fisher Scientific, Waltham, MA, USA). When in single-cell suspension ...
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bioRxiv - Neuroscience 2019Quote: ... blocked for 30 min with 1% BSA in PBS-Tween (0.1%) and then incubated for 1 h with the primary antibodies (the lysosomal marker LAMP-1 (Invitrogen, 4-1071-82, 1:500) or the microglia marker IBA1 (Wako ...
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bioRxiv - Neuroscience 2023Quote: ... 1:200) at 4 °C 48 hours and in secondary antibodies simultaneously (Goat Anti-mouse 1:200, Invitrogen, Goat Anti-Rabbit 1:200, Jackson) at RT for 1 hour ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... using crude extracts from plant leaves and monitoring the quantity of 4-methylumbelliferone products generated from the substrate 4-methylumbelliferyl-β-D-glucuronide (Duchefa) on a fluorometer (Thermo Scientific fluoroskan ascent).
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bioRxiv - Cell Biology 2022Quote: ... primary antibody at a 1:500 dilution in 1% BSA for 90 min at room temperature or 24 hr at 4°C and was followed by incubation with Alexa Fluor 488 goat anti-rabbit secondary antibody (Invitrogen A11008; 4 μg/ml) in 0.2% BSA for 60 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... RNA quantity was measured using a Qubit 4 fluorometer (Qubit 4 fluorometer; Qubit dnDNA HS Assay Kit, Cat No. Q32854 Invitrogen, Eugene, OR, United States). RNA quality was assessed using an Agilent RNA Nano kit on a BioAnalyzer instrument (RNA 6000 Nano Kit ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from 4 young (⩽32 years) and 4 older patient (⩾40 years) granulosa cell samples using TRIzol (Life Technologies, Carlsbad, CA, USA) as previously described.111,112 Further ...
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bioRxiv - Neuroscience 2024Quote: ... Brains were postfixed in 4% paraformaldehyde overnight at 4 °C and cut into 50 µm sections on a cryostat microtome (Thermo Fisher, NX50, Waltham, MA). Mounted sections were imaged on a scanning confocal microscope (TCS SP5 ...
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bioRxiv - Neuroscience 2021Quote: ... in Tris-buffered PBS/ 0.1 % Tween 20 for 1 h at room temperature they were incubated overnight at 4 °C with the following antibodies: rabbit polyclonal anti-2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNPase; 49 kDa; 1:1000; Thermo Fisher Scientific, Waltham, MA, USA), mouse monoclonal anti-myelin-associated glycoprotein (MAG ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Cell Biology 2022Quote: ... HCEnCs were rinsed in DPBS and passaged at a ratio of 1:2 or 1:3 with TrypLE (Thermo Fisher Scientific) for 10-15 min at 37°C in 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... or 10 mM 2’,7’-dichlorodihydrofluorescein diacetate (DCFH-DA, Thermo Fisher Scientific, Waltham, MA, USA). The cells stained with TMRE or NAO were incubated for 15 minutes at 37◦C whereas cells stained with HE and DCFH-DA were incubated for 30 minutes at 37◦C in the dark ...
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bioRxiv - Immunology 2022Quote: ... The cells were incubated in presence of 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, 25 mM) (Invitrogen) for 30min at 37 °C and 5% of CO2 ...
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bioRxiv - Cancer Biology 2019Quote: ... 5(6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA; CA-DCF-DA; (C400, ThermoFisher Scientific)) at a stock concentration of 20 mM in DMSO was diluted in DMEM without phenol red to a concentration of 40 μM ...
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bioRxiv - Microbiology 2021Quote: ... was assessed by the cell-permeant 2′,7′-dichlorodihydrofluorescein diacetate (CFDA) (10 µg/ml, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were stained with 2 μM propidium iodide (PI) and 7 μM Hoechst 33342 (ThermoFisher) for 5-10 min and imaged under a Zeiss Observer Z1 microscope ...
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bioRxiv - Physiology 2023Quote: ... followed by 7 days of decalcification with Shandon’s TBD-2 (Thermo Scientific, Waltham, MA, USA) at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... SuperSignal Chemiluminescent substrate and 2’,7’-dichlorodihydrofluorescein diacetate acetyl ester (H2DCFDA) were from Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Cellular reactive oxygen species (ROS) levels were detected using 2’,7’ dichlorodihydrofluorescein diacetate (H2DCFDA; Invitrogen). Cells were treated in the absence or presence of drugs for 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... Immulon 4 HBX 96-well ELISA plates (Thermo Fisher Scientific) were coated with 2 μg/ml of recombinant trimeric S protein (50ul per well ...
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bioRxiv - Microbiology 2020Quote: ... Immulon 4 HBX 96-well ELISA plates (Thermo Fisher Scientific) were coated with 2 µg/ml of recombinant trimeric S protein (50 µL per well ...
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bioRxiv - Biophysics 2021Quote: ... The AutoScript 4 Software is commercially available from Thermo Fisher Scientific.
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bioRxiv - Biochemistry 2020Quote: SDS-PAGE electrophoresis (4-12% Bis-Tris gel; Life Technologies) demonstrating Ni-NTA purification of nsp10 and nsp16 ...
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bioRxiv - Cell Biology 2020Quote: ... target cells were selected using 4 μg/ml puromycin (ThermoFisher) or 150 μg/ml zeocin (Invivogen ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were separated by NuPAGE 4%–12%gradient gels (Invitrogen), transferred onto PVDF membranes (Immobilon®-P ...
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bioRxiv - Immunology 2021Quote: ... and were loaded on a 4-12% NuPAGE gel (Invitrogen). Electrophoresis was performed in an MES buffer at 200V for 35 min ...
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bioRxiv - Immunology 2021Quote: ... NK-92 cells were loaded with Fluo-4 NW (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Immunology 2021Quote: ... Right after centrifuging from the cytocentrifuge (Cytospin 4, Thermo Scientific), fresh BAL cell cytospin slides were stained using a Kwik-Dif Stains kit (Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... and run on 4-12% gradient Tris-glycine gels (Invitrogen). The proteins were transferred onto a nitrocellulose membrane (Thermo Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... 96-well flat bottom Immulon 4 HBX plates (ThermoFisher Scientific) were coated with 150 μl/well of fetuin (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were collected and fixed in 4% paraformaldehyde (Fisher Scientific) overnight before being processed through graded ethanols and xylene and then embedded in paraffin as previously described (Tavares et al. ...
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bioRxiv - Cell Biology 2022Quote: ... and resolved using NuPAGE 4-12% Bis-Tris gels (Invitrogen). Proteins were transferred to PVDF membranes and immunoblotted ...
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bioRxiv - Neuroscience 2021Quote: ... cryoprotected at 4°C in 30% sucrose (Thermo Fisher Scientific) for 48 h ...
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bioRxiv - Neuroscience 2021Quote: ... BMEs were labeled with FM 4-64 (Molecular Probes, Inc.) or by labeling RNA cargos by using the ExoGlow-RNATM EV Labeling Kit (System Biosciences ...
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bioRxiv - Molecular Biology 2020Quote: ... Hive Five cells (BTI-TN-5B1-4) (Thermo Fisher Scientific) were cultured in ESF921 medium (Expression Systems ...
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bioRxiv - Neuroscience 2021Quote: ... 4 ng/mL Alexa Fluor-647-conjugated azide (ThermoFisher, A10277), and 100 mM sodium ascorbate (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... and eluted by 4× lituium dodecyl sulfate (LDS; Invitrogen, NP0008). Membranes were immunoblotted with anti-V5 (ab15528) ...
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bioRxiv - Molecular Biology 2020Quote: ... quality and quantity were measured by Qubit 4 fluorometer (ThermoFisher).
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bioRxiv - Molecular Biology 2021Quote: ... run on 4-12% NuPAGE™ Bis-Tris gel (Invitrogen) and transferred on to Nitrocellulose membrane ...