Labshake search
Citations for Thermo Fisher :
3651 - 3700 of 10000+ citations for 7 Oxabicyclo 4.1.0 hepta 2 4 diene 1 3 4 trifluoro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2022Quote: ... in PBS prior to staining with DAPI (4=,6-diamidino-2-phenylindole) and mounted in Prolong Diamond antifade (Molecular Probes, Life Technologies). Images were captured using a Nikon Eclipse Ti confocal microscope (Nikon Instruments ...
-
bioRxiv - Microbiology 2023Quote: ... Fixed cells were stained with 4’,6-diamidino-2-phenylindole (DAPI) for visualization of host-cell nuclei and anti-MOMP antibody conjugated to FITC (Thermo Scientific™) for visualization of Chlamydia ...
-
bioRxiv - Genomics 2023Quote: ... nuclei were counterstained with ProLong Gold anti-fade reagent with 4’,6-diami-dino-2-phenylindole (DAPI) (Life Technologies, catalog no. P36935). Images were viewed on a Zeiss Axioskop 2 using AxioVision Rel ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were permeabilized and blocked for 1 h with DPBS containing 2% BSA and 0.1% Triton X-100 and then were incubated with 4 μg/mL anti-FLAG antibody diluted with 2% BSA in PBST with 0.1% Tween-20 (ThermoFisher Scientific, Cat# 85113) at 4 °C overnight ...
-
bioRxiv - Cancer Biology 2023Quote: ... Two hundred and fifty thousand NIH3T3 cells plated in a 6-well plate were transfected in the presence of Lipofectamine 2000 (Invitrogen) (per well 2 µg DNA/4 µl Lipofectamine 2000 in 400 µl Opti-MEM medium; Thermo Fisher Scientific). The next day ...
-
bioRxiv - Biochemistry 2023Quote: ... Grids were blotted for 2 s at 100% humidity at 4 °C and flash frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific).
-
bioRxiv - Bioengineering 2023Quote: ... The slides were thoroughly rinsed and sealed utilizing a SlowFade Gold antifade reagent with 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) and coverslips.44 The stained slides were imaged at 4x magnification with an EVOS fluorescence imaging system.
-
bioRxiv - Biophysics 2023Quote: ... 0.5% NP-40 alternative) with protease inhibitors and cleared for 2 hours at 4 °C with magnetic Protein G Dynabeads (Thermo Fisher, #10009D) before overnight immunoprecipitation with 1 ug per 250ug of proteins of either normal serum IgGs or specific antibodies as listed above ...
-
bioRxiv - Cancer Biology 2023Quote: ... were transfected with Lipofectamine 2000 (Invitrogen, Netherlands) (per well, 2 µg DNA/4 µl Lipofectamine 2000 in 400 µl Opti-MEM medium; Thermo Fisher Scientific). The next day ...
-
bioRxiv - Microbiology 2024Quote: ... The coupon was incubated in a 300 nM (100 ng/mL) solution of DAPI (4’,6-diamidino-2-phenylindole, ThermoFisher, Waltham, MA) for 3 minutes as per the manufacturer’s instructions ...
-
bioRxiv - Physiology 2024Quote: ... sections were incubated for 5 min with 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Dojindo, D523) and then mounted with PermaFluor (Thermo Fisher Scientific). Images were acquired using a BC43 or LSM800 instrument equipped with a Zeiss Axio Observer Z1 and a LSM 800 confocal unit with Airyscan module ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were co-stained with Draq5 (Cell Signal, 4048L) or nuclei stain 4’,6-Diamidine-2’-phenylindole dihydrochloride (DAPI; Thermo Fisher, D1306). Stained samples were imaged using an Olympus FV3000 Confocal Laser Scanning Microscope.
-
bioRxiv - Neuroscience 2024Quote: ... Cells were plated on laminin-coated 18 mm coverslips and treated with 2 μm Rhod2-AM and 4 μm Fluo4-AM (both from Thermo Fisher Scientific) for 15 min at 37°C in external solution (145 mM NaCl ...
-
bioRxiv - Biochemistry 2024Quote: ... 2 to 4 µL was injected onto an Acclaim PepMap 100 column packed with 2 cm of 5 µm C18 material (Thermo Fisher, 164564) using 0.1% formic acid in water (solvent A) ...
-
bioRxiv - Biochemistry 2024Quote: ... After selection for 2 weeks in DMEM supplemented with hygromycin B (250 μg/ml; Carl Roth) and blasticidin (4 μg/ml; Thermo Fisher Scientific), single-cell colonies were isolated ...
-
bioRxiv - Neuroscience 2021Quote: ... slices were fixed for 1 h in PBS with 4% PFA and Streptavidin conjugated to Alexa 647 (1:500, ThermoFisher Scientific) was applied during secondary incubation following blocking and permeabilization.
-
bioRxiv - Bioengineering 2019Quote: ... at 4°C followed by AlexaFluor-568 antibody (1:500) and concurrently with AlexaFluor-647-conjugated bungarotoxin (1:1000, Invitrogen, B35450) for two hours at room temperature ...
-
bioRxiv - Bioengineering 2019Quote: ... samples were incubated with mouse-anti-Pax7 (1:10, DSHB) overnight at 4°C followed by AlexaFluor-647 antibody (1:500, Invitrogen, A31573) for two hours at room temperature ...
-
bioRxiv - Bioengineering 2019Quote: ... NB100-2284) overnight at 4°C followed by AlexaFluor-568 antibody (1:500) or AlexaFluor-568 antibody (1:500, Invitrogen, A10087), respectively ...
-
bioRxiv - Immunology 2021Quote: 293T cells were co-transfected with pHDM-Wuhan-Hu-1 and pMAX-GFP plasmids at a 4:1 ratio by lipofectamine 3000 (Thermo fisher). 293T cell pool cotransfected with pHEF-VsVg and pMAX-GFP plasmids at a 4:1 ratio was used as a negative control ...
-
bioRxiv - Cell Biology 2020Quote: ... the pooled anion exchange fractions were phosphorylated in vitro overnight at 4 °C with 1 mM ATP and 1 μg of PKR(252-551)-GST enzyme (Thermo Scientific) per mg of eIF2α ...
-
bioRxiv - Immunology 2021Quote: ... 5×105 murine or human T cells have been stimulated at 1:1 ratio in 48well plate in presence of Sulfate Latex 4% w/v 5µm Beads (Molecular Probes S37227). For each experiment ...
-
bioRxiv - Microbiology 2021Quote: ... antibody 1:3000 in 1% PBS/BSA at 4°C followed by secondary antibody conjugated to Alexa Fluor 555 (Thermo Fisher) at 1:500 in PBS/BSA 1% was performed ...
-
bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibody (D1R: anti-D1R [mouse] 1:1500, Novus Biologicals, 48 hrs at 4°C; Th: anti-Th [rabbit] 1:1000, ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was brought up to 30 mL with Buffer K and nutated for 1 hr at 4°C with 1 mL bed volume of Buffer K-equilibrated His-Pur cobalt resin (Thermo Scientific), adding 30 uL fresh PI ...
-
bioRxiv - Microbiology 2021Quote: ... and incubated with primary antibodies (1% BSA/PBS-T, overnight at 4°C) at the following dilutions: mouse anti-SAG1 (1:1000, Thermo Scientific) and rat anti-HA (1:1000 ...
-
bioRxiv - Developmental Biology 2019Quote: ... Larvae were rinsed in PBST three times for 20 min and incubated overnight at 4°C with secondary antibodies (anti-mouse 1:500 IgG-Alexa Fluor 488, 1:500 IgG Alexa Fluor 594, Molecular Probes; anti-rabbit IgG-Alexa Fluor 488 ...
-
bioRxiv - Bioengineering 2019Quote: ... The respective spectra (average from 200 scans at 4 cm−1 resolution) were recorded between 4000-650 cm−1 using an FTIR Nicolet Nexus 470 instrument (Thermo Scientific), purged with dry ...
-
bioRxiv - Physiology 2020Quote: ... primary adipocytes were attached to poly-L-lysine (25-100 μg ml-1) coated coverslips and incubated with the Ca2+ fluorophore Fluo-4 AM (1 μM; Molecular Probes) in modified Hank’s solution for 1 hour at 21-23°C in the dark ...
-
bioRxiv - Cell Biology 2020Quote: ... The supernatant was removed and the RPE sheets/fragments were resuspended in 4 ml RPE media (1:1, DMEM3: Ham’s F12 medium (GIBCO 31330-038), 10% (v/v ...
-
bioRxiv - Neuroscience 2022Quote: ... Whole muscles were washed in 0.3% PBST for 5 hours then incubated in secondary antibodies for 48h at 4°C on rotating incubator (AlexaFluor 568, 1:200, Invitrogen, A11041; AlexaFluor 647, 1:200, Invitrogen, A21236). Whole muscles were dehydrated in a series of 25% ...
-
bioRxiv - Cancer Biology 2022Quote: ... at 1:250 dilution overnight at 4°C and anti-mouse AlexaFluor-555 secondary antibody (1:1000, ThermoFisher Scientific, #A-21424) for 1 hour at room temperature ...
-
bioRxiv - Developmental Biology 2024Quote: ... and incubated at 4°C for 18 hours with primary antibodies: E-Cad (13-1700, Thermo Fisher, HECD-1, 1:100) and CK-14 (RB-9020 ...
-
bioRxiv - Cell Biology 2024Quote: ... 1:600) antibody was incubated at 4°C overnight followed by secondary antibody anti-rabbit Alexa 488 (ThermoFisher Scientific, 1:600) for 1h at RT ...
-
bioRxiv - Cell Biology 2023Quote: ... Frozen rat ovarian follicles were homogenized in 400 µL lysis buffer (100 mM HEPES, pH 8, 4% SDS, 1 mM EDTA, and 1× Halt protease/phosphatase inhibitor cocktail (ThermoFisher Scientific) using Zirconia/glass beads in a bead beating grinder (FastPrep24 ...
-
bioRxiv - Immunology 2023Quote: ... 20 ng ml-1 GM-CSF (Biozym) or X38-Ag8 supernatant (1%) and from day 5 on additionally with 10 ng ml-1 IL-4 (Life technologies). At day 7 ...
-
bioRxiv - Biophysics 2023Quote: ... blocked and imaged as described under section triple-color immunostainings but were incubated with mouse anti-AnkG (#AB_10675130, Neuromab, 1:200) overnight at 4°C and subsequently with donkey anti-mouse AF647 (#A31571, Invitrogen, 1:800) for 1h.
-
bioRxiv - Molecular Biology 2023Quote: ... or anti-NIPBL rabbit antibodies followed by 1 h incubation at 4 °C on a rotating wheel with 40 μl of a 1:1 mix of Dynabead protein A (10001D, Life Technologies) and G (10003D ...
-
bioRxiv - Cell Biology 2022Quote: ... The cells were washed and suspended with 1 mL of 4% FBS/PBS containing DAPI (1:2000; Thermo Fisher Scientific, 62248) and DRAQ5 (1:5000 ...
-
bioRxiv - Biochemistry 2023Quote: ... containing 1 mM EDTA and 1 mM dithiothreitol were reacted with a 4-fold concentration of Protein Thermal Shift Dye (Life Technologies) in a 20 μl reaction volume ...
-
bioRxiv - Neuroscience 2023Quote: ... 4) with secondary Abs anti-mouse or rabbit IgG conjugated Alexa 488 (1:400) or Alexa 568 (1:200) (Life Technologies).
-
bioRxiv - Genomics 2023Quote: ... plugs were washed 4 times in 1×Wash Buffer (BNG) and 5 times in 1× TE Buffer (ThermoFisher Scientific, Waltham, MA). Then ...
-
bioRxiv - Immunology 2023Quote: NK cells were activated by co-culturing NK cells with irradiated K562 cells expressing membrane bound interleukin-21 and 4-1BBL at 1:1 ratio in RPMI (Gibco, Thermo Fisher) supplemented with 10% FCS (Sigma-Aldrich) ...
-
bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed 1: 1 with SDS loading buffer and loaded on a NuPageTM 4-12% Bis-Tris PAGE Gel (Invitrogen™) along with Novex™ Sharp Pre-stained Protein Standards (range from 3.5 to 260 kDa ...
-
bioRxiv - Microbiology 2019Quote: ... Caspase-like activity was measure with CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (Invitrogen), a nucleic acid-binding dye that harbors the caspase-3/7 cleavage sequence ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then resuspended in 10 µM CellEvent Caspase 3/7 activity reporter in PBS (Invitrogen, #C10723) and incubated for 30 minutes at 37 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were passaged every 3-7 days as single cells using TrypLE™ Select CTS™ (Gibco). The ROCK inhibitor Y27632 (Fujifilm ...
-
bioRxiv - Microbiology 2020Quote: ... For detection of effector caspase activity 20 µM CellEvent Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific) was added to the infected cells prior to imaging ...
-
bioRxiv - Cancer Biology 2019Quote: ... Taqman® assays (Supplementary table 3) and QuantStudio™ 7 Flex Real Time PCR system (ThermoFisher, USA), and relative expression levels determined using QuantStudio™ 7 Real Time PCR software.
-
bioRxiv - Neuroscience 2019Quote: ... unc-7 rescue plasmids were made using the Multisite Gateway® 3-Fragment Vector Construction Kit (Invitrogen). A 1078bp mec-4 promoter fragment (as previously used ...