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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for 7 Oxabicyclo 4.1.0 hepta 2 4 diene 1 3 4 trifluoro 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and incubated with primary antibodies overnight at 4 °C: pSTAT1-Tyr701 (ThermoFisher Scientific MA515071, 1:1000 dilution), STAT1 (Santa Cruz Biotechnology SC-417 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were probed with an SLC35A2 polyclonal antibody overnight at 4°C (1:2000, Invitrogen, PA5-77178), followed by incubation with goat anti-rabbit HRP-linked secondary antibody (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then stained using primary antibodies overnight at 4°C: tubulin (Invitrogen MA1-80017, 1:1000), aSyn (BD Transduction Laboratories 610786 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated at 4°C for 30 min with antibodies against CD45 (Invitrogen, #MCD4528, 1/25), CD31 (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... immortalized FSHD myoblasts were cultured in LHCN medium (4:1 DMEM:Medium 199 (ThermoFisher Scientific, Waltham, MA, USA) supplemented with 15% fetal bovine serum (FBS ...
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bioRxiv - Biophysics 2022Quote: ... pH 8.0 supplemented with 1 mM phenylmethylsulfonyl fluoride (PMSF) and 4 Pierce protease inhibitor tablets (Thermo scientific), then stirred gently on a Variomag magnetic stirrer MONO DIRECT (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... 4 μl of 1 M CaCl2 and 5 μl of 100 U MNase (88216, Thermo Fisher Scientific) were added ...
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bioRxiv - Genetics 2023Quote: ... 1/10 of each eluate was resolved on a 4–12% NuPage Novex gel (Thermo Fisher Scientific) and stained with SilverQuest staining kit (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... for 1 hour at 4°C and then was loaded onto Pierce™ Glutathione Agarose (16101; Thermofisher) by gravity flow ...
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bioRxiv - Genomics 2023Quote: ... TaqMan qPCR reactions contained: 4× TaqPath 1-Step RT-qPCR multiplex reaction master mix (ThermoFisher, Cat# A28521), 4pmol of each primer ...
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bioRxiv - Molecular Biology 2023Quote: ... washed (4 x 300 µL PBST) and blocked for 1h at RT with 1% BSA (Fisher Scientific) in PBS solution ...
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bioRxiv - Bioengineering 2023Quote: ... constructs were fixed with 4% paraformaldehyde and washed with PBS and stained for LipidTox (Invitrogen 1:500) and DAPI (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... TaqMan qPCR reactions contained: 4× TaqPath 1-Step RT-qPCR multiplex reaction master mix (ThermoFisher, Cat# A28521), 4pmol of each primer ...
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bioRxiv - Cell Biology 2023Quote: ... for 1 hour at 4°C and then was loaded onto Pierce™ Glutathione Agarose (16101; Thermofisher) by gravity flow ...
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bioRxiv - Cancer Biology 2024Quote: ... Glut-1 and catalase proteins were separated on a 4% to 20% Tris-Glycine gel (Life Technologies) by SDS-PAGE and transferred to a nitrocellulose membrane ...
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bioRxiv - Immunology 2020Quote: ... Staining was performed on ice for 25 minutes in PBS with 2 % FCS using the following antibodies: 7- AAD 1:400 (Thermo Fisher Scientific), CD19-BV786 1:20 (clone SJ25C1 ...
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bioRxiv - Bioengineering 2022Quote: ... Cultures were washed three times with washing solution and then incubated for 30 min at room temperature on the OrganoFlow (7°, 2-min intervals) with Alexa Fluor 488 (#a32731; Invitrogen; 1:250) and Alexa Fluor 647 (#a31571 ...
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bioRxiv - Genetics 2021Quote: ... the 700 bp PCR products were subcloned into the pCR™4-TOPO® TA vector and transfected into Escherichia coli DH5α cells (TOPO 4 Kit, ThermoFisher Scientific) followed by plasmid preparation and direct Sanger sequencing ...
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bioRxiv - Immunology 2021Quote: ... pre-stained cells were loaded with Fluo-4 via by mixing with equal volumes of 2X Fluo-4 Direct™ loading solution (Fluo-4 Direct™ Calcium Assay Kit, Thermo Fisher). After a 30 min incubation at 37°C and then 30 mins at RT ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were quickly fixed overnight (ON) in 1x PBS pH7.4, 4% sucrose (Serva, Heidelberg, Germany) and 4% PFA (Paraformaldehyde; Thermo Fisher Scientific, Waltham, USA). Fixed brain slices were washed once with 1x PBS pH 7.4 and subsequently submerged in 30% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... cells were centrifuged (300rcf, 7min, 4°C) and fixed (1h, 4°C) using the eBioscience Foxp3/Transcription factor staining buffer set (ThermoFisher #00-5523-00) fixation/permeabilization reagent ...
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bioRxiv - Neuroscience 2024Quote: ... 30 μg of protein per lane was loaded and run on a 4–12% Bis-Tris PAGE gel (Bolt 4–12% Bis-Tris Protein Gel, Invitrogen, catalogue no. NW04122BOX) and transferred onto a polyvinyl difluoride membrane (Immobilon-FL ...
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bioRxiv - Bioengineering 2023Quote: ... The staining of MyHC was performed by incubating the samples for 4 hours with the mouse anti-MyHC antibody (Myosin 4, eFluor™ 660, Clone: MF20, Affymetrix eBioscience™), which was used at a 1:10 dilution for a final concentration of 10 μg/mL ...
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bioRxiv - Biophysics 2022Quote: ... The grids were automatically blotted for 4 s at 4°C and 100% humidity and flash-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific, USA). Cryo-EM data were collected using a 300 kV Titan Krios G2 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... A droplet of 2.5 μL of purified NaV1.6EM complex was applied to the grid followed by blotting for 4-5s at 4°C under 100% humidity using a Vitrobot Mark IV (Thermo Fisher Scientific, USA). In the case of the preparation of NaV1.6EM complex with 4,9-anhydro-TTX ...
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bioRxiv - Biophysics 2023Quote: ... cells were transfected with 4 μg of plasmid per 4 million cells using the lipofectamine 2000 reagent and protocol (Thermo Fisher Cat# 11668019). Nuclear extraction was performed 24 hours after transfection utilizing nuclear extraction kit from Abcam (ab113474) ...
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bioRxiv - Cell Biology 2024Quote: ... fixed in 4% PFA for 15 minutes at 4 °C and incubated in 1X InVitrogen eBioscience Cell Permeabilization buffer (InVitrogen 00-8333-56) for 15 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... and coverslips were washed with 1X PBS and fixed with cold 4% PFA/PBS pH 7.4 (Paraformaldehyde Solution, 4% in PBS, Thermo Scientific™, #J19943.K2) for 15 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... and 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) was used for DNA staining ...
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bioRxiv - Biophysics 2024Quote: ... and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydro (EDC, ThermoFisher). For BS3 crosslinking ...
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bioRxiv - Immunology 2022Quote: Caspase-3 activity was determined using EnzChek™ Caspase-3 Assay Kit #2 (Thermo Fisher).
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bioRxiv - Microbiology 2021Quote: ... (75 mm i.d. 3 2 cm, Acclaim PepMap100 C18 3 mm, 100 A°, ThermoFisher Scientific) and separated over an EASY-Spray column ...
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bioRxiv - Genetics 2021Quote: ... at 85 V for 2 hours 15 minutes at an ambient temperature of 4 °C in NuPAGE™ Transfer Buffer (Invitrogen, UK) containing 10% 2-propanol (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... containing 4′,6-diamidino- 2-phenylindole (DAPI) and were transferred onto microscope slides with large-orifice 200 µL-tips (Fisher Scientific, 02707134). The following antibodies or reagents were used ...
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bioRxiv - Neuroscience 2021Quote: ... 20 µl of either type of samples were run on BOLT 4-12% Bis-Tris mini protein gels for SDS-PAGE and electroblotted using the iBlot 2 dry blotting system (Thermo Fisher, USA). Polyvinylidene fluoride membranes were probed with anti-PrP monoclonal antibody ICSM-18 (1:4,000 ...
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bioRxiv - Cell Biology 2019Quote: ... staining was performed in three-dimensional cysts with 2 mM calcein AM and 4 mM ethidium homodimer I (Live/Dead® Viability/Cytotoxicity kit; Life Technologies) for 40 min at 37°C in the dark ...
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bioRxiv - Cancer Biology 2019Quote: Cells were air-dried and fixed in 4% paraformaldehyde in phosphate-buffered saline (PBS) for 20 minutes on glass slides using Shandon cytospin 2 (THERMO FISHER SCIENTIFIC). Fixed cells were permeabilized ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein samples were heat-denatured at 98°C for 8 minutes in denaturing Laemmli buffer (containing 2-mercaptoethanol) and resolved on 4–12% gradient Invitrogen Bolt Bis-Tris Plus gels (Thermo Fisher Scientific). The resolved proteins were transferred via wet transfer in buffer (1X Tris-glycine ...
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bioRxiv - Cancer Biology 2019Quote: ... The molecular sizes of peak 1 (300 kDa) and peak 2 (150 kDa) were determined by SDS gel electrophoresis (NuPAGE 4-12% Bis-Tris Gel; Life Technologies, CA) under non-reducing conditions along with antibody standards.
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bioRxiv - Neuroscience 2019Quote: ... All the fluorescent indicators employed in the study (fura-2 AM, fluo-4 AM, hydroethidine, TMRE, and MitotrackerGreen FM) were obtained from Molecular Probes (ThermoFisher). Unless otherwise specified ...
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bioRxiv - Neuroscience 2020Quote: ... Embryo hippocampi were dissected in 4 °C Hibernate E supplemented with 2% B27 supplements and 100 U/mL Penicillin/Strep (Thermo Fisher Scientific). Hippocampal tissues were digested in Hibernate E containing 20 U/mL papain ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Cancer Biology 2019Quote: ... washed extensively with phenol red-free RPMI containing 2% FBS and stored overnight at 4°C prior to staining with CellEvent Senescence Green (Thermo Fisher Scientific). Flow cytometric analysis and sorting was performed at the Flow Cytometry shared Resource Facility at the Icahn School of Medicine at Mount Sinai ...
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bioRxiv - Biochemistry 2021Quote: ... Grids were then blotted with filter paper for 2 s at 100 % humidity at 4 °C and frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... cells were washed three times with PBS and mounted on the slide with ProLong Diamond antifade with 4′,6-diamidino-2-phenylindole (DAPI; Life Technologies, #P36966). Confocal images were acquired on a Nikon Ti-Eclipse A1M microscope fitted with a 60× oil immersion objective using 488 nm ...
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bioRxiv - Microbiology 2020Quote: ... formic acid and the peptides equivalent to 2-4 µg of proteins were loaded for mass spectrometry analysis on a Orbitrap Elite mass spectrometer (ThermoFisher Scientific Inc.). The separation of peptides was performed on an analytical column (75 µm × 15 cm ...
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bioRxiv - Molecular Biology 2020Quote: ... Ten-microliter sample were loaded at a 0.45 mL/minute flowrate onto an anion separator (2 × 250 mm, 4 μm, AS19, Thermo Scientific, Bremen, Germany) with a 50 mm guard with the same separator material ...
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bioRxiv - Cancer Biology 2020Quote: 10 ul whole cell extracts of 2 × 105 cells in 40 μl 6X SDS loading buffer were run on 4-14% bis-tris gel (Invitrogen cat # NP0335). Membranes were transferred by semi-dry apparatus (Bio-Rad Transblot cat # 170-3940 ...
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bioRxiv - Biophysics 2020Quote: ... The grids were blotted for 4 s or 2 s and rapidly cryocooled in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific) at 4°C and 100% humidity ...
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bioRxiv - Cell Biology 2021Quote: ... cells were loaded with the Ca2+ indicator Fluo-4/AM (for intracellular Ca2+) or Rhod-2/AM (for mitochondrial Ca2+) (Thermo Fisher Scientific) for 15 min at 37°C ...