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Citations for Thermo Fisher :
3551 - 3600 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... First-strand cDNA synthesis was performed on 11 μl of total RNA using 10 pmol of each primer in a 20ul total reaction (SuperScript III, Thermo Fisher) using the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Ly6A/E(Sca-1)-PE (Clone D7, cat. # 12-5981-82), CD16/CD32-FITC (clone 93, cat. # 11-0161-82) were purchases from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The concentration of the Cas7-11–Csx29–crRNA complex was measured using Pierce 660-nm Protein Assay Reagent (Thermo Fisher Scientific). Csx30 was expressed in E ...
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bioRxiv - Molecular Biology 2022Quote: ... using sterile surgical blade (Size: 11) by immobilizing the insect on ice and collected antennae into 1.5mL Eppendorf tube containing RNAlater® solution (Thermofisher, USA). Fifty pairs of weevil antennae were used per replicate ...
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bioRxiv - Immunology 2022Quote: ... RNA was eluted from the magnetic beads with 11 μL of a solution containing 14.5 ng/μL of random primers (Invitrogen, Cat # 48190011), 0.5% of Igepal Ca-630 (type NP-40 ...
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bioRxiv - Microbiology 2022Quote: ... 1mM DTT, 300 mM KCl, 20µM biotin-11-ATP, -UTP, -GTP, -CTP, 0.4 U/ml RNase inhibitor [RNaseOUT ThermoFisher] and 1% Sarkosyl) was added to thawed nuclei ...
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bioRxiv - Cell Biology 2024Quote: ... and reverse transfected with 1 pmol/well of a combination of 3 non-overlapping 11-mer siRNAs (Ambion Silencer® Select, Thermofisher) using Lipofectamine RNAiMax transfection reagent (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... CD3 FITC (clone OKT3, catalog #: 11-0037-42) and CD66b PE-Cy7 (clone G10F5, catalog #: 25-0666-42) from Thermo Scientific; CD56 FITC (clone MEM-188 ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were collected by centrifugation and labelled using tandem mass tag (TMT) 11-plex isobaric mass tagging reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The 14.4.4 mAb and the I-Ak-reactive 11-5.2 mAb were randomly labeled via available lysine residues with Alexa Fluor 647 carboxylic acid succinimidyl ester (Thermo Fisher Scientific) according to the manufacturer’s instructions and purified via gel filtration (Superdex-200 10/300 GL ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with either scrambled (non-targeting #1) siRNA or Septin9 siRNA (J-048947-11-0050 ONTARGETplus) with Lipofectamine-iMAX (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were harvested for up to 11 days and the cell number recorded using a Countess IIR automated cell counter (Life Technologies).
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bioRxiv - Cell Biology 2023Quote: ... we washed and rested islets overnight in islet medium (RPMI 1640 with 11 mM glucose, 10% FBS, and 1% Penicillin-Streptomycin (Gibco, #15140122)) ...
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bioRxiv - Microbiology 2023Quote: ... For FcγRII we stained with anti-CD32-FITC (Cat# 60012.FI, StemCell) and corresponding mouse IgG2b-FITC isotype control (Cat# 11-4732-81, ThermoFisher Scientific). For FcαRI we stained with anti-CD89/-PE (cat# 555686 ...
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bioRxiv - Molecular Biology 2023Quote: The surface of all cultureware used for the maintenance and propagation of human pluripotent stem cells (WTC-11 line) was coated with Corning Matrigel (Fisher Scientific) prior to cell seeding ...
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bioRxiv - Molecular Biology 2023Quote: Differentiated EB cells were desegregated and stained with the anti-mouse CD71-APC antibody (eBioscience, 11-0711-80) (1:8000 in staining buffer) and Hoechst (Invitrogen, 33258) (1:10000 in staining buffer ...
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bioRxiv - Systems Biology 2023Quote: We conducted mass spectrometry analysis of 120 CSF samples using 13 batches of 11-plex tandem mass tag (TMT, Thermo Scientific) experiments ...
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bioRxiv - Bioengineering 2024Quote: ... which was monitored using a Traceable™ Jumbo Thermo-Humidity Meter (cat. #11-661-19, Fisher Scientific, Saint-Laurent, Quebec, Canada).
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bioRxiv - Microbiology 2024Quote: ... and resuspended in 50 μL anti-CD32-FITC (Cat # 60012.FI, StemCell) or isotype control (Cat # 11-4732-81, ThermoFisher Scientific), and incubated at 4°C for 20 min ...
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bioRxiv - Genomics 2024Quote: ... The muscle dissociation buffer contained collagenase II (700-800 U/mL) and dispase (11 U/mL) in Ham’s F-10 nutrient mixture with 1 mM l-glutamate (ThermoFisher Scientific, 11550043) supplemented with 10% horse inactivated serum (HS ...
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bioRxiv - Biochemistry 2022Quote: ... the plate was developed using 50 µL of 1-StepTM Turbo-TMB-ELISA Substrate Solution (ThermoFisher) per well and the plates were quenched with 50 µL of 2M H2SO4 to each well ...
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bioRxiv - Immunology 2021Quote: ... The plates were developed with 1-Step Ultra TMB-ELISA Substrate Solution (34028, Thermo Fisher Scientific) and the reaction was stopped by adding with 2 N sulfuric acid (BDH7500-1).
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bioRxiv - Cancer Biology 2019Quote: ... the concentration of Interferon-γ (IFNγ) in the media was measured by ELISA (Invitrogen, 88-7314). The T cells from 24-hour co-cultures were harvested ...
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bioRxiv - Immunology 2021Quote: ... The colorimetric reaction was terminated by the addition of 100 μL of ELISA Stop Solution (Invitrogen). The absorbance at 450 nm and 650 nm was read by spectrophotometry using a SpectraMax iD3 microplate reader (Molecular Devices).
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bioRxiv - Immunology 2019Quote: ... the concentration of IFN-γ in the cell culture supernatant was determined by ELISA (Invitrogen, #EHIFNG2). An IFN-γ standard was included in the ELISA and linear curve fitting using the GraphPad Prism 7.0 a software was used to derive the IFN-γ concentration in the samples from the absorbance at 450 nm (A450 ...
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bioRxiv - Cancer Biology 2020Quote: ... O.D values were determined at a wavelength of 590 nm using an ELISA reader (Thermo Scientific) The percentage survival was determined as the ratio of treated cells versus vehicle control after background subtraction ...
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bioRxiv - Microbiology 2021Quote: ... pH 9.6] was used to coat wells of an uncoated 96-well ELISA plate (Nunc Maxisorp) at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ... The assay reaction was developed with the 1-step Ultra TMB-ELISA substrate (Thermo Fisher Scientific). The reaction was left to develop for 5 min and then stopped with 2M H2SO4 ...
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bioRxiv - Microbiology 2022Quote: ... Supplementary Table 3) and added (100 μL/well) to ELISA plates (Maxisorp Nunc-immuno plates; Thermo). Since Iwate/2019(H3N2 ...
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bioRxiv - Biochemistry 2021Quote: ... Background binding level in ELISA assays was measured using Human IgG Isotype Control (ThermoFisher #02-7102).
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bioRxiv - Immunology 2020Quote: ... plasma was serially diluted (4-fold dilutions) and added to ELISA plates (Maxisorp, Nunc, MA USA) pre-coated with 10μg/ml OVA (Sigma Grade V ...
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bioRxiv - Immunology 2019Quote: ... plasma was serially diluted (4-fold dilutions) and added to ELISA plates (Maxisorp, Nunc – MA, USA) pre-coated with 10µg/mL OVA (Sigma Grade V ...
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bioRxiv - Bioengineering 2021Quote: ... The signal was developed with 1-Step™ Ultra TMB-ELISA substrate solution (Thermo Fisher Scientific) and the reaction was stopped with 1% HCl ...
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bioRxiv - Neuroscience 2020Quote: ... Pierce HRP ECL substrate was used to detect luminescence in ELISA (Thermo Fisher Scientific, USA, #32209). Tris-buffered saline with Tween-20 (TBST ...
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bioRxiv - Immunology 2019Quote: ... Supernatants were harvested and assayed for IL-8 by ELISA (Thermo Fisher Scientific #88-8086-77). Alternatively ...
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bioRxiv - Immunology 2022Quote: ... Colorimetric detection was accomplished using 1-Step™ Ultra TMB-ELISA Substrate Solution (ThermoFisher Scientific: 34029) and color development was quenched with 1.0 N HCl ...
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bioRxiv - Immunology 2022Quote: ... 25 μL/well of 1-step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, cat. # PI34029) was added to the plates and incubated for 5 to 10 min at RT ...
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bioRxiv - Immunology 2022Quote: ... The color reaction was developed using the 1-StepTM Ultra TMB-ELISA substrate solution (Thermo Scientific) and plates were read at 450nm using the Labtech LT 4500 microplate reader ...
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bioRxiv - Microbiology 2022Quote: ... followed by subsequent addition of Turbo 3,3′,5,5′ tetramethylbenzidine (TMB)-ELISA substrate solution (Thermo Fisher Scientific) and 2M sulfuric acid stop solution ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were used to perform ELISA to quantify the levels of IL-1β (Thermo Fisher Scientific), IL-6 (Biolegend) ...
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bioRxiv - Neuroscience 2022Quote: ... the plates were incubated for 30 minutes with Pierce 1-Step Ultra TMB ELISA Substrate (ThermoFisher). Pre-warmed sulfuric acid stop solution (DY994 ...
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bioRxiv - Neuroscience 2022Quote: ... the plates were incubated for 30 minutes with Pierce 1-Step Ultra TMB ELISA Substrate (ThermoFisher). Pre warmed sulfuric acid stop solution (DY994 ...
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bioRxiv - Immunology 2022Quote: ... TNF concentrations in the supernatants were measured by ELISA (Fisher Scientific, Waltham, MA, Cat# 88-7346).
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bioRxiv - Pathology 2024Quote: ... The total amount of TGFβ1 was determined on technical duplicates by ELISA (88-8350-22, Invitrogen), after heat denaturation followed by acidic treatment and neutralization according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were washed and then developed by adding Slow Step ELISA TMB substrate (Thermofisher, Cat: 34024) to each well ...
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bioRxiv - Biochemistry 2024Quote: ... Signal was developed by treating cells with 200 μl TMB-ELISA (Thermo Scientific, Cat no. 34028) until the light blue colour appeared ...
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bioRxiv - Immunology 2024Quote: ... the plates were washed with TBS-T and SuperSignal™ ELISA Femto Maximum Sensitivity Substrate (ThermoFisher) was added following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... 100 μl horseradish peroxidase substrate (1-Step™ Ultra TMB-ELISA Substrate Solution) (Thermo Scientific™) was added into wells followed by 100 ul 1M H2SO4 ...
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bioRxiv - Cell Biology 2024Quote: ... Supernatants were harvested for IL-1β measurement using a specific ELISA (Thermo Fisher, 88-7621-22). Apoptotic cells were generated by retroviral transduction or MTX treatment during 20-24 h when cell death approaches its maximum ...
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bioRxiv - Cell Biology 2023Quote: ... 10μg purified FNBP4 was coated into the wells (maxisorp surface) of ELISA plates (#439454 Nunc Maxisorp) along with 1X PBS for the control experiment and allowed to incubate overnight at 4 °C ...