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Citations for Thermo Fisher :
3601 - 3650 of 9363 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... for 3-4 days to OD 0.6 in tryptone soya broth (Oxoid) supplemented with 10% FCS (Gibco) and the antibiotics above ...
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bioRxiv - Microbiology 2024Quote: ... Cells were maintained with Dulbecco’s Modified Eagle’s medium (DMEM) supplemented with 10% fetal calf serum (FCS) (Gibco) and maintained at 37°C with 5% CO2.
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bioRxiv - Cancer Biology 2019Quote: ∼1 x 106 HEK293 cells grown at 70% confluency on 35 mm dish embedded with 12 mm glass viewing area (Nunc, Thermo Fisher Scientific Inc., Waltham, MA, USA) were transfected with appropriate plasmids using Lipofectamine 3000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The T cells were isolated using the EasySep human T cell isolation kit (Stemcell) and activated with human T-activator CD3/CD28 Dynabeads (Gibco) at a bead:cell ratio of 1:1 ...
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bioRxiv - Molecular Biology 2022Quote: In vitro sumoylation reactions were performed on protein array slides with more than 9000 human proteins spotted (ProtoArray® Human Protein Microarray v5.0, Invitrogen). The reaction was performed by the technical service of Invitrogen with our purified enzymes ...
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bioRxiv - Immunology 2022Quote: ... For Cytokine Multiplex of Human Inflammation Panel (Invitrogen™ Inflammation 20-Plex Human ProcartaPlex™ Panel) (Catalog # EXP20012185901, eBioscience/Invitrogen) procedures were performed following manufacturer protocol and the plate was processed on BioRad Bio-Plex 200 system with Bio-Plex-HTF attachment (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: RNA was isolated from human NSCLC cells and human clinical NSCLC tumor and normal samples by homogenization in Trizol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... supernatants were diluted 4-fold and assayed with the Amyloid beta 40 Human ELISA Kit and either the Amyloid beta 42 Human ELISA Kit or the Amyloid beta 42 Human ELISA Kit Ultrasensitive (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: HMF and MRC5 fibroblasts were transfected with a pool of 3 siRNAs targeting each of the 710 human kinases (Silencer Human Kinase siRNA Library, #A30079 Thermofisher). Fibroblasts (1250 cells/well ...
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bioRxiv - Immunology 2019Quote: ... human PBMC derived T cells were purified by Human T cell isolation kit from Invitrogen (Invitrogen Dynal AS, Oslo, Norway) according to manufacturer’s instruction ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were rinsed and re-suspended in flow buffer containing 25 μL mL−1 of each of the following monoclonal anti-human antibodies per sample: anti-human CD3-AF700 (56-0037-42, ThermoFisher), - CD40L/CD154-FITC (11-1548-42 ...
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bioRxiv - Immunology 2021Quote: ... and total T cells were purified from PBMC suspensions by negative immunomagnetic selection (purity >90%) using the Human Myeloid DC Enrichment Kit (STEMCELL) and the Untouched total human T cell (Invitrogen) kits ...
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bioRxiv - Cancer Biology 2022Quote: ... primary human breast CAFs were transfected with 75 nm human periostin-targeting stealth siRNA (si-POSTN) (ThermoFisher siRNA ID: HSS116400) or 75 nm non-targeting control siRNA (si-Control ...
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bioRxiv - Cell Biology 2022Quote: ... were prepared from fresh human umbilical veins as previously described (Barbieri et al., 1981) and maintained in Human Endothelial SFM (Gibco™ ...
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bioRxiv - Microbiology 2022Quote: ... Surface PCDH1 was stained using human anti-EC7 mAb-3677 (5 µg/mL) followed by anti-human Alexa FluorTM 555 (ThermoFisher) for 1 h at 4°C each ...
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bioRxiv - Microbiology 2022Quote: ... encoding full-length human MCT1 (uniprot ID: P53985) and human basigin (uniprot ID: P35613-2) were cloned individually into pFastBac vector (Invitrogen) for recombinant expression in Sf9 cells ...
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bioRxiv - Molecular Biology 2023Quote: ... human ETAR (residues 21-406) and human ETBR (residues 27-424) DNA was cloned into a modified pFastBac vector (Invitrogen), which contains an N-terminal FLAG tag (DYKDDDD ...
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bioRxiv - Neuroscience 2023Quote: ... The human FUS or human TARDBP cDNA was subcloned into the pENTR™/D-TOPO® vector (Thermo Fisher Scientific). To generate the Gateway destination vector pUAST-DEST ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Cell Biology 2023Quote: Human islets were transfected with 60 nM siRNA targeting human CEBPG (s2901, Silencer Select Pre-designed siRNA, Ambion, Life Technologies), TMEM176A (s226845 ...
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bioRxiv - Neuroscience 2023Quote: ... and secreted signaling proteins were measured in culture supernatants by the Human Immune Monitoring Center (HIMC) at Stanford University using a Human 48-plex Luminex Procarta Immuno-assay (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: Human colorectal adenocarcinoma (ATCC, #SK809) and human pancreatic carcinoma (ATCC, #CRL1469) cells were grown in McCoy’s 5A medium (Gibco, #16600082) and Dulbecco’s Modified Eagle’s medium (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... Adult human dermal fibroblast (HDFa) cells (Cascade Biologics C-013-5C) were maintained in Human Fibroblast Expansion Basal Medium (Gibco) supplemented with Low Serum Growth Supplement (Gibco).
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bioRxiv - Cell Biology 2021Quote: ... the cell number was determined by manually counting of 10×13 tile images (4X) using Fiji Software.27 Cells were harvested using TrypLE™ Express Enzyme (Gibco, Thermo Fisher Scientific) and subcultured in the same conditions ...
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bioRxiv - Microbiology 2020Quote: The flow-through fraction of each TMT11 experiment was fractionated into 13 fractions using the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2020Quote: ... Both genomic DNA and cDNAs were analyzed on DT96 real-time DNA amplifier (DNA-Technology) using SYTO™ 13 Green Fluorescent Nucleic Acid Stain (Thermo Fisher Scientific). RT-qPCR values were normalized to the rp49 mRNA and genomic DNA qPCR values were normalized to rp49 genomic qPCR ...
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bioRxiv - Cell Biology 2019Quote: ... ~1 μg purified RNA was used to generate cDNA via High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Cat. No. 43-688-13). qRT-PCR was performed with TaqMan Universal Master Mix II ...
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bioRxiv - Molecular Biology 2020Quote: ... all samples were purified using AmpureXP beads at a volumetric ratio of 0.5X with a final elution in 13 µl of H2O (DNase/RNase-Free Distilled H2O, Thermo Fisher Scientific #10977-049). The cDNA was then quantified using the Qubit High Sensitivity dsDNA Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... As a control, siGENOME Non-Targeting Pool #1 (Dharmacon, D-001206-13-05) and a second independent predesigned PURA siRNA (Thermo Fisher Scientific, 289567) was used as described for the PURA siRNA pool ...
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A rare variant on a common risk haplotype of HFE causes increased risk of hereditary hemochromatosisbioRxiv - Genetics 2019Quote: ... then Western blots were performed using the anti-GFP antibody (1:2500) or a mouse anti-TfR1 antibody (ThermoFisher #13-6800, 1:500) or a secondary anti-rabbit HRP (Promega #W4011 ...
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bioRxiv - Plant Biology 2021Quote: ... was carried out to determine the relative expression levels of 13 selected genes on StepOne™ Real-Time PCR System (Thermo Fisher Scientific) using Maxima SYBR Green ...
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bioRxiv - Microbiology 2022Quote: ... which were cut into small pieces of ∼5 × 5 mm and sterilised with 100% ethanol (for light microscopy and SEM) or 13 mm round Thermanox plastic coverslips (Nalgene Nunc International, Rochester, NY) scratched with sandpaper and sterilised with 100% ethanol (for SBFSEM ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was harvested from mouse islets and INS1(832/13) cells by using a QIAGEN RNeasy Plus Kit (Cat# 74034) and a TRIzol™ reagent (Invitrogen, cat#15596018) respectively ...
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bioRxiv - Neuroscience 2022Quote: ... were stained for hematoxylin and eosin or immunostained for glial fibrillary acidic protein (GFAP; rat-anti-GFAP; #13-0300 Thermo Fisher Scientific; RRID:AB_2532994), ionizing calcium binding adaptor protein 1 (IBA1 ...
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bioRxiv - Microbiology 2022Quote: ... cultures were diluted to an OD750 nm = 1.0 in 200 μL of the respective medium and monitored with multipoint absorbance measurements (13 coordinates, OD750 nm) over 20 min on a Varioskan Flash microplate reader (Thermo Fisher Scientific, Germany). Microplates were kept in the reader at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells treated with the indicated concentrations of selected compounds for 72 h in 100 μL of incubation media were supplemented with 20 μL of 0.6 mM resazurin (Acros Organics 62758-13-8) and incubated for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Biobanking and long-term storage of GBOs25,83 was performed by transferring small GBO pieces (0.1 – 0.2 mm3) to GBO culture medium containing 10% DMSO in cryogenic vials (Thermo Fisher Scientific, 13-700-504). Vials were stored in a foam Cell Freezing Container (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were blocked in 200 μL buffer W (GeoMx RNA Slide Prep FFPE-PCLN kit) for 30 minutes and incubated at 4°C overnight with SYTO 13 nucleic acid stain (500 nM; Thermo Fisher Scientific #S7575) and antibodies for pan-cytokeratin (panCK ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were again re-blocked with 5% NGS for 2 hr (second serum blocking) and subsequently stained with rat anti-GFAP (1:500; Invitrogen Cat. #13-0300), and rabbit anti-Megf10 (1 ...
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bioRxiv - Cell Biology 2024Quote: ... hiPSC colonies were detached in clusters using a 13 min incubation with collagenase type IV (250 IU/mL final concentration) (Thermo Fisher Scientific; 17104019) and transferred to 6-well low adhesion plates in ESC media supplemented with 10 µM ROCK Inhibitor (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... I/1Ki (boa constrictor kidney cells)81 and I/1Ki-Δ (refered to as I/1Ki/SDeV in this paper)13 were maintained in Minimal Essential Medium Eagle (MEM; Gibco #31095-029) supplemented with 10% heat-inactivated fetal calf serum (Sigma-Aldrich #F75424) ...
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bioRxiv - Bioengineering 2023Quote: ... Donor blood (20 mL) was collected in a sterile fashion into BD vacutainer spray coated K2EDTA tubes (Fisher Scientific, cat. 13-680-61) and PBMCs were isolated using density gradient centrifugation on Histopaque and expanded by using StemPro-34 Serum Free Complete Media (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... 25,000-50,000 cells were plated and transfection of specified constructs or empty vector (6-13 µg/nL) was performed with Lipofectamine 2000 (Thermo Fisher Scientific, 1166819) and Opti-MEM (Gipco ...
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bioRxiv - Biochemistry 2023Quote: ... 5 μL of each sample was pooled together to make a “pooled sample” for a given derivatization batch and 80 μL of each individual sample were loaded into a 150 μL glass insert (CAS#13-622-207, Thermo scientific, Florence, KY) inside of a GC vial with a 9 mm PTFE/red rubber septum (CAS#C4000-30 ...
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bioRxiv - Microbiology 2023Quote: Axenic haptomonad cells were generated by culturing 1 × 106 cells/ml promastigotes on 13 mm round Thermanox plastic coverslips (Nalgene Nunc International, Rochester, NY) scratched with sandpaper and sterilised with 100% ethanol (for proteomics and electron microscopy ...
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bioRxiv - Genetics 2023Quote: ... amount of diluted Cas9 Nuclease (Cat#1081058) and gRNA:tracrRNA duplex were mixed with 13 µL of Opti-MEM Medium (Thermo Fisher Scientific, Cat#51985091) and incubated for 5 minutes at room temperature ...
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bioRxiv - Pathology 2024Quote: ... Oligos were resuspended into a 10 µM working stock and set up in a PCR reaction to prime to the CRISPR tail (primer 13) with dNTPs and high-fidelity polymerase (Phusion polymerase, Thermo Scientific #F-530S) (Ta = 55 °C ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were then blocked with 1% bovine serum albumin in PBS for 1 hour before incubating with rat anti-GFAP (Thermo Fisher, 13-0300) or rabbit anti-cFos (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2024Quote: ... We prepared matched amino acid-replete and amino acid-free media in the following way: Amino acid-free DMEM (US Biologicals, D9800-13) containing 10% dialyzed FBS (Life Technologies, A33820-01) served as our amino acid deprivation media and as our base media for making complete media ...