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Citations for Thermo Fisher :
3501 - 3550 of 9363 citations for IL 13 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Detection was further enhanced with an anti-rabbit biotin antibody (Thermo Scientific, Rockford, IL) and AlexaFluor-647 streptavidin-conjugated antibody (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... and protein A/G agarose (#20422) were purchased from Thermo Scientific (Rockford, IL, USA). Recombinant human decorin protein (#143-DE-100 ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were treated with Pierce ECL Western Blotting Substrate (Thermo Scientific, Rockford, IL) and exposed to film.
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bioRxiv - Neuroscience 2020Quote: ... Total proteins were quantified by using BCA kit (Pierce, Thermo Scientific IL 61101 USA). ELISA for IGF-1 (DuoSet ELISA kit ...
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bioRxiv - Developmental Biology 2021Quote: ... 1.25 µL of DMSO (D128-500, Fisher Chemical, Fisher Scientific, Hanover Park, IL, USA), 0.125 µL of Taq (42-800B3 ...
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bioRxiv - Developmental Biology 2021Quote: ... Homemade platinum electrodes (78-0085, Strem Chemicals Inc., Fisher Scientific, Hanover Park, IL, USA) mounted in an Adjustatrode holder (01-925-09 ...
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bioRxiv - Microbiology 2021Quote: ... total RNA was measured using a Nanodrop 1000 Spectrophotometer (Thermo Scientific, Rockford, IL, USA) and was reverse transcribed into cDNAs using oligo-d(T ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentration was determined by BCA protein assay kit (Thermo Scientific, Rockford, IL., USA). Equivalent amounts of protein were resolved by 10% SDS-PAGE and transferred to polyvinylidene difluoride membrane ...
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bioRxiv - Immunology 2020Quote: ... in the presence of 5 or 50 ng/ml IL-21 (Thermo Fisher Scientific) as indicated ...
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bioRxiv - Immunology 2020Quote: IL-8 secretion was measured using an ELISA kit (Life Technologies; 88-8086-88). Cell-free supernatant was harvested and stored at −20°C until assayed by ELISA ...
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bioRxiv - Immunology 2020Quote: ... Plate was then washed again and incubated with IL-2 monoclonal antibody (ThermoFisher, USA) for 2 hours at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... Sample protein concentrations were measured (Pierce BCA Protein Assay Kit, Thermo Scientific, Rockford, IL), and volumes were adjusted to normalize microgram per microliter protein content ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysate was prepared with Pierce™ RIPA buffer (89900, Thermo Scientific, Rockford, IL) containing Protease and Phosphatase Inhibitor Cocktail (1861281 ...
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bioRxiv - Pathology 2022Quote: Total protein was quantified using the BCA protein assay kit (Thermo Scientific, Rockford, IL). Western blotting was done as previously described 16 ...
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bioRxiv - Immunology 2022Quote: ... and IL-1β concentrations were measured using a ProcartaPlex simplex assay (ThermoFisher, Waltham, MA).
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bioRxiv - Pathology 2022Quote: ... Primary antibodies were replaced by normal rabbit IgG or mouse IgG (Invitrogen, Rockford IL) and included in each staining series as the negative control ...
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bioRxiv - Neuroscience 2022Quote: ... and gene expression was analyzed using TaqMan probes (ThermoFisher Scientific Inc., Rockford, IL USA) using a ViiATM7 Real Time PCR System or QuantStudio 6 FLEX Real Time PCR System (ThermoFisher Scientific Inc. ...
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bioRxiv - Neuroscience 2022Quote: ... that contains a Halt Protease Inhibitor Cocktail (catalog#1861281, Thermo Scientific, Rockford, IL, USA). Cultured neurons cells were lysed at day 8 of differentiation ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 500 U/ml IL-2 and 1X Glucose Solution (Thermo Fisher Scientific). Cell counts were analyzed every six hours using the Incucyte Live Cell Analysis System (Essen BioScience) ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were placed on gelatin-coated glass slides (SuperFrost Plus, Thermo Fisher Scientific, IL) and submerged in 0.5% cresyl violet solution (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... we added 0.95 µg of MS grade trypsin (Thermo Scientific Pierce, Rockford, IL, USA) mixed in 40 µL of ABC to each filter and incubated at 37°C for 16 hours ...
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bioRxiv - Microbiology 2024Quote: ... The membranes were treated with Pierce ECL western blotting substrate (Thermo Scientific, Rockford, IL) and exposed to film.
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bioRxiv - Molecular Biology 2024Quote: ... we added 0.95 µg of MS grade trypsin (Thermo Scientific Pierce, Rockford, IL, USA) in 40□µl of 50 mM ammonium bicarbonate to the filters and incubated 13.5 hours in a wet chamber at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... The plasmids were then extracted with GeneJET Plasmid Miniprep Kit (Thermo Scientific, Rockford, IL). The PCR products and plasmid insertions were confirmed without unexpected mutations by using Sanger sequencing.
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bioRxiv - Bioengineering 2023Quote: ... the cells were challenged with 1 ng/mL murine IL-1α (400ML005CF; Fisher Scientific). 500µL of culture media was collected and snap frozen at 0 ...
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bioRxiv - Molecular Biology 2023Quote: ... cell lysis was performed by adding M-PER lysis buffer (Thermo Scientific; Rockford, IL) supplemented with 1x cOmplete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... Electroporation was performed using the NEON Transfection System (Thermo Fisher Scientific, Rockford, IL, USA), according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... The proteins and peptides were visualized using chemiluminescent substrates (Thermo Scientific, Rockford, IL, USA) using a ChemiDoc MP imaging system (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... HALT Protease and Phosphatase Inhibitor Cocktail (1:100, 1861280, Thermo Scientific, Rockford, IL, USA), pepstatin A (1:100 ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed with RIPA lysis and extraction buffer (ThermoFisher Scientific, Rockford, IL, USA), supplemented with 1x protease inhibitor (Pierce Protease Inhibitor Tablet ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibodies: cardiac troponin T mouse monoclonal antibodies (cTnT: Thermo Scientific, Rockford, IL, USA), diluted to 1/200 with 3% BSA ...
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bioRxiv - Cancer Biology 2023Quote: ... ELISAs were performed to measure the release of IL-2 (Invitrogen, #88-7025-88), IFN-γ (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Cells were then incubated with IL-17A FITC (clone: eBio17B7, 11-7177-81; Invitrogen), IL-13 PE (clone ...
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bioRxiv - Cancer Biology 2024Quote: ... ECL solution (entry-level peroxidase substrate for enhance chemiluminescence) (Thermo Scientific, Rockford, IL, USA) was loaded on the membrane in order to allow the chemiluminescent reaction between horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2024Quote: ... Pierce bicinchoninic acid (BCA) protein assay kit was purchased from Thermo Scientific (Rockford, IL). Ethyl alcohol (190 proof ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then fixed with 500μL 4% paraformaldehyde (PFA) (Thermo scientific, Rockford, IL, USA) solution in PBS 1X ...
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bioRxiv - Immunology 2024Quote: ... Conventional PCR was performed using DreamTaq Green DNA Polymerase (Thermo Scientific, Rockford, IL, USA), following manufacturer’s indications ...
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bioRxiv - Bioengineering 2024Quote: ... Concentration of IL-12 p70 in serum was determined using mouse ELISA Kit (Invitrogen) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: ... Pierce bicinchoninic acid (BCA) protein assay kit was purchased from Thermo Scientific (Rockford, IL). NuPAGE Bis-Tris precast gels were purchased from Life Technologies (Carlsbad ...
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bioRxiv - Biophysics 2020Quote: MDA-MB-231 breast cancer cells were cultured in modified Eagle’s medium (MEM-F10, c.c.pro) supplemented with 10% fetal calf serum (FCS, Invitrogen) and 2.5 mM L-glutamin (c.c.pro ...
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bioRxiv - Biophysics 2020Quote: ... cell medium was replaced by 1 ml Leibovitz’s L-15 Medium (c.c.pro) containing 10% FCS and 25 nM Hoechst 33342 nucleic acid stain (Invitrogen) and incubated for 1 h at 37 °C before imaging.
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bioRxiv - Cancer Biology 2021Quote: ... After washing three times with MACS buffer consisting of PBS supplemented with 5 % FCS (Thermo Fisher Scientic) and 2 mM EDTA to remove unbounded cells ...
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bioRxiv - Immunology 2021Quote: ... and 10% fetal calf serum (FCS, Biowest) and minced into pieces of 1 mm3 in PBS (Gibco). The biopsies were enzymatically digested with 1 mg/mL collagenase IV (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... USA) supplemented with 10% fetal calf serum (FCS) and 1% penicillin-streptomycin solution (Invitrogen, Carlsbad, CA, USA) and were incubated at 37□ in a humidified atmosphere containing 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were pre-incubated with anti-Fc receptor antibody (Thermal Fisher Scientific, Cat. No. 14-9161-73) at 1:20 ratio to block Fc receptor before staining ...
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bioRxiv - Immunology 2021Quote: ... and the absorbance (OD492nm) was measured using a Multiskan-FC microplate reader (Thermo Fisher Scientific, Helsinki, Finland). Ligation of the recombinant proteins was compared to the SUMO control.
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bioRxiv - Immunology 2019Quote: ... resuspended in William’s E medium containing 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific) and plated ...
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bioRxiv - Microbiology 2019Quote: ... in Dulbecco’s Modified Eagle medium (DME) supplemented with 1% v/v fetal calf serum (FCS) (Invitrogen, Australia) and 1% v/v Glutamax (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 % non-essential amino acids and sodium pyruvate and 30 % fetal calf serum (FCS) (Gibco, 10270-106). When cells reached confluency ...
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bioRxiv - Neuroscience 2020Quote: ... The absorbance at 450 nm wavelength was read on a microplate reader (Multiskan FC, Thermo Fisher Scientific). Mean values were calculated for triplicate sets of standards and samples ...