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Citations for Thermo Fisher :
3551 - 3600 of 5196 citations for Tryptase gamma Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The peptide ions were detected under the data-dependent acquisition mode using the installed Xcalibur software (Thermo Fisher Scientific). Full-scan mass spectra were acquired in the range of 375-1,500 m/z with resolution of 70,000 ...
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bioRxiv - Genomics 2022Quote: ... Peptide fractions were analyzed using the Orbitrap Fusion MS coupled to a Dionex Ultimate 3000 nHPLC system (Thermo Fisher Scientific Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... the LePin peptides are coupled to an affinity column with SulfoLink® Immobilization Kit (Thermo Scientific™, Cat. 44995) according to the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were online desalted on a trapping cartridge (Acclaim PepMap300 C18, 5 μm, 300Å wide pore, Thermo Fisher Scientific) for three minutes using 30 uL/min flow of 0.05% TFA in water ...
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bioRxiv - Molecular Biology 2023Quote: ... Identifications and retention times were used to guide the manual quantification of each modified peptide using QualBrowser version 2.0.7 (Thermo Scientific). Site assignment was evaluated from MS2 spectra using QualBrowser and MaxQuant Viewer ...
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bioRxiv - Microbiology 2023Quote: ... peptide quantitative features were extracted from LC-MS files using SIEVE (v2.2, Thermo Fisher Scientific, San Jose, CA, USA) and processed by the UHR-IonStar APP to generate final quantification results ...
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bioRxiv - Molecular Biology 2022Quote: The peptide samples were analysed on a nano liquid chromatography system (Ultimate 3000 nano HPLC system, Thermo Fisher Scientific) coupled to an Orbitrap Exploris 240 mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptide separation was performed using a reversed-phase analytical column (Acclaim PepMap RSLC, 0.075 x 250 mm, Thermo Scientific) with a linear gradient of 4%-27.5% solvent B (0.1% FA in 98% ACN ...
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bioRxiv - Molecular Biology 2023Quote: ... The desalted peptides were labeled with the TMT11plex mass tag labeling reagent according to the manufacturer’s instructions (ThermoFisher Scientific) with small modifications ...
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bioRxiv - Biochemistry 2022Quote: The peptide samples were analyzed on a nano liquid chromatography system (Ultimate 3000 nano HPLC system, Thermo Fisher Scientific) coupled to an Orbitrap Exploris 240 mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: Peptides from 833 µm resolution samples were analysed by LC-MS/MS using a Dionex Ultimate 3000 (Thermo Scientific) coupled to a timsTOF Pro (Bruker ...
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bioRxiv - Microbiology 2023Quote: ... Peptide samples were loaded onto a 75 µm x 2 cm Acclaim PepMap 100 C18 trap column (Thermo Scientific) in 0.1% formic acid ...
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bioRxiv - Systems Biology 2023Quote: ... Final peptide concentration was estimated by measuring absorbance at 280 nm on a NanoDrop 2000C spectrophotometer (Thermo Fisher Scientific). The resulting peptide mixtures from yeast were desalted by Stage-tips ...
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bioRxiv - Biochemistry 2023Quote: ... The searched results were filtered with a 1% false discovery rate and the detected peptides were confirmed by manual analysis for monoisotopic mass accuracy and retention time with Xcalibur 2.2 (ThermoFisher). Fragment ions were also manually confirmed and were accepted based on the presence of a monoisotopic mass within 20 ppm mass accuracy ...
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bioRxiv - Cell Biology 2023Quote: ... approximately 1 μg of peptides were loaded on a C18 precolumn (PepMap100, 5 μm, 300 Å, Thermo Fisher Scientific) and separated over a C18 column (50 cm × 75 μm ...
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bioRxiv - Cell Biology 2023Quote: Samples were pooled in equimolar ratio and subjected to High pH Reversed-Phase Peptide Fractionation kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Dissolved peptides were added to Fe-NTA Magnetic Agarose (80 µL of suspension used; Cat # A52284, Thermo Fisher Scientific) washed with 80% ACN in 0.1% TFA and mixed for 60 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: Fe-NTA enrichment was carried out with a High Select Fe-NTA phospho-peptide enrichment kit (Thermo Fisher Scientific), as previously described29,30 ...
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bioRxiv - Molecular Biology 2023Quote: ... The trapped peptides were separated on an Acclaim PepMap RSLC nanoViper column (75µm x 500mm, C-18, 2µm, 100Å, Thermo Fisher) at 300 nL/min flow rate and 40 °C column temperature using water and acetonitrile with 0.1% formic acid as solvents A and B ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were separated using a 140 min gradient generated by an EASY-nLC 1200 liquid chromatography (Thermo Fisher Scientific) heated to 50 °C by a PRSO-V1 column oven (Sonation) ...
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bioRxiv - Cancer Biology 2023Quote: ... peptides were directly sprayed into an Orbitrap Fusion Lumos mass spectrometer equipped with a nanoFlex ion source (ThermoFisher Scientific).
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bioRxiv - Microbiology 2023Quote: ... Peptides were separated using a 140 min gradient generated by an EASY-nLC 1200 liquid chromatography (Thermo Fisher Scientific) heated to 50 °C by a PRSO-V1 column oven (Sonation) ...
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Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: ... The multiplexed peptide sample was subjected to high pH-reversed-phase fractionation according to the manufacturer’s instructions (Thermo Fisher). In this protocol ...
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bioRxiv - Cell Biology 2023Quote: ... HDFs were cultured for 72 hours in the presence of each peptide (at 3-12.5 ppm)in Dulbecco’s modified Eagle’s medium (DMEM, Gibco #21969-035) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2023Quote: ... peptides were first loaded onto a trap cartridge (ThermoFisher PepMap, C18, 5 μm, 100 μm i.d. x 20 mm), then eluted onto a reversed phase Easy-Spray column (ThermoFisher PepMap ...
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bioRxiv - Systems Biology 2023Quote: ... with 2 μm bead size was used for peptide separation in an EASY-nLC 1200 HPLC (Thermo Fisher Scientific). Peptides were separated in a gradient composed as follows ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted peptides were sprayed directly by electrospray ionization into a Q Exactive Plus Orbitrap mass spectrometer (Thermo Scientific). Mass spectrometry measurement was conducted in data-dependent acquisition mode using a top10 method with one full scan (mass range ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were then separated (EASY-Spray; PepMap RSLC C18, 75 μm × 50 cm, 2 μm particles, Thermo Fisher Scientific) at a flow rate of 250 ηL min-1 employing a two-step gradient ...
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bioRxiv - Neuroscience 2023Quote: ... was used to elute peptides from the capillary reverse-phase column (0.075 × 150 mm, Pepmap®, Thermo Fisher Scientific). Data were acquired using the Xcalibur software ...
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bioRxiv - Biochemistry 2023Quote: We labeled 10 µg of tryptic peptides from each experiment with a TMT10plex Isobaric Labeling Reagent kit (Thermo Scientific) (Zecha et al. ...
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bioRxiv - Systems Biology 2023Quote: ... the dried peptides were dissolved in 250 µL of buffer A (0.1% formic acid in H2O) (Thermo Fisher Scientific) with 1:2500 (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... Chromatographic separation of the peptides was performed by liquid chromatography on a nano-HPLC system (Ultimate 3000, Thermo Scientific) using a nano-LC column (Acclaim PepMapC100 C18 ...
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bioRxiv - Cell Biology 2023Quote: ... An aliquot containing ∼ 100 µg of peptides was removed and labeled with Tandem-Mass-Tag (TMT) reagent (ThermoFisher Scientific). Once labeling efficiency was confirmed to be at least 95% ...
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bioRxiv - Microbiology 2023Quote: ... and tryptic peptides were cleaned using a C18 column and dried with a SpeedVac concentrator (Thermo Fisher Scientific, USA). The peptides were quantified using the Pierce Quantitative Colorimetric Peptide Assay Kit ...
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bioRxiv - Microbiology 2023Quote: ... USA).The resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (ThermoFisher Scientific, Rockford, IL, USA) at the Vienna Research Platform for Metabolomics & Proteomics and analyzed using the Proteome Discoverer v2.2.0.388 (ThermoFisher Scientific ...
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Short-range interactions between fibrocytes and CD8+ T cells in COPD bronchial inflammatory responsebioRxiv - Pathology 2023Quote: ... Each peptide extracts were loaded on a 300 µm ID x 5 mm PepMap C18 precolumn (Thermo Scientific, USA) at a flow rate of 10 µL/min ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were separated using a 140 min gradient generated by an EASY-nLC 1000 liquid chromatography (Thermo Fisher Scientific) with the column heated to 50 °C by a PRSO-V1 column oven (Sonation) ...
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bioRxiv - Microbiology 2023Quote: ... Peptide separation was carried out using an Acclaim PepMap RSLC C18 column (75 μm × 50 cm; Thermo Fisher Scientific) using standard reverse-phase gradients ...
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bioRxiv - Microbiology 2023Quote: The peptide mixture from the biological replicates was analyzed by LTQ Velos Orbitrap mass spectrometer (Thermo Fisher Scientific, USA) coupled with liquid chromatography-tandem mass spectrometry using an EASYnLC system (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Peptides were gradient eluted from the column directly to Eclipse mass spectrometer using a 1 hour gradient (Thermo Scientific) using 2% acetonitrile and 0.5% acetic acid for solvent A and 80% acetonitrile and 0.5% acetic acid for solvent B.
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bioRxiv - Molecular Biology 2023Quote: ... applying settings from the literature for analyzing HLA peptides (Bassani-Sternberg et al. 2015) and ProteomeDiscoverer 2.3 software (Thermofisher). Raw data has been deposited to ProteomeXchange Consortium via the MassIVE partner repository with data set identifier PXD042439.
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bioRxiv - Biochemistry 2023Quote: Tryptic peptides were analyzed by LC-MSMS using a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen, Germany). Peptide mixtures were fractionated by an Ultimate 3000 RSLCnano (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... The peptides were first concentrated on a precolumn (PepMap100 C18 3 μm; 75 μm × 2 cm; Thermo Fisher Scientific) and then separated on an EASY-Spray column (ES903 ...
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bioRxiv - Bioengineering 2023Quote: The peptide digests were subjected to LC MS/MS analysis using an UltiMate 3000 RSLC system (Thermo Fisher Scientific) coupled in-line to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Tryptic peptides were loaded onto a trap column (Pepmap 100 5μm, 5 × 0.3 mm, ThermoFisher Scientific, San Jose, CA) at a flow rate of 10 μL/min using 0.1% TFA as loading buffer ...
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bioRxiv - Immunology 2023Quote: ... Separation of peptides was achieved using a DNV PepMap Neo separation column (75 µm x 150 mm, Thermo Scientific) and a gradient from 1 % to 40 % B within 120 min and a flowrate of 400 nL/min at 60 °C ...
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bioRxiv - Immunology 2023Quote: ... LC/MS data acquisition parameters: Peptides were separated and analyzed on an EASY nLC 1200 System (Thermo Fisher Scientific) in-line with the Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... LC/MS data acquisition parameters: Peptides were separated and analyzed on an EASY nLC 1200 System (Thermo Fisher Scientific) in-line with the Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: The fractionated peptides were analysed by LC-MS/MS using a fully automated Ultimate 3000 RSLC nano System (ThermoFisher) fitted with a 100 μm x 2 cm PepMap100 C18 nano trap column and a 75 μm×25 cm ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were then vacuum dried and concentration was determined by measuring the absorbance at 280/260 (NanoDrop, Thermo Scientific). 200μg of peptides in 100mM HEPES pH=8-5 were then labeled with 400µg of 11-plex TMT (Thermo ...