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Citations for Thermo Fisher :
3801 - 3850 of 5196 citations for Tryptase gamma Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were injected and eluted through a 50 cm EASY-Spray column PepMap RSLC C18 (Cat. ES803; Thermo Fisher Scientific) with a 135-min gradient at constant 300 nL/min kept at 40°C ...
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bioRxiv - Immunology 2022Quote: ... Reconstituted peptides were tested for endotoxin contamination using a Limulus Amebocyte Lysate Chromogenic Endotoxin Quantitation Kit (Thermo Scientific, UK #88282).
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bioRxiv - Immunology 2022Quote: ... next peptides were fractionated based on their molecular mass using ultra performance liquid chromatography (UltiMate-3000 system, Thermo Fisher Scientific) and finally desalted and acidified on a C-18 cartridge (3M ...
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bioRxiv - Genetics 2019Quote: ... LC-MS/MS data acquisition: Peptide samples were analyzed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific) equipped with a nano-electrospray ion source and a nano-flow LC system (Easy-nLC 1000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The combined filtrates were desalted on a high pH reversed-phase peptide fractionation column (Pierce™, Thermo Fisher Scientific, USA). Then the peptides were collected followed by vacuum drying ...
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bioRxiv - Neuroscience 2019Quote: ... The peptides were separated and analyzed on a Dionex Ultimate 3000 RSLCnano system coupled to a Q-Exactive (Thermo Fisher). Approximately 2μg peptide from each fraction was injected into an Acclaim peptide trap column (Thermo Fisher ...
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bioRxiv - Microbiology 2019Quote: ... Tryptic peptides were measured by nLC-MS/MS (Ultimate 3000 coupled to a Q Exactive, Thermo Fisher Scientific, Waltham, USA) applying a 3 h method (∼140 min elution window) ...
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bioRxiv - Molecular Biology 2019Quote: ... The peptides were concentrated (on-line) by reverse phase chromatography using a 0.1 mm × 20 mm C18 RP precolumn (Thermo Scientific), and then separated using a 0.075mm x 250 mm C18 RP column (Thermo Scientific ...
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bioRxiv - Biophysics 2019Quote: DSP crosslinked peptides liberated by EDT fragmentation were identified by Sequest searches of MS3 scans using Protein Discoverer 1.4 (Thermo Scientific). The searches were performed using a Swiss Prot rat database downloaded in May 2015 containing 8078 entries supplemented with the sequences of hamster SUR1 ...
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bioRxiv - Biochemistry 2019Quote: ... The sample was dissolved in 300 µl of 0.1% TFA solution in water and subjected to fractionation using High pH Reverse-Phase Peptide Fractionation Kit (Thermo scientific) to 8 fractions and dried on a speedvac.
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bioRxiv - Cell Biology 2019Quote: ... Approximately 150 μg of peptides for each sample was labeled with 200 μg of TMT-6plex reagent (Thermo Fisher Scientific) for 1 h at room temperature ...
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bioRxiv - Systems Biology 2019Quote: ... Liquid Chromatography (LC) separation of the peptides was performed on an Ultimate 3000 Dionex RSLC-nano UHPLC (Thermo Fisher Scientific), equilibrated with 95% solvent A and 5% solvent B (equilibration solution) ...
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bioRxiv - Immunology 2020Quote: ... The peptide samples derived from individual patients were then individually labelled using the TMT10plex mass tag kit (Thermo scientific, USA) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The antibody was purified by affinity chromatography using the peptide immobilized on an agarose resin via the Sulfo-Link chemistry according to the manufacturer’s instruction (Thermo Scientific). The antibodies were eluted sequentially with a 0.2 M glycine solution at pH 3 and pH 2.5 ...
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bioRxiv - Cancer Biology 2020Quote: A synthetic peptide labeled with heavy arginine (13C, 15N) was formulated for the MDSTEPPYSQK(me3)R (eEF1A1_K318) peptide (HeavyPeptide AQUA custom synthesis service, Life Technologies). Synthetic peptides were spiked into the WT and SETD2-KO lysates from the HKC and 786-O cells ...
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bioRxiv - Plant Biology 2020Quote: High pH reverse phase fractionation was performed using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Scientific PN84868) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... with a N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The recombinant baculoviruses were generated according to the manufacture’s instruction to infect Hi5 cells at a density of 2×106 cells/ml ...
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bioRxiv - Biochemistry 2021Quote: ... Separated peptides were directly eluted into a nanospray ion source of an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific). The stainless steel emitter spray voltage was set to 2200 V ...
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bioRxiv - Biochemistry 2020Quote: ... An integrated loading pump was used to load peptides onto a trap column (Acclaim PepMap 100 C18, 5 um particles, 20 mm length, ThermoFisher) at 5 µL/min ...
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bioRxiv - Cell Biology 2021Quote: ... Data-dependent LC-MS/MS analysis of extracted peptide mixtures after digestion was carried out with a LTQ-Velos PRO orbitrap mass spectrometer (ThermoFisher) interfaced to a nanocapillary HPLC (RSLC3000 ...
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bioRxiv - Cell Biology 2021Quote: The triptic peptides were analyzed by a LTQ XL-Orbitrap ETD equipped with a HPLC NanoEasy-PROXEON (Thermo Fisher Scientific). They were loaded ...
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bioRxiv - Biochemistry 2021Quote: ... The desalted tryptic peptide samples were loaded onto an Acclaim PepMap 100 pre-column (75 μm x 2cm, ThermoFisher Scientific) and separated by Easy-Spray PepMap RSLC C18 column with an emitter (2 μm particle size ...
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bioRxiv - Neuroscience 2021Quote: ... and the supernatant containing the peptides was collected and dried down in a centrifugal evaporator (Savant SPD 1010, Thermo scientific). The peptide mixtures were reconstituted in a solution of 2% (v/v ...
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bioRxiv - Physiology 2020Quote: ... An aliquot (10%) was removed for quantification using the Pierce Quantitative Fluorometric Peptide Assay kit (Thermo Scientific, Cat. No. 23290). The remainder of the peptides from each IP samples (∼0.5-3.5 µg ...
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bioRxiv - Plant Biology 2021Quote: Desalted peptides were analyzed by liquid chromatography coupled mass spectrometry using a Q Exactive Plus mass spectrometer (Thermo Fisher Scientific), coupled online to a RSLC nano HPLC (Ultimate 3000 ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were trapped and desalted on a C18-column (5 μm Acclaim PepMap100 300 μm x 5 mm, ThermoFisher Scientific) at a flow rate of 30 μl/min with solution A (1% acetonitrile (ACN) ...
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bioRxiv - Cell Biology 2021Quote: ... Desalted peptides were dissolved in 0.1% (v/v) formic acid and injected onto an Easy-nLC 1000 (Thermo Fisher Scientific), coupled to an Orbitrap Exploris 480 (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... The peptides were then analyzed by LC-MS/MS using a LTQ-Orbitrap Fusion Tribride mass spectrometer (Thermo Fisher Scientific). Peptide identification was performed using Proteome Discoverer v1.4.1.14 (Thermo Scientific ...
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bioRxiv - Plant Biology 2020Quote: ... Specific peptide sequences unique for the target proteins (AtpA, AtpB, AtpE) were created using the Pinpoint software (version 1.0, Thermo Scientific) and are listed in Supplemental Table 3 ...
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bioRxiv - Microbiology 2020Quote: ... by EASYnLC and the peptides separated through an Acclaim PepMap RSLC column (0.075 mm x 150 mm C18, Thermo Scientific) with the same gradient as above ...
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bioRxiv - Systems Biology 2020Quote: ... Desalted peptides were reconstituted in 50 mM HEPES (pH 8.5) and labelled with TMT10plex158 Isobaric Label Reagent (Thermo Fisher Scientific) according the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were eluted with a 60% acetonitrile (ACN)/0.1% TFA solution and dried in a SpeedVac vacuum concentrator (Thermo Fisher). For the IP of di-glycine peptides ...
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bioRxiv - Neuroscience 2020Quote: ... A spectral library was created in Skyline for some of the target peptides using several DDA of FMRpolyG samples as well as blanks spiked with AQUA Ultimate endogenous (light) and isotopically labeled (heavy) peptides obtained from ThermoFisher Scientific (Supplementary Table 1) ...
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bioRxiv - Microbiology 2020Quote: ... An integrated loading pump was used to load peptides onto a trap column (Acclaim PepMap 100 C18, 5 um particles, 20 mm length, ThermoFisher) at 5 µL/min ...
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bioRxiv - Microbiology 2021Quote: ... Peptides were infused directly from the column tip into the on-line coupled Q Exactive HF mass spectrometer (Thermo Scientific) using nanoelectrospray ion source (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The chromatographic separation of the peptides was achieved using a 50 cm long C18 Easy spray column (Thermo Scientific,US) at 55°C ...
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bioRxiv - Microbiology 2020Quote: ... 200 μL/min flow rate) was used for peptide separation right followed by LTQ-FT mass spectrometry analysis (ThermoFisher Scientific) with data acquisition at a mass resolving power of 100,000 at m/z 400 ...
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bioRxiv - Microbiology 2020Quote: ... peptides were recovered in HEPES buffer by collecting supernatant on magnet and combining it with a second elution wash of the magnetic beads with HEPES buffer. Peptides were labelled with TMT10plex (Werner et al. 2014) Isobaric Label Reagent (ThermoFisher) according the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: The digested peptides were analyzed by reverse phase LC-MS/MS on an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) coupled to a Dionex UltiMate 3000 -nLC (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were then introduced by nanoelectrospray into a LTQ Orbitrap Velos Pro coupled to an Easy-nLC HPLC (Thermo Fisher) over a linear gradient of acetonitrile at 250 nl/min over 140 min ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides from each sample were labelled using the ten-plex TMT reagent kit (90110, Thermo Fisher Scientific, Waltham, MA, US). Two ten-plex experiments were performed ...
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bioRxiv - Biophysics 2020Quote: ... or RGD-containing peptide (Peptite-2000; 100 μg mL−1 in PBS) in 1× PBS with Ca2+ (DPBS, Catalog No. 14040133, Gibco). Unattached beads were removed by washing with 1× PBS.
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bioRxiv - Biophysics 2021Quote: ... were vacuum-dried and resuspended with the peptide fractionation buffer [(70% (v/v) LC-MS grade water (Thermo Fisher Scientific), 30% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... The first set of LC-MS/MS was used to identify highly abundant unoxidized peptides using the Sequest search engine (Proteome Discoverer 1.4, Thermo Scientific) against the Uniprot Rattus Norvegicus database (downloaded on March ...
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bioRxiv - Biochemistry 2022Quote: ... and the resulting peptides were trapped and desalted on a 2 mmL×L10 mm C8 trap column (Hypersil Gold, ThermoFisher). The bound peptides were then gradient-eluted (4-40% CH3CN v/v and 0.3% v/v formic acid ...
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bioRxiv - Cancer Biology 2022Quote: Peptides were injected onto a trapping column for preconcentration (Acclaim Pepmap100 20cm x 75 μm, 3μm C18, Thermo Fisher Scientific), followed by nanoflow LC (Thermo Dionex ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Identifications and retention times were used to guide the manual quantification of each modified peptide using QualBrowser version 2.0.7 (Thermo Fisher Scientific). Site assignment was evaluated from MS2 spectra using QualBrowser and MaxQuant Viewer ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted peptides were then subjected to electrospray ionization directly coupled to a high-resolution Orbitrap mass spectrometer (QExactive, ThermoFisher).
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bioRxiv - Immunology 2022Quote: ... were pulsed for 1 hr with 10μg/ml influenza virus NP(366-374) peptide and were subsequently labeled for 10 min at 37°C with 5 μM CFSE (Invitrogen) (CFSEhigh ...
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bioRxiv - Neuroscience 2022Quote: ... Constant flow of 10 μl/min was maintained for peptide loading onto a pre-column (PepMap 100; Thermo Fisher Scientific). Peptides were then separated on a 10 mm PicoTip™ fused silica emitter 75 μm x 16 cm (New Objective Inc. ...