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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Total protein in brain tissue was determined using a protein assay kit (Pierce™ BCA Protein Assay Kit; ThermoFisher, 23225) following the manufacturer’s protocol.
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bioRxiv - Microbiology 2022Quote: ... Protein extraction was performed as described previously37 and protein concentration was determined using Pierce BCA protein assay kit (Thermo scientific).
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were measured using the BCA Protein Assay Kit (Pierce™ BCA Protein Assay kit, Thermo Fisher Scientific, 23225) and a Tecan Infinite M200 Plate Reader ...
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bioRxiv - Neuroscience 2024Quote: ... the supernatant containing the protein extract was collected and the total protein concentration was determined (BCA Protein Assay Kit, Thermofisher). Samples were reduced with DTT (15 mM ...
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bioRxiv - Biochemistry 2023Quote: ... Dried proteins were reconstituted in 0.2 % SLS and the amount of proteins was determined by bicinchoninic acid protein assay (Thermo Scientific). For tryptic digestion 50 μg protein was incubated in 0.5% SLS and 1 μg of trypsin (Serva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 °C) and the total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific, 23225) following manufacturer’s protocol ...
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bioRxiv - Physiology 2023Quote: ... Protein purity and concentration were determined using Coomassie Imperial Protein Stain and Pierce bicinchoninic acid protein assay (Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... Total protein quantification was determined using Pierce™ BCA Protein Assay Kit (Thermo Scientific Pierce™ BCA Protein Assay Kit) and samples were stored at -80°C until use.
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bioRxiv - Pathology 2023Quote: ... aortic protein lysates were prepared in RIPA lysis buffer and protein concentration was measured using the BCA Gold protein assay (ThermoFisher). Equal protein amounts (25μg ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein lysates were sonicated and protein concentration was determined using Pierce™ BCA Protein Assay Kit (Thermo Fisher, cat.no. 23225) for subsequent analyses.
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bioRxiv - Microbiology 2024Quote: ... Dried proteins were reconstituted in 0.2% SLS and the amount of proteins was determined by bicinchoninic acid protein assay (Thermo Scientific). For tryptic digestion 50 µg protein was incubated in 0.5% SLS and 1 µg of trypsin (Serva ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were washed 2 times with TBST and 1 time with TBS before being developed using SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific). Membranes were imaged using LAS ImageQuant System (GE Healthcare).
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bioRxiv - Molecular Biology 2020Quote: ... each membrane is soaked in SuperSignal® West Fempto/Pico maximum sensitivity substrate (equal parts Luminol and stable peroxide buffer, Thermo Scientific). Membranes were imaged using a GeneGenome XRQ Chemilluminescence imager (high quantium efficiency (QE ...
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bioRxiv - Cell Biology 2020Quote: ... secondary antibodies were added for 30 min at room temperature and the membranes were washed 3x 5 min in TBST again and developed with ECL (SuperSignal™ West Pico PLUS Chemiluminescent Substrate, Thermo Scientific, cat#34580 or SuperSignal™ West Femto Maximum Sensitivity Substrate ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were analyzed by SDS PAGE and Western blot using HRP-labeled secondary antibodies and SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific). Films with representative exposure times were scanned ...
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bioRxiv - Cell Biology 2020Quote: ... then incubated with appropriate primary antibodies and HRP or near-IR-conjugated secondary antibodies and visualized using chemiluminescence (SuperSignal West Femto Chemiluminescent Substrate; Thermo Fisher Scientific), or a Li-Cor Odyssey fluorescence scanner ...
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bioRxiv - Neuroscience 2021Quote: ... After washing again for 3x 5 min in PBS bands were visualized with luminol-based enhanced chemiluminescence HRP substrate (Super signal west dura, Thermo Fisher Scientific) and the ImageQuant LAS 4000 system (GE healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... The chemiluminescent Western blot detection kit (SuperSignal West Femto Chemiluminescent Substrate) and ATP determination kit were from Thermo Scientific (Rockford, IL, USA). The ELISA Kit for rat/mouse insulin ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with HRP-conjugated secondary antibody in 4% (w/v) skimmed milk in TTBS and visualized using SuperSignal West Pico PLUS (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... The membranes were then washed with TBS-T buffer followed by exposure to the appropriate horseradish peroxidase-conjugated secondary antibody for 1h and visualized on either Kodak X-ray film (GeneMate, F-9023-8×10) or BioRad ChemiDoc Imaging System using either SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, 34080) or ECL Prime Western Blotting Detection Reagent (Amersham ...
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bioRxiv - Microbiology 2019Quote: ... Grids overlaid with samples containing the inner membrane rings without the export apparatus were imaged at the Yale West Campus on a Titan Krios microscope (Thermo Fisher Scientific) operated at 300-kV ...
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bioRxiv - Biophysics 2019Quote: ... the membrane was rinsed 3×10 min in TBS-Tw and developed using SuperSignal West Femto Max Sensitivity Substrate (Thermo Fisher Scientific). Images were collected using a C-DiGit scanner (Licor Biosciences ...
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bioRxiv - Genetics 2019Quote: ... Horseradish peroxidase (HRP) conjugated secondary IgG antibodies (1:10000) were used together with the SuperSignal West Dura Extended Duration Substrate (Thermo Scientific #34076) to detect the protein bands.
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bioRxiv - Systems Biology 2019Quote: ... were detected using the appropriate secondary HRP-conjugated antibodies and visualized by enhanced chemiluminescence detection (Supersignal West Pico; Pierce - Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... the blots were incubated with the appropriate secondary horseradish peroxidase-conjugated antibody at room temperature for 1 h followed by detection using the SuperSignal West Pico PLUS reagents (Thermo Fisher Scientific). For a full list of antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... in TBS-T signal detection was performed (after incubating blots for 5 min with either Pierce ECL Pico or Super Signal West Femto substrate (Thermo Fisher Scientific)) with a ChemiDoc imaging station (BioRad) ...
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bioRxiv - Microbiology 2021Quote: ... Staining was revealed with corresponding horseradish peroxidase (HRP)-coupled secondary antibodies and developed using Super Signal West Dura Extended Duration Substrate (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... The blot was washed three times (15 mL PBS-T, 10 min, rt) and WGA was visualized using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Scientific).
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bioRxiv - Cell Biology 2021Quote: ... Blots stained with HRP-conjugated secondary antibodies were imaged using BioRad ChemiDoc imagers with chemiluminescence substrates: either SuperSignal West Pico PLUS (Thermo Scientific, 34577) or SuperSignal West Femto (Thermo Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Protein bands were visualized using a LI-COR Biosciences Odyssey FC imaging system after the addition of SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific). Proteins were quantified with Image Studio Version 5.2.4 (LI-COR Biosciences) ...
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bioRxiv - Molecular Biology 2022Quote: ... the membrane was washed two times with TBS-T and one time with TBS (TBS-T without Tween-20) before SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific 34077) was used for imaging ...
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bioRxiv - Immunology 2022Quote: ... a horseradish peroxidase (HRP)-labeled donkey anti-goat antibody (1:5000, Dinova) and Super Signal West Femto Chemiluminescence Substrate (Thermo Fisher Scientific). As a loading control ...
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bioRxiv - Microbiology 2022Quote: ... secondary antibodies (Kirkegaard & Perry Laboratories, Gaithersburg, MD) were used and visualized by SuperSignal West Dura chemiluminescent substrate or ECL (Thermo Fisher Scientific). All Western blots were performed with at least three biological and technical replicates and significance determined by t-test analysis.
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bioRxiv - Biophysics 2022Quote: ... Membranes were then washed three times before being subjected to SuperSignal West Pico PLUS Chemiluminescent Substrate or Dura Extended Duration Substrate (Thermo Fisher Scientific) peroxide solution treatment for visualization of antibody-bound proteins using a Bio-Rad ChemiDoc Touch Imaging System.
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bioRxiv - Cancer Biology 2022Quote: ... incubated with HRP-conjugated secondary antibodies at room temperature for 1 h and visualized using enhanced chemiluminescence substrate (SuperSignal™ West Dura Extended Duration Substrate #34076, Thermo Scientific).
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bioRxiv - Cancer Biology 2019Quote: ... secondary antibodies were used at a concentration of 1:5000 and detected using SuperSignal West Dura Extended Duration Substrate (Thermo Scientific, 34076).
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bioRxiv - Immunology 2020Quote: ... Binding of the primary antibody was detected using peroxidase-conjugated secondary antibodies and a SuperSignal West Dura chemiluminescent substrate kit (Thermo Scientific, 34075) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The membrane stained with Clean-Blot™ IP detection reagent was treated with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (ThermoFisher 34577) for at least 5 min at room temperature before imaging ...
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bioRxiv - Plant Biology 2020Quote: ... The nitrocellulose membranes were washed three times for 15 minutes in TBST and imaged using Supersignal® West Femto Maximum Sensitivity Substrates (Thermo Scientific) and X-ray film ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were incubated overnight at 4°C in primary antibodies diluted in blocking buffer and after washing in PBST the blots were developed with the SuperSignal West Dura ECL kit (InVitrogen, Slangerup, DK). Primary antibodies were rabbit monoclonal anti-cleaved Notch1 (Val1744 ...
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bioRxiv - Molecular Biology 2020Quote: ... signals were detected using ImageQuant LAS400 (General Electric) and images were captured using a SuperSignal™ West Pico Plus Chemiluminescent Substrate (Thermo Scientific) or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Scientific).
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bioRxiv - Microbiology 2019Quote: ... The lysates were run on a 15% PAGE and developed using monoclonal anti-His antibody (Biospecs, BTL1010) with super signal west femto maximum sensitivity substrate (Thermo Scientific, 34095).
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bioRxiv - Genomics 2021Quote: ... Chemiluminescence for UCP1 and COX4 was detected with SuperSignal West Femto Maximum Sensitivity Substrate (cat no. 34095, Thermo Fisher Scientific, Rockford, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies and HRP-conjugated secondary antibodies and then blotted with substrates or Super Signal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific). Imaging was performed with the Image Lab software ...
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bioRxiv - Biochemistry 2021Quote: ... The transferrin-HRP or HRP-conjugated secondary Abs were measured either by luminescence using HRP-Substrate (SuperSignal West Pico, Thermo Fisher Scientific) or Ampilite (ATT Bioquest ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were washed five times with TBST and immunoreactivity was detected using SuperSignal West Pico PLUS Chemiluminescent substrate (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2020Quote: ... The membrane was washed for two minutes five times and incubated with chemiluminescent subtrate (SuperSignal™ WEST pico PLUS, Thermo Fisher Scientific) and imaged using a G:Box (Syngene) ...
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bioRxiv - Bioengineering 2021Quote: ... Primary antibodies were detected with peroxidase-conjugated anti-rabbit or anti-mouse IgG (1:3000) and visualized with SuperSignal West Femto Substrate (ThermoFisher Scientific #34094). Blots were quantifi ed using ImageJ (https://imagej.nih.gov/ij/).
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bioRxiv - Cell Biology 2020Quote: ... were used as secondary antibodies and Western blots were developed using the SuperSignal™ West Pico Plus Chemiluminescent Substrate (Thermo Fisher Scientific) and an Image Quant LAS 400 (GE Healthcare ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were washed an additional 3 times for 15min each in TBST and visualized after a 5min incubation with SuperSignal West Dura Extended Duration Substrate (Thermo Scientific, 34075), or SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific ...