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Citations for Thermo Fisher :
3451 - 3500 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Significant control of Zika infection in macaques depends on the elapsing time after dengue exposurebioRxiv - Immunology 2019Quote: Viral RNA for real-time PCR assay was extracted from 140 ml of virus isolate (previously tittered as described above) and serum samples using Invitrogen PureLink RNA Mini Kit (Invitrogen, Valencia, CA) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cell supernatants were harvested at 48 h post-infection and virus titers were determined by infecting TZM-bl cells and measuring β-galactosidase activity using Galacto-Lite system (Applied Biosystems). For flow cytometry quantification of infection ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight with either Dengue 2 fixed virus antigen (Microbix, Cat# EL-22-02) or polyvalent total immunoglobulin (Invitrogen, Cat# H17000). Before adding PBMC’s ...
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bioRxiv - Immunology 2021Quote: ... T2 target cells and Epstein-Barr Virus-transformed B lymphoblastoid cell lines (EBV-LCLs) cells were cultured in RPMI-1640 (Thermo Fisher Scientific) supplemented with 10% FCS and 1% penicillin/streptomycin (#P433 ...
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bioRxiv - Immunology 2020Quote: ... at a concentration to give 100 to 250 foci per well in the virus only control wells was mixed 50:50 in 1% FCS MEM with 1 x antibiotic/antimycotic (Gibco, 15240-062) with serum doubling dilutions from 1:20 to 1:640 (or higher dependent on antibody levels ...
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bioRxiv - Immunology 2020Quote: ... Virus heat inactivation at 56 °C for 30 minutes and total RNA including virus RNA extraction was performed using TRIzol™ Plus RNA Purification Kit (ThermoFisher, USA). cDNA synthesis was performed using GoScript™ Reverse Transcription System (Promega ...
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bioRxiv - Microbiology 2021Quote: ... RNA extracted from animal samples or RNA standards were then measured using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, catalog # 4444432) and custom primers/probes targeting the E gene sgRNA (forward primer ...
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bioRxiv - Genetics 2022Quote: ... 30 μg of the virus) was digested with 1:100 (w/w) ratios of trypsin (Pierce Trypsin Protease mass spectrometry grade; Thermo Fisher Scientific)/virus for various time points at 25°C (31 ...
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bioRxiv - Evolutionary Biology 2022Quote: Virus RNA from nasal swabs was extracted from 50 µL of supernatant using the MagMAX-96 AI/ND Virus RNA Isolation Kit (Ambion, Austin, TX) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Reverse transcription (RT) of the extracted RNA samples (1 µg) was performed using AMV (Avian Myeloblastosis Virus) Reverse Transcription Kit (Invitrogen, Waltham, USA) and oligo-dT18 (1 µM ...
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bioRxiv - Microbiology 2023Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The first-strand cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), using 100 ng of purified RNA ...
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bioRxiv - Microbiology 2023Quote: ... the first-strand human cDNA was obtained using oligo(dT) primers and Moloney murine leukemia virus-reverse transcriptase (MMLV-RT kit; Life Technologies, USA), and The SuperScript™ VILO™ cDNA Synthesis Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... The virus supernatant was collected after 24 hrs and 48 hrs and filtered with a 0.45-mm syringe filter (Thermo Fisher, CA, USA). pLenti-shCtrl (negative silencing control ...
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bioRxiv - Neuroscience 2023Quote: ... from the Stanford Gene Vector and Virus core unless otherwise specified) and Ctb retrograde tracers (1 ug/ul; Invitrogen #C34777, #C34776, #C34775) were infused with a syringe pump (Harvard Apparatus ...
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Enhancing gene transfer to renal tubules and podocytes by context-dependent selection of AAV capsidsbioRxiv - Cell Biology 2023Quote: ... One μg DNA or 4 μL cDNA was used to PCR-amplify virus barcode (VBC) using Platinum SuperFi II DNA Polymerase (12361010, Invitrogen, Waltham, MA). Information on primer sequence including frameshifting nucleotide ...
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bioRxiv - Microbiology 2023Quote: ... The pooled libraries were mixed with virus-specific 161 biotinylated probes in the presence of human Cot-1 DNA (Invitrogen, Cat#15279011) and xGen Universal Blocking Oligos (IDT ...
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bioRxiv - Cancer Biology 2022Quote: ... Culture medium was replaced with lentivirus-containing medium at the ratio of 100 μL virus-containing medium per 25,000 cells seeded in the presence of 6 μg/mL polybrene (Thermo Fisher Scientific #TR1003G), followed by centrifugation at 2,000 rpm in room temperature for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... These plasmids were co-transfected with MLV (murine leukemia virus)-CMV (cytomegalovirus) luciferase and MLV Gag/Pol plasmids into HEK-293T cells (ATCC CRL-3216) using Lipofectamine 2000 (Thermo Fisher 11668019) transfection reagent following the manufacturer’s recommended protocol ...
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bioRxiv - Immunology 2022Quote: ... and subjected to the one-step quantitative real-time reverse transcription-PCR assay (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (ThermoFisher, Cat# 4444432) as described previously58 ...
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bioRxiv - Microbiology 2023Quote: ... virus was serially diluted in Vero maintenance media (MEM supplemented with 2% FBS and 1% each of GlutaMax (Gibco, Grand Island, NY), PenStrep ...
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bioRxiv - Microbiology 2023Quote: ... First-strand cDNA synthesis was performed on 1 µg of total RNA with the RevertAid H Minus Moloney murine leukemia virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific) using random primers p(dN)6 (Roche) ...
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bioRxiv - Immunology 2023Quote: ... The next day cells were infected with VSV-GFP virus at multiplicity of infection (MOI) of 1 in serum-free DMEM (DMEM Thermo Fisher, 11965092). After 1hr of incubation with media containing virus ...
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bioRxiv - Cell Biology 2023Quote: ... The virus supernatant was collected after 24 hrs and 48 hrs and filtered with a 0.45-μm syringe filter (Thermo Fisher, CA, USA); polyethylene glycol (PEG ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We added 50 larvae that have orally ingested half their body lengths of virus solution to 2 25-cell compartmentalized square petri dishes (ThermoFisher Scientific, U.S.A.) with ‘standard’ food and incubated them for 20 days [38] ...
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bioRxiv - Plant Biology 2024Quote: ... 1.0 μg DNase-treated RNA was reverse transcribed in a reaction volume of 20 μl containing oligo-(dT)19 primer and Moloney murine leukemia virus reverse transcriptase (Thermo Fisher Scientific). RT-qPCR analysis was then performed with 1.0 μl of 5-fold diluted cDNA using a SYBR premixed Ex Taq kit (Takara) ...
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bioRxiv - Microbiology 2024Quote: ... and after one hour virus was removed and cells were washed in PBS once before replacing culture media (DMEM F-12 (Thermo Fisher Scientific), penicillin (100LIU/ml)/streptomycin (100Lμg/ml ...
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bioRxiv - Microbiology 2024Quote: ... levels quantitative real-time PCR (RT-qPCR) was performed as previously described22 using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher, #4444436) and an OneStepPlus Real-Time PCR System (96-well format ...
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bioRxiv - Biochemistry 2020Quote: ... Total protein quantification of the glass bead protein extracts was conducted using the BCA Protein Assay kit (Thermo Fisher), and samples with equal amount of proteins were applied on SDS-PAGE gels ...
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bioRxiv - Neuroscience 2020Quote: ... and pre-cleaned with protein A/G beads (Dynabeads™ Protein A, 10002D, Dynabeads™ Protein G, 10004D, Invitrogen). Immunoprecipitation was conducted with a mixture of Aβ antibodies (6E10 ...
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bioRxiv - Cell Biology 2020Quote: IPs with FLAG-tagged protein were performed with Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D) and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse ...
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bioRxiv - Microbiology 2021Quote: Purified ThsA protein was freshly prepared and protein concentration was quantified using Qubit Protein Assay Kit (ThermoFisher cat # Q33212). Protein was diluted in wash buffer to a concentration of 147 ng/μl ...
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bioRxiv - Microbiology 2021Quote: ... were used to pull-down FLAG-tagged proteins and any associated proteins rather than the Dynabead Protein G (Invitrogen) pre-bound with anti-FLAG M2 antibody (SigmaAldrich ...
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bioRxiv - Neuroscience 2019Quote: Membrane proteins and cytosolic proteins were isolated using Mem-PER™ Plus membrane protein extraction kit (Thermo fisher scientific) according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2019Quote: ... and total protein concentration was quantified using a BCA protein assay (Pierce BCA Protein Assay kit, 23225, ThermoFisher Scientific). Samples were diluted to 0.8 ug/uL with a 1X cell lysis buffer provided by the manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... Protein concentrations were measured using the Bio-Rad Protein Assay Kit or BCA Protein Assay Kit (Thermo Fisher Scientific). Equal amounts (25 μg or 10 μg ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the protein extract was then measured with a BCA Protein Assay Kit (Thermo Fisher Scientific). A volume of each protein extract corresponding to 14 mg protein was transferred into a new 5-mL tube containing 150 µL streptavidin beads (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: Protein extracts from cells were assessed for protein concentrations using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Equal amounts of protein lysates were added to SDS-PAGE gels and electrophoresis was performed ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the protein extract was determined using a BCA protein assay kit (CAT# 23225, Thermo Fisher Scientific) and adjusted to 500 ng ...
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bioRxiv - Biochemistry 2023Quote: ... Protein concentration in the protein extract was determined using a BCA protein assay kit (CAT# 23225, Thermo Fisher Scientific) and adjusted to 2 μg/μL with the IP lysis buffer ...
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bioRxiv - Cell Biology 2023Quote: Protein concentrations were determined using the BCA protein assay (Micro BCATM protein assay kit; Thermo Fisher Scientific, MA, USA) and CLARIOstar microplate reader (BMG labtech Durham ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentration was quantified using the “Protein Quantification BCA Assay” kit (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2019Quote: Proteins were isolated using RIPA buffer and protein concentrations were determined using the BCA Protein Assay Kit (Thermo Fisher Scientific). Total RNA was extracted using RNeasy Mini Kit (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentrations were measured using a protein assay kit (Pierce™ BCA Protein Assay Kit, #23225, ThermoFisher Scientific, Cheshire, UK). Protein samples were resolved through 10% SDS-polyacrylamide gel and transferred to nitrocellulose membrane ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant containing proteins was collected and the protein concentration was determined by Pierce BCA Protein Assay Kit (Thermo Scientific). Immunoblotting analysis was carried out as previously described.(8 ...
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bioRxiv - Cancer Biology 2020Quote: ... Protein extracts were eluted in 2% SDS and protein concentration was measured using MicroBCA Protein Assay Kit (Thermo Fisher Scientific). Peptide isolations were digested by trypsin and SDS was removed employing Detergent Removal Spin Columns (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2022Quote: In vitro sumoylation reactions were performed on protein array slides with more than 9000 human proteins spotted (ProtoArray® Human Protein Microarray v5.0, Invitrogen). The reaction was performed by the technical service of Invitrogen with our purified enzymes ...
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bioRxiv - Molecular Biology 2020Quote: ... following which protein-protein complexes were purified with Pierce(tm) His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, #21277) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentrations were measured using the BCA Protein Assay Kit (Pierce™ BCA Protein Assay kit, Thermo Fisher Scientific, 23225) and a Tecan Infinite M200 Plate Reader.
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bioRxiv - Biochemistry 2021Quote: ... Protein concentration was measured through the Bicinchoninic Acid protein assay (Pierce™ BCA Protein Assay Kit, Thermo Scientific™ 23225). Equal amounts of proteins extracts (20-30 μg for mitochondrial samples or 40 μg for total kidney homogenates ...