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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Proteins were quantified by Pierce™ BCA Protein Assay (Thermo Scientific). Equal amounts of proteins (50μg ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein lysates were quantified using Qubit Protein Assay Kit (Invitrogen #Q33211) and mixed with the western blot denaturing loading dye (125mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... and protein was extracted with tissue extraction protein reagent (ThermoFisher Scientific) mixed with phosphatase and protease inhibitor (Sigma-Aldrich) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The resultant sgRNAs and Cas9 protein (TrueCut Cas9 Protein v2, Invitrogen) were diluted in RNase-free water to final concentrations of 500 and 600 ng/µL ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein concentrations were determined by BCA protein assay (Thermo Scientific; 23227). 20 μg of protein were loaded to SDS polyacrylamide gel electrophoresis and proteins were transferred to PVDF membrane using standard wet transfer method (80 volt ...
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bioRxiv - Immunology 2023Quote: ... and the protein concentration determined using EZQ Protein Quantitation Kit (Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... Protein concentrations were determined using the BCA protein assay (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein content was quantified with Pierce BCA Protein Assay Kit (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Protein concentration was determined using Coomassie protein assay (Thermo Scientific, #23200). Samples were precleared ...
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bioRxiv - Genomics 2023Quote: ... coupled with 40 μl protein A and protein G beads (Invitrogen) at 4 degrees Celsius overnight ...
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bioRxiv - Zoology 2023Quote: ... Protein concentration was determined by BCA protein assay (Thermo Fisher Scientific) with BSA standards and proteins analysed by reducing SDS-PAGE gel.
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bioRxiv - Plant Biology 2023Quote: ... 50μL of Protein A and 50μL of Protein G Dynabeads (Invitrogen) were combined ...
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bioRxiv - Developmental Biology 2024Quote: ... protein concentrations were determined by Bradford protein assay (Thermo Fisher Scientific). Equal amounts of lysates were loaded for individual experiments and β-tubulin was immunoblotted as the loading control ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined using BCA protein assay Kit (ThermoFisher Scientific). The non-reactive human monoclonal control AAB (mCtrl ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were quantified with the Qubit Protein Assay Kit (Invitrogen, Q33212), dilute to 1 µg/µl followed by denaturation using 5 X SDS loading buffer (CWBIO ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was quantified using a BCA protein assay (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations were quantified using Pierce BCA Protein Assay Kit (ThermoFisher) on an Infinite M200 plate reader (Tecan) ...
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bioRxiv - Cancer Biology 2023Quote: ... and protein amount was analyzed by BCA protein assay (ThermoFisher Scientific). Protein was denatured by adding NuPAGE LDS buffer and DTT reducing buffer (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... After protein quantification (Pierce BCA Protein Assay Kit, Thermo Fisher Scientific), 15 micrograms of total protein were separated using stain-free 4–15% gradient Tris-glycine SDS-polyacrylamide gels (Mini PROTEAN® TGX Stain-Free™ Gels ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated on 4-12% Bis-Tris Protein gels (Invitrogen) and then horizontally transferred to the Immobilon-FL PVDF membrane (#IPFL00010 ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined using BCA protein assay kit (Thermo Fisher). 10 µg of protein with 2X loading dye (without β-mercapto-ethanol and no heating of the sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentrations were determined using BCA protein assay kit (Thermo Fisher). 30-40 µg of protein with SDS loading dye (4x ...
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bioRxiv - Immunology 2023Quote: ... Protein concentrations were determined by a BCA Protein assay (Thermo Fisher).
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was assessed using BCA protein assay (Thermo Fisher Scientific). Protein electrophoresis was performed using 4–15% Mini-PROTEAN TGX Precast acrylamide gel (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... 100 μl Dynabeads Protein A and Protein G (Thermo Fisher Scientific) were pre-incubated overnight with rotation in the presence of 5 mg/ml BSA and 10 μg antibody (MYCN (B8.4.B) ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentration was determined with the Qubit protein assay (ThermoFisher Scientific) following manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Protein concentrations were quantified using BCA Protein Assay Kit (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Protein concentration was measured using BCA Protein Assay (Fisher Scientific, PI23227). 20 µg of protein was removed for total protein measurements ...
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bioRxiv - Neuroscience 2024Quote: ... Protein estimation was carried out using BCA protein estimation kit (Invitrogen).
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentration was measured by BCA protein assay (Thermo Fisher Scientific). Fifteen to thirty micrograms of lysates were separated on 4-15% precast Mini-PROTEAN® TGX™ gels (Bio Rad ...
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bioRxiv - Plant Biology 2024Quote: ... Protein concentration then determined using a Qubit protein assay kit (Invitrogen). The MUG fluorescent assay was performed in duplicates with 20 µl protein extract in MUG assay buffer (50mM phosphate buffer (pH 7.0) ...
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bioRxiv - Microbiology 2021Quote: ... Viral RNA was measured by quantitative real real-time polymerase chain reaction (qRT-PCR) using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with primers and probe specific for the CCHFV L gene.
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bioRxiv - Evolutionary Biology 2021Quote: ... we harvested virus from each virus line under sterile conditions by collecting up to 10 virus killed cadavers per line and transferring these to sterile 15 mL disposable tissue grinders (ThermoFisher Scientific, U.S.A.). Infected larvae were recognizable by their opaque ...
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bioRxiv - Immunology 2021Quote: ... Total mRNA was extracted from 190 pMHC-tetramerpos purified (Supplementary Figure 1A) virus-specific T-cell populations(37) using magnetic beads (Dynabead mRNA DIRECT kit; Invitrogen, Thermo Fisher Scientific). Ten μl (~1μg ...
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bioRxiv - Evolutionary Biology 2019Quote: ... A common virus stock was generated by transfection of plasmid DNA into NLFK cell culture using Lipofectamine 2000 (Life Technologies, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... ZHBTc4 cells were transduced with pLV-pCAGGS-Tir1-V5 and PLEX-mCherry-OCT4-AID packaged lentiviral vectors (i.e Tir1-V5 and mCherry-Oct4-AID virus, respectively) and were selected with 2 μg/ml Puromycin (Thermo Fisher Scientific #A1113803) for 10 days ...
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bioRxiv - Microbiology 2020Quote: Proteins were harvested four to five days post virus infection in RIPA Lysis and Extraction Buffer (Boston Bio) with 1X Protease Inhibitor and 1X Phosphatase Inhibitor (Thermo Fisher Scientific). Protein Concentration was determined using BCA Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... cells (in-house stock regularly tested to be virus- and mycoplasma-free) were grown at 25°C in S2 cell media supplemented with 10% fetal bovine serum (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2021Quote: ... the extracellular RSV virus were collected by PEG6000 precipitation method and intracellular RSV virus were collected by lysing the macrophage cells with the lysis buffer from mirVanaTM miRNA Isolation Kit (Ambion, Life Technologies, AM1560). Total RNA from RSV infected macrophage cells as well as stock virus was extracted using mirVanaTM miRNA Isolation Kit according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Purity and titer of virus were then assessed by SDS-PAGE and SYPRO ruby staining (S-12000, Life technologies, Carlsbad, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... For the preparation of the BGLF2-KO virus, HEK293/EBV(dBGLF2) cells (Konishi et al., 2018) were transfected with BZLF1 expression plasmid using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and quantitative real-time polymerase chain reaction (qRT-PCR) was conducted using TaqMan Fast Virus 1-Step Master Mix (Thermofisher Scientific, US) with primers and probe specific for the SARS-CoV-2 E gene following guidelines by the World Health Organization (https://www.who.int/docs/default-source/coronaviruse/wuhan-virus-assayv1991527e5122341d99287a1b17c111902.pdf ...
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bioRxiv - Microbiology 2021Quote: ... HCV RNA was quantified by one-step reverse transcription-quantitative PCR using 50 ng of total intracellular RNA and TaqMan® Fast Virus 1-Step Master Mix (Applied Biosystems) with primers and probe targeting the HCV 5’ nontranslated region as described previously [82] ...
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bioRxiv - Microbiology 2022Quote: The ZEBOV RT-PCR assay was performed in a 25 µL reaction mixture containing TaqMan Fast Virus 1-step master mix (Thermo Fisher Scientific), 5 µl template RNA ...
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bioRxiv - Microbiology 2019Quote: ... total RNA was reverse transcribed using virus-specific RT primers or oligo-dT primers and the SuperScript III First-Strand Synthesis System (Invitrogen, Carlsbad, CA). Real-time quantitative PCR was performed on the Applied Biosystems 7500 system with QuantiTect SYBR Green PCR Master Mix (Qiagen ...
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bioRxiv - Neuroscience 2019Quote: cDNA sequences from the leader through the first half of the rabies virus P gene were amplified using Platinum SuperFi Green Master Mix (Invitrogen (Thermo Fisher), USA ...
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bioRxiv - Microbiology 2020Quote: ... apical poles were gently washed with warm PBS and then infected with a 150-μL dilution of HRSV-A (Long) virus in OptiMEM medium (Gibco, ThermoFisher Scientific) at a MOI of 1 ...
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bioRxiv - Microbiology 2021Quote: Recombinant B19 virus-like particles (VLPs) consisting of VP2 were produced using the ExpiSf™ Expression System Starter Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... One microgram of RNA was reverse transcribed into cDNA using oligo-dT and MMLV-RT (Moloney Murine Leukemia Virus Reverse Transcriptase, Invitrogen, Thermo Fisher Scientific). Before cDNA synthesis ...
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bioRxiv - Neuroscience 2019Quote: ... Patient KS1-1 was consented for stem cell derivation and cells were reprogrammed using non-integrating Sendai virus vectors (CytoTune-iPS 2.0, ThermoFisher Scientific, Waltham, MA). 5 days post-induction ...