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Citations for Thermo Fisher :
3501 - 3550 of 10000+ citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... For glutamine starvation experiments cells were washed once with PBS and incubated for 6 hours in DMEM/F12 without glutamine and supplemented with 10% (v/v) dialyzed FBS (Gibco), 1% (v/v ...
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bioRxiv - Plant Biology 2020Quote: ... the membrane was washed 4 times with 1X TBS containing 0.1% (v/v) Tween-20 and incubated with 1/5,000 diluted goat anti-rabbit horseradish peroxidase-conjugated secondary antibody (Thermo Scientific) in 5% milk TBS for 1 hour at room temperature with shaking ...
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bioRxiv - Cell Biology 2021Quote: ... BSF RNAi cell lines were cultured in HMI-9 medium 47 supplemented with 10% (v/v) low-tetracycline foetal calf serum (FCS; Gibco) at 37 °C in 5% C02 and maintained in 5 µg.mL-1 Hygromycin (InvivoGen ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 10% v/v heat-inactivated fetal bovine serum (HyClone 12483-020) 1% v/v penicillin 10,000 Unit/ml streptomycin 10,000 μg/ml (Gibco 15140-163) and incubated at 37 °C in a humidified atmosphere containing 5% CO2.
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bioRxiv - Immunology 2021Quote: ... 5e3 VeroE6 cells were plated per well in DMEM (Quality Biological) supplemented with 10% v/v fetal bovine serum (Gibco), 1% v/v Penicillin-Streptomycin (Gemini-Bio) ...
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bioRxiv - Biophysics 2021Quote: ... at 37 °C in a 5% (v/v) CO2 humidified environment (Forma Steri-Cycle CO2 incubator, Thermo Fisher Scientific, Singapore).
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bioRxiv - Biophysics 2022Quote: ... at a concentration of 2×105 cells/ml (2 mL per well hereafter) in DMEM (Nissui Pharmaceutical) supplemented with 10% (v/v) FBS (Gibco), glutamine ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... supplemented with 10% (v/v) fetal bovine serum (FBS, HyClone) and penicillin (100 U/ml)/streptomycin (100 μg/ml) (Gibco) in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Genetics 2020Quote: ... the CRISPR-Cas9 ribonucleoprotein complexes were encapsulated in liposomes for 20 minutes at room temperature (3% v/v RNAIMAX (Invitrogen) or CRISPRMAX (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... Discs were transferred to hybridization buffer (10% w/v dextran sulfate, 4X SSC, 0.01% w/v salmon sperm ssDNA (Invitrogen 15632), 1% v/v vanadyl ribonucleoside (NEB S14025) ...
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bioRxiv - Cell Biology 2021Quote: ... 1% [v/v] FCS in PBS++) and primary antibodies were detected with secondary antibodies conjugated to Alexa Fluor dyes (Invitrogen). Images were acquired as above ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and then treated with compounds in media containing 10% FBS (v/v) but without the dexamethasone recommended by Life Technologies.
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bioRxiv - Pharmacology and Toxicology 2020Quote: The J774.A1 murine macrophage cells were grown in Roswell Park Memorial Institute (RPMI) 1640 Medium in the presence of 10% (v/v) FBS and penicillin-streptomycin (Gibco, Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dissolved in 300 μl of 6:1 (v/v) of methyl sulphoxide D6 (99.9% atom D + 1% tetramethylsilane, ACROS Organics):D2O (100% atom D ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 μg/ml streptomycin (1% p/s) and 10% (v/v) Fetal Bovine Serum (FBS) (all Invitrogen, Bleiswijk, The Netherlands). Cells were passaged at confluence for maintenance and collected and lysed using TRIzol reagent (Ambion by Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were eluted with 0.1% (v/v) trifluoroacetic acid (TFA) and dried in a SpeedVac system (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated for 30 minutes with Mitotracker Green FM or Mitotracker Red (1:1000 v/v dilution of 1 mM stock) according to manufacturer’s instructions (Invitrogen Molecular Probes), in complete media at 37°C in the dark ...
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were resuspended in lysis buffer (high or low salt buffer, with 1% v/v Triton X-100, 400 units/ml RNAseOUT (Invitrogen), 100 µg/mL CHX ...
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bioRxiv - Neuroscience 2021Quote: ... HEK293T cells were cultured at 37°C in 5% CO2 in DMEM (Biological Industries) supplemented with 10% (v/v) fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin (Biological Industries) ...
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bioRxiv - Biochemistry 2021Quote: ... One part of the transfection complexes was then mixed with 20 parts (v/v) of HEK293T cells suspended at a density of 4×105 in DMEM (Gibco). Cells were seeded into white ...
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bioRxiv - Biochemistry 2021Quote: ... cultured in complete DMEM and RPMI medium i.e supplemented with 10 % (v/v) FBS (cat. no. 10270-098, Gibco, ThermoFisher Scientific), 2 mM L-glutamine (cat ...
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bioRxiv - Microbiology 2020Quote: ... and 1% v/v Pen/Strep (100 U/ml of penicillin, 100 μg/ml of streptomycin, Gibco, Cat#: 15140-122). A549 cells (Cat# ...
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bioRxiv - Neuroscience 2020Quote: ... C2C12 cells were allowed to differentiate into myotubes in DMEM supplemented with 5% (v/v) horse serum (Gibco-Life Technologies) and 1% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... C2C12 cells were allowed to differentiate into myotubes in DMEM supplemented with 5% (v/v) horse serum (Gibco-Life Technologies) and 1% (v/v ...
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bioRxiv - Immunology 2020Quote: ... modified with 1% (v/v) each of minimum essential media (MEM) non-essential amino acids (Gibco; Grand Island, NY, USA), sodium pyruvate (Gibco ...
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bioRxiv - Immunology 2021Quote: ... 38.4% (v/v) acetonitrile over 30 min with column effluent directed into an Orbitrap Elite mass spectrometer (Thermo-Fisher Scientific). Data were acquired in both data-dependent MS:MS mode and MS1 profile mode ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was then incubated overnight at 4°C and under agitation with 2 mL Ni-NTA resin (50% v/v, Thermofisher) pre-equilibrated with lysis buffer.
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bioRxiv - Immunology 2020Quote: ... were maintained in monolayer in 150cm2 tissue culture flask in DMEM supplemented with fetal bovine serum (FBS, 10% v/v, Invitrogen) and penicillin-streptomycin/amphotericin B (1% v/v) ...
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bioRxiv - Immunology 2020Quote: ... Media in the wells seeded with Vero E6 was replaced with 100 µL DMEM with 10% (v/v) FBS and 1% Antibiotic-Antimycotic (Gibco) and 100 µL of the sera-virus mixture was added into the wells ...
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bioRxiv - Immunology 2021Quote: ... or in sterile Sorting Medium [RPMI 1640 supplemented with 10% (v/v) Heat-Inactivated Fetal Bovine Serum (HI-FCS; A3840001; Gibco)] ...
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bioRxiv - Immunology 2021Quote: ... USA) were grown as a monolayer in DMEM10 (Dulbecco’s minimal essential medium, DMEM, Life Technologies, supplemented with 10% (v/v) hiFCS ...
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bioRxiv - Cell Biology 2021Quote: ... and 8 mM MgSO4 pH 7.0 (2x PHEM buffer) containing 1% Triton X100 for 5 min and then fixed in 4% (v/v) paraformaldehyde (ThermoFisher) in 2x PHEM buffer at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... supplemented with 100 U/mL penicillin and 100 µg/mL streptomycin and containing 10% (v/v) heat-inactivated horse serum (#26050088, ThermoFisher). Cells were seeded at 2 × 105 cells/mL in 25 cm2 flasks (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Pluronic acid (Pluronic F-127, 20%(v/v) in DMSO) was purchased from Invitrogen (Carlsbad, CA, USA; Cat: P-3000MP). BAPTA-AM was purchased from Invitrogen (Cat ...
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bioRxiv - Synthetic Biology 2023Quote: ... as well as 3% v/v DMSO and 50% with Phire II Hot-Start PCR 2X mastermix (Thermo Scientific; F125L), and topped-up to 25 μL with ddH2O ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.075% (v/v) β-mercaptoethanol and 1 mM benzamidine] supplemented with one tablet of Pierce protease inhibitor cocktail (ThermoFisher Scientific) and lysed by sonication using a Sonics Vibracell instrument (1 s ON/3 s OFF ...
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bioRxiv - Biophysics 2023Quote: ... was cultured in a 25-cm2 flask using HyClone Dulbecco’s modified Eagle’s medium (DMEM) with high-glucose (SH30022.01, Cytiva) supplemented with 10% (v/v) fetal bovine serum (FBS, 10500064, Life Technologies), 100 units/ml penicillin and 100 µg/ml streptomycin (Pen-Strep ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were fixed with cold methanol (10 min at −20°C) and blocked for 30 min with 10% (v/v) fetal bovine serum (Gibco) in 1 x PBS at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and 100 µl of the homogenate were added to 1:3 v/v of TRIzol™ LS (Thermo Fisher Scientific) and incubated for 10 min at room temperature to inactivate the virus ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were washed 2 times with flow assay buffer and subsequently stained with 100 nM of M1-⍺FLAG-AlexaFluor488 and 1:500 (v/v) of Alexafluor 647 conjugated anti-V5 antibody (ThermoFisher) (for AT118-H or AT118-L ...
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bioRxiv - Molecular Biology 2023Quote: ... goat serum in 0.1% (v/v) Triton X-100/PBS for 15 min and incubated with anti-GFP (ThermoFisher, A10262) and anti-mitochondrial HSP60 (Abcam ...
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Engineering TALE-linked deaminases to facilitate precision adenine base editing in mitochondrial DNAbioRxiv - Molecular Biology 2023Quote: ... ATCC) and B16F10 (CRL-6475, ATCC) cells were grown in DMEM supplemented with 10% (v/v) bovine calf serum (Gibco) for NIH3T3 cells or 10% fetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... All cell lines were cultured at 37°C in 5% CO2 in DMEM (Biological Industries) supplemented with 10% (v/v) fetal bovine serum (GIBCO) and 1% penicillin-streptomycin (GIBCO).
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bioRxiv - Neuroscience 2022Quote: ... the cells were cultured at 37°C in humidified air containing 5% CO2 in DMEM (Biological Industries) supplemented with 10% (v/v) FBS (Gibco), penicillin (100 U/ml) ...
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bioRxiv - Bioengineering 2024Quote: ... the 70% Percoll suspension of tumor cells was injected below a layer of 37% v/v Percoll (4mL, 37% Percoll, and 1% 1× PBS in Phenol-free RPMI-1640) (Gibco) in a 15 mL conical ...
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bioRxiv - Bioengineering 2024Quote: ... Bone marrow cells collected from the femur were seeded at a density of 1 x 106 cells/mL in KR158 cell-conditioned culture media (50% v/v KR158 conditioned media + 50% RPMI-1640 (Gibco) + 10% FBS (Corning ...
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bioRxiv - Biochemistry 2024Quote: ... Unlabeled KBM7 cells were maintained in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% (v/v) FBS and 1x Pen-Strep (Gibco #15140-122). Unlabeled Jurkat cells were maintained in Roswell Park Memorial Institute (RPMI ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was then mixed with an equal volume of phenol:chloroform:isoamyl alcohol (25:24:1 v/v; ThermoFisher Scientific, 15593031), and centrifuged for 10 minutes ...
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bioRxiv - Evolutionary Biology 2024Quote: All Drosophila cells were maintained on either Shields and Sang M3 Insect Medium (MilliporeSigma S3652) or Schneider’s Insect Medium (MilliporeSigma S0146) supplemented with 10% v/v Fetal Bovine Serum (FBS, ThermoFisher A3160502). Cells were maintained in 4 mL of media in plug-seal T-25 flasks (Corning 430168 ...
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bioRxiv - Immunology 2024Quote: ... A single dose of 10 plaque forming unit (PFU) of PVM in 10 µl or the same volume of vehicle (10%, v/v, FCS in DMEM; Gibco) was administered via the intra-nasal (i.n. ...