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Citations for Thermo Fisher :
3751 - 3800 of 10000+ citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The cell line was then adapted to suspension culture and maintained in Expi293™ expression medium supplemented with 1% (v/v) penicillin-streptomycin and 1 μg ml-1 puromycin (Gibco).
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Immunology 2021Quote: ... or [4A] Ac-ASQARPSQR or [4Y] Ac-ASQYRPSQR (custom products from GL Biochem Shanghai) in 10% FBS (v/v) RPMI containing 1% penicillin/streptomycin (Life Technologies) at 37°C and 5% CO2 for the indicated time points.
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bioRxiv - Immunology 2021Quote: ... Cells were washed once and resuspended in phenol-free RPMI with 2% (v/v) human AB serum containing DAPI (ThermoFisher 62248). Single-color controls were performed with these same antibodies using BD CompBeads (BD Biosciences 552843) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were pelleted and re-suspended in 1 ml of PBS with antibiotics (1% (v/v) Penicillin/Streptomycin (Invitrogen 15140-122); 0.1% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... sections were washed 3 times for 10 min per wash in PBS containing 0.02% (v/v) Triton X-100 and then incubated for 1 hr at room temperature with Alexa-flour secondary antibodies (Molecular Probes) diluted to 4 µg/mL in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: Peptides were dissolved in 4 μL 0.2% (v/v) formic acid (FA) and injected into an Easy-nLC 1200 (Thermo Fisher Scientific). Peptides were separated on a 15-cm in-house packed column (360 μm OD × 75 μm ID ...
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bioRxiv - Cell Biology 2022Quote: ... The attached cells were washed with ice cold PBS twice and then crosslinked with 1% (v/v) formaldehyde without methanol (Thermo Scientific) in DMEM for exactly 20 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were embedded in a thin polyacrylamide gel and were then treated with a digestion buffer of 2% v/v sodium dodecyl sulfate (SDS; ThermoFisher, AM9823), 0.5% v/v Triton X-100 (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by 15 minutes at 37 °C in 2X SSC containing 10% v/v Formamide and DAPI (Thermo Fisher Scientific D1306). Finally ...
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bioRxiv - Microbiology 2023Quote: ... After heating, 500 μL of phenol:chloroform:isoamyl Alcohol (25:24:1, v/v) (Invitrogen™ UltraPure™ - Fisher Scientific 15-593-031) was added to the lysing tube and bead beating proceeded with four 40 second rounds on the FastPrep-24 instrument (MP Biomedical – Cat ...
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bioRxiv - Cell Biology 2023Quote: ... Cryopreserved cells were thawed and activated with anti-CD3/anti-CD28 TransAct (Miltenyi) 1:100 v/v in T cell expansion medium comprising OpTmizer media (Thermo Fisher) containing 2% GlutaMAX (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... All cell lines were cultured in the corresponding culture medium recommended by the suppliers supplemented with 1% (v/v) penicillin/streptomycin (Gibco, #15140122) and in a humidified incubator with 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: Tubular scaffolds were sterilized in a laminar flow cabinet by washing 3 times for 15 minutes in 5% penicillin/streptomycin (v/v, Gibco, Thermofisher Scientific) in phosphate buffered saline (PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 30% acetonitrile (v/v) was added to the digested protein and then labeled using a TMT isobaric mass tagging kit (ThermoFisher Scientific). The TMT reaction was performed for 1 h according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Bacteria cells and meropenem in liquid media are briefly vortexed before mounting on a 1% (v/v) UltraPure agarose pad (10 x 20 mm, Invitrogen US) in M9 minimal media (Gibco ...
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bioRxiv - Physiology 2023Quote: ... Samples were dried in a speedvac and reconstituted in 0.1% TFA (v/v) and quantified using Qubit (Thermo Fisher Scientific, USA). The labeling efficiency was checked using the MALDI-TOF mass spectrometer (Autoflex III ...
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bioRxiv - Bioengineering 2022Quote: ... were transferred to a clean 48 wells plate filled with 200 μL of 10 v/v % PrestoBlue reagent (A13262, Thermo Fisher) in the appropriate medium (mono or coculture ...
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bioRxiv - Cell Biology 2024Quote: ... was cultured in Dulbecco’s Modified 305 Eagle’s Medium (DMEM) supplemented by 10% (v/v) fetal bovine serum and 100 units/ml 306 of penicillin-streptomycin (Cat# 15140122, Thermo Fisher). The dish was placed in a stage-CO2 incubator on a 3D Cell Explorer-fluo light microscope (Nanolive) ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1% [v/v] TFA and injected onto a C18 Pep-Map100-trapping column (Thermo Scientific, 0.3 x 5 mm, 5 μm) connected to an in-house packed C18 analytical column (Dr Maisch GmbH ...
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bioRxiv - Physiology 2024Quote: ... were used to perform buffer exchange (Phosphate-buffered saline [PBS] [Gibco, #14190], 50 mM NaCl [Sigma-Aldrich, #S5150-1L], 0.001 %, Pluronic F68 [v/v] [Gibco, #24040]) and the final concentration step ...
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bioRxiv - Cell Biology 2024Quote: ... to form condensates (with 20% v/v PEG 8 kDa as needed) and overlaid with fluorescent dextrans of various sizes at 10 kDa (Invitrogen D1816), 40 kDa (Invitrogen D1842) ...
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bioRxiv - Cell Biology 2024Quote: ... were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented by 10% (v/v) fetal bovine serum and 100 units/ml of penicillin-streptomycin (Cat# 15140122, Thermo Fisher). Cell subculture was performed twice a week to maintain cell viability and proliferation ...
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bioRxiv - Neuroscience 2023Quote: Lumbar spinal cord and hippocampal sections were rinsed in 0.1M PB and subsequently incubated at room temperature for 1h with 30% (v/v) normal goat serum (Invitrogen, Cat#31872) in 0.1M PB containing 10% Triton X-100 ...
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bioRxiv - Immunology 2023Quote: All cells were maintained in complete RPMI (RPMI-1640 supplemented with 2 mM glutamine, 10% [V/V] FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin; all Gibco/ThermoFisher) at 37°C and 5% CO2.
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bioRxiv - Cell Biology 2023Quote: ... followed by the addition of 15 μl of a 1:1 v/v methanol/acetonitrile mix (Fisher Scientific A456 and A955). The samples were then centrifuged at 20,000g at 4°C for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... cell pellets corresponding to two wells of a 6-well plate (double-well pellet with same treatment conditions) were lysed in 1% (v/v) TritonX-100 buffer (Fisher Scientific) in DPBS supplemented with 1% (v/v ...
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bioRxiv - Plant Biology 2024Quote: ... pyridoxine hydrochloride + 0.01% (w/v) thiamine hydrochloride + 0.0002% (w/v) glycine + 175 nm N6-Benzyladenine + 10 μM EdU (Invitrogen A10044, Thermo Fisher Scientific) pH 5.7] containing 0.8% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... a 1X dilution in DPBS of 10X neutral red stain (0.85% w/v NaCl, 0.5% w/v Fisher Scientific #N129-25) was applied to each well ...
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bioRxiv - Microbiology 2022Quote: ... yeast extract 0.75% (w/v) and glucose 1.5% (w/v)] in T75 Nunclon® tissue culture flasks (ThermoFisher Scientific, Loughborough, UK) at 25°C in a humidified Stuart™ SI30H Hybridization bench top oven/shaker (Cole-Parmer Ltd ...
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bioRxiv - Biophysics 2022Quote: ... Grids were plunge-frozen into a 37:63 (v/v) mixture of ethane/propane cooled to liquid nitrogen temperature using a Vitrobot Mark IV instrument (ThermoFisher Scientific) at 95% humidity and 4 °C ...
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bioRxiv - Bioengineering 2022Quote: ... the suspending fluid was 0.6% w/v or 0.7% w/v methyl cellulose (Thermo Scientific, molecular weight 454.513 g mol−1) in phosphate buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: FA-patient derived OHSU-SCC-974 and sporadic JHU-SCC-FaDu and UM-SCC-47 cell lines were grown in Eagle’s Minimum Essential Media (MEM) supplemented with 10% v/v FBS (Hyclone Laboratories, South Logan Utah) and 1% penicillin-streptomycin (Gibco #10378016). Sporadic cell line SFCI-SCC-9 was grown in a 1:1 mixture of Dulbecco-Modified Eagle’s Medium (D-MEM ...
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bioRxiv - Biochemistry 2022Quote: ... Each dried aliquot of the non-polar layer was reconstituted in 50 μl of methanol: toluene (9:1, v/v) (Fisher Scientific) and transferred to 2 mL LC-MS amber vials with 300ul glass inserts ...
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bioRxiv - Biophysics 2022Quote: ... The medium contains L-glutamine and sodium pyruvate and is supplemented with 10% (v/v) fetal bovine serum (FBS, ThermoFisher 10500064) and 1% penicillin-streptomycin (Gibco 15140122) ...
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bioRxiv - Microbiology 2022Quote: ... USA) supplemented with 10% fetal bovine serum (S0250, BioWest, France) and 1% (v/v) penicillin and streptomycin (15140-122, Gibco, USA).
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bioRxiv - Microbiology 2022Quote: ... were then separated by electrophoresis with either a 12% (v/v) SDS-acrylamide gel or a 4-12% gradient gel (Thermofisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Sections were stained for axons for 1 hr at room temperature with fluoromyelin (1:250 v/v in mammalian physiological saline; Invitrogen, F34651). These were then mounted onto 35 mm plastic dishes (Z707651 ...
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bioRxiv - Physiology 2022Quote: ... Triton-X in PBS for three minutes followed by blocking with blocking buffer (1% w/v bovine serum albumin with 0.2% w/v keyhole limpet hemocyanin in PBS; Thermo Fisher Scientific) for 30 minutes at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... For each replicate 150 wandering third instar larvae were collected and their brains were dissected in brain culture media (BCM: 80% v/v Schneider’s Drosophila medium (Thermo Fisher 21720024), 20% v/v heat inactivated fetal bovine serum (Sigma F4135 ...
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bioRxiv - Biochemistry 2022Quote: ... or 700 µL (30% (v/v) FBS condition) of FBS-free media per well in a 12-well plate (#55428, Nunc/DDBioLab) and starved at least 14h at 23°C ...
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bioRxiv - Cancer Biology 2023Quote: ... All media were supplemented with 10% fetal bovine serum (FBS) and 1% v/v penicillin/streptomycin (ref 15140122, GIBCO, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then spun down and resuspended in an extracellular matrix (ECM)-rich mixture containing 40% DMEM (%v/v, Gibco, 11995073), 40% fibrinogen (10 mg/mL stock concentration in a 0.9% NaCl water solution ...
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bioRxiv - Cell Biology 2023Quote: ... Cas9-transfected U-2 OS cells (1500 cells per well of the 384-well plate) were reverse transfected with crRNAs with 1% (v/v) RNAiMAX (Thermo Fisher) in serum-free media dispensed via a Multidrop combi ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were redissolved in 100 µl of chloroform/ methanol (1:1, v/v) and infused into an LTQ Orbitrap Velos Pro mass spectrometer (Thermo Scientific) using static infusion nanoflow probe tips (M956232AD1-S ...
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bioRxiv - Systems Biology 2023Quote: ... Blocking and permeabilization were performed for 1 h in 1 mL blocking buffer - Dulbecco’s phosphate buffered saline (DPBS) containing 5% (v/v) normal goat serum (Thermo Fisher Scientific) and 0.3% (v/v ...
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bioRxiv - Systems Biology 2023Quote: ... were maintained in Dulbecco’s Modified Eagle’s Media (DMEM, 4500 mg/L glucose, MilliporeSigma) supplemented with 10% (v/v) fetal bovine serum (FBS; Thermo Fisher Scientific). MCF-7 cells tagged with mVenus fluorescent protein at the endogenous p21 locus and transfected with DHB-mCherry and H2B-mTurquoise (a kind gift from Dr ...
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bioRxiv - Neuroscience 2023Quote: ... which include cell separation by gradient centrifugation and lymphocyte growth enhancement with 1% (v/v) of the mitogenic phytohemagglutinin-M (PHA-M, ThermoFisher 10576015).
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bioRxiv - Bioengineering 2023Quote: ... 75 and Thy1- eGFP mice of either sex as previously described.76 Incubation medium (pH = 7.38) contained 50% (v/v) minimum essential media (#21575 − 022, Thermo Fisher, USA), 25% (v/v ...
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bioRxiv - Physiology 2024Quote: ... were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (FBS; both from GIBCO, Carlsbad, CA, USA), and 0.1% penicillin-streptomycin (Sigma-Aldrich) ...