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Citations for Thermo Fisher :
3351 - 3400 of 10000+ citations for Annexin V Solution APC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 9.46 mM EDTA, 0.946% [v./v.] SDS, 0.01 mg/mL yeast tRNA, 1 mg/mL Proteinase K [Invitrogen, 25530049]) at 65°C with shaking at 1,200 rpm for 45 min ...
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bioRxiv - Biochemistry 2023Quote: ... supernatant was removed and cells were permeabilised with the addition of 1 ml 0.1% (v/v) Triton-X100 in PBS with Phosphatase Inhibitor Cocktail I and Protease Inhibitor Cocktail III (ThermoFisher) for 10 min on ice ...
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bioRxiv - Genomics 2023Quote: ... Cells were blocked for 1-2 hrs using 2% (v/v) Bovine Serum Albumin (BSA, Thermo Scientific, Cat # 37525) + 5% FBS in HBSS ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.01% [v/v] 2-mercaptoethanol) and then quantified with the Pierce BCA protein assay kit (Thermo Fisher Scientific). The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were grown in complete medium consisting of Dulbecco’s Modified Essential Medium with 10% (v/v) fetal bovine serum (FBS) (Gibco) and 1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... cells were mixed 1:1 (v/v) with the master mix for electroporation using a Neon Transfection System (Invitrogen; pulse voltage = 1600 ...
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bioRxiv - Cancer Biology 2024Quote: ... The final concentration was adjusted by adding at least 30% v/v Candor Antibody Stabilizer (Thermo Fisher Scientific, NC0414486) with additional 0.2% sodium azide ...
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bioRxiv - Genomics 2022Quote: ... and lysed in Lysis Buffer (PBS containing 1% (v/v) Triton X-100 and 25 U/mL TurboDNase (Ambion)) ...
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bioRxiv - Molecular Biology 2022Quote: ... for 10 min followed by permeabilization with in PBS with 0.1% v/v Triton-X-100 (PBS-TX) (ThermoFisher), blocked for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were treated with 0.1% v/v fluorescence-labeled phalloidin (Alexa Fluor® 568 Phalloidin, Thermo Fisher Scientific) in PBS for 30 min at room temperature in dark ...
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bioRxiv - Microbiology 2022Quote: ... The membranes were washed 3X with 1X PBS containing 0.05% (v/v) Tween-20 and signal was detected using SuperSignal West Femto maximum sensitivity substrate (Thermofisher) and a Biorad Gel Doc Imaging System.
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% (v/v) heat-inactivated fetal bovine serum (Omega) and 100 U/ml penicillin/streptomycin (Gibco-BRL).
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bioRxiv - Immunology 2022Quote: ... PBMCs were suspended in freezing media (90% (v/v) foetal bovine serum (FBS; Gibco/Thermo Fischer Scientific, Waltham, MA) and 10% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... All media were supplemented with 10% fetal bovine serum (FBS) and 1% v/v penicillin/streptomycin (ref 15140122, GIBCO, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... On day 5 the culture medium was replaced with DMEM/F12 containing 1% N2 (v/v, Gibco, Cat#17502048), 1% GlutaMAX (v/v) ...
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bioRxiv - Developmental Biology 2023Quote: ... and 1% v/v Triton X-100 and supplemented with protease inhibitor tablets (Cat. No. A32955, Thermo Fisher Scientific). Protein concentrations were determined by BCA protein assay ...
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bioRxiv - Microbiology 2023Quote: ... AB+ heat inactivated serum with 0.5%(v/v) gentamycin per condition in 1 mL volumes in 24-well plates (Nunc). The plates were incubated at 37°C and with mixed gas (5% CO2 ...
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bioRxiv - Molecular Biology 2023Quote: ... U2OS cells were cultured in McCoy’s 5A media with 10% (v/v) heat-inactivated fetal calf serum (10-437-028, Gibco) and 1% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were washed 3X with PBS containing 0.05% (v/v) Tween-20 and signal was detected using SuperSignal West Femto maximum sensitivity substrate (Thermofisher) and a Biorad Gel Doc Imaging System.
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bioRxiv - Cancer Biology 2024Quote: ... containing 4.5g/l Glucose with L-Gln and Sodium Pyruvate supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco), Gluta-MaxTM-1 (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... The pellet was resuspended in PBS containing 1:1000 (v/v) 7-AAD viability dye (Invitrogen, catalog number A1310). 500 cells per well were sorted on a BD FACSAria III sorter using a 100-micron nozzle in 4-way purity precision mode into an Eppendorf twin.tec 384 LoBind plate containing 1µl of 0.2% DDM ...
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bioRxiv - Developmental Biology 2024Quote: ... Different concentrations of acrylamide and bis-acrylamide were mixed to produce gels of different rigidity53 and mixed with 0.04% v/v fluorescent carboxylated 200 nm beads (Invitrogen), 0.05% APS (Sigma-Aldrich A3678) ...
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bioRxiv - Plant Biology 2024Quote: ... USA) and eluted using a 25-minute gradient comprising 0.1 % (v/v) formic acid (Fisher Scientific, Hampton, NH, USA) in LC-MS grade water (solvent A ...
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bioRxiv - Molecular Biology 2024Quote: ... Immerse the uterus into 75% (v/v) ethanol followed by rinsing with PBS without Ca2+ and Mg2+ (Gibco, Invitrogen). Separate each embryo from its placenta ...
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bioRxiv - Molecular Biology 2024Quote: ... Immerse the uterus into 75% (v/v) ethanol followed by rinsing with PBS without Ca2+ and Mg2+ (Gibco, Invitrogen). Separate each embryo from its placenta ...
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bioRxiv - Cell Biology 2024Quote: Exploris raw files were first split according to CVs (-45 V, -60 V) using FreeStyle 1.7 software (Thermo Scientific). The resulting split MS data were analyzed with FragPipe (19.1 or 20.0) ...
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bioRxiv - Microbiology 2024Quote: ... Cultures were maintained on blood agar base #2 (Oxoid) supplemented with 5 % (v/v) defibrinated horse blood (Thermo Scientific) and incubated under microaerobic conditions (5 % O2 ...
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bioRxiv - Immunology 2024Quote: ... 20mM tris buffer pH 8.0 and 0.5% v/v Nonidet P-40) supplemented with PierceTM Phospho-stop phosphatase inhibitor tablets (Thermofisher Scientific) and cOmplete™protease EDTA-free inhibitor (Millipore Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... 1% v/v Triton X-100) supplemented with EDTA-free Pierce Protease and Phosphatase Inhibitor Mini Tables (ThermoFisher Scientific) using a FastPrep-24 bead mill (MP Biomedicals ...
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bioRxiv - Microbiology 2024Quote: ... low-adhesion plates in 100 µL of N2B27 differentiation medium (1:1 [v/v] of DMEM/F-12 [GIBCO] and neurobasal medium [GIBCO] supplemented to contain 10% Knockout serum replacement [Invitrogen] ...
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bioRxiv - Neuroscience 2024Quote: ... The PBSDT was then removed and replaced with 10% v/v CAS-Block™ (008120, Invitrogen, Scoresby, VIC, Australia). The blocking agent was then removed and replaced with primary antibodies (Supp Table 1 ...
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bioRxiv - Bioengineering 2024Quote: ... EBs and BOs were permeabilized with permeabilization buffer (0.3% Triton X-100 (neoFroxx, GmbH, Cat no. 8500) (v/v) in PBS (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... purified TOG5-GFP construct was desalted into EM Buffer (BRB80 plus 5 mM BME and 0.05% Tween-20 (v/v)) using a 0.5 mL 7K MWCO Zeba Spin Desalting Column (Thermo Scientific). After desalting ...
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bioRxiv - Microbiology 2024Quote: ... 80-90% confluent cells were split by washing with sterile PBS and incubation with 0.25% (v/v) trypsin-EDTA (25200056, Gibco) for 3 min ...
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bioRxiv - Plant Biology 2024Quote: ... Eluate phosphopeptides were then acidified with formic acid to a concentration of 0.5% (v/v) and dried with speed vacuum (ThermoFisher). Dried phosphopeptides were then re-suspended in 800 µL ice-cold immunoprecipitation (IP ...
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bioRxiv - Neuroscience 2024Quote: ... The vector was dialyzed against phosphate-buffered saline (PBS) containing 0.001% (v/v) Pluronic-F68 (#24040032; Thermo Fisher Scientific) using Vivaspin Turbo 15 100,000 MWCO (#VS15T41 ...
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bioRxiv - Molecular Biology 2024Quote: H7 hESCs (WiCell, WAe007-A) were cultured on plates pre-coated with 1% (v/v) Geltrex (Thermo Fisher, A1413302) with complete Essential 8 medium (Thermo Fisher ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% (v/v) heat-inactivated fetal calf serum (Fetal Calf Serum; 10500-064, Gibco, Invitrogen, Carlsbad, CA), 2 mM L-glutamine (17-605E ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 10% (v/v) heat-inactivated fetal calf serum (Fetal Calf Serum; 10500-064, Gibco, Invitrogen, Carlsbad, CA), 2 mM L-glutamine (17-605E ...
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bioRxiv - Bioengineering 2024Quote: ... and buffer was exchanged to phosphate buffered saline (PBS) containing 0.001% v/v Pluronic F68 (Gibco, Grand Island, NY) using 7-kDa molecular weight cutoff Zeba desalting columns (Thermo Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... and 50% (v/v) glycerol] using Slide-A-lyzer Mini dialysis device (10 K MWCO, 0.5 mL, ThermoFisher Scientific). Fractions and final Rrn3 preparations were quality controlled by SDS-PAGE and Western analysis using Coomassie staining and monoclonal anti-His-tag antibody ( 6X His Tag Antibody Dylight™ 800 Conjugated ...
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bioRxiv - Genomics 2024Quote: ... Cells were then briefly washed with DPBS and permeabilized with 0.5% (v/v) triton-X 100 (Thermo Fisher; 28314) in PBS for 10 min with rocking ...
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bioRxiv - Biochemistry 2024Quote: ... 100 mM to 1 M NaCl) and purity was ensured by 2% agarose gel supplemented with 0.1% (v/v) Sybr Safe dye (Invitrogen) for visualization.
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bioRxiv - Biophysics 2024Quote: ... HEK293T cells grown in 100- mm dishes with Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% (v/v) fetal bovine serum (Invitrogen), penicillin (100 U/ml) ...
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bioRxiv - Bioengineering 2024Quote: ... A suspension of 0.05% v/v 1-µm fluorescent polystyrene spheres (FluoSpheres, excitation/emission 540/560 nm, Molecular Probes) in aqueous 0.85% w/v methyl cellulose was infused into both device inlets using syringe pumps (Pump33 DDS and Pump11 Elite ...
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bioRxiv - Systems Biology 2024Quote: ... IL-1 and TNF treatments were prepared with Alexa Fluor 647–conjugated BSA (0.0025% v/v of a 5 mg/mL stock; Invitrogen) to visualize the cytokine containing stream in the device.
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bioRxiv - Bioengineering 2024Quote: ... HeLa and A549 were cultured at 37 °C and 5 % CO2 in Dulbecco’s Modified Eagle’s Medium (4500 mg/L glucose) supplemented with 10 % (v/v) heat-inactivated fetal bovine serum (hi-FCS, GIBCO) and 1 % (v/v ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with 5% v/v fetal bovine serum (FBS) and were transiently transfected with plasmids using lipofectamine 2000 (Invitrogen) as described ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% v/v heat-inactivated fetal bovine serum (S1810, Bio-West, Nuaillé, France) and 1% v/v penicillin–streptomycin (15,140-122, Gibco). Upon reaching 90% confluence ...
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bioRxiv - Biochemistry 2024Quote: ... CH3CN and 0.3% (v/v) formic acid] on a 2.1 mm-by-50 mm C18 separation column (Hypersil Gold, ThermoFisher Scientific) for 5 min ...