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Citations for Thermo Fisher :
301 - 350 of 409 citations for Mycoplasma Pneumoniae Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: All cells are routinely tested for mycoplasma by PCR kit (ATCC, Manassas, VA) and grown in complete media containing DMEM (Gibco, Carlsbad, CA), 10% FBS (Peak Serum ...
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bioRxiv - Immunology 2021Quote: ... for 2-18 hours at 4°C and stained with cocktails of fluorescently-labeled antibodies against intracellular antigens diluted in Permeabilization Buffer (Invitrogen) for 20 minutes at room temperature followed by 20 minutes at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... anti-mouse surface antigen 1 (SAG1) mab DG52 1:500 46 and then with goat anti-rat-Alexa546 1:300 (Invitrogen), goat anti-rat-Alexa375 1:300 (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: All antigens were biotinylated using an NHS-Sulfo-LC-LC kit according to the manufacturer’s instruction (Thermo Fisher, MA, USA) if required by the assay and excessive biotin was removed by size exclusion chromatography using Zeba-Spin desalting columns (7kDa cutoff ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Set overnight and stained with antibodies against intracellular antigens (1 µg/ml) in Permeabilization buffer (ThermoFisher). For biotinylated antibodies ...
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bioRxiv - Immunology 2021Quote: ... Induced yeast libraries were stained for antigen binding (RBD-Fc APC tetramers) and scFv expression (chicken anti-c-myc IgY; Invitrogen). Following two washes in PBSF (1x PBS ...
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bioRxiv - Bioengineering 2019Quote: Paraffin-embedded aorta sections mounted slides were subjected to heat-induced antigen epitope retrieval with citrate buffer (Thermo Scientific, MA). The slides were incubated overnight at 4°C with primary antibodies for anti -MMP-9 (Rabbit anti-Mouse ...
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bioRxiv - Microbiology 2021Quote: ... fixed parasites were incubated with 5µg/mL Blood Group Antigen H (O) Type 1 monoclonal antibody (Invitrogen 13-9810-82), washed three times with 1x PBS and incubated with 1:100 α-mouse secondary antibody conjugated to Alexa 488 (Invitrogen ...
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bioRxiv - Bioengineering 2022Quote: ... with 50 µL per injection site of vaccine formulations containing 5 µg of SARS-CoV-2 antigen (RBD-SD1, S-2P, S-PCNA1, or 6RBD-np) gently mixed 1:1 (v/v) with AddaVax (Invitrogen) adjuvant ...
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bioRxiv - Immunology 2020Quote: ... Phage display selections were carried out by alternating between capture of the antigen on neutravidin coated wells on Maxisorp plates (Nunc) and streptavidin magnetic beads (Invitrogen ...
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bioRxiv - Immunology 2022Quote: The uptake and processing of CSF-derived protein by antigen-presenting cells outside the CNS was evaluated by injecting 3 μl of DQ-OVA (2 mg/ml, Invitrogen) into the CSF of chronically infected C57BL/6 mice by i.c.m ...
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bioRxiv - Immunology 2020Quote: ... surface antigen-labeled cells were fixed and permeabilized with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific) and incubated with the fluorochrome-labeled antibodies listed in Table S3 ...
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bioRxiv - Microbiology 2021Quote: ... Mice received 20 μg protein antigen (vaccinated) or PBS (mock-vaccinated) mixed 1:1 with Imject Alum adjuvant (Thermofisher Scientific) in a total volume of 100 μl ...
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bioRxiv - Immunology 2020Quote: Serum antibodies were purified from 25mL of remaining serum of final bleeds using antigen conjugated to NHS-activated agarose resin (ThermoFisher). Columns were washed three times with 1X PBS buffer ...
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bioRxiv - Immunology 2020Quote: To address neutrophils activation and phagocytic capacities we used pHrodo-labeled BioParticles and coated with Staphylococcus aureus (S. aureus) or Zymosan antigens (Invitrogen). pH-sensitive pHrodo dyes are useful for visualizing acidification process during phago-lysosome formation (29) ...
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bioRxiv - Immunology 2020Quote: ... Each mouse received 50 μg of antigen in 150 μL of PBS (NP40‑FICOLL) or PBS/Alum (77161, Thermo Fisher) adjuvant (2:1 ratio ...
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bioRxiv - Immunology 2020Quote: ... braziliensis (MHOM/BR/1975/M2903) soluble antigen (2 μg/well) were adsorbed in microtiter plate (Nunc-immuno Plate, Roskilde, Denmark). Then ...
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bioRxiv - Immunology 2021Quote: ... Thymocytes were stained for extracellular antigens before EdU detection with click-iT Plus EdU Alexa Fluor 488 Flow Cytometry Assay Kit (Invitrogen), following the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker and then incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... slides were placed in metal-free water for 5 minutes on an orbital shaker after which they were incubated in pH 9 IHC Antigen Retrieval Solution (Invitrogen) at 96°C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... Primary cells were immortalized by transfection of pRSVAg1 plasmid expressing Simian Vacuolating Virus 40 large T antigen (SV40T) with lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... mice were injected sub-cutaneously with 10 μg of fluorescent antigen in 100 μL mixed 1:1 with Imject Alum Adjuvant (ThermoFisher) in the upper and lower flank to target the brachial ...
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bioRxiv - Immunology 2022Quote: ... for 2-18 hours at 4°C and stained with cocktails of fluorescently labeled antibodies against intracellular antigens diluted in Permeabilization Buffer (Invitrogen) for 30 minutes at 4°C.
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bioRxiv - Developmental Biology 2022Quote: ... Overnight incubations (4°C) of the sections were carried out with mouse monoclonal anti- proliferating cell nuclear antigen (PCNA) (1:200, Invitrogen). Control sections were incubated with a mouse IgG Isotype Control (1:200 ...
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bioRxiv - Immunology 2023Quote: ... 1-2 x 106 antigen-experienced 5c.c7 TCR-transgenic T-cells were incubated with 2 µM Fura-2-AM (Life technologies) in T-cell growth medium for 15-20 minutes at room temperature and subsequently washed with warm (room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... de-paraffinized and re-hydrated serial sections were subjected to antigen retrieval using 1x PT module buffer 1 (Thermo Scientific) for 36 min at 93°C using a KOS microwave Histostation (Milestone) ...
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bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... Antigen retrieval was performed by heating the slides in 10 mM sodium citrate buffer pH 6.0 (Thermo Fisher, S279-3) for 4 min in a microwave and left to cool down for 10 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... for 2-18 hours at 4°C and stained with cocktails of fluorescently-labeled antibodies against intracellular antigens diluted in Permeabilization Buffer (Invitrogen) for 30 minutes at 4°C ...
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bioRxiv - Bioengineering 2023Quote: Immunostaining of AADC was performed as follows: (1) incubate the brain sections in 1X antigen retrieval solution (catalog number: 00-4955-58; Invitrogen) overnight in a 60°C water bath ...
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bioRxiv - Neuroscience 2023Quote: ... by RT-PCR (see methods below) and (ii) by measuring the expression of pluripotency surface antigen (SSEA4 1:20, Invitrogen, 12-8843-42 ...
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bioRxiv - Microbiology 2023Quote: Human embryonic kidney cells expressing SV40 large T-antigen (HEK293T, ATCC®) were cultured in Dulbecco’s modified eagle’s medium (Gibco®) supplemented with 100 units/ml penicillin and 100 µg/ml streptomycin (pen/strep ...
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bioRxiv - Immunology 2024Quote: ... antigen proteins of the target were biotinylated using the EZ-link™Sulfo-NHS-LC-LC-Biotin kit (Thermo Fisher), then coupled to the fluorescent neutravidin beads (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... were conducted to remove trace PFA followed by 5 min antigen retrieval with SDS (1% Sodium Dodecyl Sulfate; Fisher Scientific) in PBS ...
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bioRxiv - Cancer Biology 2024Quote: The human non-small cell lung cancer cell line H1299 and the human embryonic kidney cell line containing the SV40 T-antigen HEK293T were maintained in Dulbeccós Modified Eaglés Medium (DMEM, 31600091, Thermo Fisher Scientific) supplemented with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Bioengineering 2019Quote: ... Jurkat and Human B-lymphoid cell lines were initially tested for mycoplasma contamination and then cultured in RPMI-1640 medium (Life Technologies, Zug, Switzerland) supplemented with 10% (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... All the cell lines were tested for mycoplasma contamination and were expanded in DMEM supplemented with 10% (v/v) foetal bovine serum (FBS; GIBCO, Life Technologies, Carlsbad, USA), 0.1 mg/mL streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... GroEL was detected by monoclonal mouse antibody that detects antigens present on all three GroEL paralogs (MA3-023, Invitrogen, ThermoFisher Scientific). Primary antibody incubation was allowed to proceed overnight at 4° C with rocking ...
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bioRxiv - Microbiology 2020Quote: ... GroEL was detected by monoclonal mouse antibody that detects antigens present on all three GroEL paralogs (MA3-023, Invitrogen, ThermoFisher Scientific). Primary antibody incubation was allowed to proceed overnight at 4° C with rocking ...
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bioRxiv - Biophysics 2019Quote: ... K562 feeder cells expressing the cognate CAR antigen ROR1 (K562-ROR1) were used to stimulate CAR T cells and maintained in 1640 RPMI media (Life technologies) supplemented with 10 % FCS (Merck) ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated with an antibody that recognizes the Cut antigen (mouse 1:50, DSHB) followed by a fluorescent-conjugated secondary antibody (Alexa Fluor, Molecular Probes). For quantitative purposes ...
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bioRxiv - Cancer Biology 2020Quote: ... The phage library was incubated with biotinylated CD40-Fc fusion protein or Her2 recombinant protein (Acro biosystems) for 2 hours at RT and the phage-antigen complex was captured by Dynabeads M280 (Life technologies). The bound phages were eluted by Glycin-HCl (pH 2.2 ...
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bioRxiv - Bioengineering 2020Quote: ... and subjected to heat-induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, Fisher Scientific International, Inc., Pittsburgh, MA) at >80°C ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity to the antigen was visualized using Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (Molecular Probes, Eugene, OR). Nuclear staining was performed with Hoechst 33258 (Polysciences ...
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bioRxiv - Immunology 2022Quote: ... B/Colorado/06/2017) were chosen as a historical antigen and were biotinylated using an EZ-Link Micro NHS-PEG4 Biotinylation Kit (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... with the following modifications (1) antigen retrieval was performed using low pH IHC Ag Retrieval Solution (Thermo Fisher, 00-4955-58) and subjected to heat using a steamer for 40 minutes ...
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bioRxiv - Immunology 2022Quote: ... the cells were first stained for live/dead discrimination followed by surface antigen staining and subsequently fixed using Foxp3 fixation/permeabilization buffer (Thermo Fisher) for 1 hour at 4°C ...
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bioRxiv - Immunology 2019Quote: ... mice were inoculated s.c with overnight ES antigens emulsified with an equal volume of Alum-an aluminum salt adjuvant (88) obtained from Thermo Scientific to achieve a vaccine dose of 100 μg ES in 100 μl Alum as a positive control ...
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bioRxiv - Microbiology 2020Quote: ... LAM antigen was diluted to the desired concentration in control human serum in high-recovery glass vials (Thermo Scientific, Waltham, MA) C5000-995 and incubated overnight (18−24 hrs ...
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bioRxiv - Immunology 2021Quote: ... Cytokine concentrations in the day 6 supernatants of antigen-stimulated cultures were assessed by Luminex bead-based assay (Thermo Fisher Scientific) according to the manufacturer’s instructions ...