Labshake search
Citations for Thermo Fisher :
1 - 50 of 409 citations for Mycoplasma Pneumoniae Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... pneumoniae (Invitrogen) and rabbit anti-P ...
-
bioRxiv - Microbiology 2023Quote: ... pneumoniae (Thermo Fisher Scientific), ZO-1 (Invitrogen) ...
-
bioRxiv - Microbiology 2022Quote: ... pneumoniae was added to 100% active human (Invitrogen, Waltham, MA) or C57B/L6 murine serum (Invitrogen) ...
-
bioRxiv - Microbiology 2019Quote: ... pneumoniae isolates were stained with BacLight 488 (Thermo Scientific, USA) with slight changes to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2022Quote: ... pneumoniae strain D39 diluted in phosphate-buffered saline (PBS; Thermo Scientific) into the lateral tail vein [15 ...
-
bioRxiv - Microbiology 2023Quote: ... The following antibodies were used for immunostaining: anti-Klebsiella pneumoniae (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... pneumoniae and PMN depletion was confirmed using anti-Gr-1 Rb6-antibodies (Invitrogen).
-
bioRxiv - Microbiology 2020Quote: ... pneumoniae and followed with Alexa Fluor 488 goat anti rabbit antibody (Thermo Fisher Scientific). SH-SY5Y cells were stained with Phalloidin 594 (Thermo Fisher Scientific) ...
-
bioRxiv - Immunology 2019Quote: ... pneumoniae for 6 hours in 1% FCS phenol free alpha MEM (base media, Life Technologies). Cells were washed three times in PBS and gently lifted from the plate using a cell scraper in 300μl of base media supplemented with 1mM EDTA ...
-
bioRxiv - Neuroscience 2023Quote: ... Antigen retrieval was performed with Low pH IHC Antigen Retrieval Solution (Invitrogen) in a pressure cooker set to 100°C for 10 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells are tested for mycoplasma using the MycoFluor Mycoplasma Detection Kit (ThermoFisher, Waltham, MA, USA).
-
bioRxiv - Bioengineering 2024Quote: ... and Mycoplasma testing within 6 months with the MycoStrip Mycoplasma Detection Kit (Invitrogen, Waltham, MA). Cell lines were maintained in culture at 37°C in 5% CO2.
-
bioRxiv - Bioengineering 2024Quote: ... and Mycoplasma testing within 6 months using the MycoStrip Mycoplasma Detection Kit (Invitrogen, Waltham, MA). Cell lines were maintained in culture at 37°C in 5% CO2.
-
bioRxiv - Microbiology 2022Quote: ... pneumoniae K5962 and ϕKp24 were clipped and loaded into a Titan Krios (Thermo Fisher Scientific (TFS)) equipped with a K3 BioQuantum direct electron detector and an energy filter (Gatan ...
-
bioRxiv - Microbiology 2023Quote: ... pneumoniae KP35 were amplified by PCR using the Platinum SuperFi II High Fidelity DNA Polymerase (Invitrogen), and then joined by Gibson assembly (Fig ...
-
bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval was performed using IHC Antigen Retrieval Solution (ThermoFisher, 00-4955-58) in a steamer for 40Dminutes ...
-
bioRxiv - Cell Biology 2020Quote: ... Mycoplasma contamination was assessed with a MycoFluor™ Mycoplasma Detection Kit (Catalog M7006, Thermo Fisher Scientific) and cells used for experiments were free of mycoplasma contamination.
-
bioRxiv - Biochemistry 2022Quote: ... All iPSC lines were also screened for mycoplasma contamination using a mycoplasma detection kit (ThermoFisher, 4460623). Once the test came back negative ...
-
bioRxiv - Immunology 2021Quote: ... Mycoplasma analysis was performed with Mycoplasma species 500 PCR kit at GeneAmp PCR System 2700 (Applied Biosystems). Quality control tests of the vaccine including levels of chemistry analysis (Na ...
-
bioRxiv - Microbiology 2021Quote: ... pneumoniae (one colony per species per plate) were further sub-cultured on Sheep Blood Agar plates (ThermoFisher) and species identity confirmed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS ...
-
bioRxiv - Microbiology 2022Quote: ... pneumoniae serotype 2 strain D39 [17] was plated on brain heart infusion (BHI, Thermo Scientific, MA, USA) agar plates supplemented with 3% v/v defibrinated horse blood (Thermo Scientific ...
-
bioRxiv - Immunology 2020Quote: ... pneumoniae isolates were stored at −80°C in Luria-Bertani broth (LB; Thermo Fisher Scientific, Waltham, MA) supplemented with 15% glycerol.
-
bioRxiv - Microbiology 2024Quote: ... pneumoniae strains to a range of antibiotics was determined using broth microdilution (Trek Sensititre®, ThermoFisher Scientific) as per manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... Testing for mycoplasma contamination was performed using the MicroFluor Mycoplasma Detection kit (catalog #M7006; ThermoFisher Scientific, Waltham, MA) at the time of the last passage and no contamination was detected.
-
bioRxiv - Cell Biology 2023Quote: ... All cell lines were certified free of Mycoplasma either internally using the MycoSEQ Mycoplasma detection kit (Thermo Fisher) or by IDEXX BioAnalytics using STAT-Myco testing ...
-
bioRxiv - Systems Biology 2024Quote: ... Cells were all confirmed to be mycoplasma negative using a PCR mycoplasma detection kit (Thermo Fisher Chemicals, J66117) following manufacturers instructions.
-
bioRxiv - Immunology 2022Quote: ... native control peptides and control antigens using a custom-designed antigen microarray (ThermoFisher Scientific, Immunodiagnostics)9 ...
-
bioRxiv - Physiology 2022Quote: ... Targeted antigen retrieval was performed by boiling sections in 1x IHC antigen retrieval solution (Invitrogen) for 15-20 mins ...
-
bioRxiv - Microbiology 2020Quote: ... IHC for Spn was performed using rabbit anti-Streptococcus pneumoniae polyclonal antibody (Thermo Fisher scientific cat. # PA-7259) followed by biotin-labeled goat anti-rabbit IgG antibody (Thermo Fisher Scientific Cat.# ...
-
bioRxiv - Microbiology 2020Quote: ... pneumoniae transformants were selected by plating inside Columbia agar supplemented with 3% of defibrinated sheep blood (Thermo Scientific), followed by antibiotic selection ...
-
bioRxiv - Immunology 2020Quote: ... pneumoniae was then spun down at 5000G for 5 minutes and resuspended in 1mL 1XPBS (Gibco, Gaithersburg, MD). Sub-cultured K ...
-
bioRxiv - Immunology 2023Quote: ... or control antigen PE (Thermo Fisher) were mixed with adjuvants per manufacturer’s protocols and added to cells immediately after plating ...
-
bioRxiv - Immunology 2023Quote: ... Monomeric antigens were biotinylated (ThermoFisher; A39257) at an optimized ratio which resulted in <1 biotin per molecule ...
-
bioRxiv - Neuroscience 2022Quote: All human pluripotent stem cells were maintained below passage 30 and confirmed negative for mycoplasma using the MycoFluor Mycoplasma Detection Kit (ThermoFisher). iPSCs were regularly tested for pluripotency using OCT4 ...
-
bioRxiv - Microbiology 2021Quote: ... pneumoniae strains were grown overnight at 37°C to stationary phase in LB-Lennox broth (Fisher Scientific, BP1427-2) with constant agitation ...
-
bioRxiv - Immunology 2020Quote: ELISA of SARS-CoV-2 antigens was performed by coating antigens on MaxiSorp 96-well plates (ThermoFisher) at 2 µg/mL in 1X PBS [pH 7.4] overnight at 4 °C ...
-
bioRxiv - Cancer Biology 2023Quote: ... antigen retrieval techniques were applied human tissue sections using IHC Antigen Retrieval Solution (00-4956-58, Invitrogen). These tissues were stained with Abs against human CD4 pre-labeled with Alexa Fluor 488 using an Ab labeling kit (Molecular Probes ...
-
bioRxiv - Cancer Biology 2021Quote: OCI-AML3 and OCI-AML2 cell lines were obtained from the DSMZ and monthly tested for mycoplasma infections using the MycoFluor™ Mycoplasma Detection Kit (Invitrogen). AML cell lines and primary cells were cultured in RPMI at 37°C in a humidified incubator with 5 % CO2 ...
-
bioRxiv - Genomics 2021Quote: ... mycoplasma tested) and HG002 cells (Coriell Institute, Camden, NJ; mycoplasma tested) were maintained in RPMI-1640 with L-glutamine (Gibco 11875093) supplemented with 15% Fetal Bovine Serum (VWR 89510-186 ...
-
bioRxiv - Cancer Biology 2024Quote: ... brain slices underwent an additional antigen retrieval step in 1X IHC Antigen Retrieval Solution (Invitrogen, 00-4956-58) for 15 minutes at 95°C prior to blocking.
-
bioRxiv - Immunology 2019Quote: Mucosal curettage samples and epithelial cell cultures (incubated with or without S. pneumoniae for 3 hours) were collected in RNALater (ThermoFisher) and stored at −80°C ...
-
bioRxiv - Microbiology 2024Quote: Heat-killed Streptococcus pneumoniae (HK-Spn) was prepared by growing wild-type D39 in Todd-Hewitt Broth (Thermo Scientific, CM0189B) with 2% Yeast Extract (Sigma ...
-
bioRxiv - Microbiology 2019Quote: ... All cells were maintained in a humidified incubator at 37°C and 5% CO2 and were found free of mycoplasma contamination on repeated testing with the MycoFluor Mycoplasma Detection Kit (Life Technologies, UK). MDCK II cells were treated as previously19 with the following modifications ...
-
bioRxiv - Cell Biology 2022Quote: ... 5% CO2 were tested for mycoplasma using PlasmoTest (Invitrogen). Before use the Serum Plus II Medium Supplement was heat inactivated by incubation at 56°C for 30 minutes.
-
bioRxiv - Cancer Biology 2023Quote: ... after which heat-induced antigen retrieval was performed in 1x low pH antigen retrieval solution (pH6, Thermo Fisher Scientific). Subsequently ...
-
bioRxiv - Neuroscience 2023Quote: ... A mild antigen retrieval step was introduced by immersing the slides into boiled antigen retrieval solution (00-4955-58, Invitrogen) for 10 min ...
-
bioRxiv - Immunology 2020Quote: ... antigens were directly coated to Nunc-absorb (ThermoFisher) plates overnight at 4°C or captured using streptavidin ...
-
bioRxiv - Immunology 2023Quote: ... Antigen printed pads were blocked with SuperBlock (ThermoFisher) for 1 hour before probed with serum samples (diluted at 1:100 in SuperBlock) ...
-
bioRxiv - Physiology 2024Quote: ... heated in antigen retrieval solution (Thermo Fisher Scientific) at 100 °C for 20 min ...
-
bioRxiv - Molecular Biology 2020Quote: Mouse NIH3T3 fibroblasts (mycoplasma-free) were maintained in DMEM (Gibco) with 10% fetal calf serum (FCS ...