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Citations for Thermo Fisher :
251 - 300 of 409 citations for Mycoplasma Pneumoniae Antigen since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... rabbit or sheep antigens were raised in donkey and conjugated to Alexa 488 or Alexa 594 (Molecular Probes, ThermoFisher ...
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bioRxiv - Bioengineering 2021Quote: ... and subjected to heat- induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, ThermoFisher, Waltham, MA) at >80°C ...
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bioRxiv - Microbiology 2022Quote: Purified F antigens were biotinylated using an EZ-link Sulfo-NHS -LC-Biotinylation kit (Thermo Fisher, cat#A39257) with a 1:1.3 molar ratio of biotin to F ...
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bioRxiv - Microbiology 2019Quote: ... SV40 T-antigens were detected using (pAb)108 and species-specific AlexaFluor 488 (Life Technologies, Thermo Fisher Scientific) antibodies ...
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bioRxiv - Molecular Biology 2021Quote: ... The antiserum was affinity-purified using the peptide antigen coupled to UltraLink™ Iodoacetyl gel (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... after dewaxing and antigen retrieval non-specific antibody binding was minimised by incubating sections in CAS Block (Invitrogen). Primary antibodies were diluted in Dako antibody diluent (S0809 ...
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bioRxiv - Plant Biology 2022Quote: ... Chemiluminescence detection of antigen-antibody complexes was carried out with SuperSignal West Femto Western substrate (Thermo Fisher Scientific). As a loading control ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody/antigen mixture was incubated with Dynabeads M-280 sheep anti-rabbit or mouse IgG (Thermo Fisher Scientific) for 6h at 4°C in the following day ...
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bioRxiv - Biophysics 2023Quote: ... 200 μL of the virus dilution containing 1µg-3µg p24 antigen was transferred to a poly-D-lysine coated glass coverslip (#1 chambered coverglass, Nunc™ Lab-Tek™ ...
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bioRxiv - Neuroscience 2023Quote: ... For antigen retrieval cells were incubated for ten minutes with 1 ug/ml proteinase K (Thermo Fisher Scientific) at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and coronavirus antigens were expressed in Expi293F cells by transient transfection in FreeStyle F17 expression media (Thermo Fisher) supplemented to a final concentration of 0.1% Pluronic Acid F-68 and 20% 4 mM L-glutamine using Expifectamine transfection reagent (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2023Quote: ... the antigens were captured for 15 mins at 4 °C with 50 µl Streptavidin Magnetic beads (Thermo Fisher) which were previously incubated with superblock (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... Target antigens were finally detected using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Waltham, MA). Images were scanned and analyzed using ImageJ software ...
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bioRxiv - Biochemistry 2020Quote: ... The P2 baculovirus produced in Sf9 cells was added to HEK293 GnTI- cells (mycoplasma test negative, ATCC #CRL-3022) and grown in suspension in FreeStyle medium (GIBCO-Life Technologies) supplemented with 2% FBS at 37°C and 8% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... approximately 450,000 mycoplasma-free cells were seeded into two wells of a 6-well plate in DMEM (11995-065; Thermo Fisher Scientific) containing 10% FBS (PS-FB3 ...
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bioRxiv - Microbiology 2022Quote: ... HTB-14) were obtained from ATCC as mycoplasma-free stocks and grown in Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific) supplemented with 10% (v / v ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were routinely checked for mycoplasma and determined to be free of contamination through the absence of extranuclear Hoechst 33342 (Life Technologies) staining.
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bioRxiv - Genomics 2021Quote: ... validated by microsatellite typing and mycoplasma tested) were maintained in DMEM (high glucose, with GlutaMAX, with phenol red, without sodium pyruvate; Gibco 10566016) supplemented with 10% Fetal Bovine Serum (VWR 89510-186 ...
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bioRxiv - Biochemistry 2022Quote: The resulting construct (OR51E2-miniGs399) was transfected into 1 L of inducible Expi293F-TetR cells (unauthenticated and untested for mycoplasma contamination, Thermo Fisher) using the ExpiFectamine 293 Transfection Kit (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were placed in 250 ml freshly made antigen retrieval solution (0.1 M sodium citrate buffer pH 6.0, Fisher Scientific) and pressure cooked (A ...
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bioRxiv - Immunology 2022Quote: ... The antigen binding fragment (Fab) portion of LY-CoV1404 was generated by proteolytic digestion using immobilized papain (ThermoFisher Scientific), followed by removal of un-cleaved protein using standard chromatography techniques ...
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bioRxiv - Systems Biology 2019Quote: Recombinant protein antigens were first labeled with biotin using EZ-Link™ NHS-LC-Biotin reagent (Thermo Fisher, #21366) and then hybridized with the PETAL array ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity to the antigen was visualized using Alexa Fluor 488-or Alexa Fluor 594-conjugated secondary antibody (Molecular Probes). Nuclear staining was performed with Hoechst 33258 (Polysciences) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were washed in PBS and incubated for 1-h at RT with 1 mL anti-Early Endosomal Antigen 1 (EEA1) monoclonal antibody (F. 43.1; Invitrogen) diluted 1:200 in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit or sheep antigens were raised in donkey and conjugated to Alexa 488 or Alexa 594 (Molecular Probes, ThermoFisher, RRIDs ...
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bioRxiv - Immunology 2020Quote: ... Anti-mouse monoclonal antibodies targeting cell surface antigens included anti-GL7 (FITC, PE, eFluor660; clone GL 7; Thermo Fisher), anti-B220 (PE-Cy7 ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigens were coupled to magnetic Luminex beads (Luminex Corp) by carbodiimide-NHS ester-coupling (Thermo Fisher). Coupled beads were incubated for 2 hours at 37°C with serum samples (1:10 dilution ...
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bioRxiv - Immunology 2022Quote: Vaccine 5: The above mentioned hCMP-mRBD antigen when expressed from Stable CHO (Thermo Fisher Scientific, Cat no. R75807) cell lines was named as hCMP-mRBD-CHO ...
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bioRxiv - Cancer Biology 2020Quote: ... The beads with antigens captured were loaded onto a SDS-PAGE gel (4-20% gradient polyacrylamide) (Thermo Fisher Scientific) in duplicates ...
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bioRxiv - Immunology 2020Quote: ... Antigen-specific IgG was purified from pooled serum with UF6b-conjugated Dynabeads™ M-270 Epoxy (Thermo Fisher Scientific) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... The antigen peptide with N-terminus Cysteine (NH2-CGGGGAAPGPPRHG-COOH) was coupled with keyhole limpet hemocyanin (Thermo fisher 77600). The antigen was injected into rabbit and antisera were collected after primary and several booster injections ...
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bioRxiv - Cancer Biology 2021Quote: ... The antigen-antibody complexes on the membrane were detected using labeled secondary antibodies and enhanced chemiluminescence reagents (Thermo Scientific). The antibodies and their dilutions were as follows ...
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bioRxiv - Immunology 2020Quote: ... total IgG against these antigens were detected using HRP-conjugated respective anti-mouse antibody (Sigma-Aldrich, eBioscience and Invitrogen) and the reaction was detected using TMB substrate (BD biosciences) ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μL of capture Ab (~4 μg/ml) or SARS-CoV-2 recombinant antigen (~0.2 mg/ml) in 1x PBS (Gibco) was loaded in each channel ...
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bioRxiv - Biochemistry 2022Quote: ... and LacI antibodies were affinity-purified from serum using the appropriate antigen coupled to AminoLink Coupling Resin (Thermo Scientific) according to manufacturer’s protocol.
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bioRxiv - Developmental Biology 2022Quote: ... were used to detect antigens and DNA was stained with DAPI in the mounting medium (ThermoFisher Scientific, Cat# P36941).
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bioRxiv - Physiology 2022Quote: ... Non-specific antigens were blocked for 60 min using a blocking buffer (Sea Block Blocking Buffer; ThermoFisher Scientific, USA). Then ...
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bioRxiv - Microbiology 2022Quote: All antigens and FcγRs were equilibrated in 1X PBS using Zeba-Spin desalting and size exclusion chromatography columns (ThermoFisher) prior to bead coupling ...
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bioRxiv - Cancer Biology 2023Quote: ... The enzymatic antigen retrieval was performed by incubating the sections for 2 min with TrypLE™ Express Enzyme (ThermoFisher) at 37 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The antigen-antibody complex was detected by SuperSignal™ West Dura Extended Duration Chemiluminescent Substrate (Cat#34075, Thermo Fisher).
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bioRxiv - Molecular Biology 2019Quote: ... cells (in-house stock regularly tested to be virus- and mycoplasma-free) were grown at 25°C in S2 cell media supplemented with 10% fetal bovine serum (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2020Quote: ... Cells used during this study were less than passage 30 and confirmed to be free of mycoplasma contamination by Hoescht staining (Thermofisher Cat#62249) as per manufacturer’s protocol.
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bioRxiv - Biochemistry 2020Quote: ... The P2 baculovirus produced in Sf9 cells was added to HEK293 GnTI- cells (mycoplasma test negative, ATCC #CRL-3022) and grown in suspension in FreeStyle medium (GIBCO-Life Technologies) supplemented with 2% FBS at 37°C and 8% CO2 ...
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bioRxiv - Microbiology 2022Quote: ... were obtained from ATCC (CRL-1586) (Manassas, VA, USA) as mycoplasma-free stocks and were grown in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific (TFS), Merelbeke ...
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bioRxiv - Microbiology 2022Quote: Pelleting of Mycoplasma cells from broth cultures was performed using 50 ml tubes with a high-speed Fiberlite™ centrifuge rotor (Thermo Scientific). For small broth cultures ...
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bioRxiv - Cancer Biology 2020Quote: ... These cell lines, which tested negative for mycoplasma at thawing, were maintained in T2 medium (RPMI, 10% FBS (HyClone, Thermo Scientific, SH3010903), 1mM sodium pyruvate ...
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bioRxiv - Biochemistry 2021Quote: HEK293T (cell line authenticated and tested without mycoplasma contamination) nuclear lysate is extracted using NE-PER™ Nuclear extraction kit from Thermo Scientific™ ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting NK1R-miniGs/q70 fusion construct was transfected into adherent Expi293F™ Inducible Human Embryonic Kidney Cells (unauthenticated and untested for mycoplasma contamination, Life Technologies) using Lipofectamine 2000Ω and cells were maintained in DMEM (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... The subsequent NK1R-miniGs399fusion construct was transiently transfected into 200-mLs of Expi293F™ Inducible Human Embryonic Kidney Cells (unauthenticated and untested for mycoplasma contamination, Life Technologies) using the Expifectamine Transfection Kit (Life Technologies) ...