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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... with 5% β-mercaptoethanol at 50°C for 10mins and loaded onto a 4-20% Tris-glycine SDS-PAGE gel (Invitrogen). The proteins were transferred to a nitrocellulose membrane with an iBlot (ThermoFischer) ...
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bioRxiv - Cancer Biology 2023Quote: ... 5-20 μg of total protein was separated by NuPAGE 4-12% Bis-Tris Mini Protein Gels (NP0336BOX; Thermo Fisher Scientific) and transferred to Immobilon-P PVDF Membrane (IPVH00010 ...
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bioRxiv - Microbiology 2024Quote: ... 20% volume of 1M 2-morpholin-4-ylethanesulfonic acid (MES) pH 5 and Immobilized Protein G resin (Thermo Scientific Prod#20397) were added to supernatant and sample was incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were heated at 95°C for 5 min and proteins resolved on 4–20% acrylamide Tris-glycine gradient gels (Life Technologies). Proteins were then transferred to polyvinylidene difluoride membranes (EMD Millipore ...
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bioRxiv - Biophysics 2024Quote: ... 1 µL of the completed PCR reactions was then combined with 4 µL of water and 5 µL of print buffer (2x: 20 mg/mL UltraPure BSA (Thermo Fisher), 24 mg/mL trehalose dihydrate ...
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bioRxiv - Microbiology 2020Quote: ... 20 μl of 5 mg/ml glycogen (Ambion), and 1 ml of cold isopropanol ...
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bioRxiv - Bioengineering 2023Quote: ... + 20 uM fluorescein-5-maleimide cytoplasm stain (ThermoFisher) + 0.1% (v/v ...
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bioRxiv - Genetics 2024Quote: ... 5 µl Proteinase K (20 mg/ml, Ambion) was added and the solution incubated for 1 hour at 50 °C ...
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bioRxiv - Physiology 2024Quote: ... 10-15 μg of placental homogenates or 5-10 μg of TPM were loaded onto a NuPAGE Bis-Tris Gels (Thermo Fisher Scientific), and electrophoresis was performed using MOPS-SDS running buffer at a constant 100 V for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... with the only difference that a 22 x 5 mm strip of PVC coverslips (ref 12-547, Fisher Scientific) placed vertically in the microtube before sealing with Aeraseal ...
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bioRxiv - Biochemistry 2021Quote: Synthetic genes encoding for human merlin FERM (22-312) and radixin FERM (5-295) domains were commercially synthesized (ThermoFisher) and cloned into pETM33 using NcoI and EcoRI restriction sites ...
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bioRxiv - Pathology 2024Quote: Staining was performed on 5-micron sections adhered to Superfrost Plus microscope slides (Fisher Scientific, cat # 22-037-246). Deparaffinization was conducted according to the manufacturer instructions for the commercial TUNEL assay with a series of baths starting with two 5-minute xylene baths followed by one 3-minute 50%:50% xylene-ethanol bath ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were further lysed via sonication (10 sec on 5 sec off, repeat 3x, ThermoFisher: FB120, 4°C) and protein was clarified by centrifugation at 21,000 x g for 5 min at 4°C ...
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bioRxiv - Genetics 2020Quote: ... supplemented with 15% fetal bovine serum and 5% HEPES (both Invitrogen).
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bioRxiv - Microbiology 2021Quote: ... Streptomyces hyphae were incubated with 0.5 mg/ml FM 4-64 Dye (N-(3-Triethylammoniumpropyl)24-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Molecular Probes) for 15 min in the dark ...
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bioRxiv - Microbiology 2022Quote: ... Cultures were normalized to optical density at 600 nm (OD600)=1 and centrifuged at 100xg for 20 minutes at 22 °C (Marathon 3000R, swinging bucket, Fisher Scientific). OD600 of supernatant was determined (BioMate 3 Thermo Spectronic ...
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bioRxiv - Neuroscience 2022Quote: ... The experiments were carried out at room temperature (20 – 22 °C) and cells were maintained in Live Cell Imaging Solution (Invitrogen, A14291DJ) that contains 140 NaCl,
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bioRxiv - Bioengineering 2021Quote: ... 5 × 10−5 M 2-mercaptoethanol (Gibco, 31350-010), 1X Minimal Essential Medium (MEM ...
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bioRxiv - Bioengineering 2021Quote: ... 5 × 10-5 M 2-mercaptoethanol (Gibco, 31350-010), 1X Minimal Essential Medium (MEM ...
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bioRxiv - Microbiology 2021Quote: ... We mixed an equal volume of the extracted LPS samples with an equal volume of 2× SDS buffer and electrophoresed 10 µl of each sample on Novex 4-20% plyacrylamide gradient gels (ThermoFisher) in Tris-Glycine-SDS buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples (10-20 μg) were run on SDS/PAGE with NuPAGE precast 4-12% gradient gels (Invitrogen; Carlsbad, CA, USA) and blots were incubated overnight at 4°C with primary antibodies (Supplementary Table 1 ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were heated for 10 min at 90°C and loaded on a 4–20% Tris-Glycine precast gel (ThermoFisher). Protein was transferred onto a nitrocellulose membrane using the iBlot Dry Blotting System ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 to 20 μg total protein was separated on a NuPAGE 4-12% Bis-Tris denaturing gel (Thermo Fisher Scientific) and transferred to a nitrocellulose membrane using an iBLot2 dry transfer (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were heated at 95 °Cfor 10 min and separated on 4–20% acrylamide Tris-glycine gradient gels (Life Technologies). Proteins were transferred to PVDF membranes (EMD Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... and samples were boiled at 95°C for 10 minutes and an equal amount of protein lysate (10-20 ug) was electrophoresed through 4–12% SDS-PAGE gels (Invitrogen) and then transferred to PVDF membranes ...
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bioRxiv - Physiology 2022Quote: ... and equal amounts (10 μg) were loaded into each lane and run on a 4-20% Tris-Glycine gel (Novex, Invitrogen) under reducing conditions ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled for 5 min and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) on either 10% gels (cast freshly) or 4-20% gradient gels (Invitrogen). Proteins were transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... and 20μg of total protein was loaded into each well of a 10-20% Tris-Glycine gel or a 4-12% Bis-Tris gel (Invitrogen). Protein was transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were precipitated at 5000g for 20 min at 4°C and resuspended in 10% fetal bovine serum (FBS, Gibco)-DMEM ...
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bioRxiv - Physiology 2024Quote: PDMS membranes subjected to stretch (0, 10, 20%) were fixed in 4% formaldehyde and stained with Alexa Fluor 488 Phalloidin (ThermoFisher) to visualize actin ...
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bioRxiv - Neuroscience 2024Quote: Western blotting was performed as previously described.7 Samples were boiled in 2X Laemmli sample buffer at 95°C for 10 minutes then run on pre-cast 4-20% Tris-glycine gels (Invitrogen). Samples were transferred to nitrocellulose membrane using iBlot 2 gel transfer device (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 ug of protein lysates/well were separated on 4-12% polyacrylamide gels (Thermo Fisher Scientific, MA, United States) at 100 V for 60 minutes and transferred to a Nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... 2 uL of 20 uM NLS-Cas9 were complexed with 4 uL of 10 uM sgRNAs in 14 uL Optimem (Invitrogen) for a final volume of 20 uL ...
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bioRxiv - Neuroscience 2022Quote: Deeply anesthetized 8-9 week old male and female C57BL/6JOlaHsd mice were perfused for 5 min with sulfo-NHS-biotin (4 mg/20 g mouse body weight, Thermo Scientific, cat no. 21217, dissolved in 20 ml PBS). Brains were dissected and handled as described under tissue preparation section.
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bioRxiv - Cell Biology 2024Quote: ... Sediments on the microscope slides were fixed in acetic acid/alcohol for 15 minutes and subjected to Epredia™ Papanicolaou EA Staining (22-050-211, Thermo Fisher Scientific, USA) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Sediments on the microscope slides were fixed in acetic acid/alcohol for 15 minutes and subjected to Epredia™ Papanicolaou EA Staining (22-050-211, Thermo Fisher Scientific, USA) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... and 100 µM copper sulfate (Fisher Scientific AC197722500). Click reactions were split into 50 µL reaction volumes and then incubated for 30 minutes in the dark at 25°C ...
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bioRxiv - Bioengineering 2023Quote: ... we washed the PA hydrogel three times with 1mL PBS and then stored it overnight at 4°C in PBS with 10% Antibiotic-Antimycotic 100X (Anti-Anti; Gibco, ThermoFisher, 15-240-062) and 1% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... 15 – 20 ng of RNA were reverse transcribed to cDNA (Superscript VILO, Invitrogen). cDNA and 10 ng of DNA were used for library preparation with the Ion AmpliSeq Library Kit Plus with a prostate specific custom multiplex RNA1 or DNA panel respectively and barcode incorporation (Ion Torrent ...
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bioRxiv - Cell Biology 2021Quote: ... Antibodies (15 μg) were conjugated to 20 μl protein L Dynabeads (ThermoFisher, 88849) before resuspending the washed beads with 1 ml of prepared lysate at ∼800 μg/ml and rotating for 2 h at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... interleukin-10 (IL-10) and interleukin-4 (IL-4) levels by sandwich ELISA kits (ThermoFisher Scientific). The measurement from unstimulated splenocytes (incubated with medium only ...
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bioRxiv - Genomics 2023Quote: ... 10-20% Tris-Glycine gels (Invitrogen #XP10205BOX) were used to resolve the samples ...
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bioRxiv - Biophysics 2024Quote: ... 10 % FBS and 20 mM HEPES (Gibco). In some instances live cell dyes ...
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bioRxiv - Microbiology 2021Quote: ... 4 μl of RNase A (20 mg/ml) (Invitrogen) was added and samples were incubated at 37°C for 15 min ...
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bioRxiv - Genomics 2023Quote: ... 4 ul of Proteinase K (20 mg/ml; Invitrogen) was added and incubated at 65℃ overnight ...
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bioRxiv - Microbiology 2023Quote: ... resolved on 4-20% Novex Tris-glycine gels (ThermoFisher), and used for immuno-blotting (via iBlot 2 gel transfer using transfer template program P0 ...
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bioRxiv - Microbiology 2022Quote: ... we used 4-20% Tris-acetate SDS gels (Invitrogen) as performed in (Yang et al. ...
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bioRxiv - Biochemistry 2024Quote: ... Novex 4-20% Tris-Glycine gels (WXP42020BOX, Thermo Fisher), Phusion High-Fidelity DNA polymerase (M0530L ...
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bioRxiv - Microbiology 2024Quote: ... Novex Tris-Glycine Mini Protein Gel 4-20% (Invitrogen), and MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Synthetic Biology 2023Quote: ... and stained for 10-15 minutes using SYBR Gold (ThermoFisher). Images were taken using an ImageQuant 400 Digital Imager (GE Healthcare) ...