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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... resolved using 4%-20% SDS-PAGE (Invitrogen), and analyzed by immunoblotting for the indicated proteins ...
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bioRxiv - Immunology 2022Quote: ... 4-20% Tris-glycine gels (Invitrogen #XPO420), and 0.45μm PVDF membrane (Thermo-Fisher #88518) ...
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bioRxiv - Cancer Biology 2021Quote: ... 15 μL 10% trifluoroacetic acid (Fisher Scientific®) was added to the lysate solution at a final concentration of 1% to stop digestion.
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bioRxiv - Molecular Biology 2024Quote: ... at 4°C for 24 h in 1.5 mL DMEM (Gibco #11995065) with dispase II (2.1 U/mL ...
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bioRxiv - Cell Biology 2021Quote: To prime the device 20 µL of L-15 media (Gibco) containing 20% hiFBS was pipetted through one of the two loading ports ...
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bioRxiv - Microbiology 2024Quote: ... b) H2 /CO2 atmosphere (80-20%) + trimethylamine (15 mM; ACROS organics); and c ...
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bioRxiv - Cell Biology 2023Quote: ... Stage 4 hippocampal neurons (5–10 DIV) were transfected with Lipofectamine 2000 (Thermo Fisher, Cat# 11668019). The following expression times were used ...
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bioRxiv - Immunology 2024Quote: ... 5×10-5 M 2-mercaptoethanol (Gibco) and 50µg/mL Gentamicin (Lonza ...
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bioRxiv - Genomics 2024Quote: 5-10 mg of each tissue sample (24 animals x 8 tissues) were used for Trizol-based RNA extraction (Invitrogen Cat# 15596026) following the manufacturer’s standard protocol (Supplementary Methods) ...
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bioRxiv - Genomics 2020Quote: ... 5 mM MgCl2, 1 mM EGTA, 0.5 mM DTT, 0.05% Tween-20, 4 U/mL SUPERase-In [Thermo Fisher] ...
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bioRxiv - Neuroscience 2024Quote: ... 0.05% Tween-20) overnight at 4 °C and with secondary antibodies diluted in 5% nonfat milk in TBST (ThermoFisher, A16078 [goat anti- mouse HRP ...
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bioRxiv - Cell Biology 2023Quote: ... or 24 hours before harvesting by resuspension in 1 ml Trizol (Thermo Scientific, 15-596- 026). A “0 hr” reference control was collected by performing a mock washout with media containing 100 nM GSI and immediately harvesting in Trizol.
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bioRxiv - Cell Biology 2021Quote: ... Passages were made before reaching confluence every 2-3 days by two washes with PBS (15 min each) and incubation in trypsin 0.05% for 5-10 min (Trypsin Cat#15400054, Thermo Fisher). MDCK cells stable transfected clones (MDCK-Shh ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 ug of total RNA or 15% of the immunoprecipitated samples was separated on a 10% TBE-Urea gel (Invitrogen), transferred to Zeta-probe (BioRad ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were seeded on Gold Seal coverglasses (22 × 22 mm2, no. 1.5; Thermo Fisher Scientific) and fixed with methanol for 10 min at −20°C ...
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bioRxiv - Neuroscience 2023Quote: ... 15-20 pooled day 30 neurospheres and 5-7 pooled day 60 organoids using TRIzol (Invitrogen; Thermo Fisher Scientific Inc., Waltham, MA, USA) and Direct-Zol RNA Mini Prep (Zymo Research ...
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bioRxiv - Physiology 2022Quote: 60 x 106 of mouse splenocytes were incubated for 15 min at 4°C in PBS containing 5% Normal Rat Serum (Thermo Scientific, 10710C) and anti-CD16/32 (BioLegend ...
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bioRxiv - Genomics 2021Quote: ... One OD600 absorbance unit (1.25 - 2.5 ml) of cells were collected and lysed at 95°C in 15 µL 4× NuPage LDS Sample Buffer (Thermo Fisher Scientific) with 5% β-mercaptoethanol for 10 minutes ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were cleared at 16,000g for 15 min at 4 °C and supernatants were supplemented with 5 μl/ml of RNase A/T1 (Thermo Fisher Scientific) for 30 min at 4 °C ...
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McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... (3) 65%-95% for 5 min and (4) washout for 15 min) using the EASY-nano LC 1200 system (Thermo Fisher Scientific) with a flow rate of 300 nL/min ...
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bioRxiv - Bioengineering 2024Quote: ... 10 µg of protein from each sample were loaded onto 4-20% gradient polyacrylamide gels (Novex™, ThermoFisher), and separated via electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: 10 µL (20 µg total protein) samples were separated on 4-12% Bis-Tris gradient gels (Invitrogen NW04122BOX) at 150V for 1 hour in MOPS SDS buffer (Invitrogen B0001.02) ...
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bioRxiv - Developmental Biology 2022Quote: ... All other lines were passaged at approximately 80% confluency (every 4-5 days on average) as tiny clusters (3-5 cells on average) using 0.5 mM EDTA (15575020, Gibco, concentration 10 mM).
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bioRxiv - Cell Biology 2021Quote: ... and then on day 20-22 media were replaced with DMEM (without glucose, with sodium pyruvate, Thermo Fisher Scientific) supplemented with Glutamax ...
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bioRxiv - Molecular Biology 2022Quote: ... We ligated both with an insert:vector ratio of 1:4 using Ligase (Thermo Scientific; 1 h, 22 C). The ligation mix was purified (Zymo Research ...
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bioRxiv - Evolutionary Biology 2024Quote: ... with 20 – 25 mg/L Ca2+ (CaCl2 * 2H2O, Fisher Bioreagents, Ottawa, CA) and 10 – 15 mg/L Mg2+ (MgCl2, Invitrogen, Vilnius, LT). We used this cation-adjusted MHB (CA-MHB ...
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bioRxiv - Molecular Biology 2024Quote: ... at 37°C for 2 hr and subsequently subjected to Proteinase K (15 μL 10% SDS, 7.5 μL 20 mg/mL Proteinase K (Thermo Fisher Scientific, 25530049)) digestion at 37°C for 2 hr ...
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bioRxiv - Molecular Biology 2020Quote: ... 10 mL of 10% Tween 20 (Fisher Scientific BP337500) and 850 mL ddH2O] under gentle agitation at room temperature ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells were passaged 1:4 into a new 15 cm dish and grown in DMEM supplemented with 10% FBS (Thermo Fisher) and Hygromycin (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... Between 1 and 3 milligrams of tissue was washed with 1X PBS buffer by vortexing for 15 minutes at 4°C and then sonicated on ice for 20 seconds using a Sonic Dismembrator M100 (ThermoFisher, San Jose, CA, USA). The homogenate was then centrifuged at 18,000 x g for 20 minutes at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... 22 mm × 22 mm) and large Petri dishes (Falcon™ Bacteriological Petri Dishes with Lid, 150 mm diameter, 15 mm height) from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: ... siRNA#4: 5’-AUAGCGUUUCUUCUAACUGGGCAGC-3’ (Invitrogen). siRNAs #2 and #4 significantly decreased the mRNA levels to the greatest extent and both had the same mitotic phenotypes ...
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bioRxiv - Systems Biology 2021Quote: ... 15% ZB-based fly extract and 5% penicillin/streptomycin (Gibco). Wing discs were loaded into the previously described REM-Chip (Narciso et al. ...
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bioRxiv - Biochemistry 2021Quote: ... and 5% Penicillin/Streptomycin (Thermo Fisher Scientific, 15-140-122) at 37°C in a 5% CO2-humidified incubator ...
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bioRxiv - Genomics 2021Quote: ... and then incubated with 5% or 15% Formamide (Ambion AM9342) in 2× SSC (5% formamide concentration was used for hybridization of Plasmodium probe libraries with low GC content) ...
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bioRxiv - Cancer Biology 2024Quote: ... anti-mouse CD45 PE-Cyanine 5 (Invitrogen 15-0451-82), anti-mouse EpCAM (CD326 ...
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bioRxiv - Microbiology 2021Quote: ... loaded equivalently in each lane (ranging from 4-20 μg between experiments) and run on a 4-20% Tris-Glycine gel (Novex, Invitrogen) under reducing conditions ...
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bioRxiv - Biophysics 2020Quote: ... The samples were incubated for 20 min at 4°C before they were loaded onto a pre-run 4-20% Novex TBE gel (Invitrogen). Electrophoresis was performed at 200 V for 45 min in TBE buffer (89 mM Tris ...
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bioRxiv - Physiology 2021Quote: HeLa cells and PC12 cells were seeded in 48-well plates at a density of 1×105 cells/well in 250 µL of RPMI 1640 medium (10% HS, 5% SFB and Ab-Am) grown for 24 h and transfected with Lipofectamine 2000 (Thermo Fisher, MA, USA) with 0.8 µg of the reporter plasmid (Gal4-dependent MH100tk-Luc) ...
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Mitochondrial bioenergetics stimulates autophagy for pathological tau clearance in tauopathy neuronsbioRxiv - Neuroscience 2024Quote: Cortical neurons at DIV10-14 were treated for 24 hours with 10 mM glutamine or 5 mM aspartic acid in Neurobasal-A medium (Invitrogen, Cat# A24775-01) supplemented with 1 mM pyruvate and 1 mM lactate ...
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bioRxiv - Cell Biology 2020Quote: ... cultured for 24 hours and transfected with 20 pmol siRNA using Lipofectamine RNAiMAX (Thermofisher). Transfected cells were cultured in normal medium for 24h and then serum-starved for 48h before analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... folates and porphyrins were relatively quantified while referencing an in-house library of chemical standards and using TraceFinder 4.1 (Thermo Fisher Scientific, Waltham, MA, USA), with a 5 ppm mass tolerance ...
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bioRxiv - Cell Biology 2020Quote: ... proteins were transferred onto a nitrocellulose membrane and then blocked overnight while shaken at 4 °C in TBST-5% BSA—a TBS Tween-20 (TBST; ThermoFisher) solution containing 5% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were suspended in 710 µl of RIPA buffer with 7.5 µl PIC and incubated with gentle mixing overnight at 4 °C with 20 µl of DynabeadsTM Protein G (Invitrogen) coated with antibody against the analysed protein (listed in Supplementary Table S2) ...
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bioRxiv - Neuroscience 2020Quote: Samples (5–10 µg protein) were typically run on 4–12% Bis-Tris gels (Life Technologies; WG1403BOX10) using MOPS buffer (NP0001 ...
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bioRxiv - Cell Biology 2021Quote: ... boiled for 5-10 min before the loading on 4-12 % Bis-Tris gels (Nu-PAGE, Invitrogen) and then transferred onto nitrocellulose membranes (GE Healthcare) ...
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bioRxiv - Cell Biology 2022Quote: ... the equivalent of 5-10 adult worms was loaded into 4-12% NuPAGE Bis-Tris gels (Invitrogen). Proteins were transferred overnight to nitrocellulose membranes ...
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bioRxiv - Pathology 2020Quote: ... OCT-embedded frozen specimens were cryosectioned at 5 μm onto charged slides (Fisher Scientific cat# 22-042-924). Slides were air-dried at room temperature overnight and fixed in 10% neutral buffered formalin for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and processed for 24 hours at 4 degrees with NeuroTrace (1:500 Invitrogen) in PBS containing 0.3% Triton-X ...
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bioRxiv - Neuroscience 2024Quote: ... and processed for 24 hrs at 4 degrees with NeuroTrace (1:500, Invitrogen) in PBS ...