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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for Copper;5 10 15 20 tetrakis 4 methylphenyl porphyrin 22 24 diide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 4 and 24 hours after treatment and the plasma membrane was stained with FM 4-64 from Life Technologies (100 μg ml-1). Cells were observed with an epifluorescence Leica DM6000 B research microscope with a 100 x magnification.
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bioRxiv - Cell Biology 2021Quote: ... Control and copper-supplemented cells were washed once with PBS and trypsinized for 5 min with 1x sterile trypsin solution (Thermo Fisher Scientific, USA). Cell suspensions were mixed with equal volume of 0.4% Trypan blue solution (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... β-mercaptoethanol denatured proteins (15-20 ug) were loaded onto 14% Tris-glycine polyacrylamide gels (ThermoFisher) and run at 120V for 90min ...
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bioRxiv - Molecular Biology 2023Quote: ... aegypti Aag2 cells (20) was cultured at 28°C in Leibovitz’s L-15 medium (Invitrogen) supplemented with filter-sterilized and heat inactivated 10% fetal calf serum (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: Total protein lysates (15–20 μg) were supplemented with NuPAGE LDS Sample Buffer (#NP0007, Invitrogen) including β-mercaptoethanol and separated with SDS-PAGE using NuPAGE 4–12 % Bis-Tris Midi Protein Gels (#WG1402BOX ...
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bioRxiv - Microbiology 2022Quote: ... of the samples were treated for 15 minutes at 55°C with 5 µL of proteinase K (10 mg/mL, Thermo Fisher Scientific, Waltham, MA, USA) prepared at 1 mg/mL in 100 µL of final volume and heat-inactivated at 98 °C for 5 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... A sample from each pull-down (5% of the total eluate) was separated by 4-20% SDS-PAGE and proteins were visualized using SilverQuest Stain (Thermo Fisher Scientific Life Technologies). Fractions containing a protein with the predicted molecular weight of the bait (usually fractions 2 and 3 ...
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bioRxiv - Neuroscience 2023Quote: ... or Tris-Glycine-SDS sample buffer (Novex) at 95°C for 5 min and separated using 4–20% Tris-glycine (ThermoFisher, #EC6025 or Invitrogen # XP04200). Proteins were transferred to nitrocellulose membranes (BioRad ...
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bioRxiv - Systems Biology 2020Quote: ... 5 μL of 20 mg/mL proteinase K (AM2548, Invitrogen) was added to the cells in lysis buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Then 20 μl of 5 mg/ml of MTT (Invitrogen) were added into each well for 4 h at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 0.1% Tween-20 (Thermo Fisher, #9005-64-5) at room temperature for 2 hr before overnight incubation with antibodies against phospho-ERKT202/Y204 (1:1000) ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 0.1% Tween-20 (Thermo Fisher, #9005-64-5) at room temperature for 2 h before overnight incubation with antibodies against γ-H2AX (1:400) ...
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bioRxiv - Genomics 2024Quote: ... 0.1% Tween-20 (ThermoFisher Scientific, cat. no. 9005-64-5), 0.1% IGEPAL CA-630 (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Recombinant wt and mutant αSyn were added to freshly prepared and sterile filtered electroporation buffer (EPB, 100 mM sodium phosphate, 5 mM KCl, 15 mM MgCl2, 15 mM HEPES, 2 mM ATP (Thermo Fisher #R0441), 2 mM reduced glutathione (Sigma # G4251 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Fifty μl of 10% w/v SDS and 5 μl of 20 mg/ml proteinase K (Thermo Fisher Scientific, catalog no. EO0491) were mixed with the cell suspension and the mixture was incubated at 57°C for 30 min ...
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bioRxiv - Immunology 2020Quote: Isolated splenic B cells (~10–20×106 cells) were stained first with 5 μL (5mM) Cell Trace Violet (C34557, Thermo Fisher Scientific) in 10 mL of PBS for 10 min at RT and let to recover in complete RPMI (+37°C ...
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bioRxiv - Biochemistry 2021Quote: ... followed by 10 cycles of 5’ 60 °C -> 5’ 24 °C (HDV cleavage of construct to release pure R3C) and DNAse I treatment (Thermo Scientific, USA). 75-85 nt randomised oligomer was expressed using artificial DNA template (Eurofins Genomics ...
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bioRxiv - Molecular Biology 2023Quote: ... The reaction mixture was left in a heatblock at 22 °C for 5 min before sampling every 30 s into a chilled quenching buffer (binding buffer from Thermo Scientific, K0701). To blunt the digested DNA ...
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bioRxiv - Cell Biology 2022Quote: ... 15 μg of protein were separated on NuPAGE 4-12% Bis-Tris gels (Invitrogen) before being transferred to nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by fixation for 15 min at room temperature in 4% paraformaldehyde (Thermo Fisher). Samples were acquired on an LSR-Fortessa (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... and 15 µl was run on a 4-12% Bis-Tris MOPS gel (Invitrogen) transferred to a PVDF membrane and was blocked with 1xPBST (0.1%Tween-20 ...
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bioRxiv - Microbiology 2020Quote: ... then 15 μg loaded onto a NuPAGE 4-12 % Bis-Tris polyacrylamide gel (Invitrogen) and separated by electrophoresis ...
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bioRxiv - Immunology 2021Quote: ... and 2.4 mm metal beads (4 beads/tube) (Thermo Fisher Scientific, 15-340-158). RNA was isolated by Phenol/Chloroform extraction and RNA yield determined using a NanoDrop Spectrophotometer (Nanodrop 2000c ...
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bioRxiv - Microbiology 2023Quote: ... sonicated via a Sonic Dismembrator 100 (Fisher Scientific, setting 3, 15 s, 4 pulses), and incubated on ice for 1 hr after the addition of 2 μL benzonase (EMD Millipore) ...
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bioRxiv - Immunology 2023Quote: ... cells were fixed for 15 min at room temperature in 4% paraformaldehyde (Thermo Fisher) after surface marker and viability dye staining ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were separated on WedgeWell™ 4–15% Tris-Glycine Gels (Invitrogen, Burlington, Canada) and transferred to 0.45 pore Immobilon-P PVDF membranes (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... and 15 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco, Gaithersburg, MD, USA). Cells were maintained at 37 °C in a humidified incubator with 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of protein were loaded in a 4-15% polyacrylamide gel (Thermo Fisher) and transferred to PVDF membranes and blocked with 5% dry-milk in TBST for 1 hour at room temperature ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples were centrifuged (4,500 g, 15 min, 4 °C; Multifuge X3R, Thermo Fisher Scientific), the supernatant was sterile filtered (0.22 μm ...
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bioRxiv - Immunology 2023Quote: ... samples were fixed for 15 min at room temperature in 4% paraformaldehyde (Thermo Fisher) after surface marker and viability dye staining ...
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bioRxiv - Microbiology 2020Quote: ... 4 μl of 5 mg/ml linear acrylamide (Ambion), 600 μl of preheated (65°C ...
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bioRxiv - Microbiology 2021Quote: ... siDDX42-4: 5’-AUCUCGAAUACCCUUUACG-3’ (ID:136410, Ambion®).
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bioRxiv - Cell Biology 2022Quote: ... and Ca2+-indicator Fluo-4/AM (5 μM, Invitrogen) in the presence of Pluronic F-127 (0.02% ...
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bioRxiv - Biochemistry 2021Quote: ... Hi-5 cells (BTI-TN-5B1-4) (Gibco #B85502) were cultured in Express Five™ SFM (Serum-Free Media ...
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bioRxiv - Neuroscience 2024Quote: ... 1∼5 mM in the Fluo-4(F14201, Invitrogen) form of Powder ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 1.5 μg of rRNA subtracted total RNA or 10 μg of in vitro-transcribed RNA was added to 4 μl 5× first strand buffer (ThermoFisher Scientific), 1 μl 10μM reverse primer ...
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Insights into the secondary structural ensembles of the full SARS-CoV-2 RNA genome in infected cellsbioRxiv - Biochemistry 2021Quote: ... 1.5 μg of rRNA subtracted total RNA or 10 μg of in vitro-transcribed RNA was added to 4 μl 5× first strand buffer (ThermoFisher Scientific), 1 μl 10μM reverse primer ...
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bioRxiv - Neuroscience 2020Quote: ... EV lysates corresponding to 5–10 µg of total protein were run into 4-12% Bis-Tris plus gels (Invitrogen, ThermoFisher Scientific). Prior to loading ...
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bioRxiv - Immunology 2023Quote: ... 20 ng ml-1 GM-CSF (Biozym) or X38-Ag8 supernatant (1%) and from day 5 on additionally with 10 ng ml-1 IL-4 (Life technologies). At day 7 ...
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bioRxiv - Molecular Biology 2022Quote: IP samples were eluted with 40 µl 1xLDS buffer adjusted to 10 mM DTT (5 min, 90°C) and separated on 4-12% Bis-Tris NuPAGE gels (Thermo Fisher) followed by colloidal coomassie staining (Instant Blue ...
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bioRxiv - Cell Biology 2023Quote: ... ESCs (5 × 10^4 cells/well) were dissociated into single cells with 0.5mM EDTA (15575020, Gibco; Thermo Fisher Scientific, MA, USA) after exposure to ROCK inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... ESCs (5 × 10^4 cells/well) were dissociated into single cells with 0.5mM EDTA (15575020, Gibco; Thermo Fisher Scientific, MA, USA) after exposure to ROCK inhibitor ...
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bioRxiv - Genetics 2023Quote: ... 20 mM EDTA) with 10 μL of 20 mg/mL Proteinase K (Thermo Fisher Scientific), 10 μL SDS (10% w/v) ...
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bioRxiv - Cell Biology 2024Quote: ... 20-30 μg protein was electrophoresed on a 10-20% SDS-PAGE gel (Invitrogen; XP10200BOX), transferred to a PVDF membrane (ThermoFisher ...
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bioRxiv - Cell Biology 2021Quote: ... with 10% (HEK293T, HeLa, Neuro2a) or 15% (C2C12) FBS (Thermo Fisher Scientific) in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Genetics 2020Quote: ... with 10% heat-inactivated FBS (15% for GM12878, HIFBS, Thermo Fisher Scientific), 2mM L-glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... Trypsinization was stopped using L-15 media containing 10% Horse Serum (Gibco). DRGs were then spun down at 1500 rpm for 5min ...
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bioRxiv - Systems Biology 2022Quote: ... supplemented with 10% FBS and 1% Penicillin-Streptomycin (Gibco, 15-140-122). All cells were cultured in a controlled humidified incubator at 37°C and 5% CO2 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10-15 mL of His-Pur Ni-NTA resin (Thermo Fisher Scientific) was used per L of cell culture ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were stabilized with 10-15 μg/ml blasticidin (Thermo Fisher Scientific) for two weeks and then maintained at 5-10 μg/ml blasticidin ...