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Citations for Thermo Fisher :
3401 - 3450 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... Freeze dried peptides were resuspended in 100 μL of distilled water and quantified by BCA assay (Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2020Quote: ... The solubilized peptides were then injected into a Q Exactive™ HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Scientific) with a Liquid Chromatography (LC ...
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bioRxiv - Molecular Biology 2019Quote: The kinetics of aggregation of Aβ40 (40μM) was measured by incubating the peptide with thioflavin T (ThT) (10 μM) (Fisher Scientific). The kinetics of aggregation of Aβ42 (10 μM ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting tryptic peptides were analysed by LC-MSMS using a Q Exactive coupled to an RSLCnano3000 (ThermoFisher Scientific). Raw files were processed using MaxQuant 1.5.2.8 using Andromeda to search a human Uniprot database (downloaded 03/12/14) ...
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bioRxiv - Physiology 2019Quote: Peptides were analyzed on a LTQ Orbitrap Velos with nano electrospray ionization source (Thermo-Fisher Scientific, San Jose, CA) connected to a 1100 Series HPLC (Agilent Technologies ...
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bioRxiv - Systems Biology 2020Quote: ... Peptide mixtures were analyzed by nanoLC-MS/MS using an Orbitrap Fusion mass spectrometer (ThermoFisher Scientific, Waltham, MA, USA). Protein identification was performed as described previously [56] with Proteome Discoverer v2.2 (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Immunology 2020Quote: ... predicted to have strong and weak binding to HLA-E*01:01 by the NetMHCpan 4.0 and Immune Epitope Database servers were synthesized using the PEPotec Immuno Custom Peptide Libraries service provided by Thermofisher Scientific (Table S3) ...
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bioRxiv - Bioengineering 2020Quote: ... 10 μL of 20-nM DNA origami structures (with different number of peptide attachment) in PBS were incubated with 0.1U/μL DNase I (Invitrogen) and 2mM MgCl2 for 25 min ...
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bioRxiv - Microbiology 2021Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Plant Biology 2021Quote: A total of 1 μg of re-suspended peptide was injected by an Easy-nLC 1000 system (Thermo Scientific) and separated on a Thermo Scientific™ EASY-Spray™ HPLC Column (75 μm x 500 mm ...
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bioRxiv - Pathology 2020Quote: ... Peptides were first loaded onto a trap column (PepMap C18, 2 cm x 100 μm x I.D.; Thermo Scientific) and then separated by an in-house packed analytical column (C18 ReproSil ...
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bioRxiv - Molecular Biology 2019Quote: ... Peptides analyzed by the Orbitrap Elite have been loaded on a C18 Acclaim PepMap100 trap-column (Thermo Fisher Scientific) with a flow rate of 30 μl/min 0.1% TFA ...
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bioRxiv - Cell Biology 2020Quote: Peptide samples were analyzed by nano LC-MS/MS on a Dionex UltiMate 3000 nanoRSLC chromatography system (Thermo Fisher Scientific / Dionex Softron GmbH ...
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bioRxiv - Biochemistry 2020Quote: ... Digested peptides were labeled using 11-plex or 16-plex tandem mass tag (TMT or TMTpro) reagents (Thermo Scientific), pooled ...
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bioRxiv - Synthetic Biology 2020Quote: ... Samples were then adjusted to 0.1% trifluoracetic acid and then desalted on Pierce peptide desalting spin columns (Thermo Scientific) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Peptides were concentrated on an Acclaim PepMap 100 C18 precolumn (75 μm x 2 cm, Thermo Scientific, Waltham, MA) and then were separated on an Acclaim PepMap RSLC column (75 μm x 25 cm ...
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bioRxiv - Microbiology 2019Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Biochemistry 2019Quote: ... Peptides were loaded with the autosampler directly onto a 2cm C18 PepMap pre-column (Thermo Scientific, San Jose, CA) which was attached to a 50cm EASY-Spray C18 column (Thermo Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... Peptides were eluted from the column and into the source of a Q Exactive Plus mass spectrometer (Thermo Scientific) using the following program ...
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bioRxiv - Neuroscience 2019Quote: ... The antigen peptide with N-terminus Cysteine (NH2-CGGGGAAPGPPRHG-COOH) was coupled with keyhole limpet hemocyanin (Thermo fisher 77600). The antigen was injected into rabbit and antisera were collected after primary and several booster injections ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were labeled for 1 hour at room temperature using 11-plex tandem mass tag (TMT) reagents (Thermo Scientific) resuspended in 40% acetonitrile ...
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bioRxiv - Immunology 2020Quote: ... Digested peptides were individually subjected to LC-MS/MS with 2hr gradients on an Orbitrap Fusion Lumos Tribid (ThermoFisher). MS/MS was acquired in data-dependent precursor selection (see Table S1 for MS/MS spectral counts per run) ...
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bioRxiv - Genetics 2020Quote: ... Purification of antibodies from serum was performed by coupling the respective peptides to SulfoLink™ coupling resin (Thermo Scientific) and following the manuals instructions ...
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bioRxiv - Genetics 2020Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMap 100, Thermo Scientific) with buffer A (0.1% formic acid in DDW ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the peptides were analyzed by LC−MS/MS using a Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Scientific) connected in-line to an Easy-nLC HPLC system (Thermo Scientific) ...
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bioRxiv - Physiology 2021Quote: ... Acidified samples were desalted in C18 desalting columns (Pierce Peptide Desalting Spin Columns, Thermo Fisher Scientific, Rockford, IL, USA) and reconstituted in 95% HPLC grade water ...
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bioRxiv - Cell Biology 2020Quote: ... peptides were analyzed by online C18 nanoflow reversed-phase nano liquid chromatography (Dionex Ultimate 3000 RS LC, Thermo Scientific) combined with orbitrap mass spectrometer (Q Exactive Orbitrap ...
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bioRxiv - Biochemistry 2021Quote: ... The dried peptides from each sample were reconstituted in 0.1% formic acid and quantified in a NanoDropTM (Thermo Scientific) to equalize all samples at an identical protein concentration before analysis on the liquid chromatography-tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Biochemistry 2021Quote: ... The peptide–mRNA fusions were then incubated with human and yeast DUBm-immobilized on Dynabeads M-280 streptavidin (Invitrogen) for 30 min at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... the trapped peptides were eluted onto a C18 column (nanoViper C18, 2 μm, 75 μm × 15 cm, Thermo Scientific) at a flow rate of 300 nL/min and subsequently separated with a 5-28% acetonitrile gradient with 0.1% formic acid for 80 minutes ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A’ (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Developmental Biology 2021Quote: ... and the next day peptides were labelled with TMT11plex reagents (0.4mg per sample) according to manufacturer’s instructions (Thermo Scientific). To quench the reaction ...
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bioRxiv - Cancer Biology 2020Quote: ... The resulting peptides were analysed by liquid chromatography mass spectrometry using an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Eluting peptides were on-line ionized by nano-electrospray (nESI) using the Nanospray Flex Ion Source (Thermo Fisher Scientific) at 1.5 kV (liquid junction ...
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bioRxiv - Biophysics 2021Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Eluting peptides were on-line ionized by nano-electrospray (nESI) using the Nanospray Flex Ion Source (Thermo Fisher Scientific) at 1.5 kV (liquid junction ...
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bioRxiv - Molecular Biology 2020Quote: ... The peptides were separated and then analyzed using a Q Exactive Plus Hybrid Quadrupole-Orbitrap mass spectrometer (ThermoFisher Scientific). The resulting MS/MS data were processed using the Mascot search engine (version 2.3) ...
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Targeting properdin - Structure and function of a novel family of tick-derived complement inhibitorsbioRxiv - Immunology 2021Quote: ... Digested peptides were analyzed by LC-MS/MS over a 30-min gradient using LTQ XL-Orbitrap (Thermo Scientific) at the CentralProteomicsFacility (http://www.proteomics.ox.ac.uk ...
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bioRxiv - Immunology 2020Quote: ... 750,000 PBMCs were incubated either with DMSO (negative control) or with different peptide pools in 0.5 ml RPMI (Gibco) +10% Human AB serum (Sigma ...
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bioRxiv - Immunology 2021Quote: ... incubated beads were rotated with elution buffer (50 mM Tris, pH 7.5, 500 mM NaCl, and 10 mg/ml Myc peptide (M2435, ThermoFisher)) for 60 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Peptides were loaded onto an Acclaim PepMap100 nanoViper C18 trap column (100 µm inner diameter, 2 cm; Thermo Scientific) in HPLC buffer C with a constant flow of 10 µl/min ...
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bioRxiv - Immunology 2020Quote: 250 μL K92 peptide at 1 mg/mL was reduced with 6 μL of 0.5 M Dithiothreitol (Thermo Fisher) for 40 minutes at 37 °C and alkylated with addition of 18 μL of 2-Iodoacetamide (Thermo Fisher) ...
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bioRxiv - Cell Biology 2021Quote: ... The eluted peptides were sprayed directly by electrospray ionization into the Q Exactive Plus Orbitrap mass spectrometer (Thermo Scientific). Mass spectra were acquired in data-dependent mode using a top10 sensitive method with one full scan (scan range ...
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bioRxiv - Cell Biology 2021Quote: ... Absolute abundances of Ub peptides were calculated by peak integration using Xcalibur Qual Browser (Version 2.2, Thermo Fisher Scientific). Layouts were applied according to the Supplementary Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Immunology 2022Quote: ... Peptides were loaded onto an Acclaim PepMap100 nanoViper C18 trap column (100 µm inner diameter, 2 cm; Thermo Scientific) in HPLC buffer C with a constant flow of 10 µl/min ...
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bioRxiv - Immunology 2022Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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An Azobenzene G-quadruplex Ligand Exhibits Promising Antibacterial Activity against Escherichia colibioRxiv - Microbiology 2022Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 10μg of peptide was prepared to label with TMTpro 16 plex reagents (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions ...