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Citations for Thermo Fisher :
3301 - 3350 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Then secondary antibodies were then added in blocking buffer over night at 4 ºC (Goat anti Rabbit 647, Thermo Fisher Scientific A21245 ...
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bioRxiv - Genomics 2024Quote: ... and pellets were resuspended in ice-cold bovine serum albumin (BSA) blocking solution (1× phosphate-buffered saline (PBS) (AM9625, Ambion), 0.5% BSA (VWR ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were also labeled with phalloidin conjugated to Alexa Fluor 594 in blocking buffer according to manufacturer protocol (Thermofisher A30107). After incubation with DAPI for 5 min and subsequent PBS wash ...
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bioRxiv - Immunology 2024Quote: ... The wells were then preincubated with either 200µl of BSA blocking buffer (Thermo Scientific; 5% bovine serum albumin in PBS) for total binding (TB ...
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Deep brain stimulation induces white matter remodeling and functional changes to brain-wide networksbioRxiv - Neuroscience 2024Quote: ... these sections were incubated for 24 h with blocking solution (5% normal goat serum (Cat #PI31873, Invitrogen, Waltham, MA, USA) and diluted with PBS solution containing 0.3% Triton X-100 (TX-100) ...
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bioRxiv - Cancer Biology 2024Quote: ... Followed by two more PBS rinses prior to 1 hour incubation with SuperBlock™ Blocking Buffer (Thermo Scientific™,37515) for blocking non-specific antibody binding ...
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bioRxiv - Biophysics 2020Quote: ... 1 nmol of biotinylated 4-heptad CTD peptides (PeptideSynthetics) were incubated with 0.5 mg of Streptavidin-coupled magnetic Dynabeads M-280 (Invitrogen) in 300 µL of buffer A (25mM Tris-HCl ...
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bioRxiv - Cancer Biology 2021Quote: Peptide fractions were analysed by nanoflow HPLC-MS/MS using an Ultimate 3000 nano RSLC system (Thermo Fisher Scientific) coupled with a Q-Exactive HF mass spectrometer fitted with a nano spray Flex ion source (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... the peptides were analyzed using nano flow reverse phase liquid chromatography on a Proxeon Easy-nLC 1000 (Thermo Scientific) coupled to an Orbitrap Elite (Thermo ...
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bioRxiv - Molecular Biology 2020Quote: ... Peptides were separated using an analytical C18 Acclaim PepMap column (75 µm × 250 mm, 2 µm vesicles; Thermo Scientific) and a 180-min gradient at a flow rate of 300 µl/min ...
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bioRxiv - Cell Biology 2019Quote: Fractionated peptides were separated by nanoscale C18 reverse-phase liquid chromatography using an EASY-nLC II 1200 (Thermo Scientific) coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2020Quote: ... Peptide yield from each sample was determined to be approximately 20 µg based on BCA Protein Assay (ThermoFisher Scientific).
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bioRxiv - Cell Biology 2020Quote: ... was used to elute peptides from the capillary reverse-phase column (0.075 × 500 mm. Pepmap®. Thermo Fisher Scientific). Data were acquired using the Xcalibur 4.0 software ...
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bioRxiv - Cell Biology 2020Quote: ... The digested peptides were dried in a SpeedVac and labelled with TMT10plex as instructed by the manufacturer (Thermo Fisher). Labeled peptides were mixed ...
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bioRxiv - Cell Biology 2020Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Microbiology 2020Quote: ... The peptides were separated by C18 reversed phase liquid chromatography using an EASY-nLC 1000 system (Thermo Fisher Scientific). A set-up of pre-column and analytical column was used ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The resulting tryptic peptide mixtures were separated using Thermo EASY nLC 1200 series system (Thermo Fisher Scientific, MA, USA) coupled online with a Thermo Orbitrap Fusion™ Mass Spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2022Quote: Peptides were measured using LC-MS instrumentation consisting of an Easy nanoflow HPLC system (Thermo Fisher Scientific, Bremen, Germany) coupled via a nanoelectrospray ion source (Thermo Fischer Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... LC-MS/MS analysis of the resulting peptides was conducted on a Q Exactive mass spectrometer (Thermo Fisher Scientific) coupled with an Easy-nLC1000 liquid chromatography system (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... 5µl of each peptide extracts were loaded onto 300 µm ID x 5 mm PepMap C18 precolumn (ThermoFisher, Dionex) at 20 µl/min in 2% acetonitrile ...
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bioRxiv - Cell Biology 2022Quote: For peptide analysis raw data processing and database searches were performed with Proteome Discoverer software 2.1.1.21 (Thermo Fisher Scientific). Peptide identifications were done with an in-house Mascot server version 2.5.1 (Matrix Science Ltd ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: Peptide fractions were analyzed by online nanoflow LC-MS/MS using a Proxeon easy-nLC 1200 system (Thermo Scientific) connected to a Q Exactive HF-X mass spectrometer (Thermo Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... Peptides were desalted (Acclaim PepMap 100, 75µm × 2 cm, nano viper, C18, 3 µm, 100 Å, ThermoFisher, Waltham, MA) and separated (Acclaim ...
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bioRxiv - Cell Biology 2019Quote: ... The tryptic peptides (∼1 µg loaded) were separated on a 50 cm × 75 µm (i.d.) column (Thermo Fisher Scientific) using a 120 min gradient of 12–45% acetonitrile ...
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bioRxiv - Microbiology 2019Quote: TMT-labelled tryptic peptides were subjected to HpRP fractionation using an Ultimate 3000 RSLC UHPLC system (Thermo Fisher Scientific) equipped with a 2.1 mm internal diameter (ID ...
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bioRxiv - Genetics 2019Quote: ... The identified proteins were filtered using the high peptide confidence setting in Protein Discoverer (version 2.2, Thermo Fisher Scientific), which correspond to a filter for 1% FDR on peptide level ...
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bioRxiv - Biochemistry 2019Quote: ... ATS-bound peptides were washed with 50 mM ammonium bicarbonate (3×) and eluted with 1 mM TCEP (Thermo Scientific) for 30 min at 37°C with constant rotation ...
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bioRxiv - Microbiology 2019Quote: ... The resulting peptides were subsequently labeled using a TMT-sixplex Isobaric Mass Tagging Kit (Thermo Scientific, Rockford, IL, USA) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: ... Peptides were trapped on a C18 column (75 μm inner diameter × 2 cm; nanoViper Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... LC-MS/MS peptide analysis was performed on a linear trap quadrupole (LTQ) Orbitrap XL mass spectrometer (Thermo Fisher) and coupled to an Ultimate 3000 RSLC Nano UHPLC System (Dionex) ...
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bioRxiv - Cell Biology 2019Quote: ... The resulting tryptic peptides were analysed by LC-MSMS using a Q Exactive coupled to an RSLCnano3000 (ThermoFisher Scientific). Raw files were processed using MaxQuant 1.5.2.8 using Andromeda to search a human Uniprot database (downloaded 03/12/14) ...
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bioRxiv - Immunology 2019Quote: ... Digested peptides were analysed by LC-MS/MS over a 30 min gradient using LTQ XL-Orbitrap (Thermo Scientific) at the Central Proteomics Facility (http://www.proteomics.ox.ac.uk ...
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bioRxiv - Cancer Biology 2020Quote: Desalted peptides from each sample (500μg) were labeled with 10-plex Tandem Mass Tag (TMT) reagents (ThermoFisher Scientific, 90110) (58) ...
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bioRxiv - Molecular Biology 2019Quote: ... 200 µg of total peptides per condition were labeled with tandem mass tag (TMT10plex and TMT11, Thermo Fisher Scientific). After >98% TMT incorporation rate was confirmed ...
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bioRxiv - Biochemistry 2021Quote: ... peptides in 1% (vol/vol) formic acid were injected onto an Acclaim PepMap C18 nano-trap column (Thermo Scientific). After washing with 0.5% (vol/vol ...
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bioRxiv - Biochemistry 2021Quote: Analysis of peptides was performed on a Q Exactive™ HF Hybrid Quadrupole-Orbitrap™ Mass Spectrometer (Thermo Scientific) coupled with a Dionex UltiMate 3000 RSLCnano Systems (Thermo Scientific) ...
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bioRxiv - Plant Biology 2020Quote: ... Peptides were identified using an EASY-nLC 1000 liquid chromatograph coupled to an Orbitrap Mass Spectrometer (Thermo Scientific, USA), followed by assessment using a SEQUEST algorithm embedded in Proteome Discoverer 1.4 software (Thermo Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... peptides were loaded on a C18 PepMap100 pre-column (300 μm i.d. x 5 mm, 100Å, Thermo Fisher Scientific) at a flow rate of 12 μL/min in 100% buffer A (0.1% FA in water) ...
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bioRxiv - Biochemistry 2020Quote: ... The modified peptide was then separated from the reaction mix on a Grace Vydac C18 column (Fisher Scientific, 218tp54) using a 0-70% acetonitrile gradient in 0.05% TFA at a flow rate of 0.5 mL/min ...
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bioRxiv - Biochemistry 2020Quote: Obtained peptides were reconstituted by 20ul of water containing 0.1% formic acid (FA) (Fisher Chemical, Thermo Fisher Scientific, Inc.). The proteomic analysis of the peptides was carried out on Q Exactive (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... Analysis of the on-line eluted peptides was performed using a QExactive HF-X mass spectrometer (Thermo-Fisher Scientific). The mass spectrometer operated in a data dependent mode with survey scans acquired at a resolution of 60,000 at m/z 400 ...
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bioRxiv - Cancer Biology 2021Quote: ... Peptides were analyzed by data-dependent MS/MS on a Q-Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific, MA). In brief ...
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bioRxiv - Cell Biology 2021Quote: Tryptic peptides were separated by nanoscale C18 reverse-phase liquid chromatography using an EASY-nLC 1200 (Thermo Fisher Scientific) coupled online to an Orbitrap Q-Exactive HF mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... a 77 amino acid length peptide with >98% purity as determined by analytical HPLC was synthesized by Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2019Quote: ... Peptide separation was performed on an EASY-nLC 1200 connected to a Q Exactive HF mass spectrometer (Thermo Scientific) using a 1 hr gradient consisting of Buffer A (0.1% FA in 2% acetonitrile ...
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bioRxiv - Biochemistry 2021Quote: Peptides were reconstituted in 0.1% formic acid (solvent A) and analyzed with a Dionex Ultimate 3000 UPLC (Thermo Scientific) coupled via a nanospray source to a Q-Exactive HFX tandem MS (Thermo Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... peptides were separated on an analytical column (2 μm Acclaim PepMap RSLC 75 μm x 25 cm, ThermoFisher Scientific) using a 150 min method and an effective gradient of solution A (1% ACN ...
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bioRxiv - Biochemistry 2020Quote: ... Digested peptides from HEK293 and HeLa cells were respectively injected into LTQ-Orbitrap Velos and Q Exactive (Thermo Fisher) onto a 75 μm i.d ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides (500ng) were eluted from an Acclaim Pepmap 100 trap column (75 micron ID x 2 cm, Thermo Scientific) onto an Acclaim PepMap RSLC analytical column (75 micron ID × 25 cm ...
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bioRxiv - Cell Biology 2021Quote: Peptide samples were analyzed by nano-LC-MSMS (DionexRSLCnano 3000) coupled to a Q-Exactive Orbitrap massspectrometer (Thermo Scientific). Briefly ...