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Citations for Thermo Fisher :
3401 - 3450 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... All cells were maintained in 1:1 Ham’s F12 Dulbecco’s modified Eagle’s medium (FDV) containing 5% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 5ml/500ml 100X penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific and goat anti-rabbit-HRP ...
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bioRxiv - Immunology 2021Quote: ... were diluted in two-fold dilution series from 1:5 to 1:5120 in Eagle’s Minimum Essential Medium (Gibco) with 5% FCS (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... primary antibody diluted in 5% NGS in PBS (β-catenin 1:200, ProteinTech, rhodamine-phalloidin 1:200, Molecular Probes) for 36 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were used at a ratio of 1:500 in 5% GS/1% BSA/0.2% PBST (anti-rabbit-Alexa-Fluor-555, Invitrogen A27039 ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 5% FBS and antibiotics (penicillin 100 U ml−1, streptomycin 100 U ml−1 (pen/strep) (Gibco), 10mM glutamax (Gibco ...
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bioRxiv - Systems Biology 2024Quote: ... 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1× SYBR green (Invitrogen) in TE buffer and mixed by pipette ...
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bioRxiv - Immunology 2020Quote: ... fraction was separated by centrifugation at 10,000 x g for 30 minutes at 2-8 °C (Sorvall Lynx 6000, ThermoFisher). The IB pellet was washed with 20 mM HEPES (pH 8.0) ...
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bioRxiv - Bioengineering 2023Quote: ... Whole conditioned media from flasks and bioreactors was 0.2 μm filtered prior to first round UC at an RCFmax of 133,900 x g (Sorvall WX ultraCentrifuge, ThermoFisher; Fiberlite F37L-8×100 Fixed-Angle Rotor, ThermoFisher, k factor=168 ...
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bioRxiv - Cell Biology 2020Quote: ... Human iPSC lines were maintained in 5% CO2 environment in Essential 8 Medium (Thermo Fisher). For cardiomyocyte differentiation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... P-gp-overexpressing subline KB-8-5-11 were maintained in DMEM (Thermofisher catalog #11965) with 10% FBS and 1% penicillin/streptomycin at 37°C in 5% CO2 ...
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bioRxiv - Genomics 2024Quote: ... solution (1mg L-Cystine, Sigma; 8 KU of DNase I, Affymetrix; in 5 ml DPBS), retina was incubated at 37C for ∼20min ...
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bioRxiv - Developmental Biology 2023Quote: ... with 5% FBS or at P7-8 in ice-cold Hibernate-A Medium (ThermoFisher, A1247501) with 10% FBS and B-27 Supplement (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: Cells were seeded onto 5 µg/ml laminin (BioLamina LN511) coated 8-chamber coverglass (Nunc™ Lab-Tek™ II Chambered Coverglass ...
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bioRxiv - Molecular Biology 2023Quote: The RNA of 500 µL iRBCs at 3-5% parasitaemia was isolated using 3 mL TRIzol reagent (Invitrogen) followed by phenol-chloroform phase separation ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged at 22,000 × g at 4°C for 20 minutes and poured over 4 mL of HisPur Cobolt resin (89964, Thermo Fisher Scientific). The slurry was rotated at 4°C for 1 hour and spun down at 700 × g for 2 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibody or IgG (as control) was added and incubated for 4 hrs at 4°C with Protein G Dynabeads (Life Technologies) for antigen coupling ...
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bioRxiv - Physiology 2021Quote: ... were immunostained for insulin (guinea pig anti-insulin pig from Abcam, 1:1000, overnight, 4’C, secondary antibody; Alexa Fluor 647-conjugated anti-guinea pig from Invitrogen; 1:500, 1 h room temperature) and nuclei were stained with DAPI (Sigma-Aldrich ...
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bioRxiv - Genetics 2024Quote: ... were incubated overnight at 4°C followed by the secondary antibodies (anti-rabbit 488, A11034 Invitrogen, 1:400; anti-mouse 555, A31570, Invitrogen, 1:400; DAPI, Roth, 1:400). Finally ...
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bioRxiv - Developmental Biology 2021Quote: Zebrafish fins or larvae euthanized in 1% Mesab were fixed in 4%PFA/1xPBS and embedded in 1% agarose (CryoStar NX50, Thermofisher) or tissue freezing medium (Leica ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated overnight at 4°C with the following primary antibodies at a concentration of 1:500: mouse anti-V5 (1:500, Invitrogen), rabbit anti-γ-H2AX (1:500 ...
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bioRxiv - Bioengineering 2019Quote: ... overnight at 4°C followed by AlexaFluor-568 (1:500, Invitrogen, A21099 and AlexaFluor-647 antibody (1:500, Invitrogen, A31573) respectively ...
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bioRxiv - Neuroscience 2020Quote: ... (iii) incubated overnight at 4 °C in the following affinity-purified rabbit KV7.2 (residues 1–70; 1:1000, Thermo Scientific), KV7.3 (residues 668–686 ...
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were resuspended in 1 mL of cold 1 X PBS (4°C) containing protease inhibitor (ThermoFisher; cat# 78429) and were transferred into tubes containing lysing matrix B (MP Biomedicals ...
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bioRxiv - Developmental Biology 2022Quote: ... with 0.1% (w/v) Tween20 and incubated overnight with primary antibodies at 4° C: anti-HIF1α (1:500, MA1-16504 Invitrogen); anti-STAT3 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS containing 0.3 % Triton X-100 and incubated overnight at 4°C with a rabbit anti-Iba1 antibody (1:500, Wako) in PBS containing 1 % FBS (Gibco), 0.1 % BSA (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Blot sections were probed overnight at 4°C 1:5000 with the ATXN1 antibody 11750 (Servadio et al., 1995) or 1:2500 with H1 antibody (Invitrogen) diluted in 5% milk PBST ...
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bioRxiv - Microbiology 2021Quote: ... clarified transfection supernatant was diluted 1/4 in a 20 μl volume of transfer buffer (Life Technologies Cat# NP0006-1) and applied dropwise to a presoaked 0.45 μm PVDF membrane ...
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bioRxiv - Biophysics 2021Quote: ... or ParB-EDTA buffer (by replacing the 4 mM MgCl2 with 1 mM EDTA) and then incubated with 1 μm diameter magnetic beads (Dynabeads, Myone streptavidin, Invitrogen) for 10 min ...
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bioRxiv - Plant Biology 2022Quote: ... A second round of reverse transcription was initiated with the addition of 4 µL of reverse transcription mix (4:2:1:1 of First Strand Buffer, 0.1 M DTT, RNaseOUT, SuperScript II; ThermoFisher Scientific) to each sample then incubated at 25□ for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26; 1:250, Invitrogen; Rabbit anti-Cx30 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were blocked in 5% normal goat serum + 2% BSA and 2% Triton-X for 1 h and incubated with the following primary antibodies overnight at 4°C: rabbit anti-GFP (1:1000, Invitrogen) and chicken anti-TH (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and then stained with HERV-K envelope primary antibodies (1:1000) (Ango) overnight at 4°C and Alexa Fluor 488 secondary antibodies (1:500) (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... was performed at 30V for 1 hour at 4°C in 1X NuPAGE™ buffer (REF NP0006-1, Life Technologies). After transfer ...
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bioRxiv - Microbiology 2022Quote: Loss of function of NEU1-4 was conducted by transfecting cells with pooled siRNAs (Supplemental Table 1) (Dharmacon) or single siRNAs (Supplemental Table 1) (ThermoFisher) using RNAiMAX (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were first incubated with rat anti-HA (1:100) antibody overnight at 4°C followed by donkey anti-rat Alexa Fluor (AF) 488 (1:500; Invitrogen) for 1.5 h at 20°C before staining for other markers ...
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bioRxiv - Physiology 2022Quote: ... Cell surface staining was done in live cells blocked with diluted superblock (1:4 dilution in PBS with 1 mM MgCl2 and 0.1 mM CaCl2, ThermoFisher Scientific) at 4°C for 0.5 h ...
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bioRxiv - Molecular Biology 2024Quote: ... mono-Methylated K35 overnight (1:100) at 4°C followed by 1:100 Alexa-Fluor 488 and Alexa-Fluor 555 (both Invitrogen) for 2 h at RT.
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bioRxiv - Cancer Biology 2024Quote: ... the Live/Dead assay was conducted using 1 μM calcein AM and 4 μM ethidium homodimer-1 (Life Technologies kit) for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 45 minutes with 1:500 AlexaFluor 488-conjugated anti-mouse (Invitrogen, 1:500 in 4% BSA PBS) plus DAPI (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... RPMI 1640 containing 2 μM calcein AM and 4 μM EthD-1 along with 1 mg/mL DAPI (ThermoFisher, 62248) was added to the 384-deep well plate (80 μL/well) ...
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bioRxiv - Bioengineering 2023Quote: ... 105 rested NY-ESO-1 TCR+ T cells (4:1 CD8+:CD4+) were labeled with 0.5uM CSFE (Thermo Fisher Scientific) and co-cultured with 105 NucLight red+ tumor cells in complete medium ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated for 2 hours at room temperature with secondary antibodies in PBS (Donkey anti-Mouse 647 nm 1:1000, Abcam ab15107, lot GR3234832-4; Donkey anti-Rabbit 594nm 1:1000, Invitrogen A21207 ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated overnight at 4 °C with the following antibody: ERα (Dilution 1:1 µg/mL; Invitrogen, CAT: MA5-13191). IRDye secondary antibody (Dilution 1:20,000 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with primary antibodies overnight at 4°C in a shaker (Cofilin Total 1:500 Invitrogen #MA517275; Phosphorylated-cofilin 1:500 Invitrogen #44-1072G and β-actin 1:3000 ...
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bioRxiv - Immunology 2023Quote: ... The upper aqueous layer was removed to a new tube and mixed 1:1 with Isopropanol (Fisher Scientific, BP2618-4) and incubated for 1 min with glycoblue co-precipitant (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were harvested by centrifugation at 4°C and resuspended in 40 mL 1:1 PBS:Bacterial Protein Extraction Reagent (BPER, ThermoFisher Scientific). Cell pellets were lysed by incubating in 60°C water bath for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... on day 6 and split 1:5 with a chemically defined neural medium including DMEM/F12 supplemented with GlutaMAX (1/1; Thermo-Fisher Scientific; Cat. No. 35050-061), Neurobasal medium (1/1 ...
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bioRxiv - Neuroscience 2019Quote: ... cortical cultures were loaded with fluo-4 AM (3 μM) or fura-2 AM (3 μM) plus 0.1% Pluronic F-127 (ThermoFisher) in a HEPES-buffered saline solution (HCSS ...
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bioRxiv - Developmental Biology 2021Quote: ... transfection medium was removed and replaced with 150 μl/well of DMEM with 4.5 mg/ml glucose and sodium pyruvate without L-glutamine and phenol red (w-G-SP, wo-G-PR) (Thermo Fisher Scientific) containing 10% FBS ...