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Citations for Thermo Fisher :
3351 - 3400 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Reactions were run on a Quantstudio 3 or 5 instrument (ThermoFisher). Cycling conditions for Quantifast reagents were ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5’- TTGGATCAGCTCAGACATATT-3’ or a nonspecific “scrambled” control (Invitrogen, United States). 72 hours after transfection ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were passaged every 3-5 days using TryplE (Gibco 12604054). Naïve and primed hPSCs were expanded and induced into different lineages in a 5% CO2 incubator at 5% O2 at 37C.
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bioRxiv - Neuroscience 2023Quote: ... or CRMP2 siRNA (5′ GTAAACTCCTTCCTCGTGT-3′; obtained from Thermo Fisher Scientific) using the 4D-Nucleofector (P3 Primary Cell Solution ...
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bioRxiv - Cell Biology 2023Quote: ... 3 days after transfection by 5 µl of lipofectamine 2000 (Invitrogen) with 2 µg of the plasmid and regularly re-sorted to maintain expression of myr/palm-mCherry.
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bioRxiv - Microbiology 2021Quote: ... biotinylated protein A and biotinylated protein G (4 μg/ml each) (Thermo Fisher Scientific) and Streptavidin-R-Phycoerythrin (4 μg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Cultures were fixed at DIV14 in 4% PFA and mounted using Fluromount-G (Invitrogen). A total of 3 independent cultures were generated per experimental condition ...
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bioRxiv - Cancer Biology 2019Quote: ... Chromatin was incubated overnight at 4°C with magnetic beads (Dynabeads Protein G, Invitrogen) and the following antibodies (4 µg each) ...
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bioRxiv - Plant Biology 2023Quote: ... overnight at 4 ᵒC and further incubated with protein-G Dynabeads (Thermo Fisher Scientific) for four hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG ...
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bioRxiv - Synthetic Biology 2022Quote: ... Samples were centrifuged (4,500 g, 15 min, 4 °C; Multifuge X3R, Thermo Fisher Scientific), the supernatant was sterile filtered (0.22 μm ...
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bioRxiv - Microbiology 2023Quote: ... and then rotated overnight at 4°C with 1.5 mg protein G (Dynabead, Invitrogen). Complexes were precipitated by centrifugation and washed three times in PBS supplemented 0.02% Tween 20 and resuspended in 100 uL PBS prior to elution with 40 uL of 2 parts 0.1M glycine pH 2.8 and 1 part NuPage sample buffer and reducing agent (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of 5 × Vilo reaction mix (Life Technologies, 11754), and 2 μl of 2 × Superscript III enzyme blend (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: Neutrophils were loaded with Fluo-4/AM (5 μM, Invitrogen) in the presence of Pluronic F-127 (0.02% ...
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bioRxiv - Developmental Biology 2019Quote: DFOs loaded with fluo-4 AM (5 μM, Molecular Probes) was performed with a two-photon microscope (Moving Objective Microscope ...
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bioRxiv - Immunology 2023Quote: ... High 5 cells (BTI-Tn-5B1-4; Thermo Fisher Scientific) were infected with virus particles ...
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bioRxiv - Developmental Biology 2022Quote: ... 7-amino-actinomycin (7-AAD, ThermoFisher, for labeling nonviable cells) stained cells were sorted using a FACS Aria II sorter (BD BioSciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were stained with 7-amino-actinomycin (7-AAD, ThermoFisher) to label nonviable cells and then sorted using a FACS Aria II sorter (BD BioSciences ...
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bioRxiv - Bioengineering 2023Quote: B-RBD cells (1 × 107 cells/mL) were combined with 4 μM Fluo-4 AM (Invitrogen, USA, Cat: #F14217) for 20 min at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:50,000 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated for one hour with AlexaFluor®-conjugated donkey secondary antibodies (1:500, Thermo Fisher Scientific) and finally counterstained with Hoechst 33342 (20 μm ...
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bioRxiv - Microbiology 2020Quote: SAV3 RNA from heart tissue was quantified with a modified one-step nsP-1 assay (Ag-Path, Ambion) (Jiraporn Jarungsriapisit et al. ...
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bioRxiv - Systems Biology 2019Quote: ... succinate and malate were quantified with either one of two HPLCs: 1) a Dionex Ultimate 3000 (Thermo Scientific) containing an LPG-3400SD pump ...
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bioRxiv - Molecular Biology 2023Quote: ... one volume of chromatin was mixed with 1/10 volume of Dynabeads M-270 Epoxy (ThermoFisher, LOT 01063817) coupled to immunoglobulin G (IgG ...
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bioRxiv - Bioengineering 2023Quote: ... One of the following goat-anti-mouse secondary antibodies was used: IgG Fc-HRP (1:10,000, Invitrogen A16084), IgG1 heavy chain HRP (1:10,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentration was measured spectrophotometrically (ε280 21430 M-1cm-1, MW 24.79 kDa) using NanoDrop One (Thermo Scientific). Dialysed MSP1D1 was concentrated to 5 mg/ml using a 10 kDa MWCO Amicon Ultra centrifugal concentrator (Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Biochemistry 2020Quote: ... at 37 °C with 5 % CO2 with 1 % penicillin/streptomycin (PS, 5000 U.mL-1 and 5000 μg.mL-1respectively; Life Technologies) and supplemented with 1% non-essential amino acids (Life Technologies).
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bioRxiv - Cell Biology 2021Quote: ... the stromal layer was washed with PBS and was subject to mild trypsinisation with 1:5 dilution of 0.25% Trypsin 1 mM EDTA (Thermofisher) to remove attached residual leukaemic cells before completely detaching stromal cells with 0.25% Trypsin-EDTA.
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bioRxiv - Systems Biology 2021Quote: ... Dried lipid fractions (~5 mg) were dissolved (isopropanol/dichloromethane 1:1, 300 μL) for accurate mass LC-MS (ThermoFisher Q exactive with Dionex Ultimate 3000 sample handler and Waters acquity UPLC BEH C18 with 1·7 μm particle size LC column ...
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bioRxiv - Genetics 2021Quote: ... PCR reactions contained 1 μl of 1:10 diluted lysate and 5 μl premixed AmpliTaq-Gold (0.3U, Life Technologies) with 0.26 μM of target-specific- and an additional fluorescent forward primer ...
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bioRxiv - Biochemistry 2020Quote: SHSY5Y cells were obtained from the Duke University Cell Culture Facility and were maintained at 37 °C with 5% CO2 in DMEM:F12 (1:1; Gibco) supplemented with 10% FBS (Sigma Aldrich ...
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bioRxiv - Plant Biology 2019Quote: ... Rh (10 µg L-1) and Ir (5 µg L-1) in 2% trace analysis grade (Fisher Scientific, UK) HNO3 ...
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bioRxiv - Physiology 2021Quote: ... Frozen tissues were mechanically homogenized with 1 stainless steel bead (5 mm) in 1 ml Trizol (Thermo Fisher Scientific) by shaking for 50 s at 30 Hz (TissueLyser ...
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bioRxiv - Genomics 2021Quote: ... nuclei were incubated for 1 hour in a solution containing 5% FBS and 1:500 primary antibody (H3K9me3; Invitrogen rabbit polyclonal 49-1008 or H3K9Ac ...
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bioRxiv - Cell Biology 2020Quote: ... All cells were maintained in 1:1 Ham’s F12 Dulbecco’s modified Eagle’s medium (FDV) containing 5% fetal bovine serum (FBS) (Thermo Fisher Scientific) and 5ml/500ml 100X penicillin-streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with secondary antibodies conjugated to HRP at 1:20,000 in 5% milk/1x TBST for 1 hour (goat anti-mouse-HRP, 32430; ThermoFisher Scientific and goat anti-rabbit-HRP ...
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bioRxiv - Immunology 2021Quote: ... were diluted in two-fold dilution series from 1:5 to 1:5120 in Eagle’s Minimum Essential Medium (Gibco) with 5% FCS (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... primary antibody diluted in 5% NGS in PBS (β-catenin 1:200, ProteinTech, rhodamine-phalloidin 1:200, Molecular Probes) for 36 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 5% FBS and antibiotics (penicillin 100 U ml−1, streptomycin 100 U ml−1 (pen/strep) (Gibco), 10mM glutamax (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies were used at a ratio of 1:500 in 5% GS/1% BSA/0.2% PBST (anti-rabbit-Alexa-Fluor-555, Invitrogen A27039 ...
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bioRxiv - Systems Biology 2024Quote: ... 1 μL of samples and 5 μL of standards were diluted into 95 μL of 1× SYBR green (Invitrogen) in TE buffer and mixed by pipette ...
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bioRxiv - Cell Biology 2019Quote: Analysis was performed using the Applied Biosystems validated TaqMan primer-probe set (Hs00208333/ spans exon 6 and 7) and the ABI PRISM SDS7000 sequence detection system (Applied Biosystems). The ΔCT method was used to quantify relative mRNA levels as described in ABI user guide ...
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bioRxiv - Immunology 2021Quote: ... FAM-ACACTAGCCATCCTTACTGCGCTTCG-BHQ) and PCR reactions were carried out on a QuantStudio 6 and 7 Flex Real-Time PCR System (Applied Biosystems). To generate a standard curve ...
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bioRxiv - Biochemistry 2021Quote: ... The grids were blotted for 6∼7 s at 100 % humidity and plunge-frozen in liquid ethane using a Vitrobot Mark IV instrument (Thermo Fisher). The RBD/Legobody complex at 2.5mg/ml were incubated with MS(PEG)12 methyl-PEG-NHS-ester (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the following thermal cycling conditions ...
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bioRxiv - Microbiology 2022Quote: ... Reactions were carried out in duplicate for samples and standards on the QuantStudio 6 and 7 Flex Real-Time PCR Systems (Applied Biosystems) with the thermal cycling conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.
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bioRxiv - Cancer Biology 2020Quote: ... Data analysis was performed with the QuantStudio 6 and 7 Flex Real-Time PCR System Software v1.0 (Applied Biosystems, Life Technologies) using the ΔΔCt method.