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Citations for Thermo Fisher :
3351 - 3400 of 8294 citations for Rat ERICH6 shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... and 20 μg sgRNA plasmid in Opti-MEM (Thermo Fisher Scientific) to a final volume of 1 mL ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmid (10 μg) and LipofectamineTM2000 (20 μL; Thermo Fisher Scientific) were added to 1 × 106 concetration of HEK293T cells ...
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bioRxiv - Cell Biology 2021Quote: ... TRPM7WT-sypHy or TRPM7LCF-sypHy plasmids using lipofectamine LTX (Thermo Fisher) or infected with sypHy ...
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bioRxiv - Biophysics 2020Quote: NSP1 expression plasmids were transformed into OneShot BL21(DE3) cells (Invitrogen) and grown overnight at 37 °C on LB agar plates supplemented with 50 µg/mL kanamycin ...
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bioRxiv - Cancer Biology 2021Quote: Plasmid transfections were performed in MCF7 cells using Lipofectamine2000 reagent (Invitrogen) according to the manufacturer’s directions ...
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bioRxiv - Cancer Biology 2022Quote: ... pSLIK-shMYC plasmids were transfected using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... These plasmids were then transfected into Min6 using Lipofcetamin 3000 (Invitrogen). Cells were selected with puromycin (0.15ug/ml ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were transfected using Lipofectamine LTX reagent (Thermo Fisher Scientific 5338030) and Opti-MEM (Thermo Fisher Scientific 31985070 ...
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bioRxiv - Cancer Biology 2022Quote: Plasmids were transfected into cells using Lipofectamine 3000 reagent (ThermoFisher Scientific) following a standard protocol ...
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bioRxiv - Physiology 2022Quote: ... cloned into a plasmid pCR 2.1-TOPO TA vector (Thermo Fisher) and transformed in thermocompetent DH5α Escherichia coli ...
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bioRxiv - Microbiology 2022Quote: ... All plasmids were amplified under ampicillin selection in Top10 cells (Invitrogen) and purified by NucleoBond Xtra Midi EF kit (MACHEREY-NAGEL).
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bioRxiv - Cancer Biology 2022Quote: ... Plasmids were performed via the lipofectamine™ 2000 transfection reagent (Invitrogen) according to the manufacturer ...
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bioRxiv - Cell Biology 2019Quote: The empty transfection control plasmid pcDNA3.1(-) was purchased from Life Technologies. The TfR-SNAP fusion construct was modified from mEmerald-TFR-20 (a gift from Michael Davidson ...
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bioRxiv - Genetics 2021Quote: ... together with the empty yeast plasmid pYES (Thermofisher, Waltham, MA, USA) as carrier DNA ...
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bioRxiv - Cell Biology 2021Quote: ... the plasmid has been dephosphorylated with FastAP Thermosensitive Alkaline Phosphatase (Thermofisher) and they have been ligated together with T4 DNA ligase (NEB) ...
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African Swine Fever Virus CD2v protein promotes β-Interferon expression and apoptosis in swine cellsbioRxiv - Microbiology 2020Quote: ... and plasmid DNA were separately diluted in Opti-MEM medium (Gibco) and incubated for 5 min ...
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bioRxiv - Molecular Biology 2020Quote: ... PlasmidSafe treated plasmid was transformed into Stbl3 competent cells (Thermo Fisher). LentiCRISPRCreb5gRNA plasmid containing the Creb5 gRNA was verified by sequencing (Genewiz) ...
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bioRxiv - Molecular Biology 2019Quote: Plasmid Pf86 was treated with BstBI restriction enzyme (Thermo Fisher Scientific) whose recognition sites are present 16 nucleotides upstream and 165 nucleotides downstream of the start codon of luciferase reporter gene ...
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bioRxiv - Biochemistry 2020Quote: ... The plasmids were transfected into Freestyle 293-F cells (Life Technologies) grown in suspension in FreeStyle293 medium ...
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bioRxiv - Neuroscience 2021Quote: Plasmids were constructed using the two-slot Gateway Cloning system (Invitrogen) and confirmed by restriction digest and sequencing.
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bioRxiv - Microbiology 2020Quote: Plasmids were cloned using Escherichia coli DH5α cells (Invitrogen, Karlsruhe, Germany). Construction of deletion mutants was performed by homologous recombination ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmids were linearized with the SacI restriction endonuclease (ThermoFisher Scientific, Canada) for 15 min at 37°C and used as templates for in vitro transcription ...
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bioRxiv - Immunology 2020Quote: ... Plasmids or siRNA were transfected into cells with Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2019Quote: ... Varying amounts of plasmid and lipofectamine transfection reagent (Thermo Fisher Scientific) were combined separately in 125 μL Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Transfections of plasmids were performed in triplicate with Lipofectamine 2000 (Invitrogen) as directed by the manufacturer with 10 pmol of siRNA (Control or On Target plus Smart Pool mouse FMR1 ...
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bioRxiv - Molecular Biology 2022Quote: ... or pcDNA5/FRT/TO-3XFL-ZFR and pOG44 plasmids (ThermoFisher Scientific) with TurboFect transfection reagent according to the manufacturer’s protocol (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: ... 2.5 μg of plasmid DNA and 5 μl P3000 reagent (Invitrogen) diluted in OPTI-MEM medium (Gibco ...
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bioRxiv - Systems Biology 2022Quote: Extracted plasmids were amplified using random hexamer primers (Thermo Scientific SO181) and φ29 DNA polymerase (NEB M0269L) ...
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bioRxiv - Cell Biology 2022Quote: ... or HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific, Waltham, MA) and sequenced to ensure that the correct modifications were in place.
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bioRxiv - Cell Biology 2022Quote: ... or a PureLink HiPure Plasmid Filter Midiprep Kit (Thermo Fisher Scientific). Primers were obtained from IDT (Integrated DNA Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were transfected with biosensor encoding plasmids using lipofectamine 3000 (ThermoFisher). Live-cell imaging was performed 48 hours post-transfection with a laser-scanning confocal microscope (FV1000 ...
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bioRxiv - Cell Biology 2022Quote: Transient transfection of plasmids was performed using Lipofectamine 2000 (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasmids were amplified using Subcloning Efficiency DH5a competent cells (Life Technologies). Plasmid DNA was isolated using the HiPure Plasmid Maxiprep kit (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: Cell transfection with plasmid DNA was performed using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... 6 µg plasmid DNA in Optimem® transfection medium (# 31985062, Gibco) was used for each transfection ...
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bioRxiv - Synthetic Biology 2020Quote: ... Plasmids and PCR products were purified using the GeneJET (Thermo Scientific) plasmid miniprep kit and gel extraction kit ...
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bioRxiv - Microbiology 2019Quote: ... Amplified DNA fragments were then ligated to pJET2.1 plasmid (Thermo Scientific), and the products transformed into E.coli DH5α ...
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bioRxiv - Biochemistry 2020Quote: ... All constructs were cloned into the pcDNA3.1(+)/hygro expression plasmid (Invitrogen). The Strep-FLAG pcDNA3.1(+)/G418 NEGR-1 and the anti-NEGR-1 primary antibody were kindly provided by Giovanni Piccoli (University of Trento ...
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bioRxiv - Cell Biology 2020Quote: ... EEA1 plasmids were generated using Gateway cloning system (Thermo Fisher Scientific), and EGFP-EEA1 plasmid as a template for cloning (Addgene ...
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bioRxiv - Developmental Biology 2019Quote: ... The PCR product was cloned into a pCR2.1-TOPO plasmid (Invitrogen) containing M13 primers and a T7 RNA polymerase transcription initiation site ...
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bioRxiv - Cell Biology 2019Quote: ... cells were transfected with the corresponding plasmid using Lipofectamine2000 (Life Technologies). Briefly ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA plasmids were mixed with Lipofectamine LTX (15338100, Thermo Fisher Scientific) or Fugene HD (E2311 ...
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bioRxiv - Biophysics 2019Quote: ... 1-5 µL (10pg-100ng) pRSET-EmGFP plasmid (Invitrogen, CA, USA) was mixed with 25 µL thawed bacterial solution and incubated for 5-10 minutes on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... inhibitor and the plasmids were transfected with lipofectamine 3000 (Invitrogen, USA). Briefly ...
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bioRxiv - Synthetic Biology 2020Quote: ... GeneJET gel extraction kit and plasmid miniprep kit (Thermo Fisher Scientific) were used for DNA extraction ...
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bioRxiv - Molecular Biology 2020Quote: ... and prepped using a HiPure plasmid Midi or Maxi kit (Invitrogen).
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bioRxiv - Synthetic Biology 2021Quote: All part plasmids were either directly synthesised (GeneArt, Thermo Fisher Scientific) or assembled as complementary single-stranded DNA oligos annealed together ...
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bioRxiv - Molecular Biology 2021Quote: Plasmids were transfected cells using Lipofectamine 2000 Reagent (11668-019, Invitrogen), FuGENE HD Transfection Reagent (E2311 ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting sgRNA plasmid was linearized by FastDigest DraI (Thermo Scientific) and served as template for sgRNA in vitro transcription using the T7 MEGAshortscript Kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmid DNA and siRNA oligonucleotides were transfected using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s instructions ...