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Citations for Thermo Fisher :
3351 - 3400 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: RT-qPCR: We synthesized first-strand cDNA with M-MLV reverse transcriptase (Life Technologies), and performed qPCR with PowerUp SYBR Green Master Mix (ThermoFisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... and 200 μl of 0.5 M pH 8.0 EDTA (ThemoFisher 15575020) into DPBS (Gibco), vacuum filtering with a 0.22-μm filter (Millipore) ...
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bioRxiv - Physiology 2021Quote: ... cDNA was generated by RNA reverse transcription using M-MLV Reverse Transcriptase (Thermo Fisher). Quantitative real-time PCR (qPCR ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and coated with Shandon M-1 embedding matrix (Thermo Fisher Scientific, Kalamazoo, MI, USA) in preparation for sectioning ...
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bioRxiv - Microbiology 2021Quote: ... The BMDMs were lysed using mammalian protein extraction reagent (M-PER, Thermo Scientific®) with added cOmplete ...
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bioRxiv - Bioengineering 2020Quote: ... followed by immediate neutralization with 1 M Tris-HCl pH 8 (Thermo Fisher Scientific). Following elution from the column ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated at RT with 0.5 M glacial acetic acid (Fisher Scientific #BP1185-500) for 20–60 min and washed once with sterile milliQ (MQ ...
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bioRxiv - Genomics 2020Quote: ... each well of cells were lysed with 100 μL M-per buffer (ThermoFisher Scientific).
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bioRxiv - Immunology 2021Quote: ... The color reaction was stopped with 50 μL/well 4 M H2SO4 (Thermofisher Scientific). Optical density (OD ...
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bioRxiv - Immunology 2021Quote: ... Prior to electrophoresis the proteins were reduced with 0.1 M DTT (Thermo Fisher Scientific) and heat denatured at 95°C for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... and 5μg/ml insulin and 10−8 M dihydrotestosterone (Corning, Fisher Scientific, Waltham, MA). Cells were treated with 100μM ATP (A2383 ...
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bioRxiv - Cancer Biology 2022Quote: The total protein fraction was extracted using M-PER buffer (Thermo Fisher Scientific, USA) with protease and phosphatase inhibitors (Sigma Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... the media was switched to low glucose DMEM (Gibco, Thermo Fisher, Waltham, M. 11885084) supplemented with 0.01% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2022Quote: ... the media was switched to low glucose DMEM (Gibco, Thermo Fisher, Waltham, M. 11885084) supplemented with 0.01% bovine serum albumin (BSA ...
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bioRxiv - Bioengineering 2022Quote: ... fresh bromoacetylation reagent was made by dissolving 1.2 M bromoacetic acid (>99%, Fisher Scientific) in dimethylformamide (DMF ...
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bioRxiv - Cell Biology 2022Quote: mRNA expression was determined as described previously34 using M-MLV Reverse Transcriptase (Invitrogen, 28025). The primers are detailed in Table S4.
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bioRxiv - Cell Biology 2022Quote: Frozen brown adipose tissue was homogenized in 150 µL cold M-Per (Thermo Fisher) containing 1:50 HALT Protease and Phosphatase Inhibitor Cocktail (EDTA-free ...
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bioRxiv - Immunology 2022Quote: ... Cells were lysed with 0.5 ml of 0.5 M NaOH (Fisher Scientific, SS255-1) and neutralized with 0.5 ml or 0.5 M HCl (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from skeletal muscle (m. quadriceps femoris) using TRIzol reagent (Invitrogen) and purified using RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were harvested and lysed in M-per mammalian protein extraction reagent (Thermo scientific) with protease inhibitor (EDTA-free ...
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bioRxiv - Microbiology 2023Quote: ... cells were lysed using the M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) following manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2024Quote: ... whole cell extracts were collected by using M-PER lysis buffer (Thermo Fisher Scientific) supplemented with protease inhibitor and phosphatase inhibitor cocktail (Merck Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... reduced and alkylated with 1.4 μl of 0.5 M tris2(-carboxyethyl)-phosphine (Thermo Fisher) and 7 μl of 0.4 M chloroacetamide in a 90 °C water bath for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was reverse transcribed using a M-MLV reverse transcriptase kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and biotin-labeled DNA was pulled down with Dynabeads M-280 Streptavidin (Invitrogen, #11205D). Library preparation was performed using a DNA library preparation kit (Vazyme ...
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bioRxiv - Cell Biology 2023Quote: ... Whole cell extracts were isolated using the M-PER kit (Thermo Fisher Scientific, USA) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... and a CD-5MS column (30 m x 0.25 mm x 0.25 μm) (ThermoFisher).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The pH was adjusted by adding either 0.1 M HCl (Fisher Scientific, A144S-500) or 1 M NaOH (Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Magnetic beads (11204D, Dynabeads M-280 sheep anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific) were pre-blocked with BSA (1 mg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Magnetic beads (11204D, Dynabeads M-280 sheep anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific) were pre-blocked with BSA (1 mg/ml ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was converted into complementary DNA (cDNA) using M-MLV reverse transcriptase (Invitrogen) and random hexamers ...
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bioRxiv - Genetics 2023Quote: ... an aliquot of 75 µL of M-270 Streptavidin Dynabeads (Thermo Fisher Scientific, 65305) were prepared by washing three times and resuspended with 200 µl of binding buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... cell lysis was performed by adding M-PER lysis buffer (Thermo Scientific; Rockford, IL) supplemented with 1x cOmplete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Biophysics 2023Quote: Carboxylate functionalized 2.8 μm superparamagnetic beads (M-280 Dynabeads, Thermo Fisher Scientific, Waltham, MA) were coated with protein A (Prospec ...
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bioRxiv - Biochemistry 2023Quote: ... Target-bound complexes were recovered using M-270 Streptavidin beads (Invitrogen, Carlsbad, CA, USA). Stringent selection of high-affinity binders was achieved by up to 6 washing steps with PBSMT and competitive elution of the immobilized complexes using decreasing concentrations of non-biotinylated target 67B89 in the last two cycles of RD ...
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bioRxiv - Neuroscience 2023Quote: Human fibroblasts were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific) supplemented with cOmplete protease and PhosSTOP phosphatase inhibitors (Roche) ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were prepared in M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, Cat#78501) with β-mercaptoethanol (0.5 mM ...
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bioRxiv - Cancer Biology 2023Quote: ... then reduced using 4 µ M (final concentration) of TCEP (Thermo Fisher Scientific, 77720) to reduce the thiol groups for 30 min in a 37 °C water bath ...
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bioRxiv - Biochemistry 2023Quote: Cells were lysed by M-PER mammalian protein extraction reagent (Thermo Scientific, Cat# 78501), protein quantified by BCA protein assay ( Thermo scientific #23250) ...
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bioRxiv - Genetics 2023Quote: ... 4 µL of pre-washed rabbit IgG-conjugated M-270 Epoxy Dynabeads (Invitrogen, #14311D) were added and lysates were incubated on a wheel for 1 hour at 4°C ...
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bioRxiv - Genomics 2023Quote: ... 30 μl of M-270 streptavidin magnetic beads (catalog num. 65305, Thermo Fisher Scientific) were washed twice with 30 μl of CapTrap LiCl binding buffer (c3.64 ml of nuclease-free water ...
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bioRxiv - Genomics 2023Quote: ... 1 M NaCl) and then suspended in 1 × SuperScript II First Strand Buffer (Invitrogen) supplemented with SUPERase•In™ RNase Inhibitor (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... 500 ng of total RNA were reverse-transcribed using M-MLV reverse transcriptase (Invitrogen) with random hexamer primers ...
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bioRxiv - Cell Biology 2022Quote: ... 2 µL 1 M DTT and 27.5 µL of T4 Polynucleotide Kinase (Thermo Scientific) were added to the annealed oligonucleotides and the reaction was incubated for 2 h at 37 °C ...
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bioRxiv - Biophysics 2023Quote: ... 35 µl of amine-labeled magnetic beads (Thermo Fisher Scientific, M-270 amine, 14307D) was washed with buffer A (0.1 M sodium phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... Annealed oligos were bound to Streptavadin M-280 Dynabeads (Thermo Fisher, reference number 11205D), in annealing buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 27 µl of diluted 1 M Tris-HCl (pH 8.0) (Invitrogen #15568-025) were simultaneously added to each well ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesised from 1 µg of RNA with M-MuLV reverse transcriptase (Invitrogen) and random hexamers as specified by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... and incubated with 1 mg of streptavidin-coated magnetic beads (Dynabeads M-270, Invitrogen) for 2 h at 370 C in 400 μl of BW buffer (5 mM Tris–HCl pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... were cross-linked to Dynabeads M-270 Epoxy (Thermo Fisher Scientific, catalog no. 14311D) for immunoprecipitation ...