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Citations for Thermo Fisher :
3301 - 3350 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... and retro-transcribed by using 100 units of M-MLV Reverse transcriptase (Life Technologies) in the presence of 1.6 µM oligo(dT ...
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bioRxiv - Cell Biology 2020Quote: Whole-cell lysates were prepared in M-PER mammalian protein extraction reagent (ThermoFisher Scientific) containing a Halt™ protease inhibitor cocktail (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... These Peptides (35 µM) were incubated with Dynabeads™ M-280 streptavidin (Invitrogen # 11205D) in binding buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Developmental Biology 2020Quote: ... 1/10th volumes of 3 M Sodium Acetate and 30 μg Glycoblue coprecipitant (Ambion).
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bioRxiv - Molecular Biology 2020Quote: ... 30 μL 0.1 M DTT (SuperScript™ III Reverse Transcriptase Kit, Invitrogen Cat #18080044), 45 μL 1 M Tris-HCl (pH 8.0 ...
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bioRxiv - Molecular Biology 2021Quote: ... The protocol of preparing the IgG-Dynabeads from Dynabeads M-270 Epoxy (Thermo Fisher) was taken from (53) ...
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bioRxiv - Molecular Biology 2021Quote: ... The protocol of making the IgG-Dynabeads from Dynabeads M-270 Epoxy (Thermo Fisher) was taken from (53) ...
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bioRxiv - Genetics 2019Quote: ... First strand cDNA was synthesized using M-MuLV Reverse Transcriptase (RT) (Life Technologies, Invitrogen). Briefly ...
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bioRxiv - Genetics 2019Quote: ... First strand cDNA was synthesized using M-MuLV Reverse Transcriptase (RT) (Life Technologies, Invitrogen). Briefly ...
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bioRxiv - Plant Biology 2020Quote: ... DNA fragments labeled by biotin were separated on Dynabeads M-280 Streptavidin (ThermoFisher, USA), then purified ...
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bioRxiv - Molecular Biology 2019Quote: ... cDNA was synthesized by use of random hexamer (M-MLV reverse transcriptase, Invitrogen, 28025013). Quantitative real-time PCR was carried out by using QuantiTect SYBR Green kits (Qiagen ...
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bioRxiv - Plant Biology 2019Quote: ... 30 m × 0.25 mm ID × 0.25 μm (Thermo Fisher Scientific Inc., Waltham, MA, USA) was used as a column ...
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bioRxiv - Cell Biology 2019Quote: ... Aliquots of 500 ng RNA were reverse-transcribed by M-MLV reverse transcriptase (Invitrogen) with random hexamers ...
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bioRxiv - Cell Biology 2019Quote: ... from a 1 M stock in H2O in glucose free DMEM (11966025, ThermoFisher Scientific) supplemented with 10% FCS for 1 h (glycolysis stress) ...
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bioRxiv - Immunology 2019Quote: ... The L-M fibroblasts were grown in DMEM (Gibco, high glucose, pyruvate, #41966-029) supplemented with 10% FBS (Gibco ...
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bioRxiv - Biochemistry 2021Quote: ... 1 µL PIC and 9 µL of Dynabeads™ M-280 Streptavidin (ThermoFisher Scientific) were added to each output (‘beads’ ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysis was performed in a French Press Cell G-M™ (ThermoFisher Scientific) and the recombinant protein was purified by gravity-flow chromatography using a nickel-charged resin Ni-NTA Agarose (QIAgen ...
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Convergent evolution of distinct immune sensor systems for fungal polygalacturonases in BrassicaceaebioRxiv - Plant Biology 2020Quote: ... First strand cDNA was synthesized with RevertAid™ M-MuLV reverse transcriptase (Thermo Scientific) and qRT-PCR was performed with the iQ5 Multi-color real-time PCR detection system (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... was conjugated to Dynabeads M-280 sheep anti-mouse IgG beads (Invitrogen, catalog #11201D). For FLAG ChIP-seq (for Brn1 and Hho1) ...
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bioRxiv - Molecular Biology 2020Quote: ... 15 μl Dynabeads M-280 Sheep Anti-Mouse IgG (Invitrogen, Carlsbad, California, United States) per sample were washed twice in 1x BrU-IP buffer (20 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA was synthetized from 1 mg of total RNA using M-MLV RT (Invitrogen). cDNA samples from 1:5 dilutions were used for real-time PCR reactions ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was converted to complementary DNA (cDNA) using M-MLV reverse transcriptase (Invitrogen) and random hexamers ...
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bioRxiv - Microbiology 2020Quote: THP-1 cells were lysed in M-PER mammalian protein extraction reagent (Thermo Scientific) supplemented with Pierce protease inhibitor (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: Oligo pulldown was performed according to the manual for DynaBeads M-280 Streptavidin (ThermoFisher). In short ...
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bioRxiv - Cancer Biology 2021Quote: ... and resuspended in mammalian protein extraction reagent (M-PER; Thermo Scientific, Rockford, IL, USA) supplemented with protease and phosphatase inhibitor cocktail (PPI ...
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bioRxiv - Cell Biology 2021Quote: ... the M- and N-gene were partially cloned into pCR™2.1 (ThermoFisher Scientific) or pMiniT 2.0 (NEB) ...
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bioRxiv - Microbiology 2022Quote: ... total RNA was first converted to cDNA using the M-MLV reverse transcriptase (Invitrogen) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... 7M Urea buffer supplemented with Mammalian Protein Extraction Reagent (M-PER) (Thermo Scientific, USA) and 1X protease inhibitor cocktail was used to lyse the cells ...
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bioRxiv - Immunology 2022Quote: ... The cDNA synthesis was processed using Random hexamers and M-MLV Reverse Transcriptase (Invitrogen). BIO-RAD CFX284TM Real-Time PCR Detection System was used for SYBR green-based quantitative PCR ...
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bioRxiv - Immunology 2022Quote: ... CA) were filled with rhIL-34 (0.42µg/h, i.e. 0.4 m/kg/d; Thermofisher, Waltham ...
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bioRxiv - Biochemistry 2022Quote: ... the pellet was treated with Mammalian Protein Extraction Reagent (M-PER) (Thermo Scientific, USA) along with 1X protease inhibitor cocktail (PIC) ...
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bioRxiv - Microbiology 2020Quote: ... The ACE2-SBP was pull-down by 10µl Dynabeads M-270 Streptavidin (Thermo Fisher) for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... flow-aligned cells were lysed with M-PER mammalian protein extraction reagent (Thermofisher, MA) supplemented with 1% protease and phosphatase inhibitor cocktail (Thermofisher ...
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bioRxiv - Cell Biology 2021Quote: Cells were lysed in M-PER™ Mammalian Protein Extraction Reagent (Thermo Scientific, Waltham) containing a protease inhibitor mixture (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2021Quote: ... tissues were sectioned on a M 355S rotary microtome (ThermoFisher Scientific, Hanover Park, IL) at 4 μm thickness and mounted on glass slides ...
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bioRxiv - Cancer Biology 2020Quote: Cells were lysed using M-PER™ Mammalian Protein Extraction Reagent (78503, Thermo Scientific) supplemented with Halt™ Protease Inhibitor Cocktail (87785 ...
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bioRxiv - Biochemistry 2020Quote: ... Labeled Protein A (200 µg) was then conjugated to Dynabeads M-280 Tosylactivated (Invitrogen) following the provided protocol to produce 10 mg of beads at a final concentration of 20 mg/mL ...
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bioRxiv - Microbiology 2021Quote: 500.000 cells were lysed in M-PER Mammalian Protein Extraction Reagent (Thermo Scientific 78501) containing 1x protease inhibitor (cOmplete Tablets ...
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bioRxiv - Bioengineering 2021Quote: ... DNA-immobilization on magnetic beads (Dynabeads M-280, mean diameter 2.8 µm, Thermofisher, US) was performed following the same protocol.
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bioRxiv - Biophysics 2021Quote: 20 µl of streptavidin coated Dynal Dynabeads M-270 streptavidin coated magnetic beads (ThermoFisher) was mixed with ∼0.1 ng of RNA hairpin (total volume 40 µl ...
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bioRxiv - Developmental Biology 2019Quote: ... 200 μM each deoxynucleotide triphosphate [dNTP]) using 200 U M-MLV Reverse Transcriptase (Invitrogen) in 20 μl of a reaction mixture ...
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bioRxiv - Genomics 2019Quote: ... Dynabeads M-280 sheep anti-rabbit IgG beads (Thermo Fisher Scientific, Cat. No. 11203D) were used for immunoprecipitation ...
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bioRxiv - Molecular Biology 2019Quote: ... pre-coupled to magnetic beads (Dynabeads M-280 Sheep anti-Rabbit IgG, Thermo Fisher). A series of wash steps was employed to ensure stringency ...
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bioRxiv - Cell Biology 2019Quote: ... and supernatant was incubated overnight at +40C with anti-mouse IgG M-280 (ThermoFisher) dynabeads previously incubated with anti-Ser5P Pol II mouse antibodies (ab5408 ...
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bioRxiv - Cancer Biology 2019Quote: Cells were lysed on-plate using Mammalian Protein Extraction Reagent (M-PER; Thermo Scientific), with Halt Protease Inhibitor Cocktail (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2020Quote: ... First-strand DNA synthesis was performed using M-MLV Reverse Transcriptase (Invitrogen, Darmstadt, Germany) and random primers according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... HBECs were lysed in Mammalian Protein Extract Reagent (M-PER; Thermo Scientific, Rockford, 78501) with sample buffer solution (Wako ...
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bioRxiv - Biochemistry 2020Quote: RNA was isolated from livers homogenized in Buffer M using Trizol (Invitrogen, USA, 15239794), cDNA prepared using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Genomics 2020Quote: ... The reaction was stopped by addition of 12 μl 0.5 M EDTA (Invitrogen, #15575038) at 65°C for 20 min ...
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bioRxiv - Molecular Biology 2021Quote: AF488-Col was mixed 1:1 with 1 M acetic acid (AC124040025, Acros Organics) containing 1.3 M sodium chloride to precipitate the collagen[46] ...