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Citations for Thermo Fisher :
3151 - 3200 of 6840 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... was applied for two h at room temperature prior to washing and coverslipping with Prolong Gold (Invitrogen #P36931) containing DAPI ...
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bioRxiv - Neuroscience 2021Quote: ... The brains were dissected and post-fixed for 72 h in fresh 4% PFA (Thermo Fisher Scientific #28906) before being embedded in low melting point agarose (Thermo Fisher Scientific #R0801 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... for 2 h at room temperature and slides were mounted and counterstained with Fluoromount with DAPI (ThermoFisher Scientific). For immunohistochemistry ...
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bioRxiv - Biophysics 2020Quote: ... followed by 1 h incubation with the appropriate Alexa-Fluor conjugated secondary antibodies (Thermo Fisher Scientific; 1:500). They were then stained for 5 min with 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Biochemistry 2020Quote: ... The secondary antibodies used were goat anti-mouse IgG (H+L) antibody conjugated to HRP (Invitrogen, catalog # 31430) and goat anti-rabbit IgG (H+L ...
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bioRxiv - Bioengineering 2021Quote: ... or Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 568 (Thermofisher, A-11031) were diluted 1:200 in PBS and incubated with the cells for 4 hours at 4°C in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... or Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 568 (Thermofisher, A-11031) were diluted 1:200 in PBS and incubated with the cells for 4 hours at 4°C in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... or Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 568 (Thermofisher, A-11031) were diluted 1:200 in PBS and incubated with the cells for 4 hours at 4°C in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... or Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor 568 (Thermofisher, A-11031) were diluted 1:200 in PBS and incubated with the cells for 4 hours at 4°C in the dark ...
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bioRxiv - Bioengineering 2021Quote: ... The proteins were detected with horse radish peroxidase (HRP)-conjugated rabbit anti-goat IgG (H+L) (R21459, Invitrogen), goat anti-mouse IgG ...
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bioRxiv - Bioengineering 2020Quote: ... immunolabeling was performed over a duration of 24 h using donkey-anti mouse Alexa Fluor 555/647 (Invitrogen), donkey-anti rabbit Alexa Fluor 555/647 (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... plating media was removed after 3 h and 900 μl maintenance media (Neurobasal media (Fisher Scientific # 21103-049) with 2 mM glutamine ...
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bioRxiv - Immunology 2020Quote: ... and mouse anti-human HLA-DR antibody (Thermo Fisher Scientific, USA, 1:100, 2 h at 37°C). Also ...
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Nanoscale molecular architecture controls calcium diffusion and ER replenishment in dendritic spinesbioRxiv - Neuroscience 2021Quote: Cultures were incubated for 1 h in Fluo-2 (high affinity) AM (2 μm; Invitrogen, Carlsbad, CA, USA) containing recording medium containing (in mM ...
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bioRxiv - Immunology 2021Quote: ... 1/200 concentrated Alexa Fluor™ 633-conjugated goat anti-mouse IgG (H+L) (Invitrogen, ThermoFisher Scientific, USA) and Cy3-conjugated goat anti-mouse IgG plus IgM (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Immunology 2021Quote: ... 1/200 concentrated Alexa Fluor™ 633-conjugated goat anti-mouse IgG (H+L) (Invitrogen, ThermoFisher Scientific, USA) and Cy3-conjugated goat anti-mouse IgG plus IgM (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were washed twice with PBS and were incubated for 48 h in DMEM (ThermoFisher Scientific, 31966-021) with or without 10 μg/ml 9EG7 antibody (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... and then with the following secondary antibodies: goat anti-human IgG (H+L) Alexafluor 488 (Invitrogen, 1/200), goat anti-mouse IgG2b Alexafluor 647 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... and goat anti-rabbit IgG (H+L) Alexa Fluor-568 (Thermo Fisher cat. No. A11036, 1:500 dilution). Cells were then washed with PBS with calcium and magnesium (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2021Quote: ... and incubated for 1 h at 4°C with 40 μL of Protein AG UltraLink Resin (Thermo Fisher). The beads were washed 2 × 5 min in ChIP Wash Buffer 1 (0.1% SDS ...
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bioRxiv - Plant Biology 2021Quote: ... cDNA was then prepared from RNA using Maxima H Minus First Strand cDNA Synthesis Kit (Thermo Scientific™) for RT-PCRs ...
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bioRxiv - Bioengineering 2021Quote: ... cells were grown in FreeStyle 293 expression medium and first co-transfected with H-chain- and L-chain-containing pcDNA3.1(+) plasmids using 293fectin according to the manufacturer’s protocol (Invitrogen). Stable transfectants producing either IgM617-HL or IgM012-HL were generated using cultivation in medium supplemented with 400 μg/ml G418 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Total RNA was extracted from the HEK293T cell line 24 h post HGF stimulation using TRIzol reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... for 1–2 h at 37°C followed by a 10 min incubation with Accutase (Thermo Fisher Scientific) at 37°C to obtain a single cell suspension ...
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bioRxiv - Neuroscience 2020Quote: ... at 42 °C with rotation in RT mix with 2000 U Maxima H Minus Reverse Transcriptase (ThermoFisher, MA) in 200 μl total volume ...
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bioRxiv - Plant Biology 2020Quote: ... and Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 488 (Invitrogen, A32731) were diluted by 1:400 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were incubated for 2 h with donkey anti-rabbit Alexa Fluor488 (1:200, ThermoFisher Scientific, A-21206) in 1% FBS PBS-Tx ...
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bioRxiv - Plant Biology 2021Quote: ... followed by a HRP-conjugated goat anti-mouse IgG (H+L) Secondary Antibody (G-21040, Thermo Fisher Scientific) in soybean ...
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bioRxiv - Plant Biology 2021Quote: ... for 1 h with gentle rotation at 4°C and then protein-G coated magnetic beads (Thermo Fisher) were added and incubated for an additional 30 min ...
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bioRxiv - Microbiology 2021Quote: ... and biofilms were mounted in VectaShield vibrance antifade mounting solution (Fisher Scientific, Pittsburgh, PA, catalog # H-1700-10) for a minimum of 1 hour at 4°C prior to imaging ...
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bioRxiv - Cell Biology 2020Quote: ... cells were washed and incubated for 1 h at RT with the secondary antibody (Alexafluor 488/568, Invitrogen). Images were captured either with a confocal laser scanning microscope (CLSM ...
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bioRxiv - Microbiology 2021Quote: ... Cells were washed and incubated for 2 h with Alexa 488-conjugated goat anti-mouse IgG antibody (Invitrogen) and with Alexa 546-conjugated goat anti-rabbit IgG antibody (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor 647 (1:500, Molecular Probes A21244), goat anti-mouse IgG (H+L ...
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bioRxiv - Microbiology 2020Quote: ... 0.1% Tween-20 and followed by 1 h incubation with Alexa fluorophore 488 secondary antibodies (Invitrogen, 1:400). The cells were finally washed three times with PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... Alexa Fluor™ 594 Donkey anti-Rabbit IgG (H+L) (Cat no. A-21207, 1:500, ThermoFisher Scientific) and Alexa Fluor™ 594 goat anti-mouse IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... and Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 647 (Invitrogen, A32795) diluted 1:750 in antibody blocking solution ...
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bioRxiv - Neuroscience 2020Quote: ... and Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody Alexa Fluor Plus 647 (Invitrogen, A32795) diluted 1:750 in antibody blocking solution ...
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bioRxiv - Cell Biology 2021Quote: ... Then 5.7 μl of a Reverse Transcription mix containing 3.5 U/μl of Maxima H minus retrotranscriptase (ThermoFisher), 0.88 U/μl of Superase-In RNase Inhibitor ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 U/μl in 1X RevertAid H minus Buffer supplemented with 2 U/μl RNAse OUT (Thermo Scientific), 1 mM dNTPs (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... Four micrograms of RNA were reverse-transcribed using random nonamers and Maxima H Minus Reverse Transcriptase (Thermo Fisher). The cDNA product was isolated by alkaline hydrolysis and treated with RNase A.
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bioRxiv - Biochemistry 2022Quote: ... and/or Alexa Fluor 594® F(ab’)2 fragment of goat anti-mouse IgG (H+L) (Invitrogen). Samples were then washed with PBS 1X+1% BSA and resuspended in 200 μl of the same buffer ...
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bioRxiv - Genomics 2022Quote: ... The TdT reation was prepared using 4 μL of the resulting elutant and 5 μL of ice-cold TdT mix (standard Quartz-seq2 condition: 1× Thermopol buffer [New England Biolabs], 0.24 mM dATP [Invitrogen], 0.0384 U/μL RNase H [Invitrogen] ...
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bioRxiv - Developmental Biology 2022Quote: ... Primary antibodies were detected with goat anti-rabbit IgG (H+L) Alexa Fluor Plus 488 rabbit (Thermo Fisher Scientific Cat# A32731TR ...
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bioRxiv - Bioengineering 2022Quote: ... and dialyzed against UPW for 36 h using SnakeSkin Dialysis Tubing (11532541, Thermo Fisher Scientific, Breda, The Netherlands). After dialysis ...
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bioRxiv - Molecular Biology 2022Quote: ... was used for reverse transcription and the resulting cDNA was treated with 1.25 U/μl RNase H (Invitrogen). cDNAs were amplified by 15 PCR using GoTaq G2 polymerase (Promega ...
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bioRxiv - Molecular Biology 2022Quote: ... We ligated both with an insert:vector ratio of 1:4 using Ligase (Thermo Scientific; 1 h, 22 C). The ligation mix was purified (Zymo Research ...
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bioRxiv - Bioengineering 2022Quote: ... and incubated for 1 h with Alexa Fluor 488-labelled goat anti-rabbit secondary antibody (Thermo Fisher Scientific) diluted 1:500 in PBS containing 0.2% saponin and 10% normal goat serum ...
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bioRxiv - Neuroscience 2020Quote: ... and cross-adsorbed secondary antibodies to IgG (H+L): goat Alexa Fluor 488 anti-rabbit (1:800; Invitrogen) and goat Alexa Fluor 568 (1:400 ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by growth in the absence of IL-2 for 6 - 9 h in RPMI 1640 (Gibco, # 21875034) medium supplemented with 10% FBS (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... First strand cDNA was generated using a Maxima H minus First Strand cDNA Synthesis Kit (Thermo Scientific, K1652) with the addition of 1μg of Actinomycin D (Sigma ...