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Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... RNA (2 μg) was reverse transcribed using a high-capacity cDNA reverse transcription kit (Applied Biosystems). Primers (5’ to 3’ ...
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bioRxiv - Genomics 2020Quote: ... 2 μg of RNA was used for reverse transcription (High Capacity cDNA Reverse Transcription Kit, ThermoFisher). Quantitative PCR detecting Gfi1b and Actb was carried out using SYBR green master mix (Bio-Rad) ...
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bioRxiv - Bioengineering 2019Quote: ... 2 µg of RNA were reverse transcribed using High-Capacity RNA-to-cDNA Kit (Applied Biosystems) following to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2020Quote: ... injection of EdU (5-ethynyl-2’-deoxyuridine; Click-it EdU Alexa Fluor 488 imaging kit, Invitrogen) at 5 and 6 days DPD at 50 mg/kg ...
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bioRxiv - Pathology 2022Quote: Total RNA was extracted from the C3H/10T1/2 cells using Trizol RNA extraction kit (Invitrogen). A total of 1 µg of extracted RNA was transcribed into cDNA using GoScript Reverse Transcription Kit (Promega) ...
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bioRxiv - Plant Biology 2022Quote: ... 2 μg tNA were DNaseI digested with DNA-free kit DNase Treatment and Removal Reagents (Ambion) following the manufacturers’“rigorous” DNase treatment protocol ...
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bioRxiv - Genetics 2019Quote: ... 2 μg RNA were reverse transcribed using the SuperScript IV Reverse Transcriptase kit (ThermoFisher Scientific, 18091200)) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2019Quote: ... 2 μg RNA was reverse transcribed using a high capacity cDNA reverse transcription kit (Applied Biosystems). Expression levels were normalised against PEX4 (At5G25760 ...
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bioRxiv - Immunology 2019Quote: ... The IFN-α/IFN-β 2-Plex Mouse ProcartaPlexTM Luminex Panel kit used was from Invitrogen. Tetramethylrhodamine ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 2 μl per sample was used in 10 μl sequencing reactions (BigDye Sequencing Kit, Applied Biosystems). Samples were sequenced in one direction for each gene using an automated 3130xl DNA Analyzer (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... kit and single-stranded cDNA was generated from 1-2 μg RNA using Superscript III (Invitrogen) and Oligo dT primers following the manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... extracted RNA was treated with 2 U DNase (TURBO DNA-free kit, Ambion, Foster City, USA). The concentration of total RNA was measured via Qubit Fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: A commercially available SARS-CoV-2 Inhibitor Screening Kit (Cat#: 16605302, Fisher Scientific GmbH, Schwerte, Germany) was used for cell free detection of SARS-CoV-2 Spike – ACE2 interaction inhibition ...
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bioRxiv - Genomics 2021Quote: ... DNase treatment was done on 2 μg of total RNA with DNA free DNase Kit (Invitrogen). From 1 μg of total RNA ...
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bioRxiv - Cell Biology 2022Quote: Kinase activity of Src variants was measured using Omnia® Y Peptide 2 Kit (ThermoFisher Scientific). The assay is based on the detection of fluorescence increase after kinase-specific substrate phosphorylation ...
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bioRxiv - Genetics 2024Quote: ... and 2 µg RNA was used for cDNA synthesis (Thermo Scientific, Verso cDNA synthesis kit, AB1453A). qPCR was performed using SYBR Green master mix (Bio-Rad Laboratories) ...
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bioRxiv - Developmental Biology 2023Quote: The medusae were incubated with 150 μM 5-ethynyl-2’-deoxyuridine (EdU) (EdU kit; Invitrogen, C10337) in ASW for 1 h or 24 h ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µg RNA were reverse transcribed using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific). Quantitative PCR was performed using the SensiFAST SYBR No-ROX kit (Bioline ...
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bioRxiv - Microbiology 2023Quote: ... 2 hours) and quantified using the 1X dsDNA HS kit with the Qubit 4.0 Fluorometer (Invitrogen). Amplicons were sequenced on a MiSeq using the 150-cycle v3 reagent kit (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... 2 hours) and quantified using the 1X dsDNA HS kit with the Qubit 4.0 Fluorometer (Invitrogen). Amplicons were sequenced on Illumina MiSeq and NovaSeq platforms in single-end format (150 bp ...
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bioRxiv - Biochemistry 2023Quote: ... 2 μg of template DNA was incubated and transcribed using Megascript T7 kit (Thermo Fisher Scientific). When RNA was synthesized ...
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bioRxiv - Molecular Biology 2020Quote: ... containing 2 μM Fura-2 acetoxymethyl ester (Fura-2 AM; ThermoFisher Scientific) and 0.01% pluronic acid (Merck ...
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bioRxiv - Immunology 2020Quote: ... The peptide was conjugated directly to surface lysines on Qβ bacteriophage VLPs using the bidirectional crosslinker succinimidyl 6-[(beta-maleimidopropionamido) hexanoate] (SMPH; Thermo Fisher Scientific) as previously described 47 ...
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bioRxiv - Microbiology 2021Quote: ... The mutations for Alpha and Beta (Fig. 4A) were added in silico to the codon-optimized Wuhan strain and ordered as synthetic genes (GeneArt, Thermo Fisher Scientific) and cloned into the same backbone (Planas et al. ...
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bioRxiv - Cell Biology 2019Quote: ... In immunofluorescence and western blot study we used the following antibodies: mouse Beta Tubulin loading control (BT7R) (Thermo fisher, cat no MA516308), mouse monoclonal CDK5 antibody (invitrogen ...
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bioRxiv - Neuroscience 2021Quote: The cells were lysed directly in the wells by addition of 300 μl Buffer RLT supplemented with 15 mM beta-mercaptoethanol (Thermo Fisher Scientific) after a wash with Dulbecco’s Phosphate-Buffered Saline (DPBS ...
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bioRxiv - Neuroscience 2021Quote: The cells were lysed directly in the wells by addition of 300 μl Buffer RLT supplemented with 15 mM beta-mercaptoethanol (Thermo Fisher Scientific) after a wash with Dulbecco’s Phosphate-Buffered Saline (DPBS ...
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bioRxiv - Immunology 2021Quote: ... and was conjugated directly to surface lysines on Qβ bacteriophage VLPs using the bidirectional crosslinker succinimidyl 6-[(beta-maleimidopropionamido) hexanoate] (SMPH; Thermo Fisher Scientific) as previously described 10 ...
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bioRxiv - Molecular Biology 2020Quote: ... Rabbit antibody to pSMAD 1/5/8 (1:100; CST 9516) and mouse antibody to beta-amyloid (1:100; Invitrogen 13-200) were diluted in the same 3% blocking buffer and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... auxiliary subunits facilitated by piggyBac transposon mediated genome insertion (13) The resulting cell line (HEK-beta cells) were maintained in Dulbecco’s modified Eagle’s medium (GIBCO/Invitrogen, San Diego, CA, USA) supplemented with 10% fetal bovine serum (Atlanta Biologicals ...
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bioRxiv - Developmental Biology 2022Quote: ... mPSCs were cultured using N2B27-based medium with addition of LIF and inhibitors for GSK3-beta and ERK (NB2iL) comprised of 48% Neurobasal Medium (Gibco, Gaithersburg, MD), 48% Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Cell Biology 2023Quote: Supernatants of cell culture medium collected during medium exchanges were analysed for albumin (10242, Diagnostic Systems) and 3-beta-hydroxybutyrate (Autokit 3-HB, Fujifilm Wako) on an Indiko Plus chemical analyzer (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... 100 μM isopropyl-β-D-thiogalactopyranoside (IPTG) and 100 μg/mL 5-bromo-4-chloro-3-indolyl-beta-D-galacto-pyranoside (X-gal) (Thermo Fisher Scientific). After incubation overnight at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... + 100 mM beta-mercaptoethanol at 80 °C for 10 min and run on 3-8% Tris-acetate gels (Thermo Fisher Scientific, EA0375PK2). Gels were run at 80V for 3 h and transferred onto a PVDF membrane in Tris-Glycine buler for 90 min at 90V ...
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bioRxiv - Systems Biology 2024Quote: Xenopus immunostaining was carried out as previously described102 with a primary antibody against beta-tubulin (1:100, DSHB E7) and a secondary goat anti-mouse fluorescent antibody (1:250, Life Technologies A32723).
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 488 chicken anti-rat IgG (Invitrogen Molecular probes Cat#A21470); and Alexa Fluor 594 donkey anti-rat IgG (Invitrogen Molecular probes Cat#A21209).
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 488 chicken anti-rat IgG (Invitrogen Molecular probes Cat#A21470); and Alexa Fluor 594 donkey anti-rat IgG (Invitrogen Molecular probes Cat#A21209).
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bioRxiv - Cell Biology 2020Quote: ... primary antibodies were visualized with Alexa 568–conjugated anti-rat antibodies (Invitrogen). Pictures were taken under 50x magnification ...
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bioRxiv - Cell Biology 2019Quote: ... E-cadherin was visualized with rat anti-E-cadherin (Invitrogen #13-1900), both at 1:100 concentrations as per manufacturer protocol ...
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bioRxiv - Immunology 2021Quote: ... antibodies diluted in PBS 0,1%Tween20 followed by α-rat 647 (Invitrogen) and α-rabbit AF488 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... goat anti-chicken IgY and goat anti-rat IgG secondary antibodies (Invitrogen) were used ...
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bioRxiv - Neuroscience 2022Quote: ... sections were stained with Alexa Fluor 647 goat anti-rat IgG (Invitrogen) for 2 hours ...
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bioRxiv - Neuroscience 2021Quote: ... and goat anti-rat Alexa Fluor 488 (1:500; Molecular Probes (Invitrogen) Cat# A-11006 ...
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bioRxiv - Neuroscience 2021Quote: ... and goat anti-rat Alexa Fluor 488 (1:500; Molecular Probes (Invitrogen) Cat# A-11006 ...
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bioRxiv - Molecular Biology 2020Quote: ... and goat anti-rat lgG Alexa Fluor 633 (each 1:1000, Invitrogen). Samples were incubated in secondary antibodies for 1 day at 4°C with gentle rotation ...
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bioRxiv - Cell Biology 2022Quote: ... rat monoclonal anti-LYVE1 (1:1000, Thermo Fisher Scientific, 14-0443-82), rabbit polyclonal anti-LYVE1 (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... and rat anti-mCherry antibody (M11217, Molecular probes, Thermo Scientific, 1:300). Secondary antibodies used are as following ...
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bioRxiv - Developmental Biology 2022Quote: ... and rat anti-mCherry antibody (M11217, Molecular probes, Thermo Scientific, 1:300). Secondary antibodies used are as following ...
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bioRxiv - Developmental Biology 2019Quote: ... goat-anti rat Alexa Fluor 555 (Molecular Probes, A-21434, 1:1000) and goat anti-rabbit Alexa Fluor 488 (Molecular Probes ...
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bioRxiv - Cell Biology 2020Quote: ... Dynabeads sheep anti-rat IgG was purchased from Invitrogen (Carlsbad, CA, USA). VectaMount permanent mounting medium was used from Vector Laboratories (Burlingame ...