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Citations for Thermo Fisher :
3201 - 3250 of 10000+ citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... or Insulin Receptor beta (ab69508) overnight at 4°C followed by anti-rabbit secondary antibody conjugated to horseradish peroxidase (ThermoFisher Scientific). Proteins were detected by Luminata Crescendo immunoblot HRP substrate (WBLUR0500Millipore Corporation ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The assay procedure is based on the manufacturer’s protocol for LanthaScreen™ TR-FRET Thyroid Receptor beta Coactivator Assay (Invitrogen, PV4686) with slight ...
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bioRxiv - Cell Biology 2020Quote: ... reverse: 5’-GGGGTCGGGAGGAACGG-3’ (Macrogen, South Korea) and beta-actin forward: 5’-CCTGGTCGGTTTGATGTT-3’ and reverse: 5’-GTGCGACGAAGACGA-3’ (Invitrogen, USA). Data analysis was made in CFX ManagerTM Software (BioRad ...
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bioRxiv - Molecular Biology 2024Quote: ... the Anti-beta actin antibody was reprobed after stripping the membranes using Restore™ stripping buffer (Thermo Fisher Scientific, Cat# 21059).
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bioRxiv - Cell Biology 2024Quote: The fluorescent cholesterol probe was tracked by incubating the cells with a final concentration of 20 μg/ml of TopFluor® Cholesterol (810255, Avanti) and 200 μg/ml of methyl-beta- cyclodextrin (C4555-1G, ThermoFisher) in PBS for 2 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with the following antibodies: mouse anti-Beta-actin (1:1000, Thermo Fisher Scientific, AM4302) and rabbit anti-PROX1 (1:1000 ...
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bioRxiv - Biochemistry 2023Quote: ... Dried lysed cellular lysate was digested using a solution of 5 nanogram/microliter LCMS grade trypsin (Pierce) in 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) and 50mM TEAB ...
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bioRxiv - Biochemistry 2023Quote: ... Dried cell lysate was digested using a solution of 5 nanogram/microliter LCMS grade trypsin (Pierce) in 0.1% n-Dodecyl-beta-Maltoside Detergent (DDM, Thermo Fisher, 89902) and 50mM TEAB ...
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bioRxiv - Microbiology 2021Quote: ... Lung supernatant and serum cytokines were measured using a ProcartaPlex Multiplex Immunoassay kit: Th Complete 14-Plex Rat ProcartaPlex Panel (Thermo Fisher Scientific Cat. EPX140-30120-901) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... 0.5% 2-mercaptoethanol (2-ME) (GIBCO) and 1% HEPES (GIBCO) ...
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bioRxiv - Genomics 2022Quote: PCR amplicons were purified using the QIAGEN QIAquick PCR Purification kit (Catalog No. 28104) and cloned using the TOPO™ XL-2 Complete PCR Cloning Kit (ThermoFisher Scientific, USA) with One Shot TOP10 Chemically Competent E ...
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bioRxiv - Cell Biology 2020Quote: ... The two genes were then transferred from the entry vector into the binary vector pB7HFC3_0 (11) using Gateway cloning (Life Technologies), to create the vectors p35S:YFP-FLAG and p35S:FLOE1-FLAG.
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bioRxiv - Immunology 2019Quote: ... adult females aged 6 to 11 days after eclosure were injected with fluorescent bioparticles (E. coli K-12 Strain Bioparticles TexasRed Conjugate; Invitrogen) or pH sensitive fluorescent bioparticles (pHrodo E ...
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bioRxiv - Immunology 2019Quote: ... adult females aged 3 or 11 days after eclosure were injected with a suspension of fluorescent beads (FluoSpheres carboxylate-modified 0.2 μm; Life Technologies); 50-69 nl of a 1:10 dilution in PBS of was injected as described previously (Makhijani et al. ...
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bioRxiv - Genetics 2019Quote: ... anti-CD127-FITC and (30 µl/ml, clone eBioRDR5, Cat. no.11-1278-42, Thermo Fisher Scientific, Waltham, U. S.) and anti-CD25-PE (80 µl/ml ...
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bioRxiv - Biophysics 2021Quote: ... The other end was ligated to a ∼190 bp biotin-labeled fragments created similarly by ApaI restriction of a 375 bp PCR amplicon of plasmid pBluKSP containing biotin-11-dUTP (Invitrogen, Life Technologies ...
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bioRxiv - Biophysics 2020Quote: ... Transmembrane protein constructs with extracellular HA-group were cross-linked with Biotin anti-HA.11 Tag Antibody (BioLegends) and Streptavidin Alexa Fluor 647 (Thermofisher).
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bioRxiv - Biochemistry 2021Quote: Proteins were identified by comparing the MS/MS data against Homo sapiens protein database (UniProt; 42054 entries, 2016-11-30) using Proteome Discoverer (v2.1, Thermo Scientific). Search parameters included MS1 mass tolerance of 10 ppm and fragment tolerance of 0.2 Da ...
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bioRxiv - Microbiology 2020Quote: ... The cDNA was then eluted off the beads in 11 μL of a ROX-formamide mixture (2.75 uL of ROX-500 ladder (Applied Biosystems) in 1.2 mL of HiDi-formamide (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... Both HEK293T and A549 cell lines were maintained at 37°C and 5% CO2 in high-glucose DMEM (11-965-092, Gibco), supplemented with 10% fetal bovine serum (Genclone ...
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bioRxiv - Microbiology 2021Quote: ... Calu-3 bronchial adenocarcinoma cells (ATCC ® HTB-55™) (11) were cultured in DMEM-F12 (1X) (Thermo-Fisher Scientific) supplemented with 10% FBS and 1% penicillin-streptomycin ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were stained (10 μL/1.5×105 cells) with anti-IL-1 alpha (Thermo Fisher Scientific; Cat.# 11-7118-81) and anti IgG1 kappa (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were fixed with 11 % glutaraldehyde and subsequently Hoechst-stained (10 mg/ml stock in 1× PBS, 1:2500; Invitrogen) using a Tecan Evo 200 robotic platform ...
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bioRxiv - Cell Biology 2021Quote: ... 60 picomoles siRNA and 11 μl of RNAiMAX were diluted separately in 250 μl of Opti-MEM (Gibco#11058-021). These diluted solutions were mixed together ...
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bioRxiv - Biophysics 2022Quote: Purified SN25 FL or 11–206 (E55W/R59C) was labeled with 10× molar excess monobrombimane (mBBr, Molecular Probes; Eugene, OR) in buffer 25 mM HEPES pH 7.4 ...
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bioRxiv - Cancer Biology 2022Quote: The transfection of siRNA into MOLM-14 and MV4-11 cells was performed using the Neon transfection system (Life Technologies) according to the manufacturer’s recommendations (1700V ...
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bioRxiv - Microbiology 2021Quote: Individual plasmids encoding 11 human genes or empty control vector were transfected individually into U-87 MG cells with Lipofectamine 3000 (Invitrogen) 48 hours prior to infection with VEEV-TC-83-nLuc.
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bioRxiv - Microbiology 2019Quote: ... The complete protocol can be found in Virus Host Interactions Methods and Protocols (30). Low passage NHDF cell suspension (approx. passage 11) were reverse-transfected with siRNA and Lipofectamine RNAiMAX (Invitrogen) using MultiDrop 384 ...
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bioRxiv - Immunology 2020Quote: ... Total RNA (1 μg) was reversed transcribed to cDNA using SuperScript™ VILO™ Master Mix (Invitrogen, 11-755-050).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Two assay media were employed for the analysis of parasites: RPMI 1640 medium ((Thermo Fisher Scientific, containing glucose 11 mM) and Seahorse XF RPMI Medium (Agilent Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... fresh human pancreatic islets preparations were resuspended in CMRL culture medium (cat. 11-530-037, ThermoFisher Scientific, Waltham, MA, USA) supplemented with L-Glutamine 1% (cat.G7513-100ML) ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Total RNA (1 μg) was reversed transcribed to cDNA using SuperScript™ VILO™ Master Mix (Invitrogen, 11-755-050) and RT-PCR performed on a CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Bioengineering 2021Quote: ... All plates were spread with 150uL of the yMM1456 (suc2Δ leu2Δ) strain with glass beads (Fisher Scientific, #11-312B 4mm), the beads were removed and the plate allowed to dry for 10 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... fresh human pancreatic islets preparations were resuspended in CMRL culture medium (cat. 11-530-037, Fisher Scientific, Pittsburgh, PA, USA) supplemented with L-Glutamine 1% (cat ...
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bioRxiv - Immunology 2022Quote: ... Proteins were detected on a Li-Cor Odyssey Blot Imager using an anti-Caspase-11 primary antibody (cone 17D9) and Alexfluor-680 conjugated secondary antibody (Invitrogen).
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bioRxiv - Systems Biology 2023Quote: ... then eluted by incubation for 20 min in 11 µL Formamide-ROX350 mix (1000 µL Hi-Di Formamide (Thermo Fisher) and 8 µL ROX350 ladder (Thermo Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... 20 three months old transgenic TgN3R182C150 mice were immunized initially either with 25 µg of aggregated NOTCH3 EGF1-5 protein (n=11, vaccinated) plus adjuvant (Imject Alum Adjuvant, ThermoFisher) or PBS (n=9 ...
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bioRxiv - Cell Biology 2022Quote: Utricles from vestibular schwannoma patients or organ donors were placed in DMEM/F12 (Thermo Fisher Scientific/Gibco, 11-039-021) with 5% FBS during transport to the laboratory ...
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bioRxiv - Cell Biology 2022Quote: Utricles from vestibular schwannoma patients or organ donors were placed in DMEM/F12 (Thermo Fisher Scientific/Gibco, 11-039-021) with 5% FBS during transport to the laboratory ...
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bioRxiv - Cancer Biology 2024Quote: ... were stained with 1µM (MOLM-13 and MV-4-11) or 3µM (HL-60) CellTrace Far Red (Thermo Fisher Scientific) to monitor cell divisions ...
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bioRxiv - Bioengineering 2024Quote: ... At day 11 a glucose starvation was carried out by replacing the medium with RPMI 1640 without glucose (Life Technologies), supplemented by B27 with insulin for 3 days ...
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bioRxiv - Cell Biology 2023Quote: ... The Isolated islets were hand-picked and cultured in complete media (RMPI-1640 containing 11 mM glucose, 10% FBS, 0.1% Penicillin/Streptomycin, gentamicin and Amphotericin B; Thermo Fisher) and maintained at 37°C in 5% CO2 environment to allow their recovery.
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bioRxiv - Neuroscience 2023Quote: C26 cells were cultured in complete growth medium consisting of RPMI 1640 medium with Glutamine (#11-875-093; Thermo Fisher) containing 10% of heat-inactivated Fetal Bovine Serum (FBS ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were plated 24 hours before transfection in a cell-culture treated 6-well plate (Fisher Scientific, 14-832-11). To transfect ...
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bioRxiv - Immunology 2023Quote: ... and resuspended in 150 µL of an antibody cocktail containing 1:200 anti-Ter119 (FITC; Thermo Scientific, 11-5921-82), 1:50 anti-CD31 (FITC ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA-mediated depletion of endogenous ESCO2 was done with 20nM siRNA (Dharmacon, J-025788-11, target nucleotides: GUUGAUACCCUCAGGAAUU) in serum-free Opti-MEM (Gibco) 24 hours after induction ...
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bioRxiv - Bioengineering 2023Quote: ... Purity of gDNA was assessed using either a Denovix DS-11 spectrophotometer or the Qubit 3 fluorometer (Thermo Fisher Scientific) and dsDNA Broad Range Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2024Quote: ... and monitored using a Traceable™ Jumbo Thermo-Humidity Meter (cat. #11-661-19, Fisher Scientific, Saint-Laurent, Quebec, Canada).
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bioRxiv - Microbiology 2024Quote: ... The cDNA was eluted in 11 µL of a ROX-formamide mixture composed of 2.75 µL of ROX-500 ladder (Applied Biosystems) in 1.2 mL of HiDi-formamide (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: ... Randomisation was removed and samples were mass tagged using 10 channels of a TMT-11-plex stock (Thermo Fisher Scientific). For quantification control ...