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Citations for Thermo Fisher :
3101 - 3150 of 5976 citations for M CSF Rat HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Whole-cell lysates were prepared using M-PER lysis buffer (Thermo Fisher, MA) and clarified by centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was transcribed to cDNA with M-MLV reverse transcriptase (Invitrogen, cat#28025013) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... and samples were by homogenizing using M-PERTM (Thermo Fisher Scientific, Cat#78501) Halt Protease Inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... purified CEN DNA was conjugated to Streptadivin-coated Dynabeads (M-280 Streptavidin, Invitrogen) for 2.5 hr at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... The biotinylated DNA fragments were purified using Dynalbeads M-280 streptavidin beads (Invitrogen) as outlined in the Illumina Mate-Pair protocol ...
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bioRxiv - Biochemistry 2023Quote: ... 0.25 M EDTA-Na (pH 8.0) and 0.5 mg/ml glycogen (Thermo Fisher). Following precipitation with 70% ethanol ...
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bioRxiv - Neuroscience 2023Quote: ... DRG were next embedded in Shandon M-1 Embedding Matrix (Thermo Fisher Scientific), snap frozen on dry ice and stored at −80□°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Reduced RNAs were precipitated with 3 M sodium acetate pH 5.2 (Thermo Fisher), glycogen (Nacalai Tesque ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µg of RNA was reverse transcribed with M-MuLV reverse transcriptase (Invitrogen) as described [14].
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bioRxiv - Immunology 2023Quote: ... 5x10-5 M 2-mercaptoethanol (2-ME) and 5 mM HEPES (all Invitrogen).
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bioRxiv - Immunology 2023Quote: ... cDNA synthesis was performed using M-MLV Reverse Transcriptase (28025013, Thermo Fisher Scientific) and qPCR was performed with the Gotaq qPCR master mix (A6001 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.8 M guanidine HCl and 6 units/mL Proteinase K (AM2546, Thermo Fisher). Gel fragments were digested at 4°C overnight ...
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bioRxiv - Bioengineering 2022Quote: ... and 83% 10 M HEPES buffer (Gibco™ through Thermo Fisher Scientific, Loughborough). The collagen/cell mix had final volumes of 80% collagen ...
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bioRxiv - Cell Biology 2022Quote: ΔΨm was measured using tetramethylrhodamine (TMRM) or Mito-Tracker Red (Invitrogen, Carlsbad, CA). For TMRM staining ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was prepared using the M-MLV Reverse Transcriptase (Invitrogen, Carlsbad, CA, USA) kit and random hexamers ...
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bioRxiv - Cancer Biology 2023Quote: ... and using Dynabeads™ M-280 Sheep Anti-Rabbit IgG (Invitrogen Cat# 11203D). Chromatin Immunoprecipated DNA was amplified by qPCR using a CFX Connect Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... 20 x 106 Dynabeads™ M-450 Tosyl-activated (#14013, Thermo Fisher Scientific) were washed two times with Buffer 1 (0.1M sodium phosphate buffer ...
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bioRxiv - Bioengineering 2023Quote: ... The biotinylated recombinant toxins were captured using streptavidin-coated Dynabeads (Invitrogen, M-280). After washing with PBS containing 0.1% (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (1 M) (Invitrogen, cat.#15630080), CellMask membrane dye (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... 2 M NaCl) and 0.5 uL SUPERase•In RNase Inhibitor (Thermo Fisher, AM2694) followed by a 10 minute incubation at 68°C and slow cool down to 37°C for hybridization ...
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bioRxiv - Microbiology 2023Quote: ... The cells pellets were resuspended in M-PER reagent (Thermo Scientific, Cat #78503) containing protease inhibitor (Thermo scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein lysates were prepared using M-PER mammalian extraction buffer (Pierce, Thermo Scientific) supplemented with 1× protease and phosphatase inhibitor cocktail ...
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bioRxiv - Plant Biology 2023Quote: ... Hybridized targets were recovered using Dynabeads M-280 Streptavidin (ThermoFisher Scientific, Vilnius, Lithuania) following the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... and subsequently bound to Dynabeads M-280 Streptavidin (Invitrogen Thermo Fisher Scientific, USA) at room temperature for 30 minutes.
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were enriched using magnetic streptavidin beads (Dynabeads M-280, Invitrogen, USA). The beads were washed with a series of SDS buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 × 10-4 M MTG and 5% Protein-Free Hybridoma Media II (Gibco).
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bioRxiv - Bioengineering 2023Quote: ... 1 µg RNA was reverse-transcribed using M-MLV Reverse Transcriptase Kit (Invitrogen). qRT-PCR was performed using SYBR Green (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatants were incubated with 30 μl of Dynabeads M-270 Streptavidin (Invitrogen), preequilibrated with lysis buffer containing 1% Triton X-100 ...
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bioRxiv - Bioengineering 2023Quote: ... and subsequently bound to Dynabeads M-280 Streptavidin (Invitrogen Thermo Fisher Scientific, USA) at room temperature for 30 minutes.
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bioRxiv - Bioengineering 2023Quote: ... To this mixture we added glycerol 2-phosphate (3 M, Thermo Fisher Scientific) and (GT)15-SWCNTs (3.75 mg/L ...
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bioRxiv - Microbiology 2023Quote: ... 0.8 µl DTT (0.5 M) 40 U SUPERase-InTM RNase Inhibitor (Ambion®) and 43.7 µl 2% Sarkosyl for run-ons with sarkosyl ...
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bioRxiv - Cancer Biology 2023Quote: ... 1000 ng total RNA is reverse transcribed using M-MLV Reverse Transcriptase (Invitrogen) cDNA synthesis kit following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was made using the M-MLV reverse transcriptase (Invitrogen, catalog number 28025013). qPCR was performed using Power up SYBR Green Master Mix (Thermo Fisher ...
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bioRxiv - Physiology 2024Quote: ... and cells were lysed in mammalian protein extraction reagent (M-PER; Thermo Fisher) containing protease inhibitors cocktail (Roche) ...
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bioRxiv - Bioengineering 2024Quote: ... and brought to a pH of 6.5 using 1 M NaOH (Fisher Scientific). To assemble the functionalized alginate gels ...
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bioRxiv - Neuroscience 2024Quote: ... brains were removed and frozen in embedding medium (M-1 Embedding Matrix, ThermoFisher) before storage at −80°C for at least 12h ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific), and Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... a 0.5 M solution (filter sterilized) of N-acetyl Cysteine (NAC) (Thermofisher, A15409.14) was used to add NAC at 5 mM to HEPES-NaOH (5 mM ...
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bioRxiv - Microbiology 2024Quote: ... a 100 µL of stop solution (0.16 M H2SO4, Thermo Fisher Scientific # N600) was added and the absorbance was recorded at 450 nm using a Biotek H1M monochromatic plate reader ...
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bioRxiv - Immunology 2021Quote: ... were transfected to HEK293 cells (ATCC® CRL-1573™) and each recombinant protein was purified using HisPur™ Ni-NTA column (Invitrogen, Cat# 88226). The specificity of recombinant protein was further validated by SDS-PAGE and Western blot evaluations.
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bioRxiv - Molecular Biology 2024Quote: Signaling via the intracellular Ca++ pathway was assessed in stably transfected HEK- HEK293/hPTH1R/glosensor (GP-2.3) cells using the calcium-sensitive fluorophore Fura2-AM (Invitrogen, Life Tech. Grand Island, NY, USA) [73 ...
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bioRxiv - Cancer Biology 2019Quote: ... equipped with a nanoACQUITY (Waters, Milford, MA, USA) ultraperformance LC (UPLC) system and an EASY-Spray PepMap C18 column (Thermo Fisher, ES800; 3-m bead size, 75 m by 150 mm) for reversed-phase chromatography ...
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bioRxiv - Cell Biology 2020Quote: ... and Alexa Fluor 594 donkey anti-rat IgG (Invitrogen Molecular probes Cat#A21209).
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bioRxiv - Cell Biology 2020Quote: ... and Alexa Fluor 594 donkey anti-rat IgG (Invitrogen Molecular probes Cat#A21209).
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bioRxiv - Cell Biology 2020Quote: ... followed by anti-Rabbit/Mouse/Rat Alexa-dye coupled antibodies (1:400, Invitrogen). LysoTracker FarRed was used to visualize lysosomes in live microscopy (1:2000 ...
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bioRxiv - Immunology 2021Quote: ... and incubated overnight at 4°C with rat anti-mouse CD8 (4SM15, Invitrogen) and rabbit anti-mouse Ly6G (E6Z1T ...
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bioRxiv - Developmental Biology 2021Quote: ... chicken anti-rat Alexa Fluor 647 (1:200, A-21472, Thermo Fisher Scientific), donkey anti-rabbit Alexa Fluor 647 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... donkey anti-rat Alexa Fluor 488 (1:200, A-21208, Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 555 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... donkey anti-rat Alexa Fluor 488 (1:200, A-21208, Thermo Fisher Scientific), donkey anti-goat Alexa Fluor 555 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... chicken anti-rat Alexa Fluor 647 (1:200, A-21472, Thermo Fisher Scientific)) and 4’,6-diamidino-2-phenylindole (DAPI ...