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Citations for Thermo Fisher :
3101 - 3150 of 5144 citations for HCCS Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Mass spectrometric analysis of eluting peptides was conducted on an Orbitrap Exploris 480 (Thermo Fisher Scientific; Waltham, US) instrument platform ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were reconstituted in 10 µl of H2O and reacted with 80 µg of TMT10plex (Thermo Scientific, #90111) label reagent dissolved in 4 µl of acetonitrile for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were loaded and concentrated on PepMap C18 precolumns (length, 5 mm; inner diameter, 0.3 mm; Thermo Scientific) at a flow rate of 30 µl/min and separated using Acclaim PepMap C18 reversed-phase nano-LC columns (length ...
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bioRxiv - Biochemistry 2022Quote: Lyophilized peptide samples were suspended in 0.25X PBS buffer and concentrations were determined using a Nanodrop 2000 (ThermoFisher). CD spectra were collected using a JASCO J-815 CD spectrometer sampling every 0.5 nM between 260 nM and 180 nM ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were separated on a 50 cm 75 μm inner diameter EasySpray ES903 microcapillary column (Thermo Fisher Scientific). Peptides were separated over a 190 min gradient of 6 - 27% acetonitrile in 1.0% formic acid with a flow rate of 300 nL/min.
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bioRxiv - Cancer Biology 2023Quote: ... of the mRNA solution was mixed with 5μl of 20mM p5RHH peptide solution and 187μl of 1x HBSS (Gibco) to prepare the nanoparticle complex and immediately injected into the mouse through the tail vein21 ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were collected by centrifugation and labelled using TMT 10-plex isobaric mass tagging reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... four micrograms of desalted peptides were labeled with tandem mass tags (TMT10plex, Thermo Fisher Scientific cat. No 90110) using a 1:20 ratio of peptides to TMT reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Analysis of the eluted tryptic peptides was performed online using a Q Exactive Plus mass spectrometer (Thermo Scientific) possessing a Nanospray Flex Ion source (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... The separated peptides were electrosprayed in the positive ion mode into an Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher) operated in data-dependent acquisition mode (3 s cycle time ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were diluted to 60 µL with LC/MS-grade water and labeled using TMTpro labels (Thermo Scientific) for 1 h at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Peptides were separated across a 2.1mm X 5cm C18 separating column (1.9 um Hypersil Gold, Thermo Fisher Scientific) with a linear gradient of 4% - 40% CH3CN and 0.3% formic acid ...
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bioRxiv - Neuroscience 2023Quote: ... 20 μg peptide from each sample was labeled with TMT10plex Isobaric Label Reagent Set (ThermoFisher Scientific, cat# A37725) following to the manufacture’ s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were trapped on a μPrecolumn C18 PepMap100 (5μm, 100 Å, 300 μm × 5mm, ThermoFisher Scientific, Reinach, Switzerland) and separated by backflush on a C18 column (5 μm ...
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bioRxiv - Immunology 2023Quote: ... PCSK9 peptides were conjugated to Qß bacteriophage VLPs using succinimidyl 6-((ß-maleimidopropionamido)hexanoate) (SMPH; Thermo Fisher Scientific), as described previously60 ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were separated on a 50 cm (2 µm particle size) EASY-Spray column (Thermo Fisher Scientific, UK), which was assembled on an EASY-Spray source (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: Peptides were fractionated using high-pH liquid-chromatography on a micro-flow HPLC (Dionex U3000 RSLC, Thermo Scientific). 45µg of pooled and purified TMT labelled peptides resuspended in Solvent A (5mM ammonium-bicarbonate ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were analysed by on-line nanoflow LC using the Ultimate 3000 nano system (Dionex/Thermo Fisher Scientific). Samples were loaded onto a trap column (Acclaim PepMap 100 ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were separated on an in-house 50 cm column (360 μm od, 75 μm id, Fisher Scientific) packed with ReproSil-Pur C18 (120 Å pore size ...
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bioRxiv - Plant Biology 2023Quote: Peptides were analyzed with 5 different MS methods on a Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific), summarized in Supplementary Dataset S7 ...
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bioRxiv - Developmental Biology 2023Quote: Raw mass spectrometry data was analyzed for peptide identification and quantification with Proteome Discoverer 2.5 (Thermo Fisher Scientific), using the SequestHT search engine against the Homo sapiens UniProt database (UP000005640 ...
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bioRxiv - Systems Biology 2023Quote: Tryptic peptides were separated by reverse phase chromatography on a Dionex Ultimate 3000 RSLCnano UHPLC system (Thermo Scientific) with an Acclaim C18 PepMap RSLC column using a 3-42% acetonitrile gradient over 60 minutes ...
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bioRxiv - Microbiology 2023Quote: Peptides were separated on a C18 nano-column (PepMap 100 C18 series. Thermo Fisher Scientific. Waltham, MA, USA) using an Ultimate 3000 nano-HPLC ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were trapped on a 2 cm nanoViper precolumn (i.d. 75 μm; C18, Acclaim PepMapTM 100, Thermo Scientific) with buffer A (2:98 MeCN:H2O in 0.1% formic acid ...
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bioRxiv - Cell Biology 2023Quote: ... peptide samples were desalted and subjected to analysis using an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific). For perturbation experiments (either coRIC or total proteome) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Aliquots of 30 µg peptides were labelled using TMTpro 18-plex isobaric mass tagging reagents (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting tryptic peptides in 10 μL solution was loaded onto a 2-cm trap column (Thermo Scientific) and separated on a 50-cm EASY-Spray analytical column (Thermo Scientific ...
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bioRxiv - Cell Biology 2023Quote: Desalted SILAC-labeled peptides were analyzed on a Fusion Lumos mass spectrometer (Thermo Fisher Scientific, San Jose, CA) equipped with a Thermo EASY-nLC 1200 LC system (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were separated by reverse-phase chromatography on an 25-cm Easy Spray column (Thermo Fisher Scientific, USA) connected to an EASY-nLC 1200 instrument (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... Peptides were loaded onto a trap column (PepMap 100 C18, 5 μm, 5 × 0.3 mm, Thermo Fisher Scientific) at a flow rate of 10 μL/min using 0.1% TFA as loading buffer ...
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bioRxiv - Microbiology 2023Quote: ... about 300 μg tryptic peptides were separated using a nanoflow DIONEX Ultimate 3000 RSLC nano System (ThermoFisher Scientific) with an XBridge Peptide BEH C18 column (300 Å ...
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bioRxiv - Microbiology 2023Quote: ... Peptide separation was carried out using an Acclaim PepMap trap column (75 μm × 2 cm; Thermo Fisher Scientific) and an EASY-Spray ES902 column (75 μm × 250 mm ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were trapped on a µPrecolumn C18 PepMap100 (5μm, 100 Å, 300 μm×5mm, ThermoFisher Scientific, Reinach, Switzerland) and separated by backflush on a C18 column (5 μm ...
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bioRxiv - Cell Biology 2023Quote: Peptides were separated by nanoscale C18 reverse-phase liquid chromatography using an EASY-nLC II 1200 (Thermo Scientific) coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were trapped on a 2 cm nanoviper Precolumn (i.d. 75 μm, C18 Acclaim PepMapTM 100, Thermo Scientific) at a flow rate of 3.0 µL/min in buffer A (2/98 MeCN/H2O in 0.1% formic acid ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were analyzed with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific, San Jose, CA, USA) in data-dependent mode ...
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bioRxiv - Developmental Biology 2024Quote: ... On-line peptide separation was performed on a 15-cm EASY-Spray™ C18 column (ES75150PN, Thermo Fisher) by applying a linear gradient of increasing ACN concentration at a flowrate of 200 nL/min ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were separated using an Ultimate 3000 nano rapid separation liquid chromatography system (Dionex, ThermoFisher Scientific, MA, USA) equipped with a trapping cartridge (Precolumn C18 PepMap100 ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting peptides were extracted from gel fragments and analyzed using Orbitrap Fusion Lumos mass spectrometer (Thermo Scientific) combined with UltiMate 3000 RSLC nano-flow HPLC (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 µl of the reconstituted peptides were separated on an EASY-nLC™ 1200 chromatography system (Thermo Scientific) coupled to a Q Exactive HF-X Orbitrap LC-MS/MS System (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: The purified peptides were analyzed using the reverse phase liquid chromatography system Easy-nLC 1200 UHPLC (Thermo Scientific) coupled to an Orbitrap Fusion Lumos Tribrid Mass Spectrometer (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were separated on a 25-cm PepMap C18 column packed with 2-μm particles (Thermo Fisher Scientific) using the mobile buffer consisted of 0.1% FA in ultra-pure water with an eluting buffer of 0.1% FA in 100% ACN with a 60 min linear gradient of 5 to 25% ACN/0.1% FA at a flow rate of 300 nl/min.
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bioRxiv - Physiology 2024Quote: ... Peptides (360 ng) were loaded (Acclaim PepMap100 C18 3µm beads with 100 Å pore-size, Thermo Fisher Scientific) and separated (1.9-µm particle size C18 ...
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bioRxiv - Plant Biology 2024Quote: ... Peptides were separated on a 25-cm PepMap C18 column packed with 2-μm particles (Thermo Fisher Scientific) using the mobile buffer consisted of 0.1% FA in ultra-pure water with an eluting buffer of 0.1% FA in 100% ACN with a 90 min linear gradient of 11 to 37% ACN/0.1% FA at a flow rate of 300 nl/min.
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were gradient eluted with a flowrate of 200nL/min into an Orbitrap Eclipse Tribrid (Thermo Fisher Scientific) mass spectrometer ...
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bioRxiv - Neuroscience 2024Quote: ... 700 fmol of standard synthetic AQUA peptides per mg of protein amount (Technologies, Thermo Fisher Scientific, Waltham, USA) were added to the endogenous peptide mixture and peptides were dried in a centrifugal Savant SpeedVac (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... After purification using SepPak C18 columns (Waters; WAT054955) the peptides were TMT-labeled (Thermo Fisher Scientific; 90061;TH266884) and fractionated with the High pH Reversed-phase Fractionation Kit (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptide extraction was performed using the EasyPep™ MS Sample Prep Kits (Thermo Fisher Scientific Inc, Massachusetts, USA), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... The peptides were analyzed by an Orbitrap Fusion Lumos tribrid mass spectrometer with an ESI source (ThermoFisher Scientific) coupled online to the nano-LC and operated in positive polarity ...
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bioRxiv - Cancer Biology 2024Quote: ... suspended in Formic Acid (FA) 0.1% and dosed with the quantitative fluorometric peptide assay (ref. 23290, Thermo Scientific).