Labshake search
Citations for Thermo Fisher :
3301 - 3350 of 10000+ citations for Estriol ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... The chemiluminescent Western blot detection kit (SuperSignal West Femto Chemiluminescent Substrate) and ATP determination kit were from Thermo Scientific (Rockford, IL, USA). The ELISA Kit for rat/mouse insulin ...
-
bioRxiv - Genomics 2020Quote: ... The oligonucleotides were subsequently transcribed in vitro using the manufacturer’s instructions (TranscriptAid T7 high yield transcription kit / MEGAscript SP6 transcription kit, both ThermoFisher Scientific, Waltham, USA). The gRNAs were purified ...
-
bioRxiv - Immunology 2021Quote: ... The remaining cell suspension was used to isolate CD8+ T cells with the magnetic-bead-based positive selection kit: Dynabead’s positive CD8+ isolation kit (Thermo Fisher, Waltham, MA). It is important to use an isolation kit that allows separation of cells from the beads for the samples to be compatible with 10x’s single-cell preparation ...
-
bioRxiv - Microbiology 2019Quote: ... Purified DNA and RNA from these four time points were quantified using Qubit dsDNA HS Assay Kit and Qubit RNA HS Assay Kit (Invitrogen, Carlsbad, CA), and also quantified using NEB Luna Universal qPCR Master Mix and Luna Universal One-Step RT-qPCR Kit (New England Biolabs ...
-
bioRxiv - Cancer Biology 2019Quote: ... RNA used was using QIAGEN® RNeasy Mini Kit and cDNA synthesised utilising the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems). 10ng of cDNA was used with the designed primers (Supplementary Table S4 ...
-
bioRxiv - Plant Biology 2020Quote: ... the fragments were cloned into entry vector pDONR207 and yeast expression vector pYesdest52 using Gateway BP Clonase II Enzyme Kit and LR Clonase II Enzyme Kit (Invitrogen, MA, USA), respectively.
-
bioRxiv - Genetics 2019Quote: ... The linearized Cas9 plasmids were transcribed into mRNA using the T7 m MESSAGE Kit (Ambition, USA) and gRNA was synthesized using transcript Aid T7 High Yield Transcription Kit (Thermo Scientific, USA). Zebrafish embryos at the one-cell stage were co-injected with 20pg target gRNA and 300pg Cas9 mRNA ...
-
bioRxiv - Developmental Biology 2020Quote: ... using the Agilent RNA 6000 Nano Kit and concentrations were assayed with the Qubit RNA Broad Range Assay Kit (Thermo Fisher Scientific). For the transcriptome analysis ...
-
bioRxiv - Neuroscience 2019Quote: ... sgRNA molecules from a PCR-amplifìed template were obtained by in vitro transcription (MAXIscript T7 Transcription Kit followed by MEGAclear Transcription Clean-Up Kit - Thermo Fisher Scientific). The template was produced from overlapping long primers (IDTDNA.com ...
-
bioRxiv - Microbiology 2019Quote: The DNA concentrations were measured fluorometrically (Qubit® 3.0, kit Qubit® dsDNA Broad Range Assay Kit, Life Technologies, Carlsbad, CA, USA). DNA integrity was determined by agarose gel electrophoresis using a 0.8% (w/v ...
-
bioRxiv - Genomics 2020Quote: Total RNA was extracted from whole blood using a PAXgene Blood RNA Kit version 2 (PreAnalytiX, #762164) and cDNA was generated with a High Capacity Reverse Transcription Kit (Applied Biosystems, #4368814) in an identical fashion as described for CDKL5 cDNA amplicon sequencing ...
-
bioRxiv - Immunology 2021Quote: ... The resulting libraries were profiled with a High Sensitivity NGS Fragment Analysis Kit (Advanced Analytical, Cat. #DNF-474) and measured with the Qubit dsDNA HS Assay Kit (Invitrogen, Cat. #Q32851) prior to pooling and sequencing on an Illumina NextSeq 500 sequencer utilizing a BRB-seq custom primer and the High Output v2 kit (75 cycles ...
-
bioRxiv - Plant Biology 2021Quote: ... 21 nt GAPDH-dsRNA (provided in the kit) was labeled with Cy™ 3 utilizing the Silencer™ siRNA Labeling kit (ThermoFisher) according to the manufacturer’s instructions.
-
bioRxiv - Plant Biology 2020Quote: Edu (5-ethynyl-2′-deoxyuridine) staining was performed using Click-iT® EdU AlexaFluor® 647 Imaging Kit as per the kit protocol (Invitrogen). The 5 DAG Col-0 and flz8 seedlings grown on 0.5X MS medium with 30 mM Suc were fixed in the fixation reagent (1X PBS with 3.7% formaldehyde and 0.5% Triton-X ...
-
bioRxiv - Microbiology 2020Quote: ... Total nucleic acids were purified from 500 µL of sample thawed on ice using the PowerMicrobiome RNA isolation kit (MO BIO Laboratories) with the optional phenol-based lysis or with the RiboPure RNA purification kit for bacteria (Thermo Fisher Scientific). Purification was carried out according to the manufacturers’ recommendations ...
-
bioRxiv - Microbiology 2020Quote: ... The resulting PCR product was purified using the QIAquick Gel Extraction Kit and then cloned with the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) into competent E ...
-
bioRxiv - Immunology 2021Quote: ... Secondary anti-rabbit Alexa Fluor 488 and T cell isolation kit (Dynabeads™ Untouched™ Mouse T Cells Kit, Cat. no. 11413D) were procured from Invitrogen, Carlsbad ...
-
bioRxiv - Immunology 2022Quote: Total RNA was harvested from 48-hour ex vivo B cell cultures using Quick-RNA™ Microprep Kit (ZymoResearch) and reverse transcribed to cDNA using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). TaqMan™ probes specific for Gen1 (Mm00724023_m1) ...
-
bioRxiv - Cancer Biology 2022Quote: RNA was extracted using the RNeasy Mini Kit (QIA-GEN) and cDNA was produced using the High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher Scientific). The quantitative polymerase chain reaction was performed using the Power SYBR Green PCR Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2022Quote: ... The Click-it reaction cocktail was prepared as described in the kit instructions (Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa F uor™ 647 dye, #C10340, Invitrogen), but with half the concentration of Alexa-647 dye ...
-
bioRxiv - Cell Biology 2021Quote: Total RNA from MG stimulated MCECs was extracted using peqGOLD MicroSpin Total RNA kit (Peqlab) and then converted into cDNA with a high capacity cDNA reverse transcription kit (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... DNA was quantified with the high sensitivity double stranded (ds) DNA Assay Kit (Qubit dsDNA HS Assay Kit, Thermo Fisher Scientific, #Q32854) on the Qubit 2.0 fluorometer.
-
bioRxiv - Cell Biology 2022Quote: ... templated and sequenced using either Ion 550™ Kit-Chef and Ion 550 Chip Kit as one 16-plex library pool or Ion 540™ Kit-Chef and Ion 540 Chip Kit as one 8-plex library pool (Thermo Fisher). Two independent sequencing analyses were performed on a panel of eight samples ...
-
bioRxiv - Molecular Biology 2019Quote: ... PCR amplicons of these four primer pairs were then purified from 1% agarose gel using Qiagen Gel Extraction kit and concentrations were measured with Qbit High Sensitivity DNA kit (Thermo Fisher Scientific). Each amplicon was then diluted to 1×109 molecules per µl ...
-
bioRxiv - Immunology 2019Quote: ... SHIV RNA was visualized with the 1-Plex ViewRNATM ISH Tissue Assay Kit using SIVmac239 or Beta actin (positive control) probe sets and ViewRNA Chromogenic Signal Amplification Kit (ThermoFisher, Waltham, MA). These two sequential slides were indivudually imaged with a Zeiss AxioObserver microscope and AxioCam MRm camera ...
-
bioRxiv - Microbiology 2019Quote: ... 0.1 ml of MTT solution (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) was added to the wells following the recommendation of the commercial kit (Vybrant® MTT Cell proliferation assay kit, ThermoFisher Scientific). After 2h of incubation at 37°C ...
-
bioRxiv - Pathology 2019Quote: Total RNAs were obtained from whole kidneys or cells using NucleoSpin® RNA Kit (Macherey Nagel) and reverse transcribed using High-Capacity cDNA Reverse Transcription kit (ThermoFisher Scientific). Quantitative PCR was performed with iTaq™ Universal SYBR® Green Supermix (Bio-Rad ...
-
bioRxiv - Cancer Biology 2019Quote: ... total pancreas RNA was isolated using a Qiagen RNeasy Plus Isolation Kit followed by cDNA synthesis (High Capacity cDNA RT kit, Applied Biosystems, 4374966). RT-PCR was performed using TaqMan Universal Master Mix II (Fisher ...
-
bioRxiv - Microbiology 2020Quote: ... The indexed amplicons were purified using the AMPure XP kit according to the manufacturer’s instructions followed by library quantification using a Qubit™ dsDNA BR Assay Kit (Thermo Fisher Scientific). All samples were normalized to 9 nM and pooled with 5 μl of each sample.
-
bioRxiv - Pathology 2020Quote: ... The pellet was rinsed twice in DDW and then lysis buffer from the Prepfiler BTA kit (PrepFiler® Express BTA Forensic Extraction Kit (Applied Biosystems) was added together with DTT (Sigma ...
-
bioRxiv - Molecular Biology 2020Quote: The transcription start site of AhLEC1A gene was identified by 5’ RACE (rapid amplification of cDNA ends) using a 5’ RACE kit (Invitrogen GeneRacerTM Kit) following the instructions provided by the manufacturer ...
-
bioRxiv - Genetics 2020Quote: ... Barcoded products were pooled and gel purified from a 1% agarose gel using a QIAgen kit (#28115) to remove primers before quantitation with a Qubit dsDNA HS Kit (ThermoFisher Scientific #Q32851). Samples were loaded onto an Illumina MiSeq instrument with a 300 cycle v2 kit for sequencing ...
-
bioRxiv - Microbiology 2020Quote: ... The PCR product was purified using the QIAquick Gel Extraction Kit after electrophoresis in agarose gel 1% and then cloned with the Zero Blunt® TOPO® PCR Cloning Kit (Invitrogen) into competent E ...
-
bioRxiv - Immunology 2021Quote: cDNA was reverse-transcribed from template RNA either using a two-step reverse transcription using AppScript cDNA synthesis kit (Appleton Woods) or using the High Capacity RNA-to-cDNA kit (Applied Biosystems, USA).
-
bioRxiv - Genetics 2020Quote: ... genomic DNA was extracted using the Qiagen DNeasy Blood & Tissue Kit (Cat.D No. 69504) and quantified using the Qubit dsDNA HS Assay Kit (Invitrogen, Waltham, MA, USA). DNA purity was verified (OD260/280 > 1.8 ...
-
bioRxiv - Physiology 2020Quote: ... total cholesterol E and non-esterified fatty acid (NEFA) were measured with commercially available kits following manufacturers’ instructions: Infinity TG kit (Thermo Fisher Scientific), total cholesterol E kit (Wako Diagnostics) ...
-
bioRxiv - Microbiology 2020Quote: ... The DNA concentrations were measured fluorometrically (Qubit® 3.0, kit Qubit® dsDNA Broad Range Assay Kit, Life Technologies, Carlsbad, CA, USA). Purified DNA from 35 isolates was prepared for sequencing using Nextera XT DNA Library Prep Kit (Illumina) ...
-
bioRxiv - Microbiology 2021Quote: ... RT-qPCR were performed with SuperScript III Platinum One-Step qRT-PCR kit (SuperScript™ III Platinum™ One-Step qRT-PCR kit, universal Invitrogen) using 5μL of nucleic acid extract and 20μL of RT-qPCR reagent mix using cycling parameters ...
-
bioRxiv - Microbiology 2021Quote: ... RT-qPCR were performed with SuperScript III Platinum One-Step qRT-PCR kit (SuperScript™ III Platinum™ One-Step qRT-PCR kit, universal Invitrogen) using 2.5μL of nucleic acid extract and 7.5μL of RT-qPCR reagent mix ...
-
bioRxiv - Bioengineering 2021Quote: ... DNA contents were measured using the Quant-it PicoGreen double-stranded DNA Kit (Quant-iT™ PicoGreen™ dsDNA assay kit, Cat. No. P7589, Invitrogen). Briefly ...
-
bioRxiv - Cancer Biology 2020Quote: Total RNA was isolated from snap-frozen (liquid nitrogen) tissues using a Qiagen RNeasy Plus Isolation Kit followed by cDNA synthesis (High-Capacity cDNA RT kit, Applied Biosystems, 4374966). RT-PCR was performed using TaqMan Universal Master Mix II (Fisher ...
-
bioRxiv - Cell Biology 2020Quote: ... CRISPR gRNAs were synthesized using a MEGAshortscript T7 Transcription Kit and purified using a mirVana miRNA Isolation Kit (Ambion, Austin, TX, USA). Zebrafish codon-optimized cas9 was synthesized using a mMESSAGE mMACHINE Kit (Ambion ...
-
bioRxiv - Neuroscience 2021Quote: ... rRNA were depleted from 1 μg of total RNA using the NEBNext rRNA depletion kit (Huma/Mouse) and ribo-depleted RNA was quantified by Quant-iT ™ RiboGreen ™ RNA Assay Kit (Invitrogen). Using a reverse transcriptase and random primers ...
-
bioRxiv - Microbiology 2019Quote: ... Ilumina libraries were prepared using the Kapa Hyper prep kit as described (41) and quantified using Qubit dsDNA BR Assay Kit (Thermo Fisher Sc). Fragment size was assessed on a Fragment Analyzer (Advanced Analytical Technologies) ...
-
bioRxiv - Microbiology 2020Quote: ... The amplified band was purified using a Qiagen gel purification kit and TA cloning was performed using a TOPO™ TA cloning™ kit (Invitrogen). 12 clones were randomly picked for Sanger sequence analysis by the Advanced Genomics Core at the University of Michigan.
-
bioRxiv - Immunology 2021Quote: ... CD4+ and CD8+ T cells were isolated by negative selection with the Dynabeads Untouched Human CD4 T Cells Kit and the Dynabeads Untouched Human CD8 T Cells Kit (Invitrogen, Thermo Fisher Scientific) respectively ...
-
bioRxiv - Immunology 2021Quote: ... total RNA was isolated using the Rneasy Mini Kit (cat# 74134) and cDNA was synthesized using High Capacity cDNA Reverse Transcription Kit (ThermoFisher, cat#4368814). Quantitative PCR was performed on synthesized cDNA samples using ThermoFisher TaqMan Master Mix (cat# 4444556 ...
-
bioRxiv - Neuroscience 2021Quote: ... Library quality was determined with the Agilent High Sensitivity DNA kit on an Agilent Bioanalyzer and concentrations measured with the Qubit™ dsDNA HS Assay Kit (Thermo Fisher) on a QubitTM (Thermo Fisher) ...
-
bioRxiv - Neuroscience 2022Quote: ... and control mice (Shank3b+/+ and Cntnap2+/+) with RNeasy Mini Kit (QUIAGEN) and retro-transcribed to cDNA with a SuperScript™ VILO™ cDNA Synthesis Kit (Invitrogen) according to the manufacturer’s protocol ...
-
bioRxiv - Developmental Biology 2022Quote: Bar-coded small RNA sequencing libraries were generated using the Ion Total RNA-Seq Kit v2 and the Ion Xpress RNA-Seq bar coding kit (Thermo Fisher Scientific). For the developmental course samples (Figure 1A) ...