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Citations for Thermo Fisher :
3251 - 3300 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 2 mL of B-27® Serum-Free Supplement (Gibco®, 17504), and 1 mL of GlutaMAX™.
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bioRxiv - Neuroscience 2022Quote: ... or cholera toxin subunit B (CTB; 2 mg/ml in PBS, Invitrogen). Once penetrating the surface ...
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bioRxiv - Neuroscience 2022Quote: ... we bilaterally injected cholera-toxin B (2%, #C34775, #C34778, ThermoFisher, Waltham, MA), a retrograde tracer ...
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bioRxiv - Bioengineering 2023Quote: ... supplemented with 2% (v/v) B-27™ supplement (Thermo Fisher Scientific), 1% (v/v ...
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bioRxiv - Neuroscience 2023Quote: ... 1X B-27 supplement and 2 mM L-glutamine (all from Gibco). Microglia were seeded on Poly-D-lysine(PDL)-coated coverslips (40.000-80.000 cells/cm2 ...
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bioRxiv - Genomics 2023Quote: ... and on day 2 with DE induction Media B (Gibco Cat# A3062601). On day 3 ...
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bioRxiv - Immunology 2023Quote: ... B cells were stained with 2 μg/ml Indo-I (Catalog:I1203, Invitrogen) in RMPI medium for 30 mins at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: ... DMEM was replaced with Neurobasal medium supplemented with 2% B-27 (Invitrogen), 1 mM Ala-Gn (Glutamax ...
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bioRxiv - Microbiology 2020Quote: ... 20 μl RNase A/T1 mix (2 μg μl−1 / 5 U μl−1, Thermo Scientific) was added to 100 μl lysate (OD260 ~ 150 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then washed three times and stained with 10 µM SYTO 9 (Invitrogen, USA) in PBS for 10 minutes ...
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bioRxiv - Genetics 2022Quote: ... 9 and 10 levels were analyzed using a TSQ Altis triple quadrupole mass spectrometer (ThermoFisher) coupled to a Vanquish LC system (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... were three-fold diluted (ranging from 1:9 to 1:243) in DMEM (Gibco) supplemented with 10% heat-inactivated FCS (Gibco) ...
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bioRxiv - Developmental Biology 2021Quote: ... with 50-100 nl of 1 mM 5-ethynyl-2’-deoxyuridine (EdU, Invitrogen) at stage 41 ...
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bioRxiv - Biophysics 2022Quote: ... NPE-caged ATP (adenosine 5’-triphosphate, P3- (1-(2-nitrophenyl ethyl) ester) (Invitrogen) dissolved in attachment buffer was photolyzed in the AFM chamber using an UV light source at 340 nm ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were pulsed with 1 mM 5-Ethynyl-2’-deoxyuridine (EdU, Invitrogen) for 3 hr ...
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bioRxiv - Cell Biology 2020Quote: ... cells previously seeded overnight were incubated for 4 h with 10 μM Alexa Fluor™ 594-dextran (Invitrogen, D22913) and analysed in live by laser confocal microscopy.
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bioRxiv - Genetics 2021Quote: ... 10 μg of protein were loaded onto a 15-well NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen), run at 100 V for 2.5 hours ...
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bioRxiv - Neuroscience 2021Quote: ... Cell transfections were carried out in 96-well plate with 1.0×10^4 target cells where each well received 100 ng DNA with a mix of Lipofectamine 3000 (Invitrogen) in Opti-MEM.
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Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... The tube was then centrifuged at 1600 rpm for 10 minutes at 4°C and the pellet resuspended in Hank’s Buffered Saline Solution (#14175095, Gibco, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2021Quote: ... for 60 minutes at 4 °C and the reaction was stopped with 10 μl SuperaseIn (Thermo Fisher Scientific AM2696). Ribosome-protected fragments were isolated using microspin S-400 (GE HealthCare ...
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bioRxiv - Bioengineering 2021Quote: ... Bacteria were washed and pelleted with PBS via centrifugation (13,180 × g at 4 °C for 10 min) with a superspeed centrifuge (ThermoFisher). Afterwards ...
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bioRxiv - Bioengineering 2021Quote: ... and was fixed in 10% neutral-buffered formalin (NBF) at 4 °C for 72 h and stored in PBS (pH 7.4; Gibco) at 4 °C for subsequent NMR spectroscopy and imaging experiments.
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bioRxiv - Microbiology 2020Quote: ... and equivalent amounts of protein were mixed with 4× LDS sample buffer and 10× reducing agent (Thermo-Fisher Scientific), followed by denaturation at 70 °C for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... Insoluble proteins were pelleted by centrifugation (15,000 x g for 10 min at 4°C) and supernatants were analysed using a UHPLC system (Vanquish, ThermoFisher) coupled online to a mass spectrometer (Q Exactive ...
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bioRxiv - Cell Biology 2021Quote: ... followed by electroporation using the Neon electroporation system with a 10-µl tip according to the manufacturer’s instruction (Pulse voltage: 1200 V, pulse width: 25, pulse number: 4, Invitrogen). The final concentrations of the annealed crRNA/tracrRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Chromatin was clarified by centrifugation at 21,000 g at 4°C for 10 min and precleared with 80 µl prewashed Dynabeads protein A (ThermoFisher) for 30 min at 4°C ...
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bioRxiv - Genetics 2021Quote: ... for 10 minutes at 70°C before loading into Bolt 4-12% Bis-Tris Plus gels (Thermo Fisher Scientific). Gels were run at 120V for 2-3 hours in Bolt MES SDS Running Buffer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... 10 μg of protein from extracts were separated in SDS–polyacrylamide gels 4–12% (NuPAGE Bis-Tris, Thermo Scientific) and transferred to nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... 10 μl of proteins obtained after TCA precipitation were separated by 4-12% polyacrylamide gradient gel electrophoresis (NuPAGE-Invitrogen) in 1X MES buffer (50 mM MES ...
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bioRxiv - Microbiology 2022Quote: ... at 90°C for 10 min before being run on a 4-12% acrylamide denaturing gel (ThermoFisher, #NP0323 NuPAGE). Proteins were then transferred onto a nitrocellulose membrane (ThermoFisher ...
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bioRxiv - Genetics 2022Quote: ... centrifuged for 5 minutes at 500xg at 4°C and resuspended in 70µl flow cytometry buffer (PBS, 10% ESC-grade FBS (Gibco), 0.5mM EDTA ...
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bioRxiv - Molecular Biology 2019Quote: ... HEK293T cells (4 × 106) in 100 mm dishes were transfected with 10 µg pDEST12.2-NSUN2-FLAG using Lipofectamine 2000 (Invitrogen), and cultured for 48–72 h at 37°C in 5% CO2 ...
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bioRxiv - Molecular Biology 2021Quote: ... For PhoX crosslinking we used a Fe3+-IMAC column (Propac IMAC-10 4 × 50 mm column, Thermo Fisher scientific) connected to an Agilent HPLC ...
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bioRxiv - Genomics 2020Quote: ... The chromatin was then cleared by centrifugation (12,000 g for 10 minutes at 4°C) and 80 μL of protein G DynaBeads (ThermoFisher, washed 3 times in cold ChIP buffer right before use ...
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bioRxiv - Synthetic Biology 2020Quote: ... 10-100 μg total protein was then separated with a 4-12% (v/v) gradient Bis-Tris gel (ThermoFisher) run in MES buffer system ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were cleared of insoluble material by centrifuging at 20,000 g for 10 min at 4°C and protein concentration was determined with the BCA Protein Assay (ThermoFisher). For immunoblotting 20-40 μg of protein in equal volumes were heated in LDS-sample buffer (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... 10 μg of protein was loaded into a 15-well NuPAGE 4-12 % Bis-Tris gel (Cat# NP0336BOX, ThermoFisher) and run in MES buffer (50 mM MES ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were heated to 95°C for 10 min and run on NuPAGE 4%-12% bis-tris gels (Invitrogen). Proteins were transferred to PVDF membranes (BioRad ...
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bioRxiv - Immunology 2022Quote: ... cells were centrifuged at 4°C at 200xg for 10 minutes and fixed with IC fixation buffer (Thermo Fisher) in 200 µL (100 µL IC Fixation buffer + 100 µL PBS ...
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bioRxiv - Cell Biology 2022Quote: ... was added to the samples and the samples transferred to a NuPAGE 4-12% Bis-Tris Gel 1.0 mm x 10 well (Invitrogen) for SDS PAGE gel electrophoresis ...
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bioRxiv - Biochemistry 2022Quote: ... Nuclei were lysed in 300 µL SDS buffer (50 mM HEPES pH 7.4, 10 mM EDTA pH 8.0, 4% UltraPure™ SDS Solution (24730020, Invitrogen), freshly added 1× cOmplete™ EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Developmental Biology 2022Quote: ... 10 µg protein of each sample was loaded into 4 to 12% Bis-Tris protein gel (Thermo Fisher Scientific) and sent for MS analysis at the University of Texas System Proteomics Network ...
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bioRxiv - Microbiology 2022Quote: Uninfected and MCMV-infected SVEC 4-10 were washed with 1X PBS and detached using TrypLE™ Express (Gibco) 18 hpi followed by blocking in 10% FCS-PBS (1X ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein concentrations of cell lysates were measured after centrifugation at 20,817g for 10 minutes at 4 °C using a Micro BCA Protein Assay Kit (Invitrogen). 50 μg of total proteins were analyzed by SDS-PAGE and Western blotting ...
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bioRxiv - Bioengineering 2022Quote: Equal concentrations of protein were loaded in all lanes of a 10% polyacrylamide gel with a 4% stacking gels were used and prepared per manufacturer’s instructions (Invitrogen). Pre-made ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were centrifuged at 1,000g at 4 ℃ for 10 mins and supernatants were then pre-cleared with 15 μl Dynabeads Protein G (Invitrogen) for 30 mins ...
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bioRxiv - Cell Biology 2022Quote: ... samples were separated on 10% or 4-20% gradient Novex™ WedgeWell™ Tris-Glycine gels (Thermo Fisher Scientific) for 40 minutes at 225V and transferred at 40V overnight at 4°C onto nitrocellulose membranes ...
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bioRxiv - Biochemistry 2024Quote: Reduced protein extracts were separated on 10% SDS–PAGE gels or 4–12% NuPAGE bis–tris precast gels (Invitrogen) by electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 µL of each sample was loaded into a well of NativePAGE 4-12% Bis-Tris Gels (Invitrogen NP0321BOX). The gels were run at 150 V for approximately two hours at 4 °C and transferred onto 0.2 µm PVDF membranes (Millipore IPVH00010 ...
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bioRxiv - Cell Biology 2024Quote: ... while 10 μg/lane of lysates were electrophoresed on Bolt 4–12% Bis-Tris Plus Gels (Thermo Fisher Scientific) for β-actin ...