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Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... then perfused the lungs with 3 mL of ice-cold DPBS containing Ca2+ and Mg2+ followed by 5 mL of 4% methanol-free formaldehyde (ThermoFisher) in DPBS containing Ca2+ and Mg2+ ...
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bioRxiv - Bioengineering 2019Quote: ... FDB fibers are stained at room temperature for 20 min prior to imaging with 5 µM of the calcium-sensitive dye mag-fluo-4 AM (KD ~22 µM, Invitrogen) dissolved in Tyrode’s buffer.
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bioRxiv - Plant Biology 2019Quote: ... and then rotated for another 5 min before loading the strip into the well of a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen).
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bioRxiv - Microbiology 2021Quote: ... boiled at 100°C for 5 min and then separated on NuPAGE Novex 4-12% Bis-Tris midi gels (Invitrogen) using NuPAGE MES SDS Running buffer (Invitrogen) ...
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bioRxiv - Developmental Biology 2019Quote: ... 50-100 μg of total protein extracts were solubilized in SDS-sample buffer by boiling at 95°C for 5 minutes and analysed by SDS polyacrylamide gel electrophoresis (4-12% NuPAGE gel; Invitrogen). Western blotting was performed using antibodies against Vasa (rat ...
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bioRxiv - Bioengineering 2019Quote: ... proteins were transferred onto a nitrocellulose membrane and then blocked overnight while shaken at 4°C in TBST-5% BSA – a TBS Tween-20 (TBST; ThermoFisher) solution containing 5% bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2021Quote: The input (total Nav1.5 expression) and biotinylated (cell surface-expressed Nav1.5) samples were separated on NuPAGE 4–12% Bis-Tris gels (Invitrogen, ThermoFisher) and transferred to nitrocellulose membrane (iBlot transfer system ...
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bioRxiv - Neuroscience 2021Quote: ... pH 6.8) for 5 min at 95° C and loaded to NuPAGE 4-12% Bis-Tris Protein polyacrylamide gels (Invitrogen, #NP0323BOX). After electrophoresis ...
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bioRxiv - Microbiology 2019Quote: ... The RNA was precipitated with ice-cold isopropanol and 3M sodium acetate (pH 5) at 4°C before RNA pellets were resuspended in nuclease-free H2O and treated with DNase I (Ambion) for 30 minutes at 37°C ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from groups of 5 HIOs per replicate with a total of 4 replicates per condition using the mirVana miRNA Isolation Kit (ThermoFisher). Cytosolic and mitochondrial ribosomal RNA was removed from samples using the Ribo-Zero Gold Kit according to manufacturer’s instructions (Illumina) ...
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bioRxiv - Immunology 2020Quote: ... Cells and virus were incubated for 4 hours at 37°C/5% CO2 and washed with PBS (Gibco, Waltham, MA) to remove excess virus ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were sonicated and aliquots containing 5 x 106 cells were separated on a NuPAGE bis-tris 4–12% gradient polyacrylamide gel (Invitrogen) under reducing conditions ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then separated by electrophoresis for a short amount of time (5 min) at 200 V on a NuPAGE 4-12% Bis-Tris gel in 1X MES/SDS (Invitrogen) running buffer ...
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bioRxiv - Immunology 2022Quote: ... 5-7 × 106 cells were resuspended in 3 mL of FACS buffer (4% FBS in phosphate buffered saline (PBS, Gibco)) and sorted at the University of Massachusetts Amherst Flow Cytometry Core Facility using a BD FACSAria Fusion (Becton Dickinson) ...
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bioRxiv - Microbiology 2022Quote: ... The RNA was precipitated with ice-cold isopropanol and 3 M sodium acetate (pH 5) at 4°C before RNA pellets were suspended in nuclease-free H2O and treated with DNase I (Ambion) for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... and washed again with PBS (4 x 5 min) before the slides were mounted using Prolong Gold Antifade (Thermo Fisher).
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bioRxiv - Physiology 2022Quote: HEK293-H cells were maintained in suspension at 37°C with 5% CO2 in CD293 medium supplemented with 4 mM GlutaMAX (Invitrogen). For imaging and electrophysiology ...
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bioRxiv - Bioengineering 2024Quote: ... MOLT-4 cells were maintained at 37°C with 5% CO2 in suspension culture in a complete growth medium (Gibco’s RPMI 1640 with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then incubated at 95°C during 5 minutes and separated by SDS-PAGE using 4-12% Bis-Tris gels (Invitrogen), stained for 1 hour using BioSafe-Coomassie stain (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... 4 μL of sample was applied and the grid was blotted for 5 seconds at 4 °C and 95% relative humidity then plunge-frozen with liquid ethane using a Vitrobot (ThermoFisher). Samples were clipped and screened by Glacios transmission electron microscope before 5,276 movies were collected on a Titan Krios transmission electron microscope at the Center for Electron Microscopy and Analysis (CEMAS ...
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bioRxiv - Molecular Biology 2024Quote: ... Villi were collected by centrifugation at 300 g for 5 min and single-cell suspension was generated by rotating in 20ml of 4% TrypLE solution (Invitrogen) in DMEM at 37°C for 30 min ...
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bioRxiv - Genetics 2024Quote: ... Samples were boiled at 95°C for 5 min before loading (60 µg) on 4-12% Bis-Tris gel (ThermoFisher) and ran with 1X MES buffer (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... filtered through a 5 µm strainer (04-004-2323, Sysmex) and washed with 4 ml of ice cold 0,01% BSA (AM2616, Thermo Fisher) in DPBS (59331C ...
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bioRxiv - Immunology 2023Quote: ... 10 μL of PageRuler Protein Ladder and 7 μg of each sample of proteins mixed with 5 μL of loading buffer were loaded and separated on a NuPAGE 4 to 12% Bis-Tris Gel (Invitrogen) using MOPS SDS Running Buffer 20X (Life technologies ...
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bioRxiv - Neuroscience 2022Quote: ... samples were mixed using either standard SDS Lämmli sample buffer and heated at 80°C for 5 min prior to loading onto gels (Novex Nupage 4-12% Bis- Tris gels, 1mm, 15 well, Invitrogen) and ran with Novex Nupage MES-SDS buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... while TSC were passaged every 4-5 days by washing with phosphate buffered saline (1xPBS) followed by brief incubation in 0.05% Trypsin-EDTA (Gibco, 25300054) at 37°C ∼2-3 mins) ...
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bioRxiv - Neuroscience 2023Quote: ... Then the samples were heated at 95°C for 5 min and loaded onto a pre-cast Polyacrylamide 4-12% gradient gel (Bolt, Invitrogen). The proteins were then transferred onto a nitrocellulose membrane and then blocked with 10% Blotto (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: Thermal shift assays were carried out using 4 μM PhPINK1 (residues 115–575) and 5× SYPRO Orange Protein Gel Stain (Invitrogen) in 25 mM Tris (pH 8.5) ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% CO2 in a humidified incubator and were passaged every 3-4 days using 0.05% Trypsin-EDTA (ThermoFisher Scientific, 25300). Stable U2OS-derived cell lines ...
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bioRxiv - Plant Biology 2023Quote: ... and then rotated for another 5 min before loading the strip into the well of a NuPAGE 4-12% Bis-Tris protein gel (Invitrogen).
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bioRxiv - Microbiology 2023Quote: ... 1ml of culture was pelleted and resuspended in 30μl 1X PBS buffer containing 5 µg/ml FM 4-64 (Invitrogen #T13320) and incubated for 2 min at room temperature in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 µg was loaded for each sample into a NuPAGETM 4-12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific, catalog #NP0336BOX) and run in NuPAGETM MES SDS running buffer (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were heated at 95°C for 5 minutes before loading on a 4-12% gradient Bis-Tris gel (Invitrogen). MOPS SDS running buffer (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were centrifuged at 500 g for 5 minutes at 4°C and red blood cells were hemolyzed using ACK buffer (Gibco). White blood cells were then incubated with 1:1000 Fc Block (AbLab ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... blocked in 5% donkey serum and incubated overnight at 4°C with a rabbit anti-Rbm20 antibody (PA5-53068, Invitrogen) at 0.5 μg/ml ...
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bioRxiv - Biophysics 2023Quote: ... denatured by heating for 5 min at 95°C and subjected to electrophoresis using precast NuPAGE Novex 4–12% Bis-Tris Proteins Gels (Invitrogen) in NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 100mM DTT and were heated at 95°C for 5 min prior to resolving (40% of the sample) on a 4-12% Bis-Tris NuPage NOVEX gradient gel (Invitrogen) in 1x Novex NuPAGE MES SDS Running Buffer (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... NIKWT and NIKKO BMDMs were treated with 20ng/mL IL-4 for 5 hours before cells were detached using PBS containing 1mM EDTA (Gibco, ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The first half was treated with 1x SDS loading dye for protein visualisation and incubated at 70°C for 5 minutes and loaded onto a WedgeWell Tris Glycine 4-12% gradient gel (ThermoFisher). Coomassie Brilliant Blue (CBB ...
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bioRxiv - Cell Biology 2024Quote: ... Cells received daily media changes and were passaged every 4-5 days as aggregates using 0.5 mM EDTA in DPBS (Cat # AM9260G, ThermoFisher Scientific). Cell aggregates were gently scraped off the bottom of the well and re-seeded onto a new Geltrex-coated dish.
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bioRxiv - Neuroscience 2023Quote: ... and cells were passaged every 4-5 days up to 70-85% confluency utilizing 0.5 mM EDTA (Thermo Fisher Scientific). On the first day post-subculturing ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were suspended in 710 µl of RIPA buffer with 7.5 µl PIC and incubated with gentle mixing overnight at 4 °C with 20 µl of DynabeadsTM Protein G (Invitrogen) coated with antibody against the analysed protein (listed in Supplementary Table S2) ...
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bioRxiv - Microbiology 2023Quote: ... Matched high and low molecular weight fractions adjusted to supernatant concentration equivalents in 0.9% saline were measured for fluorescence before and after addition (15 min incubation) of 5 μg/mL FM 4-64 (Invitrogen), and the background reading subtracted to obtain the final measurement.
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bioRxiv - Neuroscience 2023Quote: ... sliding microtome from lower lumbar segments (4 and 5) and processed free floating with Click-iT Alexa Fluor 488 (C10337, Invitrogen) or Click-iT Alexa Fluor 647 Imaging Kits (C10340 ...
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bioRxiv - Neuroscience 2023Quote: ... 5 ml of Fetal Bovine Serum (Fisher) and 125 μl of a 4 mg/ml human insulin stock solution (Gibco) were added ...
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bioRxiv - Biochemistry 2024Quote: HEK293 cells with Fzd1/2/4/5/7/8 knocked out were provided by Michael Boutros (Voloshanenko et al., 2017) and maintained in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gemini) ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspension was incubated with Fc block antibodies for 5 minutes at 4°C and then stained with Thy1.2-FITC antibody and IgG2a isotype antibody (Thermo fisher) as a negative control for 30 minutes at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were collected (5 minutes, 1,000 rpm, 4°C) and RNA was isolated using the PureLink™ RNA Mini Kit (Ambion). RNA concentration was measured using NanoDrop 8000 (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed in serum-free cRPMI medium and treated with 5 nM Fluo-4 calcium indicator dye (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... 4–5 × 104 cells were plated per well in 8-well Lab-Tek II Chamber Slides (Nunc, Rochester, NY, USA) one day before experiments ...