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Citations for Thermo Fisher :
3201 - 3250 of 10000+ citations for Heme oxygenase 1 HO 1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the spheroids were embedded to 20μl of matrigel and cultured in differentiation medium 1 (Diff1, 1:1 mixture of DMEM F12 and Neurobasal (Gibco #21103-049), 1x Glutamax ...
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bioRxiv - Neuroscience 2020Quote: ... or Alexa 555 (1:500) and donkey anti-rabbit IgG conjugated to Alexa 488 (1:500) or Alexa 555 (1:500) (Molecular Probes). The samples were mounted in Vectorshield (Vector Laboratory) ...
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bioRxiv - Cell Biology 2021Quote: ... followed by an anti-mouse secondary antibody (V5: 1 hr, RT, 1/500; Invitrogen, Cat #A21200; FLAG: 2 hr, RT, 1:200; Invitrogen, #A11004) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Orexin A/Hypocretin-1 (1:1000, R and D Systems, MAB763) Phospho-JUND (Ser255) (1:100, Thermo Fisher Scientific, PA5-104821), Phospho-nNOS (Ser1417 ...
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bioRxiv - Biochemistry 2022Quote: ... The reactions were stopped with addition of 1 μl 1% SDS and 1 μl 10 mg/ml Proteinase K (Invitrogen™), and incubating at 37°C for further 5 m ...
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bioRxiv - Cancer Biology 2020Quote: ... The UM92.1 cell line (a kind gift from Aart Jochemsen) were grown in a 1:1 mixture of RPMI 1640 (Gibco, Invitrogen) and DMEM/F12 (1:1 mixture ...
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bioRxiv - Neuroscience 2021Quote: ... and then the in secondary antibody solution (1% goat serum, 1% Triton X, and 1:500 goat anti-rabbit conjugated with Alexa Fluor 594, Thermo Fisher Scientific Cat# A-11037 ...
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bioRxiv - Physiology 2021Quote: ... followed by a 1 h RT incubation with PBS–1%BSA containing rabbit polyclonal RyR2 (PA5-36121, Thermo Fisher, 1:50) and/or rabbit polyclonal TOM20 antibody (Santa Cruz ...
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bioRxiv - Immunology 2020Quote: ... 100mM NaCl, 1 mM EDTA. 1% triton X-100 supplemented with Halt 1:100 protease Inhibitor cocktail Cat. No 78430, Thermo Scientific). After 30min on ice ...
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bioRxiv - Microbiology 2020Quote: ... The cell line was then adapted to suspension culture and maintained in Expi293™ expression medium supplemented with 1% (v/v) penicillin-streptomycin and 1 μg ml-1 puromycin (Gibco).
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bioRxiv - Microbiology 2020Quote: ... RNA samples (1 µg) were treated with DNase I (1 U/µl per 1 µg of RNA sample) (Thermo-Fisher Scientific), then reverse transcription carried out using the SuperScript™ IV first-strand synthesis system (Thermo-Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... 2.5 x105 CFSE-labeled CD8+ T cells were cultured with J774M cells at varying ratios (1:1 – 1:8 J774M:T cells) and anti-CD3/CD28 beads (ThermoFisher, cat. 11453D) at a bead-to-T cell ratio of 1:1 per the manufacturer’s instructions for T cell activation ...
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bioRxiv - Molecular Biology 2020Quote: RL-118 drug was diluted to a concentration of 20 μM (DMSO<1%) in tissue culture medium (DMEM with 10% FBS, 1% L-Glutamine, 1% penicillin-streptomycin, (Life Technologies)) ...
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bioRxiv - Neuroscience 2020Quote: ... a 1 µl Hamilton syringe (25s-gauge needle) filled with 150nL of 1% CT-B (1 mg/mL; Invitrogen, OR, USA) was used to pressure inject the retrograde tracer (150nL ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were subsequently washed with 0.05% PBST and stained with secondary antibody (Primary and Secondary antibody dilution provided in Table 1 Supplementary material) for 1 hr at RT and counterstained with DAPI (1:10000) (Life Technologies) for 1 minute and observed under fluorescent microscope Nikon Eclipse TE2000U.
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bioRxiv - Cell Biology 2022Quote: ... Retinas were then washed five times with 1:1 blocking buffer in PBS for 20 min and incubated with Alexa Fluor streptavidin-conjugated antibodies (1:100; Life Technologies) for 2 hours at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Phage DNA integrity was checked using agarose gel electrophoresis (1 % agarose gel in 1× TAE buffer; 70 V, 90 min) against a GeneRuler 1 kb ladder (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... generally according to the suppliers’ recommendations (1× EPAP buffer, 1 mM ATP, 1 mM DTT, and 0.8 U/μl RNaseOUT Recombinant Ribonuclease Inhibitor (Thermo Fisher Scientific), incubation at 37°C for 1 h) ...
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bioRxiv - Physiology 2022Quote: ... Mitochondria membrane polarization was measured by loading cells with 2 µM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, 15003) at 37°C for 15 min ...
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were washed 3 × 10 min with PBS and incubated in the primary antibody (Cx36 1:500, Chemicon MAB3045; p62 1:500, Abcam; Golgi97, 1:100, Thermo Fisher) diluted in 10% normal donkey serum in 0.5% Tx-100 (PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... at a density of 9×104 cells ml−1 in N2B27 differentiation medium containing Advanced DMEMF12 and Neurobasal media (1:1, Life Technologies), B27 w/o vitamin A (Life Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... Tissue sections were incubated with rat anti-mouse primary monoclonal mast cell protease-1 (MCPT-1) Ab (1:100, clone RF6.1; Thermo Fisher Scientific), followed by secondary staining with biotinylated rabbit anti-rat IgG (Abcam) ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Life Technologies a11006 ...
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bioRxiv - Immunology 2023Quote: ... the appropriate secondary antibodies were added diluted in 1% BSA in PBS for 1 hour at a 1:1000 dilution (Goat anti Rat 488 Thermo Fisher Scientific a11006 ...
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bioRxiv - Developmental Biology 2024Quote: mESCs were plated on gelatin-coated plates at a final density of 1 × 104 cells/cm2 in N2B27 medium (1:1 mix of DMEM/F12 (Gibco, 21331020) and Neurobasal medium (Gibco ...
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bioRxiv - Immunology 2023Quote: ... T cells were diluted to 1×106 cells/mL and activated with a 1:1 ratio of anti-CD3 and anti-CD28 Dynabeads (Gibco, 11141D). After 48h of incubation ...
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bioRxiv - Microbiology 2023Quote: ... Resuspended bacteria were then lysed (2 × 108 total CFU) by a 1:1 addition of 1% dodecyltrimethylammonium bromide (DTAB) (AC409310250; Fisher Scientific) for 5 min with agitation ...
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bioRxiv - Genomics 2023Quote: ... rabbit anti-H3K9me3 (1:200), rabbit anti-H3K27me3 (1:1000) (Frapporti et al., 2019) and mouse anti-FLAG (1:200) (Thermo Fisher Scientific Cat#MA1-91878 ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryo and skin sections were then incubated with the primary antibodies overnight at 4°C (LHX2, 1:1000, #3030529 Merck, Darmstadt, Germany; SOX9, 1:1000, #AB5535 EMD Millipore, Burlington, MA, USA; SOX2, 1:150, #14981182 Invitrogen), washed in washing buffer the next day ...
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bioRxiv - Microbiology 2023Quote: ... PA (1:250), PB2 (1:250), NS1 (1:250, PA5-32243 and NS2 (1:250, PA5-32234) were all purchased from Thermo Scientific, Rockford ...
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bioRxiv - Neuroscience 2023Quote: ... were exposed overnight at 4°C to free-floating sections at concentrations (1:500-1:1000) and incubated with corresponding fluorescence-conjugated secondary antibodies (1:1000; Invitrogen-ThermoFisherScientific). Sections were also treated with primary antibodies for Iba1 (to indicate microglial involvement ...
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bioRxiv - Neuroscience 2023Quote: ... 1:10, anti-mouse Minus-probe, 1:10, and anti-chicken Alexa Plus 647-secondary antibody, 1:1,000, Thermo Fisher Scientific) at 37°C for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: Frozen cell pellets from three independent rounds of differentiation were lysed in Tris buffered saline (TBS) containing 1% NP-40 and 1 mM freshly prepared MgCl2 and 1:100 Halt Protease inhibitor cocktail (ThermoFisher 78430). SDS was added to a final concentration of 1% and samples were boiled at 95°C for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Transfection was performed with 5 µg of pLKO.1 puro pri-mir-1-1 vector using Lipofectamine 3000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... in a 1:200 dilution in PBS with 0·1% (w/v) BSA and 0·1% (v/v) Triton-X100 (Thermo Fisher) at 4 ℃ overnight ...
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bioRxiv - Neuroscience 2024Quote: ... primary Ab Iba-1 (1:500, Wako Chemicals, 019-19741) and secondary Ab anti-rabbit Alexa Fluor 488 (1:500, Invitrogen, A11034); Sciatic nerves were imaged with the Leica DMI 4000B confocal microscope at ×20 magnification.
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bioRxiv - Immunology 2024Quote: ... Single cells were obtained by flushing the cell strainer with 6 mL complete medium (RPMI 1640 supplemented 10% FBS, 1% HEPES, 1% GlutaMAX, and 1% Penicillin–Streptomycin, all purchased from ThermoFisher Scientific).
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bioRxiv - Physiology 2024Quote: Protein was extracted from 20-25 mg of skeletal muscle tissue via manual homogenisation in 15 µL·mg-1 muscle 1× RIPA lysis buffer 1 (#J62524-AE, Thermo Fisher Scientific) containing 10 µL·mL-1 phosphatase inhibitor and 1 µL·mL-1 protease inhibitor cocktail (#78440 ...
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bioRxiv - Cell Biology 2021Quote: Primary human vein endothelial cells (HUVECs) were cultured in human endothelial SFM medium (Invitrogen) containing 20% foetal bovine serum ...
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bioRxiv - Cell Biology 2020Quote: ... Human CD4+ T cells were activated by Dynabeads Human T-Activator CD3/CD28 (ThermoFisher) and cultured in AIMV medium (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... KYAE1 cells were cultured in 1:1 RPMI 1640:F12 (Thermo Fisher, 11765054) supplemented with 10% foetal bovine serum and 1% penicillin/streptomycin ...
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bioRxiv - Genetics 2021Quote: ... COT-1 FISH was carried out using labeled mouse COT-1 DNA (Invitrogen). For quantification ...
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bioRxiv - Developmental Biology 2021Quote: ... and incubated with anti-ZO-1 (1:200, Thermo Fisher Scientific ref. 402200); acetylated tubulin (6-11B ...
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bioRxiv - Developmental Biology 2020Quote: ... N2B27 medium contained a 1:1 ratio of DMEM/F12:Neurobasal medium (GIBCO) supplemented with 1xN2 (GIBCO) ...
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bioRxiv - Neuroscience 2022Quote: ... 100 U ml-1 penicillin/streptomycin and 100 ug ml-1 G418 (Gibco), as previously described (12).
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bioRxiv - Molecular Biology 2021Quote: ... anti-Vsx2 (Chemicon, AB9016, 1:400) and anti-TUJ1 (Invitrogen, 480011, 1:250), and secondary antibodies included species-appropriate Alexa 488 (Invitrogen ...
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bioRxiv - Molecular Biology 2020Quote: ... for 1 hour at 90 V in 1 x NuPAGE transfer buffer (Invitrogen) containing 10% methanol ...
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bioRxiv - Neuroscience 2021Quote: ... and the secondary antibodies goat anti-rabbit 1:100 or 1:250 (Invitrogen). For all DNA stainings ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 mM of 4-(2-hydroxyethyl)-1-piperazine-1-ethanesulfonic acid (HEPES) (Gibco) and 1 ng/mL of human bFGF (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... with additional 1 mM CaCl2 and 1 mM MgSO4 (all Thermo Fisher Scientific) (see Watson et al. ...