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Citations for Thermo Fisher :
3451 - 3500 of 10000+ citations for Heme oxygenase 1 HO 1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... human Gas6 (Invitrogen, cat# BMS2291), mouse Axl (Abcam ...
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bioRxiv - Neuroscience 2022Quote: ... Human primary astrocytes (ThermoFisher #N7805200) were maintained as described in user manual ...
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bioRxiv - Molecular Biology 2024Quote: ... human COT1 DNA (#15279011, Invitrogen) and 3 volumes of ethanol (#10000652 ...
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bioRxiv - Immunology 2024Quote: ... or anti-human (Invitrogen #A11013) secondary antibodies at 20 μg/mL in PBS/2% BSA for 30 min ...
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bioRxiv - Systems Biology 2023Quote: ... and human IgE-PE (ThermoFisher). Antibody details are shown in Table S4 ...
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bioRxiv - Genomics 2023Quote: ... human p53 (Invitrogen, PA5-27822), MDM2 (Santa Cruz Biotechnology ...
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bioRxiv - Cancer Biology 2023Quote: ... human IFN-gamma ELISA (Invitrogen) and granzyme B ELISA (R&D Systems ...
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bioRxiv - Physiology 2023Quote: ... Human GR-specific siRNA (Invitrogen) was used at a concentration of 100 nM for 48 h to effectively knockdown GR ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-human CD235a (#17998742, Invitrogen), anti-human CD326 (EpCAM ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-human CD45 (#17945942, Invitrogen), anti-human CD235a (#17998742 ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant human EGF (Gibco #PHG0311) was added to the medium of NCI-H23 or H23 KO cells at 80% confluency to reach a final concentration of 100 ng/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... Human Pool Set v3.0 (Thermofisher) and TaqMan™ MicroRNA Reverse Transcription Kit (Thermofisher) ...
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bioRxiv - Genetics 2024Quote: ... human for rs2297550 from ThermoFisher.
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bioRxiv - Molecular Biology 2019Quote: ... the cells were washed three times in PBS and incubated in secondary antibody (2 mg/mL Alexa Fluor 488, Invitrogen, 1:1500, 2 mg/mL Alexa Fluor 594, Invitrogen, 1:1500; 2 mg/mL Alexa Fluor 568, Invitrogen, 1:1000) diluted in PBS for 1.5 h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... and hTERT RPE-1 (CRL-4000; ATCC) were cultured in Corning DMEM/Ham’s F-12 1:1 mixture (Fisher Scientific, Waltham, MA) with 10% FBS (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Dead cells were excluded by labeling using PI (1 mg/ml, 1:500) or near IR-fixable dye (1:1000, Invitrogen, cat. L34976). For the wash step ...
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bioRxiv - Biophysics 2022Quote: ... The cell culture medium (RPMI 1640) and complements (10% FBS, 1% Hepes 1M, 1% Glutamax, 1% Pen/Strep) were obtained from Gibco (Life technologies). Cells were monthly tested for the presence of mycoplasma.
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bioRxiv - Bioengineering 2022Quote: ... Prepared drug stocks were then diluted 1:100 in cell maintenance media (Gibco DMEM supplemented with 10% heat-inactivated FBS, 1% Gibco NEAA, 1% Gibco Pen-Strep) to achieve a 2x concentration ...
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bioRxiv - Developmental Biology 2020Quote: ... Pros (mouse 1:100, DSHB MR1A), Hairless (guinea pig 1:500, Annett and Dieter) and V5 (mouse 1:500, Invitrogen R960-25).
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bioRxiv - Biochemistry 2020Quote: ... wells were appropriately treated with anti-rabbit 594 antibody (1:1200, MilliporeSigma) for 1 hour at room temperature and then DAPI (1:1000, Thermo Fisher Scientific) for 15 minutes at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... and HA-mUNC79 plasmids or their various mutants (in a modified BacMam expression vector 27) at a ratio of 2:1:1:1 using Lipofectamine 3000 (Thermo Fisher Scientific) and incubated for 24 hours before recording ...
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bioRxiv - Cell Biology 2022Quote: ... Each cell lysate was mixed with Cellular ATP Kit HTS (155-050, BioThema) in the ratio of 1:1 and luminescence was analyzed using Microlite™ 1+ strips (Thermo Scientific) and Chameleon V (Hindex).
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bioRxiv - Molecular Biology 2022Quote: ... pLV-mCherry at ratio 1:1:1 (i.e. 5:5:5 μg) using Lipofectamin™ 3000 transfection reagent (Thermo-Fisher Scientific, #L3000015). Pseudoviruses harvested from the supernatant at 48 h 72h post-transfection were filtered (0.44 μm ...
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bioRxiv - Biophysics 2022Quote: ... and 1% penicillin/streptomycin (final concentration of 100 IU mL−1 penicillin and 100 μg mL−1 streptomycin, Gibco, Thermo Fisher, USA). Dry PIC polymers were sterilized by UV for 10 min and then dissolved in the medium for 24 h at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... and 1% penicillin/streptomycin (final concentration of 100 IU mL−1 penicillin and 100 μg mL−1 streptomycin, Gibco, Thermo Fisher, USA). Dry PIC polymers were sterilized by UV for 10 min and then dissolved in the medium for 24 h at 4 °C ...
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bioRxiv - Genetics 2021Quote: ... and stained with rabbit polyclonal antibodies against collagen XII (KR144;1:200), collagen VI (1:1000, gift from Raimund Wagener, Cologne) and Alexa 488 conjugated wheat germ agglutinin (WGA) (Invitrogen, 1:500), followed by corresponding secondary antibodies coupled to Cy3 (1:800 ...
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bioRxiv - Immunology 2020Quote: ... containing 1 µl 10x DNase I reaction buffer and 1 µl (1 U/µl) amplification grade DNase I (both from Invitrogen, Carlsbad, USA). The resulting mix was incubated for 15 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... fluence 0.6 μmol m−2 s−1) XccBphP wild-type samples at 1 mg mL−1 (14 μM) were incubated with bissulfosuccinimidyl suberate (BS3) (Thermo Fisher Scientific) in 250 ...
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bioRxiv - Cell Biology 2020Quote: ... cut into 1 × 1 cm pieces and incubated overnight in digestion medium containing 1 mg/ml collagenase type I (Gibco, Massachusetts, USA) and 100 μl/ml antibiotic/antimycotic solution in Modified Hank’s Balanced Salt Solution (HBSS ...
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bioRxiv - Immunology 2019Quote: ... in a 1:1 beat to T-cell ratio and cultured in 2 ml (24WP) or 1 ml (48WP) RPMI 1640 (Thermo Fisher, 21875) supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies Anti-FAS3 (Developmental Biology Hybridoma Bank (DSHB); 1:10) and anti-EcR (DSHB; 1:10) were used in conjunction with fluorescent Alexa-555- (ThermoFisher; 1:400) and Cy3- (Jackson Laboratories ...
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bioRxiv - Systems Biology 2020Quote: RNA-Seq libraries were prepared from 65 ng of total RNA and 1 μL of 1:5000 (v/v) dilution of ERCC ExFold RNA Spike-In Mix 1 (Thermo Fisher Scientific) with TruSeq® Stranded mRNA Library Prep for NeoPrep™ instrument as per the NeoPrep Library Prep System Guide on the instrument (Illumina).
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bioRxiv - Synthetic Biology 2020Quote: ... were grown on 0.1% gelatin-coated 35mm cell culture dish in EGM2 media (20% Fetal Bovine Serum, 1% penicillin-streptomycin, 1% Glutamax (Gibco, catalog #35050061), 1% ECGS (endothelial cell growth factors) ...
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bioRxiv - Cell Biology 2019Quote: ... For the negative control primary antibodies mouse anti-plasmepsin V (From D. Goldberg, 1:1) and rabbit anti-GFP A-6455 (Invitrogen, 1:200) were used.
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bioRxiv - Bioengineering 2021Quote: ... The primate ovaries were dissected into approximately 60 fragments measuring 1×1×1mm (1 mm3) and transferred into maintenance media (α- MEM, P/N 32561, Gibco, USA) prior to encapsulation (Figure 4) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for overnight with primary antibody at 4 degrees and further incubated with secondary antibodies (1:500) for 1 hour followed by 4′,6-diamidino-2-phenylindole (DAPI) or phalloidin 488 (1:400, Thermo Fisher, A12379) staining ...
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bioRxiv - Biochemistry 2021Quote: The three plasmids encoding the CST subunits were transfected into HEK293T cells at 1:1:1 molar ratio using lipofectamine 2000 (11668019, Thermo Fisher Scientific). The cells were further expanded (typically three-fold ...
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bioRxiv - Cell Biology 2020Quote: ... Droplets were solidified at 37°C and were grown four days without agitation in neurosphere medium containing a 1:1 mixture of DMEM/F12 and Neurobasal supplemented with N2 (1:200) (Thermo Fisher Scientific), B27 (1:100 ...
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bioRxiv - Immunology 2020Quote: ... T cell activation was performed using plate-bound α-CD3 (clone 145-2C11, 1 μg/ml) and α-CD28 (clone PV-1, 1 μg/ml) monoclonal antibodies in RPMI 1640 medium (Life Technologies) supplemented with 10% FCS ...
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bioRxiv - Molecular Biology 2020Quote: ... Rabbit antibody to pSMAD 1/5/8 (1:100; CST 9516) and mouse antibody to beta-amyloid (1:100; Invitrogen 13-200) were diluted in the same 3% blocking buffer and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... secondary antibodies (osteopontin: Alexa 546, 1:200, A21123, Invitrogen Molecular Probes; collagen type 1: Alexa 647, 1:200, A21246, Invitrogen Molecular Probes) were incubated with 0.1 μg/ml DAPI for 1 h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... secondary antibodies (osteopontin: Alexa 546, 1:200, A21123, Invitrogen Molecular Probes; collagen type 1: Alexa 647, 1:200, A21246, Invitrogen Molecular Probes) were incubated with 0.1 μg/ml DAPI for 1 h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... and subsequently incubated in primary antibody solution (1:200 CD31 Dako IS610 + 1:200 CD10 Invitrogen PA5-85875 or 1:200 anti-laminin Abcam ab11575 or 5 μg/mL ZO-1 Invitrogen #61-7300) overnight at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... incubated overnight with primary antibodies (1:5000 α-TNY1, 1:10,000 Roche Rat-anti-HA, high affinity 3F10, or 1:50,000 Invitrogen α-Histone H3) in 5% non-fat dry milk ...
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bioRxiv - Immunology 2022Quote: ... mBECs treated with IFNγ were fixed with 4% paraformaldehyde in PBS for 15 minutes and stained with antibodies against Zona occludens-1 (ZO-1, 1:250 Invitrogen 33-9100) and CXCL10 (1:500 Abcam ab9938) ...
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bioRxiv - Cancer Biology 2022Quote: ... complimentary to Ov-grn-1 exon 1 termed pCas-Ov-grn-1 was constructed using the GeneArt CRISPR Nuclease Vector kit (Thermo Fisher Scientific), as described ...
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bioRxiv - Cell Biology 2022Quote: HUVECs expressing PIK3CA p.H1047R or PIK3CA wt were transfected with a Silencer Select siRNA targeting to TGFA (ID: s14053) or negative control siRNAs (#1 and #2 mixed in ratio 1:1; all siRNA oligonucleotides from Thermo Fisher Scientific) as previously described (58) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated in rabbit polyclonal anti-Blbp (Suppl. Table 1) overnight followed by 1 hr in donkey anti-rabbit 555 (1:800; Life Technologies, #A31572) and coverslipped with Fluorogel ...
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bioRxiv - Neuroscience 2023Quote: Samples for lipidomics were resuspended in 25 µl chloroform/methanol (1:1) and 1 µl injected using a Vanquish Horizon UPLC (Thermo Fisher Scientific) equipped with a Waters ACQUITY Premier CSH (2.1 x 100mm ...
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bioRxiv - Cancer Biology 2024Quote: ... PC9 – CRUK0764 co-cultures were performed in a mixture of RPMI-1640 and DMEM (1:1) containing 1% fetal bovine serum (Gibco, #10270-106).