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Citations for Thermo Fisher :
3151 - 3200 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: The adeno-associated virus MyoAAV.2A was produced using the AAV-MAX Helper-Free AAV Production System Kit (Thermo Fisher Scientific). At 72 h post-transfection ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were infected (multiplicity of infection of 6 for each virus) with separate baculoviruses (prepared using the Bac-to-Bac Baculovirus Expression System, Invitrogen) containing the CXCL12LRHQ gene as well as the ACKR3 gene (residues 2-362 ...
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bioRxiv - Immunology 2023Quote: ... 2μg liver RNA per sample was used for qRT-PCR analysis for Plasmodium 18S rRNA using TaqMan Fast Virus 1-Step Master Mix (Applied Biosystems). Data were normalized for input to the GAPDH control for each sample and are presented as ratios of Plasmodium 18s rRNA to GAPDH RNA signals ...
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bioRxiv - Microbiology 2023Quote: We diluted freshly purified virus samples in DPBS and combined three volumes of the virus with one volume of NuPAGE LDS Sample Buffer (4×; Thermo Fisher) containing 100 mM dithiothreitol ...
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bioRxiv - Microbiology 2023Quote: ... For intravenous inoculation mice were restrained and injected via tail vein injection with 100 to 200 μl of virus or control inoculum using a 29G insulin syringe (Fisher Scientific). For natural transmission ...
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bioRxiv - Pathology 2023Quote: ... was reverse transcribed at 37°C for 50 min in a 20 µl reaction mix containing 200U Moloney murine leukemia virus (MMLV) reverse transcriptase (Life Technologies), 100 ng random primers ...
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bioRxiv - Genomics 2023Quote: ... the fibroblasts were thawed and plated at a density of 250K cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Biochemistry 2023Quote: ... Baculoviruses were amplified twice and freshly generated P3 virus was used to infect 1.8-2 L of High Five cells (Thermo Fisher Scientific) at a density of 3-3.5 x106 cells/mL by adding 6 mL virus per 600 mL of cells ...
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bioRxiv - Microbiology 2023Quote: ... Viral genome levels were quantified by qPCR using virus specific primer probe sets (described above) and normalised to GAPDH using an Applied Biosystems FAM/MGB probe mix (Thermofisher, 4333764F).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Patients consent was retrieved and the iPSCs were generated from peripheral blood mononuclear cells (PBMCs) using Sendai virus (CytoTuneTM-iPS 2.0 Sendai Reprogramming Kit, Thermo Fisher Scientific) as previously described ...
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bioRxiv - Neuroscience 2024Quote: ... The MS19-ES-H line was reprogrammed from PBMCs from a healthy female donor with a Cytotune Sendai Virus kit (Thermo Fisher).
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bioRxiv - Microbiology 2024Quote: ... isolated RNA was run using a TaqMan Fast Virus One-Step Master Mix (applied Biosystems) on a QuantStudio 3 Flex Real-Time PCR system (Applied Biosystems). RNA standards with known copy numbers were used to generate a standard curve and calculate sample copy numbers ...
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bioRxiv - Cancer Biology 2024Quote: ... Virus to construct stable HA-B56α overexpression cell lines were produced using HEK293T cells transfected using Lipofectamine 3000 (Fisher Scientific, L3000015) with the plasmid of interest (POI ...
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bioRxiv - Genetics 2021Quote: ... Cas9 protein (Invitrogen), a single-stranded 100-bp oligo donor nucleotide (ssODN ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein gel (Invitrogen). Antibodies were added according to the manufacturers recommended conditions ...
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bioRxiv - Biochemistry 2022Quote: ... The antibody-protein complexes were isolated by incubation with magnetic beads (Invitrogen Dynabeads protein A and protein G) for 6 hours at 4℃ ...
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bioRxiv - Physiology 2023Quote: ... supernatant protein concentration was determined by BCA protein assay (Pierce BCA Protein Assay Kit, Cat#23225, Thermo Scientific). Twenty μg/lane was run (200V ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant containing nuclear proteins was measured for protein concentration using Pierce BCA Protein Assay Kit (Thermo Scientific) and stored at -80°C until use ...
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bioRxiv - Bioengineering 2023Quote: ... Total protein concentration was determined using the Qubit™ Protein and Protein Broad Range (BR) Assay Kit (Invitrogen).
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bioRxiv - Bioengineering 2023Quote: Protein yield: MSC-EV preparation total protein concentration was determined using the Micro BCA Protein Assay Kit (ThermoFisher) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration of each sample was determined using the BCA protein assay (Pierce™ BCA Protein Assay, Thermofisher). 40 micrograms of protein per lane were prepared in SDS sample buffer (50 mM Tris pH 6.8 ...
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bioRxiv - Neuroscience 2024Quote: Protein concentration in the protein sample was determined by using a BCA protein assay kit (Pierce, Thermo Scientific). Samples in aliquots of 100 μL each were stored at -80°C.
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bioRxiv - Developmental Biology 2021Quote: ... was incubated for 1 h at RT and the signal was detected using SuperSignal West Pico PLUS chemiluminescent substrate (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... HPR-conjugated secondary antibodies were obtained from Cell Signalling and blots were developed with Super Signal West Dura (Thermo Fisher Scientific). Primary and secondary antibodies were removed by using Restore PLUS Western Blot Stripping Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2019Quote: ... The membrane was then incubated with a 1:1 mixture of SuperSignal West Pico Stable Peroxide solution and Luminol Enhancer solution (Thermo Scientific, Rockford ...
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bioRxiv - Immunology 2021Quote: ... Membranes were blocked with 5% BSA in TBST buffer and probed with anti-mouse Tim-3 goat polyclonal antibody (R&D) and detected with anti-goat-HRP antibody and SuperSignal West Pico Chemiluminescent reagent (Thermo Fisher).
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bioRxiv - Immunology 2021Quote: ... Bound antibodies were detected by reaction with a SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific, Grand Island, NY; Cat. No 34577).
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bioRxiv - Developmental Biology 2020Quote: ... The nitrocellulose membrane was washed three times in TBST before enhanced chemiluminescent (ECL) detection by SuperSignal West Dura Western Blot Substrate (Fisher Scientific) and exposure to autoradiography film.
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were incubated with horseradish Peroxidase-conjugated AffiniPure Goat Anti-Rabbit IgG and Goat Anti-Mouse IgG (ZSGB-BIO, Beijing, China) for 2 h and signals were observed using Super Signal West Pico (Thermo Scientific). Western blotting band intensity was quantified by densitometry analysis using ImageJ version 2x (NIH Image software ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... SuperSignal West Pico PLUS Chemiluminescent Substrate (Pierce, Rockford, IL) (or, for CD47, SuperSignal West Femto Maximum Sensitivity) was used for detection on an iBright FL1000 (Thermo Fisher) in chemiluminescence mode.
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bioRxiv - Neuroscience 2022Quote: ... The membranes were then exposed to peroxidase-conjugated anti-rabbit secondary antibody and SuperSignal West Femto Maximum Sensitivity Substrate (ThermoFisher Scientific). The resulting chemiluminescent signal was acquired with a Chemidoc Touch Imaging System (Bio-Rad) ...
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bioRxiv - Immunology 2022Quote: ... After washing 3x with PBS-T and 2x with PBS, SuperSignal West Pico PLUS Chemiluminescent Substrate (cat# 34580, Pierce) was used for detection with an iBright FL1000 (Thermo Fisher) imager in chemiluminescence mode.
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bioRxiv - Neuroscience 2021Quote: ... Blots were washed 3 times with PBST and then ATXN1 blots were washed with Super Signal West Dura (Thermo Fisher Scientific) while α-Tubulin blots were washed with Super Signal West Pico (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... the membranes were incubated with HRP-conjugated secondary antibody and exposed using SuperSignal West Pico or Femto PLUS Chemiluminescent Substrate (ThermoFisher Scientific) by film in a dark room.
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bioRxiv - Immunology 2020Quote: ... The signals were detected with enhanced chemiluminescence substrate (ECL West Pico Femto, Fischer Scientific) on a Fusion FX6 instrument (Fisher Scientific).
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bioRxiv - Biochemistry 2020Quote: ... or a Fusion FX imager (Vilber) using ECL Western Blotting Substrate (Pierce) or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific). Western blot data were quantitated using the Gel Analysis tool in ImageJ (NIH) ...
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bioRxiv - Genomics 2020Quote: ... Blotting with primary antibodies was carried out at 4°C overnight followed by incubation with the appropriate secondary antibodies and developed using chemiluminescent West Pico detection kit (Thermo Scientific). Western blot quantification was carried out by subtracting the background signal from the individual bands ...
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bioRxiv - Developmental Biology 2019Quote: ... Membranes were washed again in PTW and antibody detection was performed using the Supersignal West Pico or Femto chemiluminescent substrates (Thermo Scientific) and visualized using a ChemiDoc XRS system (Bio-Rad) ...
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bioRxiv - Genetics 2019Quote: ... Appropriate HRP-conjugated secondary antibodies were used for band detection with SuperSignal™ West Dura Extended Duration Substrate (Thermo Fisher Scientific) using an Alliance chemiluminescence imaging system (UVITEC Cambridge) ...
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bioRxiv - Microbiology 2019Quote: ... The blot was again washed five times for 5 minutes in 1X PBS and immediately developed using SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Appropriate HRP-conjugated secondary antibodies were used for band detection with SuperSignal™ West Dura Extended Duration Substrate (Thermo Fisher Scientific) using an Alliance chemiluminescence imaging system (UVITEC Cambridge) ...
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bioRxiv - Genomics 2020Quote: ... the membranes were washed three times in 13 ml PBS and the bound antibodies were detected using the SuperSignal West Femto maximum sensitivity substrate (Thermo Scientific) according to manufacturer’s protocol and the LI-COR Odyssey system (LI-COR Biosciences).
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bioRxiv - Bioengineering 2021Quote: ... the membrane was washed with TBS-T and subsequently developed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific) and imaged using a Bio-Rad ChemiDoc XRS+ System.
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bioRxiv - Biochemistry 2021Quote: ... Blots were then washed for 5 minutes in TBS and treated with either standard ECL substrate or SuperSignal West Femto high sensitivity substrate (ThermoFisher Scientific). Blots were imaged using a Bio-Rad ChemiDoc Imaging System (Bio-Rad Laboratories) ...
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bioRxiv - Biochemistry 2020Quote: ... One gel was stained with Coomassie Blue and other was transferred onto a nitrocellulose membrane and probed for peroxidase activity using SuperSignal West Femto Maximum Sensitivity Substrate reagent (Thermo Fisher). Heme transfer from Sfh5 donor to apo-myoglobin acceptor was monitored by peroxidase activity in the myoglobin bands (Feissner et ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed with PBS/ 0.1% Tween 20 twice for 30 min and developed using ECL SuperSignal West (Pico or Dura, Thermo Fisher Scientific) and visualized by using X-ray films (Kodak developer) ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were then washed three times with TBST and Immunocomplexes were detected with SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific). Images were taken using a Bio-Rad Gel Doc Imager system ...
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bioRxiv - Plant Biology 2021Quote: ... The membrane was rewashed with TTBS for 4 x 5 min and was developed for chemiluminescence (with agent SuperSignal West Pico, Thermo Scientific) using a LAS 4000 Image Analyzer (ImageQuant).
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bioRxiv - Plant Biology 2020Quote: ... Western blot detection was done using either PierceTM ECL western blotting substrate or SuperSignal™ West Femto Maximum Sensitivity Substrate (ThermoFisher). All the immunoblots were probed using appropriate antibodies as indicated in the figures ...
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bioRxiv - Synthetic Biology 2021Quote: ... Membranes were again washed three times with TBST followed by signal detection with either SuperSignal West Pico PLUS (Thermo Fisher; 34579) or SuperSignal West Femto (Thermo Fisher ...