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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for West Nile Virus Envelope Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: LC-MS/MS analyses were conducted using either a QExactive Plus Orbitrap (QE, RNase-digested polysomes) or a Velos Pro Elite Orbitrap (Elite, virus polysomes and HEK293 aggregates) mass spectrometer (Thermo Fisher) coupled online to a nanoAcquity UPLC system (Waters Corporation ...
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bioRxiv - Cell Biology 2022Quote: ... the unbound virus was removed by washing with infection media followed by agarose overlay with 1X MEM Temin’s modification (Thermo Fisher, 11935046) +0.3% agarose ...
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bioRxiv - Microbiology 2022Quote: Viral RNA in apical secretions was quantified using the TaqMan™ Fast-Virus-1 Step master mix (Thermo Fisher, Basel, Switzerland) with these cycling parameters ...
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bioRxiv - Microbiology 2022Quote: ... HuNoV RNA genome equivalents (GEs) were determined by reverse transcription-quantitative PCR (RT-qPCR) analysis using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and GII specific primer/probe sets (Kageyama et al. ...
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bioRxiv - Bioengineering 2022Quote: ... infected cells were counted and determined by evaluating GFP expression after 24 hours exposure to virus-antibody mixture using Attune NxT Acoustic Focusing Cytometer (Thermo Fisher). Half-maximal inhibitory concentration (IC50 ...
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bioRxiv - Plant Biology 2021Quote: ... and then incorporated into the destination vector pMV3 (driven by the cauliflower mosaic virus (CaMV) 35S promoter) using the LR recombination reaction (Invitrogen, USA). All the recombinant constructs were transformed into the Agrobacterium tumefaciens strain C58 by electroporation and subsequently transformed into the tomato genome via explants of cotyledon ...
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bioRxiv - Developmental Biology 2021Quote: ... The virus was spun at 500 g for 5 min and the supernatant filtered through a 45 μm polyethersulfone filter (Fisher Scientific) and subsequently concentrated ...
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bioRxiv - Microbiology 2021Quote: ... viral stocks were prepared after two serial passages of isolated virus in Vero E6 cells in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) supplemented with 5% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Sf9 transfection with bacmid DNA and virus amplification were performed essentially as described for the Bac-to-Bac Baculovirus Expression System (ThermoFisher Scientific). Sf9 cells were maintained in Hyclone CCM3 (CCM3 liquid medium with L-glutamine ...
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bioRxiv - Immunology 2019Quote: ... and cDNA was reverse transcribed using the Moloney murine leukemia virus reverse transcriptase and random hexamer oligonucleotides for priming (Life Technologies). The expression of CPT1 was determined using PCR SYBR Green sequence detection system (Eurogentec ...
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bioRxiv - Systems Biology 2022Quote: All induced pluripotent stem cell (iPSC) lines were reprogrammed by the Sendai virus expressing 4 Yamanaka factors (CytoTune®-iPS Sendai Reprogramming Kit, Invitrogen) as previously described36 ...
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bioRxiv - Cancer Biology 2022Quote: ... except that some preparations were made using donor T cells that were activated for 48 hours before virus infection using LentiBoost (5 μl each of solutions A and B per ml of cells transfected; Fisher Scientific). 100 units/ml IL-2 was added at the start of the activation procedure and with every media change during the expansion phase.
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bioRxiv - Neuroscience 2019Quote: cDNA sequences from the leader through the first half of the rabies virus P gene were amplified using Platinum SuperFi Green Master Mix (Invitrogen (Thermo Fisher), USA ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and PCR amplification with virus candidate specific primers using the Platinum™ SuperFi™ DNA polymerase (Invitrogen™, Catalog number: 12359010). Amplified products were Sanger sequenced using intermediary primers enabling a full-length coverage (all primers are listed in Table S5).
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bioRxiv - Cell Biology 2020Quote: ... centrifuged at approximately 250 × g for 5 min at 4 °C and resuspended in thawed Geltrex™ LDEV ([lactose dehydrogenase elevating virus]-Free Reduced Growth Factor Basement Membrane Matrix; Gibco). Two 15-μl drops of the matrix-cell suspension were pipetted into wells of a Nunc™ 24-well-Nuclon Delta-treated plate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Cells were washed three times with PBS to remove residual input virus and overlaid with DMEM high glucose media (Life Technologies) with 0.7% low IgG BSA (Sigma) ...
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bioRxiv - Bioengineering 2020Quote: ... vRNA levels were determined by quantitative real-time PCR (qRT-PCR) using Applied BiosystemsTM TaqManTM Fast Virus 1-Step Master Mix (Thermofisher #4444432) and a primer-probe combination recognizing a conserved region of gag (GAG5f ...
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bioRxiv - Plant Biology 2021Quote: ... The identified gene fragments were then PCR-amplified from whitefly cDNA and cloned into a binary tobacco rattle virus VIGS vector pTRV2 (Liu et al., 2002) using the Invitrogen Gateway recombination cloning technology (Invitrogen, USA). As a positive control ...
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bioRxiv - Microbiology 2020Quote: ... apical poles were gently washed with warm PBS and then infected with a 150-μL dilution of HRSV-A (Long) virus in OptiMEM medium (Gibco, ThermoFisher Scientific) at a MOI of 1 ...
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bioRxiv - Microbiology 2020Quote: Zika virus RNA yields were assessed using real-time PCR on a 7500 Fast Real-Time PCR instrument (Thermo Scientific, Poland). ZIKV cDNA was amplified in a reaction mixture containing 1× TaqMan Universal PCR Master Mix (RT-PCR mix ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: RNA extraction and RT-PCR were performed as previously described.45 Total RNA was extracted from HCoV-NL63 virus infected Caco-2 cells at a MOI of 0.05 at 48 hours post infection using TRIzol reagents (Thermo Fisher Scientific). 2.0□μg of total RNA was used to synthesize the first strand cDNA of viral RNA and host RNA using SuperScript III reverse transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2019Quote: Peripheral blood CD34+CD38- cells mobilized from healthy donors were reprogrammed into iPSCs (MCND-TENS2 line) using the integration-free CytoTune 2.0 Sendai virus reprogramming kit (A16517, Thermo Fisher Scientific) following the method previously reported30,31 ...
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bioRxiv - Neuroscience 2020Quote: Total RNA (~40 μg per reaction) was reverse-transcribed and PCR amplified using Taqman Fast Virus 1-Step Master Mix (Applied Biosystems) and primetime assays for specific genes (IDT) ...
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bioRxiv - Microbiology 2021Quote: ... was derived from Vero cells infected with the virus and cDNA was synthesized using the SuperScript III Reverse Transcriptase (Life Technologies) and oligo dT and six hexamer random primers ...
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bioRxiv - Microbiology 2020Quote: ... viral stocks were prepared after two serial passages of isolated virus in Vero E6 cells in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) supplemented with 5% fetal bovine serum (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: SADS-CoV spike glycoprotein (virus strain GDS04; GenBank No.: ASK51717.1) gene was synthesized with codons optimized and inserted into pFastBac vector (Life Technologies Inc.). The ectodomain of SADS-CoV spike protein without the transmembrane anchor and intracellular tail (residues 18-1068 ...
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bioRxiv - Immunology 2020Quote: ... Ebola virus Makona Glycoprotein (EBOV-GP) (KM233102.1) was synthesized and cloned into pcDNA3.1 by GeneArt AG technology (Life Technologies, Regensburg, Germany) or VSV-G following the manufacturer’s instructions of Lipofectamine 3000 (Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The eluted RNA was combined with Applied Biosystems™ TaqMan™ Fast Virus 1-step master mix (ThermoFisher, Waltham, MA, USA). The target regions for the CSG ...
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bioRxiv - Microbiology 2021Quote: ... 5 μl of the RNA extraction final elution was reverse-transcribed to cDNA and amplified according to the manufacturer’s protocol using TaqMan™ Fast Virus 1-Step Master Mix (ThermoFisher Scientific). The primer pair was as follows ...
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bioRxiv - Immunology 2020Quote: ... Virus containing supernatants were harvested 2 days later and the produced infectious virus yield was measured using TZM-bl reporter cell line and Gal-Screen kit from Applied Biosystems as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... Induced pluripotent stem cells were derived from human fibroblasts using Sendai virus-based reprogramming kit (CytoTune-iPS Sendai Reprogramming Kit; cat #. A13780-01; Thermo Fisher), according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and number of virus-infected cells (based on dsRNA and nucleocapsid staining) was performed using the CellInsight CX7 HCS platform (Thermo Fisher), as previously described 55,56 ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatant containing the virus was then used fresh or stored by snap-freezing aliquots supplemented with 8 μg/ml polybrene (Fisher Scientific) in ethanol/dry ice and stored at -80° C ...
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bioRxiv - Cell Biology 2022Quote: ... Transduction efficiency was assessed by GFP expressing virus and subsequent quantification of GFP-positive cells by Countess II FL Cell Counter (Thermo Fisher).
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bioRxiv - Microbiology 2022Quote: ... RNA was extracted from 50 μl of virus inoculum suspension using the PureLink Viral RNA/DNA Mini kit (Themo Fisher Scientific), as per manufacturer’s directions.
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bioRxiv - Microbiology 2022Quote: ... and resuspended in thawed Geltrex lactose dehydrogenase elevating virus free (LDEV-free) reduced growth factor basement membrane matrix (Thermo Fisher Scientific). Three 10-μl drops of the matrix-cell suspension were pipetted into wells of a Nunclon Delta 24-well plate (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... 7 µl total RNA was amplified in a mix containing 5 µl of TaqMan Fast Virus 1-step master mix (Life Technologies), 0.4 µl primer-probe mix for IAV 40 and 9.6 µl distilled water ...
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bioRxiv - Microbiology 2022Quote: Copies of the SARS-CoV-2 N gene (genomic) were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Applied Biosystems). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: Copies of SARS-CoV-2 E gene (subgenomic) were measured by qRT-PCR with TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes sgLeadSARSCoV2-F 5’-CGATCTCTTGTAGATCTGTTCTC-3’ ...
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bioRxiv - Microbiology 2022Quote: ... The virus-containing samples (50 μl) were serially diluted in 450 μl aliquots of serum-free Opti-Pro medium (Gibco, UK), after which 200 μl from each dilution were added in duplicates to the cells ...
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bioRxiv - Microbiology 2022Quote: ... 5 µl total RNA was amplified in a mix containing 5 µl of TaqMan Fast Virus 1-step master mix (Life Technologies), 1 µl primer-probe mix for β-actin ...
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bioRxiv - Neuroscience 2022Quote: ... HT-1080 cells (ATCC CCL-121; RRID:CVCL_0317, male) used for virus titration were grown and maintained in MEM (Gibco Cat#11095-080) supplemented with 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... The samples were used for quantitating HuNoV RNA GEs by means of reverse transcription-quantitative PCR (RT-qPCR) analysis using a TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher) and GII specific primer/probe sets ...
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bioRxiv - Neuroscience 2024Quote: ... HEK cells below passage 10 are plated into a 15 cm dish/virus (Fisher 08-772-24) in DMEM-H (DMEM-h (invitrogen 10569010) + NEAA (Invitrogen 11140050) + 10% FBS (invitrogen 16140071 ...
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bioRxiv - Microbiology 2024Quote: ... Virus stocks were grown in M2-10B4 cells (ATCC # CRL-1972) with RPMI 1640 Medium special formulation (Thermo Fisher cat # A1049101) supplemented with 10% fetal bovine serum (Thermo Fisher cat # 12483020 ...
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bioRxiv - Cancer Biology 2024Quote: ... Subsequent qPCR was performed using the TaqMan Fast Virus 1-Step Master Mix and StepOnePlus Real-Time PCR System (Thermo Fisher). The primers purchased from TaqMan were ...
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bioRxiv - Genetics 2024Quote: The derivation of iPSCs was carried out by the NHLBI iPSCs core facility using the Cytotune 2.0 Sendai virus kit (ThermoFisher Scientific, A16517) with a few modifications for high throughput reprogramming essentially as described earlier (19) ...
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bioRxiv - Microbiology 2023Quote: ... Anesthetized mice were inoculated intranasally with 30 μL of virus diluted in chilled 1X PBS (MHV) or administered undiluted in 2% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Both assays were performed using the Taqman Fast Virus 1-Step Master Mix RT-qPCR kit (Applied Biosystems Inc., Carlsbad, CA) on an LC96 instrument (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... the elutes of GlnR147 (his-TEV GlnR C60SL147C) from Ni-NTA column were cleaved with recombinant tobacco-etch virus protease (Life Technologies) overnight at 4 °C ...