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Citations for Thermo Fisher :
3151 - 3200 of 9391 citations for Mumps Virus Nucleoprotein Strain L Zagreb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The protein concentration of the purified virus preparations was determined using a BCA protein assay kit (Thermo Fisher Scientific) prior to the addition of lysis buffer ...
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bioRxiv - Microbiology 2022Quote: ... and then quantified by qRT-PCR using the TaqMan™ Fast Virus 1-Step Master Mix (Thermo Fisher Scientific) with the TaqMan™ 2019nCoV Assay Kit v1 (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... The first strand cDNA was obtained using oligo(dT) primers and Moloney murine leukaemia virus-reverse transcriptase (Life Technologies), using 100 ng of purified RNA ...
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bioRxiv - Cell Biology 2024Quote: ... Virus was removed 16-20 hours following transduction with fresh neural media [Neurobasal medium (NBM; Life Technologies, #21103-049) supplemented with 1x N-2 supplement 1x B-27 supplement ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was removed after 24 h and cells were cultured in fibroblast medium with 10% FBS (Gibco, 16000-044) in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was diluted in sterile 0.9% w/v sodium chloride in low protein binding collection tubes (Life Technologies Ltd) and stored at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was discarded and virus pellet was resuspended in the same volume of RIPA buffer (Cat #89901, ThermoFisher, 20 mM Tris-HCl ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Batches of ten 8-10 weeks old female BALB/c mice were inoculated intraperitoneally (i.p) with 105 PFU of Addavax-adjuvant recombinant virus in 200 μL of phosphate-buffered saline (PBS; Gibco). At twenty-one days post immunization ...
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bioRxiv - Microbiology 2024Quote: ... A virus growth medium consisting of infection media supplemented with an additional 1 µg/mL trypsin-TPCK (ThermoFisher, USA) was added to the cells ...
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bioRxiv - Biochemistry 2024Quote: ... were infected with the virus at a cell density of 4.0 × 106 cells per milliliter in Sf900 II medium (Gibco), and grown for 48 h at 27 °C ...
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bioRxiv - Bioengineering 2024Quote: The virus riboswitch (vRibo) RNAs were synthesized using a TranscriptAid T7 High Yield Transcription Kit (Thermo Scientific Cat# K0441) from the DNA templates generated by PCR ...
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bioRxiv - Microbiology 2023Quote: The RNA of H1N1pdm09 was extracted from virus samples using a PureLink™ viral RNA/DNA mini kit (Invitrogen). For qRT-PCR ...
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bioRxiv - Cell Biology 2023Quote: ... Moloney murine leukemia virus (M-MLV) reverse transcriptase and RNaseOUT Recombinant Ribonuclease Inhibitor were purchased from Invitrogen (Carlsbad, CA). The Phospho-Akt (Ser473 ...
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bioRxiv - Microbiology 2023Quote: ... Trypsin used for virus propagation was standard cell culture grade 0.25% porcine trypsin with EDTA and phenol red (ThermoFisher). Huh-7 cells were seeded on a 24-well plate and incubated at 37 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... Viral plaques were passaged at an MOI of ∼0.001 in MDCK-II cells in virus growth medium comprised of Opti-MEM (Gibco), 2.5 mg/ml bovine serum albumin (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2023Quote: ... Virus stocks were prepared by three passages in Vero (ATCC CCL-81) in Dulbecco’s modified Eagle Medium (DMEM) (Gibco) with 1% Penicillin-Streptomycin (Gibco) ...
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bioRxiv - Biophysics 2023Quote: ... Negatively stained virus particles were observed on a 30 kV scanning electron microscope (Thermo Fisher Scientific, Quanta FEG 650) in scanning transmission electron microscopy (STEM ...
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bioRxiv - Cell Biology 2023Quote: ... HT914 and HT915 iPSC lines) using the integration-free CytoTune 2.0 Sendai virus reprogramming kit (A16517, Thermo Fisher Scientific) as previously reported [43–45] ...
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bioRxiv - Microbiology 2023Quote: ... The RT-PCR was performed using the Taqman Fast Virus 1-step master mix (Applied Biosystems, Foster City, CA) on a QuantStudio3 (ThermoFisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells infected with pLENTI-Luciferase-expressing virus (generous gift of Charlotte Kuperwasser, Tufts University) were selected with neomycin (G418, 500μg/mL, Gibco) for 2-3 weeks ...
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bioRxiv - Molecular Biology 2023Quote: ... MED1-IDR containing plasmid was obtained from Addgene #145276 and inserted into pcDNATM6.2-C-EmGFP-DEST supplemented with the Simian Virus 40 NLS sequence by Gateway strategy (Invitrogen) using primers described in Suppl ...
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bioRxiv - Microbiology 2023Quote: ... were infected with HSV-1 at MOI 0.01 by replacing cell media with virus diluted in minimal media Opti-MEM (ThermoFisher). The virus was allowed to adhere to cells at 37 °C in 5% CO2 for 1 hour with minimal agitation every 15 minutes ...
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bioRxiv - Microbiology 2023Quote: ... MRC-University of Glasgow Centre for Virus Research) cells were grown in Dulbecco’s Modified Eagle Medium (DMEM; ThermoFisher, 10566016) supplemented with 10 % fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA was reverse-transcribed using Moloney Murine Leukemia Virus (M-MLV) Reverse Transcriptase (RT) according to the manufacturer’s instructions (Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we extracted virus from the pooled samples from each assay grid using a sterile pellet pestle (Fisher Scientific, U.S.A.) to manually burst larvae and release viral occlusion bodies ...
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bioRxiv - Cell Biology 2024Quote: ... the virus titer was quantified using quantitative polymerase chain reaction (qPCR) with SYBR Green Master Mix (Thermo Scientific, 4309155) and primers targeting ITRs (Supplementary Table 1) ...
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bioRxiv - Microbiology 2024Quote: RNA was extracted from the supernatant of MDCK cells infected with the PB2 TRAP V5 virus using Trizol (Invitrogen). RNA was reverse-transcribed with Superscript III (Invitrogen ...
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bioRxiv - Biophysics 2024Quote: ... at a 6:1:2 ratio for each virus using the Bac-to-Bac baculovirus expression system (Thermo Fisher). Cell cultures were grown in ESF 921 medium (Expression Systems ...
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bioRxiv - Immunology 2024Quote: ... RNA was then reverse transcribed from 200 ng total RNA with Moloney murine leukemia virus reverse transcriptase (Invitrogen #28025013) using oligo (dT)18 (Thermo Scientific #SO131 ...
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bioRxiv - Physiology 2024Quote: ... and infected with sendai virus (SeV) carried polycistronic Klf4-Oct3/4-Sox2 and Klf4 vectors (ThermoFisher, Waltham, MA, #A16517), here referred as OSK-SeV ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... For metabolic labelling experiments with m+4Met under all conditions mPSC basal medium was generated with 0.2 mM L-Methionine- and L-Cysteine-free DMEM (Sigma or Life Technologies) supplemented with m+4Met ([methyl-13C,d3] ...
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bioRxiv - Molecular Biology 2021Quote: ... the cell pellets were resuspended with 50 μL of SC−Ura medium without carbon source (7.4 g/L Yeast nitrogen base without amino acids, #291940, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Single colonies were picked and streaked on YPAD agar plate and SCDex−Ura agar plate (20 g/L glucose, #16806-25, Nacalai tesque; 6.7 g/L Yeast nitrogen base without amino acids, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... and spread on a SCGal−Ura agar plate (20 g/L galactose, #075-00035, FUJIFILM Wako Chemicals; 6.7 g/L Yeast nitrogen base without amino acids, Thermo Fisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Goat anti-Rabbit IgG (H+L)-HRP (Bio Rad, 1706515) and Goat anti-Mouse IgG (H+L)-HRP (Invitrogen, 31430) were used as secondary antibody for western blotting.
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bioRxiv - Bioengineering 2019Quote: ... were cultured in (DMEM containing D-glucose [1 g L-1] and sodium pyruvate [110 mg L-1] (Life Technologies), supplemented with penicillinstreptomycin [100 U mL-1] (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... media was changed to 500ul per well Opti-MEM reduced serum medium with HEPES 2.4g/L sodium bicarbonate and L-glutamine (Gibco #31985070). Scramble control siRNA-A (sc-37007) ...
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bioRxiv - Microbiology 2019Quote: ... CuSO4*5H2O 0.03 g/L and MnSO4*H2O 0.2 g/L) and with NaNO3 (Fisher Scientific Canada, Ottawa, ON, Canada) at various concentrations ...
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bioRxiv - Cell Biology 2020Quote: ... goat anti-rabbit Alexa Fluor 488 IgG H+L (1:400, A11008) and goat anti-mouse Alexa Fluor 568 IgG H+L (1:250, A11004) (Invitrogen, ThermoFisher).
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bioRxiv - Microbiology 2020Quote: ... Alexafluor 488 F(ab’)2 fragment of goat anti-rabbit IgG (H+L) (1:100) and Alexafluor 488 goat anti-mouse IgG (H+L) (1:100) were all purchased from ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... we collected three 1 L surface water samples into 1 L Nalgene wide-mouth HDPE bottles (ThermoFisher Scientific, Waltham, MA). Bottles were previously decontaminated by soaking in a 10% bleach solution for at least 10 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... Then the cells were washed with PBS and incubated for 1 hour with secondary antibody [Alexa Fluor-488 goat anti-mouse immunoglobulin G (IgG) (H+L) conjugate or anti-rabbit IgG (H+L) conjugate (Invitrogen)] ...
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bioRxiv - Cell Biology 2021Quote: ... 5% CO2 in minimal medium containing 1 part DMEM (4.5 g/L D-Glucose, Pyruvate, L-Glutamine; Invitrogen 11971-025): 1 part DMEM/F12 ...
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bioRxiv - Molecular Biology 2022Quote: HEK293T cells were cultured in Dulbecco’s modified Eagle medium (DMEM 1X, with 4.5 g/L D-glucose, + L-glutamine, - sodium pyruvate, Gibco 11965-092) and passaged using 0.25% trypsin in EDTA (Gibco 25200-056).
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bioRxiv - Bioengineering 2022Quote: ... and stable pAGDisplay-RBD cell lines were cultured at 37°C in a 5% CO2 atmosphere in DMEM medium (4.5 g/L glucose, L-glutamine; Gibco, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2019Quote: ... Alexa Fluor 488 and Alexa Fluor 568 conjugated Goat Anti-Rabbit IgG (H+L) and Anti-Mouse IgG (H+L) (Invitrogen) and Alexa Fluor 405 conjugated Goat Anti-Rabbit IgG (H&L ...
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bioRxiv - Synthetic Biology 2020Quote: ... HEK293T (ATCC CRL-3216), was cultured in complete media (DMEM; 1 g/l glucose, 2 mM L-glutamine, 10 % heat-inactivated FBS (Gibco)) with 5 % CO2 at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... 18.7 mM NH4Cl and 0.2 g/L glucose and supplemented with 20 mL/L MEM essential amino acids (Gibco 11130051), 10 mL/L MEM nonessential amino acids (Gibco 11140050) ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 594- or Alexa Fluor 647-conjugated highly cross-absorbed goat anti-mouse IgG (H+L) or goat anti-rabbit IgG (H+L) (Invitrogen), HRP-conjugated goat anti-rabbit or goat anti-mouse IgG (Jackson ImmunoResearch).
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bioRxiv - Immunology 2020Quote: ... Goat anti-Mouse IgG (H+ L) Alexa Fluor 555 and Goat anti-Mouse/Rabbit IgG (H+L) FITC was purchased from Invitrogen; Mouse anti-Flag/HA-tags beads was purchased from Abmart ...